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Effect of psychological stress on gastric motility assessed by electrical bio-impedance

World Journal of Gastroenterology 2012年第36期18卷 5027-5033页

作者： María Raquel Huerta-Franco Miguel Vargas-Luna Juana Berenice Montes-Frausto ismael morales-mata Lorena Ramirez-Padilla Department of Applied Sciences to Work Health Science DivisionUniversity of Guanajuato37320 LeonGuanajuatoMexico Department of Physical Engineering Science and Engineering DivisionUniversity of Guanajuato37150 LeonGuanajuatoMexico

AIM:To evaluate gastric motility using electrical bioimpedance(EBI) and gastric changes as a result of stress induced by psychological ***:A group of 57 healthy women,aged 40-60 years,was recruited,and a clinical history and physical examination were *** women were free from severe anxiety,chronic or acute stress,severe depression,mental diseases and conditions that affect gastric *** women were evaluated under fasting conditions,and using a four-electrode configuration,the gastric signals were obtained through a BIOPAC MP-150 *** volunteers were evaluated using the following paradigm:basal state,recording during the Stroop Test,intermediate resting period,recording during the Raven Test,and a final resting *** analyzed the relative areas of the frequency spectrum:A1(1-2 cpm),A2(2-4 cpm),A3(4-8 cpm),and A4(8-12 cpm),as well as the median of area A2 + *** data were analyzed by an autoregressive method using a Butterworth filter with MatLab and *** of variance(ANOVA) and Friedman ANOVA(for nonparametric variables) were performed;in addition,pairs of groups were compared using the T dependent and Wilcoxon T ***:The results of the main values of area A2 were not significantly different comparing the five steps of the experimental ***,there was a tendency of this A2 region to decrease during the stress tests,with recuperation at the final resting *** an extended gastric region was considered(1-4 cpm),significant differences with the psychological stress tests were present(F = 3.85,P = 0.005).The A3 region also showed significant changes when the stress psychological tests were administered(F = 7.25,P < 0.001).These differences were influenced by the changes in the adjacent gastric region of *** parameter that we proposed in previous studies for the evaluation of gastric motility by electrical bio-impedance(EBI) was the median of the area under the region from 2 to 8 cpm(A2 + A3)

关键词： Psychological stress Bio-impedance Gas-tric motility

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Effects of metoclopramide on gastric motility measured by short-term bio-impedance

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World Journal of Gastroenterology 2009年第38期15卷 4763-4769页

作者： María-Raquel Huerta-Franco Miguel Vargas-Luna Kathleen M Capaccione Etna Yaez-Roldán Ulises Hernández-Ledezma ismael morales-mata Teodoro Córdova-Fraga Department of Applied Science and Labor Research DCS-Campus LeónUniversity of Guanajuato Department of Physical Engineering DCI-Campus LeónUniversity of Guanajuato Robert Wood Johnson Medical School The University of Medicine and Dentistry of New Jersey

AIM:To analyze the accuracy of short-term bio-impedance as a means of measuring gastric ***: We evaluated differences in the short-term electrical bio-impedance signal from the gastric region in the following conditions: (1) fasting state, (2) after the administration of metoclopramide (a drug that induces an increase in gastric motility) and (3) after food ingestion in 23 healthy volunteers. We record-ed the real component of the electrical impedance signal from the gastric region for 1000 s. We performed a Fast Fourier Transform (FFT) on this data and then compared the signal among the fasting, medicated, and postprandial conditions using the median of the area under the curve, the relative area under the curve and the main peak ***: The median of the area under the curve of the frequency range in the region between 2-8 cycles per minute (cpm) decreased from 4.7 cpm in the fasting condition to 4.0 cpm in the medicated state (t=3.32, P=0.004). This concurred with the decrease seen in the relative area under the FFT curve in the region from 4 to 8 cpm from 38.3% to 26.6% (t=2.81, P=0.012) and the increase in area in the region from 2 to 4 cpm from 22.4% to 27.7%, respectively (t=-2.5, P=0.022). Finally the main peak position also decreased in the region from 2 to 8 cpm. Main peak activity in the fasting state was 4.72 cpm and declined to 3.45 cpm in the medicated state (t=2.47, P=0.025). There was a decrease from the fasting state to the postprandial state at 3.02 cpm (t=4.0, P=0.0013). CONCLUSION: Short-term electrical bio-impedance can assess gastric motility changes in individuals experiencing gastric stress by analyzing the area medians and relative areas under the FFT curve.

关键词： Bio-impedance Fast Fourier Transform Gastric motility Metoclopramide Postprandial

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Electrical bioimpedance and other techniques for gastric emptying and motility evaluation

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World Journal of Gastrointestinal Pathophysiology 2012年第1期3卷 10-18页

作者： María Raquel Huerta-Franco Miguel Vargas-Luna Juana Berenice Montes-Frausto Corina Flores-Hernández ismael morales-mata Health Science Division Department of Applied Sciences to Work University of Guanajuato Av Eugenio Garza Sada 572 Lomas del Campestre 37150 León Guanajuato México Science and Engineering Division Department of Physical Engineering University of Guanajuato Loma del Bosque 103 Lomas del Campestre 37150 León Guanajuato México

The aim of this article is to identify non-invasive, inexpensive, highly sensitive and accurate techniques for evaluating and diagnosing gastric diseases. In the case of the stomach, there are highly sensitive and specific methods for assessing gastric motility and emptying (GME). However, these methods are invasive, expensive and/or not technically feasible for all clinicians and patients. We present a summary of the most relevant international information on non-invasive methods and techniques for clinically evaluating GME. We particularly emphasize the potential of gastric electrical bioimpedance (EBI). EBI was initially used mainly in gastric emptying studies and was essentially abandoned in favor of techniques such as electrogastrography and the gold standard, scintigraphy. The current research evaluating the utility of gastric EBI either combines this technique with other frequently used techniques or uses new methods for gastric EBI signal analysis. In this context, we discuss our results and those of other researchers who have worked with gastric EBI. In this review article, we present the following topics: (1) a description of the oldest methods and procedures for evaluating GME; (2) an explanation of the methods currently used to evaluate gastric activity; and (3) a perspective on the newest trends and techniques in clinical and research GME methods. We conclude that gastric EBI is a highly effective non-invasive, easy to use and inexpensive technique for assessing GME.

关键词： Gastrointestinal motility Gastric emptying Bioimpedance technique Diagnostic techniques Digestive system

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Distributed Differences Structures Underlie Gating between the Kin Channel KAT1 and the Kout Channel SKOR

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Molecular Plant 2010年第1期3卷 236-245页

作者： Janin Riedelsberger Tripti Sharma Wendy Gonzalez Pawel Gajdanowicz Samuel Elias morales-Navarro Carlos Garcia-mata Bernd Mueller-Roeber Fernando Danilo Gonzalez-Nilo Michael R. Blatt Ingo Dreyer Universitat Potsdam Institut for Biochemie und Biologie Molekularbiologie Heisenberg-Gruppe Biophysik und Molekulare Pflanzenbiologie BPMPB Karl-Liebknecht-Strasse 24-25 Haus 20 D-14476 Potsdam-Golm Germany Max-Planck Institute of Molecular Plant Physiology 14476 Potsdam-Golm Germany Centro de Bioinformatica y Simulacion Molecular Universidad de Talca Casilla 721 Talca Chile Laboratory of Plant Physiology and Biophysics IBLS Plant Sciences Bower Building University of Glasgow Glasgow G12 8QQ UK Laboratorio de Fisiologia Molecular e Integrativa Institutos de Investigaciones Biologicas Universidad Nacional de Mar del Plata 7600 Mar del Plata Buenos Aires Argentina Universitat Potsdam Institut for Biochemie und Biologie Abteilung Molekularbiologie 14476 Potsdam-Golm Germany

The family of voltage-gated （Shaker-like） potassium channels in plants includes both inward-rectifying （Kin） channels that allow plant cells to accumulate K＋ and outward-rectifying （Kout） channels that mediate K＋ efflux. Despite their dose structural similarities, Kin and Kout channels differ in their gating sensitivity towards voltage and the extracellular K＋ concentration. We have carried out a systematic program of domain swapping between the Kout channel SKOR and the Kin channel KAT1 to examine the impacts on gating of the pore regions, the S4, S5, and the S6 helices. We found that, in particular, the N-terminal part of the S5 played a critical role in KAT1 and SKOR gating. Our findings were supported by molecular dynamics of KAT1 and SKOR homology models. In silico analysis revealed that during channel opening and closing, displacement of certain residues, especially in the S5 and S6 segments, is more pronounced in KAT1 than in SKOR. From our analysis of the S4-S6 region, we conclude that gating （and K＋-sensing in SKOR） depend on a number of structural elements that are dispersed over this -145-residue sequence and that these place additional constraints on configurational rearrangement of the channels during gating.

关键词： Arabidopsis K+ channel outward rectifier inward rectifier channel protein structure channel protein-cation interaction gating, K+-dependent.

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Hexavalent Chromium Removal by Citrus limonium Shell

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Open Journal of Inorganic Non 2012年第3期2卷 19-24页

作者： Juan Manuel Vargas-morales Diana Bautista-mata Juan F. Cárdenas-González Víctor Manuel Martínez-Juárez ismael Acosta-Rodríguez área Académica de Medicina Veterinaria y Zootecnia Instituto de Ciencias AgropecuariasUniversidad Autónoma del Estado de Hidalgo Tulancingo de Bravo México Laboratorio de Micología Experimental Centro de Investigación y de Estudios de Posgrado Facultad de Ciencias Químicas Universidad Autónoma de San Luis Potosí San Luis Potosí México

We studied the Chromium(VI) removal capacity in aqueous solution by the lemon shell, using the diphenylcarbazide method to evaluate the metal concentration. So, the highest biosorption of the metal (50 mg/L) occurs within 100 minutes, at pH of 1.0, and 28°C. According to temperature, the highest removal was observed at 60°C, in 11 minutes, when the metal (1 g/L) is completely adsorbed. At the analyzed concentrations of Cr(VI), lemon shell, showed excellent removal capacity, besides it removes efficiently the metal in situ (97.2% removal, 7 days of incubation, 5 g of biomass). After 1 hour of incubation the studied biomass reduces 1.0 g of Cr(VI) with the simultaneous production of Cr(III);so it can be used to eliminate it from industrial wastewater.

关键词： Chromium(VI) Biosorption Remotion Biomass Chromium(III)

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墨西哥帕丘卡卵胎生条纹强棱蜥的繁殖周期(英文)

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动物学报 2005年第6期51卷 998-1005页

作者： Aurelio RAM■REZ-BAUTISTA Carlos A.MACIEL-mata Miguel Angel MART■NEZ-morales Centro de Investigaciones Biológicas Universidad Autónoma del Estado de HidalgoA.P.1-69 Plaza JuárezC.P.42001 Pachuca HidalgoMéxico

本文报道墨西哥伊达尔戈州帕丘卡地区干旱环境中卵胎生条纹强棱蜥的繁殖周期及特征。测定墨西哥两个科学收藏馆的浸制标本获得数据。雌雄个体达到性成熟时的体长相等,为40.0mm。雄体睾丸重量在5月开始增加,至7、8月达到最大。睾丸重量... 详细信息

本文报道墨西哥伊达尔戈州帕丘卡地区干旱环境中卵胎生条纹强棱蜥的繁殖周期及特征。测定墨西哥两个科学收藏馆的浸制标本获得数据。雌雄个体达到性成熟时的体长相等,为40.0mm。雄体睾丸重量在5月开始增加,至7、8月达到最大。睾丸重量变化与降雨量、温度和光周期均无关。雌体卵泡重量在7月开始增加,并一直持续至10月。从11月至次年7月,雌体怀有胚胎。卵黄发生及胚胎发育与光周期、温度和降雨量无关。窝仔数与雌体体长呈正相关。平均窝仔数为5.2±0.2个(2-10个)。

关键词：条纹强棱蜥角蜥科繁殖周期帕丘卡伊达尔戈墨西哥

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Determination of the Biodiesel Content in Petrodiesel/Biodiesel Blends: A Method Based on Uv-Visible Spectroscopy and Chemometrics Tools

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American Journal of Analytical Chemistry 2013年第6期4卷 273-276页

作者： Armando Guerrero Francisco Anguebes Mepivoseth Castelán Victorino morales ismael León José C. Zavala Atl V. Córdova Campus Córdoba Colegio de Postgraduados Carr. Fed. Córdoba-Veracruz Amatlán de los Reyes Córdoba México Campus Tabasco Colegio de Postgraduados Córdoba México Facultad de Química Universidad Autónoma del Carmen Av. Concordia Col. Benito Juárez Ciudad del Carmen Ciudad del Carmen México Universidad Autónoma del Estado de Morelos Centro de Investigaciones Químicas (CIQ) Av. Universidad Cuernavaca Cuernavaca México

In this work, we developed an analytical method based on UV-visible spectroscopy to determine the concentration of biodiesel from African palm in blends of petrodiesel. Seventy-five samples with biodiesel concentrations between 0-100 wt% were prepared. The spectral fingerprints that were obtained from the analysis of the samples by UV-visible spectroscopy were used to build predictive model using PLS regression. The predictive ability of the models was evaluated through statistical parameters: the standard error of calibration (SEC), the standard error of validation (SEV), the correlation coefficient of calibration (r Cal) and validation (r Val), the ratio (SEC/SEV), the coefficient of determination R2, the paired data Student’s t-test, cross-validation and external validation. The results indicate that the PLS model predicts the concentration of biodiesel from African palm with high precision in mixtures with petrodiesel. The method developed in this study can be applied to determine the concentration of biodiesel African palm in mixtures of petrodiesel in a more rapid and economical way. Moreover, this method has less analytical errors and is more environmentally friendly than the conventional methods.

关键词： African Palm Petrodiesel/Biodiesel Blends Regression Model PLS Spectroscopy UV-Visible

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Induction of Extracellular Lytic Enzymes by <i>Fusarium solani</i>

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Advances in Microbiology 2013年第8期3卷 24-30页

作者： María de Guadalupe Moctezuma-Zárate Juan Manuel Vargas-morales Juan F. Cárdenas-González Víctor Manuel Martínez-Juárez ismael Acosta-Rodríguez área Académica de Medicina Veterinaria y Zootecnia Instituto de Ciencias Agropecuarias Universidad Autónoma del Estado de Hidalgo Tulancingo de Bravo Hidalgo México Laboratorio de Micología Experimental Centro de Investigación y de Estudios de Posgrado Facultad de Ciencias Químicas Universidad Autónoma de San Luis Potosí San Luis Potosí México

Fusarium solani is a necrotrophic parasitic fungus that causes wilt in some plants, causing severe economic losses in some areas of the country. The objective of this work was to analyze the induction of extracellular lytic enzymes produced by a strain of F. solani, isolated from a culture of tomato, in Villa de Arista, S.L.P. México. Polygalacturonase activity has a greater induction time at 10 days, and the xylanase has two times higher activity at 8 and 13 days of incubation at 28?C. Also, the xylanase activities A and B were very stable at 4?C. After 7 days of incubation, it has an activity of 100% and 96%, respectively, while polygalacturonase retains 61% of its initial activity. Both activities are better induced with glutamate and urea as nitrogen sources respectively, and both exhibit an initial pH optimum of 5.5. Finally, we didn’t find cellulase activity in the analyzing conditions.

关键词： Fusarium solani Necrotrophic Fungus Lytic Enzymes

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Digital Intubation without Stylet: Myth or Reality? Case Report

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Open Journal of Anesthesiology 2021年第5期11卷 156-163页

作者： Maya Christiam Mauricio Jesús Alberto Melendez Ordoñez ismael Bernardo Viveros Peralta Villanueva Verónica Juan José Dosta Herrera Andrea Carolina Jimenez Palacios Jimmy Andersson Delgado Criollo Monserrat Escalante Rodríguez Dalia Fernanda Farrera Rámirez Maribel Méndez Suarez Pedro Sánchez mata Residente de Anestesiología Hospital Juárez de México Ciudad de México México Médico adjunto al Servicio de Anestesiología Unidad Médica de Alta Especialidad (UMAE) Hospital General Doctor Gaudencio Jefe del Servicio de Anestesiología Unidad Médica de Alta Especialidad (UMAE) Hospital General Doctor Gaudencio González Garza (LA RAZA) Ciudad de México México

Digital intubation was discovered as one of the first methods to face a difficult airway without direct laryngoscopy. From the very beginning, this technique has been surrounded by much controversy, mainly because it required to be performed by an expert. Nowadays, it remains a useful technique when treating patients with difficult airways, so it is of utmost importance all personnel involved with airway management must know and perfect this technique when scenarios where conventional laryngoscopy or rescue devices for difficult airway are not available or contraindicated. The present work’s main objective is to suggest digital intubation as a safe and effective technique for the management of patients with difficult airways when there are no other devices available. The authors present a successful case of digital intubation on a patient with a difficult airway, demonstrating this technique is useful when performed by expert practitioners and when there is no other equipment available.

关键词： Intubation Intratracheal Oxygenation Anesthesia Airway Management

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