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Comparison of reversed-phase enantioselective HPLC methods for determining the enantiomeric purity of (S)-omeprazole in the presence of its related substances

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Journal of Pharmaceutical Analysis 2016年第2期6卷 132-136页

作者： Bruno Gallinella Rosella Ferretti Leo Zanitti isabella sestili Antonina Mosca Roberto Cirilli Istituto Superiore di Sanita Dipartimento del Farmaco Viale Regina Elena 299 1-00161 Rome Italy

A simple analytical high-performance liquid chromatography （HPLC） method was applied for the en- antiomeric excess determination of esomeprazole （（S）-OME）, the enantiopure active ingredient con- tained in drug products, in the presence of its potential organic impurities A-E. The enantioselective separation was accomplished on the immobilized-type Chiralpak ID-3 chiral stationary phase （CSP） under reversed-phase conditions. The results were evaluated and compared with those obtained by the official enantioselective method of European Pharmacopoeia used as the reference for checking the enantiomeric excess of （S）-OME. It has been established that the use of the Chiralpak ID-3 CSP allows the determination of the enantiomeric purity of （S）-OME without any interference coming from its chiral and achiral related substances. The analytical procedure of the drug regulatory agencies based on the AGP CSP suffered instead from poor specificity due to overlap of the peaks pertinent to the achiral impurity A and the chiral impurity （R）-OME （impurity F）.

关键词： Chiralpak ID-3 Enantiomers Enantioselective HPLC Impurities(S)-Omeprazole

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ddRAD sequencing-based genotyping for population structure analysis in cultivated tomato provides new insights into the genomic diversity of Mediterranean‘da serbo’type long shelf-life germplasm

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Horticulture Research 2020年第1期7卷 1047-1060页

作者： Salvatore Esposito Teodoro Cardi Gabriele Campanelli Sara sestili María JoséDíez Salvador Soler Jaime Prohens Pasquale Tripodi CREA Research Centre for Vegetable and Ornamental Crops Pontecagnano(SA)Italy CREA Research Centre for Vegetable and Ornamental Crops Monsampolo del Tronto(AP)TrontoItaly Instituto de Conservación y Mejora de la Agrodiversidad Valenciana Universitat Politècnica de València46022 ValenciaSpain

Double digest restriction-site associated sequencing(ddRAD-seq)is a flexible and cost-effective strategy for providing in-depth insights into the genetic architecture of germplasm *** this methodology,we investigated the genomic diversity of a panel of 288 diverse tomato(Solanum lycopersicum L.)accessions enriched in‘da serbo’(called‘de penjar’in Spain)long shelf life(LSL)materials(152 accessions)mostly originating from Italy and *** rest of the materials originate from different countries and include landraces for fresh consumption,elite cultivars,heirlooms,and breeding *** from their LSL trait,‘da serbo’landraces are of remarkable interest for their *** identified 32,799 high-quality SNPs,which were used for model ancestry population structure and non-parametric hierarchical *** genetic subgroups were revealed,clearly separating most‘da serbo’landraces,but also the Spanish germplasm,suggesting a subdivision of the population based on type and geographical *** disequilibrium(LD)in the collection decayed very rapidly within<5 *** then investigated SNPs showing contrasted minor frequency allele(MAF)in‘da serbo’materials,resulting in the identification of high frequencies in this germplasm of several mutations in genes related to stress tolerance and fruit maturation such as CTR1 and ***,a mini-core collection of 58 accessions encompassing most of the diversity was selected for further exploitation of key *** findings suggest the presence of a genetic footprint of the‘da serbo’germplasm selected in the Mediterranean ***,we provide novel insights on LSL‘da serbo’germplasm as a promising source of alleles for tolerance to stresses.

关键词： germplasm breeding shelf

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