检索结果-南通市图书馆

Chromatin condensation but not DNA integrity of pig sperm is greater in the sperm-rich fraction

Journal of Animal Science and Biotechnology 2024年第1期15卷 171-181页

作者： Estel Viñolas-Vergés Jordi Ribas-Maynou isabel.barranco Camila Peres Rubio Sergi Bonet Jordi Roca Marc Yeste Unit of Cell Biology Department of BiologyFaculty of SciencesUniversity of GironaGironaSpain Biotechnology of Animal and Human Reproduction(TechnoSperm) Institute of Food and Agricultural TechnologyUniversity of GironaGironaSpain Department of Medicine and Animal Surgery Faculty of Veterinary ScienceUniversity of MurciaMurciaSpain Catalan Institution for Research and Advanced Studies(ICREA) S08010 BarcelonaSpain

Background Protamination and condensation of sperm chromatin as well as DNA integrity play an essential role during fertilization and embryo *** some mammals,like pigs,ejaculates are emitted in three separate fractions:pre-sperm,sperm-rich(SRF)and post sperm-rich(PSRF).These fractions are known to vary in volume,sperm concentration and quality,as well as in the origin and composition of seminal plasma(SP),with differences being also observed within the SRF ***,whether disparities in the DNA integrity and chromatin condensation and pro-tamination of their sperm exist has not been *** This study determined chromatin protamination(Chromomycin A3 test,CMA_(3)),condensation(Dibromobi-mane test,DBB),and DNA integrity(Comet assay)in the pig sperm contained in the first 10 m L of the SRF(SRF-P1),the remaining portion of the sperm-rich fraction(SRF-P2),and the post sperm-rich fraction(PSRF).While chromatin protamination was found to be similar between the different ejaculate fractions(P>0.05),chromatin condensation was seen to be greater in SRF-P1 and SRF-P2 than in the PSRF(P=0.018 and P=0.004,respectively).Regarding DNA integrity,no differences between fractions were observed(P>0.05).As the SRF-P1 has the highest sperm concentra-tion and ejaculate fractions are known to differ in antioxidant composition,the oxidative stress index(OSi)in SP,calcu-lated as total oxidant activity divided by total antioxidant capacity,was tested and confirmed to be higher in the SRF-P1 than in SRF-P2 and PSRF(0.42±0.06 vs.0.23±0.09 and 0.08±0.00,respectively;P<0.01);this index,in addition,was observed to be correlated to the sperm concentration of each fraction(Rs=0.973;P<0.001).Conclusion While sperm DNA integrity was not found to differ between ejaculate fractions,SRF-P1 and SRF-P2 were observed to exhibit greater chromatin condensation than the *** could be related to the OSi of each fraction.

关键词： Chromatin Condensation DNA integrity Ejaculate fractions Pig Protamination Sperm

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Phase-driven progress in nanophotonic biosensing

引用

Light(Science & Applications) 2024年第5期13卷 798-800页

作者： isabel Barth Hakho Lee Center for Systems Biology Massachusetts General HospitalHarvard Medical SchoolBostonMA 02114USA

In the continuous pursuit of enhancing the sensitivity of nanophotonic biosensors by leveraging phase phenomena,a recent development involved the engineering of an atomically thin Ge2Sb2Te5 layer on a silver nanofilm ... 详细信息

关键词： silver Ge2Sb2Te5 pursuit

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Evaluation of the efficiency of urban solid waste management in Brazil by data envelopment analysis and possible variables of influence

引用

Waste Disposal and Sustainable Energy 2024年第2期6卷 283-295页

作者： isabel das Merces Costa Marta Ferreira Dias Margarita Robaina GOvCOPP-Research Unit on Governance Competitiveness and Public Policies and Analyst of UniversidadeEstadual de Feira de SantanaUniversidade de AveiroAveiroPortugal GOvCOPP-Research Unit on Governance Competitiveness and Public PoliciesDEGEIT-Department of EconomicsManagementIndustrial Engineering and TourismUniversidade de AveiroAveiroPortugal

This article has the general objective of estimating the efficiency of urban solid waste management in 940 Brazilian munici-palities through Data Envelopment Analysis(DEA)technique and has specific objectives:(i)to estimate efficiency scores;(ii)to compare the performance between different groups of municipalities;and(ii)to analyze the profile of efficient munici-palities from the perspective of the guidelines of Law 12,305/2010 and socio-economic and environmental *** technique used was DEA with output-oriented and variable scale to return *** results showed higher efficiency scores in the municipalities with populations above 500,000 *** score variation ranged from 0.5(municipalities with populations<10,000 inhabitants)to 0.9(municipalities with more than 500,000 inhabitants).Of the sample set,only 12.34%of the municipalities were considered efficient,and when analyzing the efficient group,it was found that adherence to legislation was not a major factor in achieving efficiency.

关键词： Urban solid waste management Data envelopment analysis Efficiency Brazil Municipalities

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

The potential of starch-chitosan blends with poloxamer for the preparation of microparticles by spray-drying

引用

Particuology 2024年第6期89卷 1-10页

作者： Juan Enrique barranco-García Carolina Caicedo Enrique Javier Jiménez-Regalado Carlos Espinoza-González Graciela Morales Rocio Yaneli Aguirre-Loredo Abril Fonseca-García Tecnológico Nacional de México/Instituto Tecnológico de Oaxaca Departamento de Química y BioquímicaAvenida Ing.Víctor Bravo Ahuja No.125Esquina Calzada Tecnológico68030OaxacaMexico Faculty of Engineering Unidad Central del Valle del Cauca(UCEVA)Carrera 17a 48-144763022TuluáColombia Centro de Investigación en Química Aplicada(CIQA) Blvd.Enrique Reyna Hermosillo 14025294SaltilloCoahuilaMexico Investigadoras por México CONAHCYT-CIQA Av.Insurgentes Sur 1562Col.Crédito Constructor03940Alcaldía Benito JuárezCDMXMexico

Using biopolymers as wall materials in spray drying poses challenges,particularly in attaining flowability and thermal stability among their physicochemical *** paper addresses these challenges by preparing microparticles using a blend of starch–chitosan and a poloxamer,commercially named Pluronic®*** aimed to elucidate the effects of varying poloxamer concentrations on the resulting particles through the spray drying *** with a poloxamer concentration of 3%(w/v)demonstrated a notably higher yield,especially when compared to those with 0%and 1%*** with 3%and 5%(w/v)poloxamer displayed a narrower particle size distribution,with the 3%blend showing a superior yield attributed to arrangements of blend components that improve flowability.X-ray diffraction analysis showcased the characteristic peaks of A-type starch form,with shifts suggesting enhanced interactions between *** with increased poloxamer content showed elevated thermal degradation temperatures,with the 3%blend registering a significant rise,opening avenues for encapsulating heat-sensitive *** study primarily focuses on the preparation and basic characterization of *** underscores the potential of blends with optimal poloxamer concentrations in microencapsulation,emphasizing further research to harness their capabilities thoroughly.

关键词： Biopolymer Starch Chitosan Pluronic®F127 Microparticles Spray drying

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Towards efficient strain engineering of 2D materials:A four-points bending approach for compressive strain

引用

Nano Research 2024年第6期17卷 5317-5325页

作者： Hao Li Félix Carrascoso Ana Borrás Gloria P.Moreno Francisco J.Aparicio Ángel barranco Andrés Castellanos Gómez Campus de Cantoblanco Instituto de Ciencia de Materiales de MadridC.Sor Juana Inés de la Cruz328049 MadridSpain Nanotechnology on Surfaces and Plasma Lab Instituto de Ciencia de Materiales de Sevilla(CSIC-Universidad de Sevilla).C/Americo Vespucio 49E-41092 SevilleSpain Departamento de Física Aplicada I Escuela Politécnica SuperiorUniversidad de Sevillac/Virgen deÁfrica 7E-41011 SevilleSpain

Strain engineering,as a powerful strategy to tune the optical and electrical properties of two-dimensional(2D)materials by deforming their crystal lattice,has attracted significant interest in recent years.2D materials can sustain ultra-high strains,even up to 10%,due to the lack of dangling bonds on their surface,making them ideal brittle *** remarkable mechanical resilience,together with a strong strain-tunable band structure,endows 2D materials with a broad optical and electrical response upon ***,strain engineering based on 2D materials is restricted by their nanoscale and strain quantification *** this study,we have modified a homebuilt three-points bending apparatus to transform it into a four-points bending apparatus that allows for the application of both compressive and tensile strains on 2D *** approach allows for the efficient and reproducible construction of a strain system and minimizes the buckling effect caused by the van der Waals interaction by adamantane encapsulation *** results demonstrate the feasibility of introducing compressive strain on 2D materials and the potential for tuning their optical and physical properties through this approach.

关键词： uniaxial compressive strain polymer encapsulation differential reflectance spectroscopy exciton tunability

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

A test of the species confidence hypothesis in dusky damselfish

引用

Current Zoology 2024年第1期70卷 79-86页

作者： Elle Overs Sydney Stump isabel Severino Daniel T.Blumstein Department of Ecology and Evolutionary Biology University of CaliforniaLos AngelesCA 90095-1606USA

Visual cues are important in both interspecific and intraspecific *** species confidence hypothesis proposes that animals are more attracted to conspecific colors and repelled by colors,not on their *** on terrestrial lizards and birds have tested the species confidence hypothesis and shown that conspecific colors elicit reduced antipredator *** date,the species confidence hypothesis has not been tested in the marine environment,specifically on coral reefs where color communication is of vital *** addressed this knowledge gap by measuring flight initiation distance(the distance an individual moves away from an approaching threat)in dusky damselfish(Stegastes nigricans)in response to an approaching disc of 1 of 4 different color treatments:conspecific,blue,yellow,and *** the species confidence hypothesis explained variation in damselfish flight initiation distance,then we expected individuals to tolerate closer approaches when approached by a conspecific *** addition,we calculated the color difference between each stimulus and its corresponding back-ground as a potential alternative explanation for flight *** tolerated the closest approach from the conspecific color stimulus;there were no significant differences between other colors and there was no support for the alternative color difference *** with similar terrestrial studies,these results are relevant to ecotourists'choice of swimsuit and wetsuit colors because color choice may modify naturalantipredatorbehavior.

关键词： antipredator behavior flight initiation distance risk assessment species confidence hypothesis Stegastes nigricans

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Remediation of amide pesticide-polluted soils by combined solarization and ozonation treatment

引用

Pedosphere 2024年第3期34卷 641-651页

作者： isabel GARRIDO Carmen MMARTINEZ Pilar FLORES Pilar HELLIN Fulgencio CONTRERAS Jose FENOLL Department of Sustainability and Quality Group of Fruit and Vegetable Products Instituto Murciano de Investigacion y Desarrollo Agrario y MedioambientalLa Alberca 30150(Spain)

Agriculture has a close relationship with nature,but it can also be the source of negative and permanent environmental *** use of pesticides in modern agriculture is a common practice,but their side effects on the environment cannot be *** this study,we evaluated a combination of solarization and ozonation techniques for the elimination of six amide pesticides(boscalid,chlorantraniliprole,cyflufenamid,fluopyram,napropamide,and propyzamide)in *** experiments were performed with four different soils to assess the efficiency of this methodology at different soil temperatures and ozone dosages under laboratory conditions,and then a greenhouse pot experiment was conducted under controlled conditions during *** days after the onset of the experiments,higher degradation percentages of amide pesticides were observed in ozonized soils than in other treated soils,particularly when ozone was applied at 10 cm soil *** results show that the utilization of ozonation,along with solarization,represents a valid method for degrading residues of the studied pesticides and suggest that this combined technology may be a promising tool for remediating pesticide-polluted soils.

关键词： intermediate ozone pesticide degradation pesticide residue polyethylene film remediation technique

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Global black soil distribution

引用

Frontiers of Agricultural Science and Engineering 2024年第2期11卷 271-281页

作者： Yuxin TONG Marcos E.ANGELINI Yusuf YIGINI isabel LUOTTO Global Soil Partnership Land and Water DivisionFood and Agriculture Organization of the United NationsRome 00153Italy

Black soils, characterized by their thick, dark horizons enriched with organic matter, epitomize highly fertile soils. However, their fertility precipitates intense land use, engendering challenges such as soil erosion, nutrient depletion, pollution, compaction, salinization, and acidification. Notably, these soils are significant contributors to global greenhouse gas emissions, primarily due to substantial losses in soil organic carbon. Despite these challenges, black soils are pivotal for global food production. This paper delineates the implementation of digital soil mapping for the global cartography of black soils and human interference on these soils. Predominantly distributed in Eastern Europe, Central and Eastern Asia, and North and South America, black soils cover an approximate area of 725 Mha, with the Russian Federation,Kazakhstan, and China collectively have over half of this area. Agriculturally,these soils underpin significant proportions of global crop yields, producing 66% of sunflower, 30% of wheat, and 26% of potato outputs. The organic carbon content in the upper 30 cm of these soils is estimated at 56 *** management of black soils is imperative for ensuring food security and addressing climate change on a global scale.

关键词： Black soils distribution map food security soil organic carbon

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Two intestinal microbiota-derived metabolites, deoxycholic acid and butyrate, synergize to enhance host defense peptide synthesis and alleviate necrotic enteritis

引用

Journal of Animal Science and Biotechnology 2024年第3期15卷 1158-1172页

作者： Dohyung M.Kim Jing Liu Melanie A.Whitmore isabel Tobin Zijun Zhao Guolong Zhang Department of Animal and Food Sciences Oklahoma State UniversityStillwaterOKUSA

Background Necrotic enteritis(NE)is a major enteric disease in poultry,yet effective mitigation strategies remain *** acid(DCA)and butyrate,two major metabolites derived from the intestinal microbiota,have independently been shown to induce host defense peptide(HDP)***,the potential synergy between these two compounds remains *** To investigate the possible synergistic effect between DCA and butyrate in regulating HDP synthesis and barrier function,we treated chicken HD11 macrophage cells and jejunal explants with DCA and sodium butyrate(NaB),either individually or in combination,for 24 ***,we performed RNA isolation and reverse transcrip-tion-quantitative PCR to analyze HDP genes as well as the major genes associated with barrier *** further determine the synergy between DCA and NaB in enhancing NE resistance,we conducted two independent trials with Cobb broiler *** each trial,the diet was supplemented with DCA or NaB on the day-of-hatch,followed by NE induction through sequential challenges with Eimeria maxima and Clostridium perfringens on d 10 and 14,*** recorded animal mortality after infection and assessed intestinal lesions on d *** impact of DCA and NaB on the microbiota in the ileum and cecum was evaluated through bacterial 16S rRNA gene *** We found that the combination of DCA and NaB synergistically induced multiple HDP genes in both chicken HD11 cells and jejunal ***,the gene for claudin-1,a major tight junction protein,also exhibited synergistic induction in response to DCA and ***,dietary supplementation with a combination of 0.75 g/kg DCA and 1 g/kg NaB led to a significant improvement in animal survival and a reduction in intestinal lesions compared to either compound alone in a chicken model of ***,the cecal microbiota of NE-infected chickens showed a marked decrease in SCFA-producing bacteria such as Bacteroides,F

关键词： Bile acids Host defense peptides Metabolites Microbiome Necrotic enteritis Short-chain fatty acids

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Prevalence of visual impairment and estimation of refractive errors among school children in Kakamega,Kenya

引用

International Journal of Ophthalmology(English edition) 2024年第5期17卷 932-939页

作者： isabel Signes-Soler Alfred Ragot Sheilah Nangena Andrew Wekesa Raúl Montalbán Llamusí Department of Optics and Optometry and Vision Sciences Universidat de ValènciaBurjassotValencia 46010Spain NGO Vision Without Borders(Visio Sense Fronteres VSF)Calpe 03710AlicanteSpain Masinde Muliro University of Science and Technology P.O Box 190-50100KakamegaKenya Clinica Baviera Alicante C/Ausóy Monzó 16Alicante 03006Spain

AIM:To investigate the prevalence of visual impairment(VI)and provide an estimation of uncorrected refractive errors in school-aged children,conducted by optometry students as a community ***:The study was *** 3343 participants were included in the *** initial examination involved assessing the uncorrected distance visual acuity(UDVA)and visual acuity(VA)while using a+2.00 D *** inclusion criteria for a subsequent comprehensive cycloplegic eye examination,performed by an optometrist,were as follows:a UDVA<0.6 decimal(0.20 logMAR)and/or a VA with+2.00 D≥0.8 decimal(0.96 logMAR).RESULTS:The sample had a mean age of 10.92±2.13y(range 4 to 17y),and 51.3%of the children were female(n=1715).The majority of the children(89.7%)fell within the age range of 8 to *** the ethnic groups,the highest representation was from the Luhya group(60.6%)followed by Luo(20.4%).Mean logMAR UDVA choosing the best eye for each student was 0.29±0.17(range 1.70 to 0.22).Out of the total,246 participants(7.4%)had a full eye *** estimated prevalence of myopia(defined as spherical equivalent≤-0.5 D)was found to be 1.45%of the total *** around 0.18%of the total sample had hyperopia value exceeding+1.75 *** astigmatism(cil<-0.75 D)was found in 0.21%(7/3343)of the *** VI prevalence was 1.26%of the total *** our cases of VI,76.2%could be attributed to uncorrected refractive *** was detected in 0.66%(22/3343)of the screened *** was no statistically significant correlation observed between age or gender and refractive ***:The primary cause of VI is determined to be uncorrected refractive errors,with myopia being the most prevalent refractive error *** findings underscore the significance of early identification and correction of refractive errors in school-aged children as a means to alleviate the impact of VI.

关键词： visual impairment refractive errors myopia amblyopia sustainable development goals

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：