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Diatoms’ Breakthroughs in Biotechnology: <i>Phaeodactylum tricornutum</i>as a Model for Producing High-Added Value Molecules

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American Journal of Plant Sciences 2020年第10期11卷 1632-1670页

作者： Fadoua Dhaouadi Fatima Awwad Andrew Diamond isabel desgagné-penix Department of Chemistry Biochemistry and Physics Université du Québec à Trois-Rivières Boulevard des Forges Trois-Rivières Québec Canada

With a world growing in population and nutritional needs, diatoms are considered nowadays as microalgae of a very important potential, thus they are exploited in several fields such as ecology, aquaculture, molecular farming, and pharma nutraceuticals. These coveted microalgae are characterized by their diversity, their high division rates, their complex life cycle, likewise their silicified cell walls named frustules. Thus, diatoms have been used for over a century after proving an efficient production of several molecules including Triacylglycerols (TAGs), H2, free fatty acids, vitamins, nutraceuticals, amino acids, proteins, terpenoids, alcohols and carbohydrates like starch, glycogen, and sucrose. Phaeodactylum tricornutum is the most promising diatom exploited to date, especially as a platform of pharmaceutical production. Herein, we expose diatoms’ main features that allowed using them for molecular farming. This review exposes likewise, the metabolism and the post-translational modifications (PTMs) of diatoms as well as current tools and challenges for their molecular and metabolic engineering for a more efficient production of valuable molecules. The knowledge on the biology of the diatoms, the molecular tools, and the various transformation methods available demonstrate the potential in biotechnology of these photosynthetic microorganisms. The widely studied P. tricornutum, as a model organism, is a promising diatom for production of valuable metabolites, despite the challenges and issues related to cultivation.

关键词： Diatoms Silicified Frustules Metabolism Lipids Genetic Engineering Molecules

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Impact of Different Light Characteristics on the Growth and Lipid Content of Diatom Phaeodactylum tricornutum Transconjugant Strains

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American Journal of Plant Sciences 2023年第1期14卷 41-63页

作者： Nikunj Sharma Elisa Ines Fantino Fatima Awwad Natacha Mérindol Arun Augustine Fatma Meddeb isabel desgagné-penix Department of Chemistry Biochemistry and Physics Université du Québec à Trois-Rivières Trois-Rivières Québec Canada

Light regulates important metabolic processes in microalgal cells, which can further impact the metabolism and the accumulation of biomolecules such as lipids, carbohydrates, and proteins. Different characteristics of light have been studied on various strains of the model diatom Phaeodactylum tricornutum, but not on transconjugant cells and information on wild-type strains is still limited. Therefore, we studied the impact of different light characteristics such as spectral quality, light intensity and light shift on the growth, and the composition in lipids and fatty acids of P. tricornutum cells to provide a comprehensive context for future applications. Initially, we tested the impact of spectral quality and light intensity on P. tricornutum transformed with an episomal vector (Ptev), harboring the resistance gene Sh ble. Results indicated that Ptev cells accumulated more biomass and overall lipids in spectral quality Red 1 (R1: 34% > 600 nm > 66%) more effectively as compared to Red 2 (R2: 8% > 600 nm > 92%). It was also detected that cell granularity was higher in R1 as compared to R2. Furthermore, by testing two light intensities 65 μmol·m-2·s-1 and 145 μmol·m-2·s-1 light, it was observed that 145 μmol·m-2·s-1 led to an increase in growth trend, total biomass and lipid content. Combining spectral qualities and light intensities, we show that the lipid accumulation raised by 2.8-fold. Studying the light intensity and spectral quality allowed us to optimize the light conditions to R1 spectral quality and light intensity 145 μmol·m-2·s-1. These initial results showed that red light R1 at 145 μmol·m-2·s-1 was the best condition for biomass and total lipids accumulation in Ptev cells. Next, we further combined these two-light optimizations with a third light characteristics, i.e. light s

关键词： Phaeodactylum tricornutum Light Condition Episomal Vector Dia-toms Fatty Acids Biomass

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Impact of Different Light Characteristics on the Growth and Lipid Content of Diatom Phaeodactylum tricornutum Transconjugant Strains

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American Journal of Plant Sciences（美国植物学期刊） 2023年第1期

作者： Nikunj Sharma Elisa Ines Fantino Fatima Awwad Natacha Mérindol Arun Augustine Fatma Meddeb isabel desgagné-penix

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