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Optimization of the Production of Biogas in Anaerobic Digestion by the Addition of Hydrolytic Microorganisms

Journal of Environmental Science and Engineering(A) 2022年第1期11卷 8-12页

作者： María Montes Melissa Cervantes Alejandra Vásquez Víctor Aguilar Pedro García isabel del castillo Department of Environmental Engineering Institute for EngineeringUniversity of VeracruzBoca del RioVer.94294Mexico Department Chemical Engineering Faculty of Chemical SciencesUniversity of VeracruzBoca del RioVer.94294Mexico Department of Administration Open Teaching SystemUniversity of VeracruzBoca del RioVer.94294Mexico Department Sanitary Engineering E.T.S.I.Civil EngineeringPolytechnic University of MadridMadrid28040Spain

It is well known that fossil fuels are one of the major pollutants that contribute to climate change and there is a need to develop alternatives to their *** option is the use of a renewable fuel such as biogas obtained from the breaking down of organic matter in the anaerobic digestion *** biogas is cleaned up to a certain quality,it can substitute the use of natural ***,this slow process requires optimization to increase the amount of *** are chemical,physical and biological treatments for organic matter to increase the amount and quality of *** the former research,a biological treatment was tested by adding a selection of microorganisms that showed hydrolytic activity to an anaerobic digester for its ***,a selection of microorganisms with the ability to grow in fresh sewage sludge as only nutrient was ***,the lipolytic,cellulolytic and PA(Proteolytic Activity)of microorganisms was tested using selective growth ***,10 samples with the greatest hydrolytic activity were used to evaluate their effects in the increasing of biogas production in the anaerobic *** results showed that 83%of the microorganisms with the ability to grow in sewage sludge were proteolytic,while the percentage of lipolytic and cellulolytic microorganisms was ***,it was found that proteolytic microorganisms are the ones that increase biogas yield in a maximum of 80%with a COD(Chemical Oxygen Demand)reduction of 58%.

关键词： Hydrolytic microorganisms biogas anaerobic digestion

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Nuptial gifts and female fecundity in the neotropical katydid Conocephalus ictus （Orthoptera： Tettigonidae）

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Insect Science 2015年第1期22卷 106-110页

作者： isabel Ortiz-Jimenez Raul Cueva del castillo Laboratorio de Ecologia UBIPRO UNAM FES Iztacala M6xico Edo. Mexico M6xico

In general, female fitness is greatly increased in gift-giving insects. In katydids, this nuptial gift consists of a gelatinous mass produced by accessory glands： the spermato- phylax, which is attached to the ampulla. During mating, males of the neotropical katydid Conocephalus ictus transfer a spermatophylax that is ingested by the females. Fecundity, egg-laying rate and longevity were higher in females that consumed the spermatophylax than in those that did not. Also, female receptivity turned off after mating. Females actively rejected other males by hitting them with their forelegs and moving away. Their refractory period lasted as long as 17 d. Only a few females accepted a 2nd mating and died a few days later. In C. ictus, spermatophylax consumption can be beneficial for both males and females. On one hand, the compounds in the spermatophylax or the ejaculate could prevent or delay females from copulating with rivals, thus avoiding sperm competition. On the other hand, such compounds can improve the females＇ opportunity to increase their lifespan and fecundity. Moreover, a rise in egg-laying rate may lower the risk of female prereproductive death caused by rapid oviposition. In any case, the boost in female egg laying might also be beneficial for males because their number of offspring increases.

关键词： female fitness katydid neotropic spermatophylax

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Medicinal plants and natural products as neuroprotective agents in age-related macular degeneration

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Neural Regeneration Research 2020年第12期15卷 2207-2216页

作者： Francisco Bosch-Morell Victoria Villagrasa Teresa Ortega Nuria Acero Dolores Muñoz-Mingarro MEugenia González-Rosende Encarna castillo MAmparo Sanahuja Pilar Soriano isabel Martínez-Solís Biomedical Sciences Institute Universidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Biomedical Sciences Faculty of Health SciencesUniversidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Pharmacy Faculty of Health SciencesUniversidad Cardenal Herrera-CEUCEU UniversitiesAlfara del PatriarcaValenciaSpain Department of Pharmacology Pharmacognosy and BotanyUniversidad Complutense de MadridMadridSpain Department of Pharmaceutical and Health Sciences Faculty of PharmacyUniversidad San Pablo-CEUCEU UniversitiesMadridSpain Department of Chemistry and Biochemistry Faculty of PharmacyUniversidad San Pablo-CEUCEU UniversitiesMadridSpain ICBiBE-Botanical Garden University of ValenciaValenciaSpain

The retina may suffer neurodegenerative damages,as other tissues of the central nervous system do,and serious eye diseases may *** of them is age-related macular degeneration,which causes progressive loss of vision due to retina *** of age-related macular degeneration focuses on antioxidant agents and anti-vascular endothelial growth factor compounds,among others,that prevent/diminish oxidative stress and reduce neovascularisation *** phytochemicals,medicinal plants and/or plant-diet supplements might be a useful adjunct in prevention or treatment of age-related macular degeneration owing to their antioxidant and anti-vascular endothelial growth factor *** review article presents the most investigated plants and natural products in relation to age-related macular degeneration,such as saffron,ginkgo,bilberry and blueberry,curcuma or turmeric,carotenoids,polyphenols,and vitamins C and *** study provides up-to-date information on the effects,treatments,safety and efficiency of these phytotherapy products.

关键词： age-related macular degeneration bilberry blueberry curcuma carotenoids ginkgo polyphenols saffron vitamins

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Clinical-pathological and molecular characterization of long-term survivors with advanced non-small cell lung cancer

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Cancer Biology & Medicine 2020年第2期17卷 444-457页

作者： Juan Moreno-Rubio Santiago Ponce Rosa Alvarez Maria Eugenia Olmedo Sandra Falagan Xabier Mielgo Fatima Navarro Patricia Cruz Luis Cabezon-Gutierrez Carlos Aguado Gonzalo Colmenarejo Marta Munoz-Fernandez de Leglaria Ana Belen Enguita Maria Cebollero Amparo Benito isabel Alemany Carolina del castillo Ricardo Ramos Ana Ramirez de Molina Enrique Casado Maria Sereno Ana Ramirez de Molina1 Enrique Casado5Maria Sereno5 XIMDEA Food InstituteCEI UAM+CSICMadrid 28702Spain 12 de Octubre University Hospital Madrid 28041Spain Gregorio Maranon University Hospital Madrid 28009Spain Ramon y Cajal University Hospital Madrid 28034Spain Infanta Sofia University Hospital San Sebastian De Los ReyesMadrid 28702Spain Fundacion Alcorcon University Hospital AlcorconMadrid 28922Spain Prlncipe de Asturias University Hospital Alcala De HenaresMadrid 28805Spain La Paz University Hospital Madrid 28046Spain University Hospital of Torrejon Torrejon De ArdozMadrid 28850Spain Clinico San Carlos University Hospital Madrid 28040Spain Parque Cientifico de Madrid Foundation Madrid 28049Spain

Objective:Long-term survivors(LS)of non-small cell lung cancer(NSCLC)without driver alterations,displaying an overall survival(OS)of more than 3 years,comprise around 10%of cases in several series treated with *** are classical prognosis factors for these cases[stage,Eastern Cooperative Oncology Group(ECOG),etc.],but more data are required in the *** this multi-center study,we focused on LS of advanced NSCLC with OS above 36 months to perform a clinical-pathological and molecular ***:In the first step,we conducted a clinical-pathological characterization of the ***,we carried out a genetic analysis by comparing LS to a sample of short-term survivors(SS)(with an OS less than 9 months).We initially used whole-genome RNA-seq to identify differentiating profiles of LS and SS,and later confirmed these with reverse transcription-polymerase chain reaction(RT-PCR)for the rest of the ***:A total of 94 patients were included,who were mainly men,former smokers,having adenocarcinoma(AC)-type NSCLC with an ECOG of *** obtained an initial differential transcriptome expression,displaying 5 over-and 33 under-expressed genes involved in different pathways:namely,the secretin receptor,surfactant protein,trefoil factor 1(T FF1),serpin,Ca-channels,and Tolllike receptor(TLRs)***,RT-PCR analysis of 40(20 LS/20 SS)samples confirmed that four genes(surfactant proteins and SFTP)were significantly down-regulated in SS compared to LS by using an analysis of covariance(ANCOVA)model:SFTPA1(P=0.023),SFTPA2(P=0.027),SFTPB{P=0.02),and SFT PC(P=0.047).Conclusions:We present a sequential genetic analysis of a sample of NSCLCLS with no driver alterations,obtaining a differential RNA-seq/RT-PCR profile showing an abnormal expression of SF genes.

关键词： Long-term survivors non-small cell lung cancer surfactant proteins

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Utility of C-Reactive Protein and Procalcitonin for Detecting Bloodstream Infection in Patients with HIV/AIDS

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World Journal of AIDS 2014年第3期4卷 287-292页

作者： Arianna castillo Marshall Tersilia García Castellanos isabel Martínez Motas Daniel Salazar Rodriguez María Eugenia Toledo Romaní Jorge Pérez ávila Clinical Laboratory International Health Center La Pradera La Havana Cuba Department of Clinical Microbiology Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Epidemiology Research Department Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Institute of Tropical Medicine “Pedro Kourí” La Havana Cuba Medicine Faculty Latin-American’s School La Havana Cuba

Human immunodeficiency virus (HIV) infected persons, are at high risk for developing a bloodstream infection. In order to evaluate the usefulness of C-reactive protein (CRP) and procalcitonin (PTC) in the detection of bloodstream infection in HIV, a case-control study was conducted from February to December 2012. PCT and CPR levels were measured in 2 groups. PCT concentrations were measured by the VIDAS®?Brahms PCT assay, and CRP concentrations were determined by CRP latex. Values were calculated for both biomarkers and discriminative ability of PCT and CRP was analyzed using ROC curves. There were no significant differences between the study group and the control groups with respect to CRP levels. However, they were much higher PCT levels in patients with bacteremia. PCT showed greater discriminating ability compared to CRP, and proved to be a valuable tool for the detection of systemic bacterial infections in HIV infected patients.

关键词： Acute Renal Injury Surgical Hemostasis Absorbable Implants Inflammation Biocompatible

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Effect of the Substitution of Sand by Rubber of Waste Tires on the Mechanical Properties of Hydraulic Concrete and Exposure to Gamma Radiation

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Journal of Minerals and Materials Characterization and Engineering 2021年第3期9卷 245-256页

作者： Jesús M. Colín de la Cruz Carmen Gabriela Guzmán Fermín castillo Mejía Benjamin Leal Acevedo Osvaldo Flores Cedillo isabel Gamboa de Buen Arturo Molina Ocampo Horacio Martínez Valencia Facultad de Ciencias Químicas e Ingeniería Universidad Autónoma del Estado de Morelos Cuernavaca México Laboratorio de Espectroscopia Instituto de Ciencias Físicas Universidad Nacional Autónoma de México Cuernavaca México Instituto de Ciencias Nucleares Universidad Nacional Autónoma de México México City México Centro de Investigación en Ingeniería y Ciencias Aplicadas UAEM Cuernavaca México

For a long time and until now, rubber is the most used material for the manufacture of tires for motor vehicles. Unfortunately, once the tire meets its life cycle, the remaining rubber cannot be recycled, so the tires are discarded in collection centers and often in clandestine dumps. This represents a serious environmental problem because, in one case, these waste tires become breeding grounds for insects and wildlife that is harmful to humans. In the second case, the tires are burned, releasing highly damaging gases into the atmosphere. On the other hand, concrete is worldwide the construction material par excellence. It is basically composed of cement, gravel and sand. Mixing these three components in different proportions, their mechanical strength in compression can be increased. However, due to its fragile nature, concrete, once a crack is formed, it rapidly advances by fragmenting the material and producing its rapid collapse. In the present work, in order contribute to the care of the environment as well as to modify the fracture mode of the concrete, rubber particles obtained from waste tires were used as sand substitute in hydraulic concrete. In addition, rubber modified samples concrete were lately exposed to 70 kGy of gamma radiation in order to study the effects of this radiation on the mechanical deformation of concrete. The results showed a decrease in the mechanical properties of the concrete with rubber particles with respect to the traditional concrete itself. However, such decreases were offset by the fact that samples with rubber addition do not collapses as fast as the free rubber samples. The acquired data pave the way for research with great benefits, such as the use of recycled tires in concrete for its fracture mode modification in a beneficial way, as well as a possible decrease in the cost of concrete.

关键词： Hydraulic Concrete Scrap Tire Rubber Strength Gamma Radiation Mechanical Properties

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Description of the Health Status of Colombian Indigenous Communities Through Basic Laboratory Tests

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Journal of Life Sciences 2014年第9期8卷 789-793页

作者： Martha castillo Ana Lucia Oliveros Ana isabel Mora Faculty of Health Sciences Universidad Colegio Mayor de Cundinamarca Calle 28 No. 5B-02 Bogot6 D.C. 110911 Colombia

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Association of Thalassemia and Trait, Sickle Cell and Trait and Haemoglobin C with Iron-Deficiency Anemia in Colombians of African Descent

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Journal of Life Sciences 2014年第11期8卷 861-864页

作者： Martha castillo Ana Lucia Oliveros Ana isabel Mora Bautista Faculty of Health Sciences Universidad Colegio Mayor de Cundinamarca Calle 28 No. 5B-02 Bogot6 D.C. 110911 Colombia

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Dendritic cell deficiencies persist seven months after SARS-CoV-2 infection

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Cellular & Molecular Immunology 2021年第9期18卷 2128-2139页

作者： Alberto Pérez-Gómez Joana Vitallé Carmen Gasca-Capote Alicia Gutierrez-Valencia María Trujillo-Rodriguez Ana Serna-Gallego Esperanza Muñoz-Muela María de los Reyes Jiménez-Leon Mohamed Rafii-El-Idrissi Benhnia Inmaculada Rivas-Jeremias Cesar Sotomayor Cristina Roca-Oporto Nuria Espinosa Carmen Infante-Domínguez Juan Carlos Crespo-Rivas Alberto Fernández-Villar Alexandre Pérez-González Luis Fernando López-Cortés Eva Poveda Ezequiel Ruiz-Mateos JoséMiguel Cisneros Sonsoles Salto-Alejandre Judith Berastegui-Cabrera Pedro Camacho-Martínez Carmen Infante-Domínguez Marta Carretero-Ledesma Juan Carlos Crespo-Rivas Eduardo Márquez JoséManuel Lomas Claudio Bueno Rosario Amaya JoséAntonio Lepe Jerónimo Pachón Elisa Cordero Javier Sánchez-Céspedes Manuela Aguilar-Guisado Almudena Aguilera Clara Aguilera Teresa Aldabo-Pallas Verónica Alfaro-Lara Cristina Amodeo Javier Ampuero María Dolores Avilés Maribel Asensio Bosco Barón-Franco Lydia Barrera-Pulido Rafael Bellido-Alba Máximo Bernabeu-Wittel Candela Caballero-Eraso Macarena Cabrera Enrique Calderón Jesús Carbajal-Guerrero Manuela Cid-Cumplido Yael Corcia-Palomo Juan delgado Antonio Domínguez-Petit Alejandro Deniz Reginal Dusseck-Brutus Ana Escoresca-Ortega Fátima Espinosa Nuria Espinosa Michelle Espinoza Carmen Ferrándiz-Millón Marta Ferrer Teresa Ferrer Ignacio Gallego-Texeira Rosa Gámez-Mancera Emilio García Horacio García-delgado Manuel García-Gutiérrez María Luisa Gascón-castillo Aurora González-Estrada Demetrio González Carmen Gómez-González Rocío González-León Carmen Grande-Cabrerizo Sonia Gutiérrez Carlos Hernández-Quiles Inmaculada Concepción Herrera-Melero Marta Herrero-Romero Luis Jara Carlos Jiménez-Juan Silvia Jiménez-Jorge Mercedes Jiménez-Sánchez Julia Lanseros-Tenllado Carmina López isabel López Álvaro López-Barrios Luis F.López-Cortés Rafael Luque-Márquez Daniel Macías-García Guillermo Martín-Gutiérrez Luis Martín-Villén JoséMolina Aurora Morillo María Dolores Navarro-Amuedo Dolores Nieto-Martín Francisco Ortega María Paniagua-García Amelia Peña-Rodríguez E Clinical Unit of Infectious Diseases Microbiology and Preventive MedicineInstitute of Biomedicine of Seville(IBiS)Virgen del Rocío University HospitalCSICUniversity of SevilleSevilleSpain Department of Medical Biochemistry Molecular Biologyand ImmunologySchool of MedicineUniversity of SevilleSevilleSpain Pneumology Service Galicia Sur Health Research Instituto(IIS Galicia Sur)SERGAS-UVigoVigoSpain Infectious Diseases Unit Department of Internal MedicineComplexo HospitalarioUniversitario de VigoIIS Galicia SurSERGAS-UVigoVigoSpain Group of Virology and Pathogenesis Galicia Sur Health Research Institute(IIS Galicia Sur)-Complexo Hospitalario Universitario de VigoSERGAS-UVigoVigoSpain Unit of Infectious Diseases Microbiologyand Preventive MedicineVirgen del Rocío University HospitalSevilleSpain Institute of Biomedicine of Seville(IBiS) Virgen del Rocío University Hospital/CSIC/University of SevilleSevilleSpain Medico-Surgical Unit of Respiratory Diseases Virgen del Rocío University HospitalSevilleSpain Unit of Emergencies Virgen del Rocío University HospitalSevilleSpain Intensive Care Unit Virgen del Rocío University HospitalSevilleSpain Department of Medicine University of SevilleSevilleSpain Cardiology and Cardiovascular Surgery Unit Virgen del Rocío University HospitalSevilleSpain Rheumatology Unit Virgen del Rocío University HospitalSevilleSpain Internal Medicine Unit Virgen del Rocío University HospitalSevilleSpain Digestive and Hepatobiliary Unit Virgen del Rocío University HospitalSevilleSpain Neurology and Clinical Neurophysiology Unit Virgen del Rocío University HospitalSevilleSpain Endocrinology and Nutrition Unit Virgen del Rocío University HospitalSevilleSpain Urology and Nephrology Unit Virgen del Rocío University HospitalSevilleSpain Unit of Clinical Investigation and Clinical Trials Virgen del Rocío University HospitalSevilleSpain

Severe Acute Respiratory Syndrome Coronavirus(SARS-CoV)-2 infection induces an exacerbated inflammation driven by innate immunity *** cells(DCs)play a key role in the defense against viral infections,for instance plasmacytoid DCs(pDCs),have the capacity to produce vast amounts of interferon-alpha(IFN-α).In COVID-19 there is a deficit in DC numbers and IFN-αproduction,which has been associated with disease *** this work,we described that in addition to the DC deficiency,several DC activation and homing markers were altered in acute COVID-19 patients,which were associated with multiple inflammatory ***,previously hospitalized and nonhospitalized patients remained with decreased numbers of CD1c+myeloid DCs and pDCs seven months after SARS-CoV-2 ***,the expression of DC markers such as CD86 and CD4 were only restored in previously nonhospitalized patients,while no restoration of integrinβ7 and indoleamine 2,3-dyoxigenase(IDO)levels were *** findings contribute to a better understanding of the immunological sequelae of COVID-19.

关键词： SARS-CoV-2 COVID-19 Dendritic cell Long-COVID

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