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Studies on Some Plant Extracts for Their Antimicrobial Potential against Certain Pathogenic Microorganisms

American Journal of Plant Sciences 2012年第2期3卷 209-213页

作者： Muzafar Sheikh Abdul Rashid Malik M. K. Meghavanshi irshad mahmood Department of Botany Bundelkhand University Jhansi India. Plant Pathology Lab Department of Botany Aligarh Muslim University Aligarh India

The aim of the present study was to check the antimicrobial potential of eleven different aqueous leaf extracts on Xanthomonas campestris, Agrobacterium rhizogenes and Aspergillus fumigatus based on formation of the zone of inhibition (ZOI). Prosopis juliflora showed maximum and significant inhibitory effect on the growth of all the three pathogens. In case of Xanthomonas campestris the effect of this plant extract was almost equal to the strength of Streptomycin 10 mcg, (the metric system uses the term mcg to represent micrograms). Aqueous extracts of some plants formed low zone of inhibition. While some plant extracts were comparatively more effective against all the three pathogens (Xanthomonas campestris, Agrobacterium rhizogenes and Aspergillus fumigatus).

关键词： Zone of Inhibition Aqueous Leaf Extracts Antimicrobial Activity

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Studies on Some Plant Extracts for Their Antimicrobial Potential against Certain Pathogenic Microorganisms

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American Journal of Plant Sciences（美国植物学期刊） 2012年第2期

作者： Muzafar Sheikh Abdul Rashid Malik M. K. Meghavanshi irshad mahmood

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Characterization of purified β-glucosidase produced from Trichoderma viridethrough bio-processing of orange peel waste

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Advances in Bioscience and Biotechnology 2013年第10期4卷 941-944页

作者： Muhammad irshad Zahid Anwar Muhammad Ramzan Zahed mahmood Haq Nawaz Department of Biochemistry NSMC University of Gujrat Gujrat Pakistan Department of Chemistry and Biochemistry University of Agriculture Faisalabad Pakistan Institute of Animal Nutrition & Feed Technology University of Agriculture Faisalabad Pakistan

In the present study, solid state fermentation was carried out using orange peel waste to produce β-glucosidase from Trichoderma viride. A locally isolated fungal strain T. viride was cultured in the solid state medium of orange peel (50% w/w moisture) under optimized fermentation conditions and maximum activity of 515 ± 12.4 U/mL was recorded after 4th day of incubation at pH 5.5 and 30℃. Indigenously produced β-glucosidase was subjected to the ammonium sulfate precipitation and Sephadex-G-100 gel filtration chromatography. In comparison to the crude extract β-glucosidase was 5.1-fold purified with specific activity of 758 U/mg. The enzyme was shown to have a relative molecular weight of 62 kDa as evidenced by sodium dodecyl sulphate polyacrylamide gel electrophoresis. The purified β-glucosidase displayed 6 and 60℃ as an optimum pH and temperature respectively.

关键词： Orange Peel Waste β-Glucosidase T. viride Purification SDS-PAGE Characterization

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Evaluation of Various Mango Varieties against the Infection Dynamics of Powdery Mildew (Oidium mangiferaeBert.)

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American Journal of Plant Sciences 2014年第15期5卷 2372-2377页

作者： Syed Atif Hasan Naqvi Rashida Perveen Syed Amir Manzoor Hafiz Muhammad Imran Umar Muhammad Tayyab Iqbal Fatima Liaquat Taha Majid Ahsan irshad Department of Forestry FAST Bahauddin Zakariya University Multan Pakistan Department of Plant Breeding & Genetics FAST Bahauddin Zakariya University Multan Pakistan Department of Plant Pathology FAST Bahauddin Zakariya University Multan Pakistan Department of Soil Science FAST Bahauddin Zakariya University Multan Pakistan

Mango (Mangifera indica L: Anacardiaceae) is regarded as the most liked fruit in tropical and sub-tropical regions of the world. Powdery mildew of mango caused by Oidium mangiferae Bert. is one of the major plant pathological constraints in growing healthy mango orchards. The apparent symptoms of the disease occurred on young tissues of all parts of flowers, leaves and fruits. Severe blossom infection could result in complete damage to fruit;flower failed to open and drop off from the inflorescence. The inflorescence revealed a pattern of disease from tip to downward and showed itself by the emergence of wefts of white mycelium on the affected parts. Twenty-five mango varieties i.e. Langra, Dusehri, Ratole No.12, Fajri, Sindhri, Chaunsa Samar Bahisht, Anwar ratole, Neelam, Yakta, Tota Pari, Sensation, Saroli, Malda, Ghulab e Khas, Chaunsa Black, Chaunsa white, Anmol, Almas, Shan e Ali, Shan e Mustafa, mahmood Khan, Armughan, Zafaran, Malda Late and Early Gold were evaluated through the observation of symptoms on young inflorescence to determine the disease incidence, disease severity index and average yield of fruit. The study was carried out in a randomized compete block design with twenty-five treatments and three replications. The mango varieties presented different performance in relation to powdery mildew incidence and could be categorized into eight groups. Maximum disease incidence was observed (33.33%, 26.66% and 26.66%, 26.66%) on Dusehri, Chaunsa Samar Bahisht, Malda and Ratole No.12 respectively and minimum disease incidence was (3.66% and 3.66%) noted on Almas and Sensation. These two varieties showed to be tolerant against the disease. It might be concluded that the presence or absence of symptoms caused by the powdery mildew had no such effect on the fruit yield of the mango cultivars.

关键词： Powdery Mildew Oidium mangiferae Bert. Infection Varieties RCBD

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