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Production of chitosan-based composite film reinforced with lignin-rich lignocellulose nanofibers from rice husk

Journal of Bioresources and Bioproducts 2024年第2期9卷 174-184页

作者： Hye Jee Kang Yeon Ju Lee Jin Kyoung Lee irnia nurika Sri Suhartini Deokyeong Choe Dong Hyun Kim Hoon Choi Natasha P.Murphy Ho Yong Kim Young Hoon Jung School of Food Science and Biotechnology Kyungpook National UniversityDaegu 41566Republic of Korea CPR S&T Co. Ltd.Gunpo 15880Republic of Korea Department of Agroindustrial Technology Faculty of Agricultural TechnologyUniversitas BrawijayaMalang 65145Indonesia Renewable Resources and Enabling Sciences Center National Renewable Energy LaboratoryGoldenCO 80401United States Center for Bio-based Chemistry Korea Research Institute of Chemical TechnologyUlsan 44429Republic of Korea

Lignocellulosic nanofibers(LCNFs),implying lignin-containing cellulose fibers,maintain the prop-erties of both lignin and cellulose,which are hydrophobic and hydrophilic,*** pres-ence of hydrophobic lignin in LCNFs is expected to be an economical and attractive option that can improve the thermal and mechanical properties of ***,this study was conducted to produce lignin-rich LCNFs from sugar-rich waste obtained from rice husks after acidic *** LCNFs were produced from the lignin-rich solid fractions obtained after pretreatment and enzymatic hydrolysis,which were then incorporated as an additive into a chitosan-based *** variations in lignin content in the range of approximately 50.6%-66.8%in differently obtained LCNFs gave significantly different optical strengths and mechanical *** controllable processes may allow for customized film ***,the glucose-rich liquid fractions obtained after pretreatment and enzymatic hydrolysis were used as a substrate for ethanol fermentation to achieve total utilization of rice husk biomass *** conclusion,the lignin-rich biomass fraction holds promise as a suitable material for chitosan-LCNF film and has the potential to increase the economic feasibility of the biomaterial industry.

关键词： Rice husk Lignocellulose nanofiber Lignin-based material Chitosan film Upcycling

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Immunogenic and Protective Properties of the First Kazakhstan Vaccine against Pandemic Influenza А(Н1N1) pdm09 in Ferrets

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Virologica Sinica 2012年第6期27卷 345-352页

作者： Kaissar Tabynov Zhailaubai Kydyrbayev Abylai Sansyzbay Berik Khairullin Sholpan Ryskeldinova nurika Assanzhanova Yerken Kozhamkulov Dulat Inkarbekov Research Institute for Biological Safety Problems (RIBSP) Gvardeiski 080409 Kazakhstan

This paper presents the results of a pre-clinical study of the immunogenicity and efficacy of an egg-derived, inactivated, whole-virion adjuvanted vaccine （Refluvac） on ferret models. For this purpose, groups of eight ferrets （6 to 7 months old） were injected with 0.5 mL of vaccine specimens containing 3.75, 7.5 or 15.0 μg of virus hemagglutinin. Administration was intramuscular and given either as a single dose or as two doses 14 days apart. All vaccine specimens manifested immunogenicity in ferrets for single （HI titer, from 51 ± 7 to 160 ± 23） and double （HI titer, from 697± 120 to 829 ± 117） administrations. To assess the protective effects of the vaccine, ferrets from the vaccinated and control groups were infected intranasally with pandemic virus A/California/7/09 （H1N1） pdm09 at a dose of 106 106/0.5 mL. Fourteen days post-infection, the ferrets inoculated with single or double vaccines containing 3.75, 7.5 or 15.0 ~g of hemagglutinin per dose showed no signs of influenza infection, weight loss, or body temperature rise, and no premature deaths occurred. The number of vaccinated ferrets shedding the virus via the upper airway, as well as the amount of virus shed after infection, was significantly reduced in comparison with animals from the control group. Based on our results, we suggest that a single vaccination at a dose of 3.75 or 7.5 μg hemagglutinin be used for Phase I clinical trials.

关键词： Vaccine Pandemic Influenza A (H1N1) pdm09 Immunogenic and Protective Properties Ferrets

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A new can didate vaccine for human brucellosis based on influenza viral vectors:a preliminary investigation for the development of an immunization schedule in a guinea pig model

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Infectious Diseases of Poverty 2021年第1期10卷 56-65页

作者： Dina Bugybayeva Zhailaubay Kydyrbayev Nadezhda Zinina nurika Assanzhanova Bolat Yespembetov Yerken Kozhamkulov Kunsulu Zakarya Sholpan Ryskeldinova Kaissar Tabynov Research Institute for Biological Safety Problems 15 MomushulyGvardeyskiy 080409Kazakhstan Kazakh National Agrarian University 8 Abay AvenueAlmaty 050010Kazakhstan Research Institute of Cardiology and Internal Medicine 120 Aiteke biAlmaty 050000Kazakhstan.

Background:A new candidate vector vaccine against human brucellosis based on recombinant influenza viral vectors(rIVV)subtypes H5N1 expressing Brucella outer membrane protein(Omp)16,L7/L12,Omp19or Cu-Zn SOD proteins has been *** paper presents the results of the study of protection of the vaccine using on guinea pigs,including various options of administering,dose and *** data of the novel vaccine candidate will contribute to its further movement into the preclinical stage ***:General states of guinea pigs was assessed based on behavior and dynamics of a guinea pig weight-gain *** effectiveness of the new anti-brucellosis vector vaccine was determined by studying its protective effect after conjunctival,intranasal and sublingual administration in doses 10^(5) EID50,10^(6) EID_(50) and 10^(7) EID_(50) during prime and boost vaccinations of animals,followed by challenge with a virulent strain of *** 16 M *** sake of comparison,the commercial ft melitensis Rev.1 vaccine was used as a *** protective properties of vaccines were assessed by quantitation of Brucella colonization in organs and tissues of infected animals and compared to the control ***:It was observed a gradual increase in body weight of guinea pigs after prime and booster immunization with the vacci ne using conjunctival,intra nasal and subli ngual routes of administration,as well as after using various doses of *** most optimal way of using the vaccine has been established:double intranasal immunization of guinea pigs at a dose of 10^(6) EID50, which provides 80%protection of guinea pigs from *** 16 M infection(P<0.05),which is comparable to the results of the effectiv en ess of the commercial *** Rev.1 vacci ***:We developed effective human vaccine candidate against brucellosis and developed its immunization protocol in guinea pig *** believe that because of these studies,the proposed

关键词： Human brucellosis Influenza viral vectors Vaccine candidate Protection Guinea pigs Immunization route Vaccination dose

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