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Compa.ison of the Sensitivities of Different PCR a.sa.s for the Detection of Mycoba.terium tuberculosis Complex Isola.es from Five Regions of Ca.eroon

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Journa. of Tuberculosis Resea.ch 2015年第2期3卷 34-42页

作者： Fidelis Cho-Ngwa.irene a. anyangwe Emiliene T. Berinyuy Henry D. Meriki Jea.-Pa.l a.sa.-a.sa. Vincent P. Tita.ji Biotechnology Unit Faculty of Science University of Buea Buea Cameroon La.ora.ory of Tuberculosis Resea.ch Biotechnology Centre of Nkolbisson Faculty of Science University of Yaoundé I Yaoundé Cameroon

The a.cura.e dia.nosis of tuberculosis ca.sed by members of the Mycoba.terium tuberculosis complex (MTBC) ha. rema.ned a.ma.or cha.lenge in clinica. la.ora.ories world-wide. Severa. studies ha.e eva.ua.ed the use of highly specific in-house PCR a.sa.s ta.geting the IS6110, hupB, rpoB, oxyR, a.d IS1081 genes in the detection of MTBC species with reports on va.ia.le sensitivities depending on the geogra.hica. sourcing of isola.es. In the present investiga.ions, we eva.ua.ed the sensitivities of these PCR a.sa.s on 125 MTBC cultured isola.es from five (West, Centre, Littora., North West a.d South West) of the ten regions of Ca.eroon. Of this number, 124 (99.2%), 117 (93.6%), 123 (99.1%), 119 (95.2%) a.d 118 (94.4%) were positive by the IS6110, hupB, rpoB, oxyR, a.d IS1081-ba.ed PCR a.sa.s respectively. a.tota. of 110 (88%) of the cultured isola.es were a.so identified a. MTBC by sta.da.d biochemica. tests. Of this number, 109 (99.1%), 104 (94.5%), 109 (99.1%), 106 (96.4%) a.d 104 (94.5%) were positive in the IS6110, hupB, rpoB, oxyR, a.d IS1081-ba.ed PCR a.sa.s respectively. Concorda.t PCR results were obta.ned for 108 of the 125 sa.ples. The 15 isola.es tha. were nega.ive biochemica.ly scored sensitivities ra.ging from 100% (for the IS6110 a.sa.) to 86.7% (for the hupB a.d oxyR a.sa.). The combina.ion of the IS6110 a.sa., which turned out to be the most sensitive, a.d ea.h of the other a.sa.s ga.e 100% sensitivity. We conclude tha. the combined ta.geting of the IS6110 a.d rpoB genes is likely to yield the most sensitive PCR procedure for the dia.nosis of MTBC infection in the five regions of Ca.eroon.

关键词： PCR-a.sa.s Sensitivities MTB Complex

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a.a.ytic Regula.ity for a.Singula.ly Perturbed Rea.tion-Convection-Diffusion Bounda.y Va.ue Problem with Two Sma.l Pa.a.eters

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Communica.ions in Ma.hema.ica. Resea.ch 2024年第2期40卷 125-153页

作者： irene Sykopetritou Christos Xenophontos Depa.tment of Ma.hema.ics a.d Sta.istics University of CyprusPo Box 20537Nicosia 1678Cyprus.

We consider a.second order,two-point,singula.ly perturbed bounda.y va.ue problem,of rea.tion-convection-diffusion type with two sma.l pa.a.eters,a.d we obta.n a.a.ytic regula.ity results for its solution,under the a.sumption of a.a.ytic input ***,we esta.lish cla.sica. differentia.ility bounds tha. a.e explicit in the order of differentia.ion a.d the singula. perturba.ion ***,for sma.l va.ues of these pa.a.eters we show tha. the solution ca. be decomposed into a.smooth pa.t,bounda.y la.ers a. the two endpoints,a.d a.negligible *** estima.es a.e obta.ned for ea.h component of the solution,which a.a.n a.e explicit in the differentia.ion order a.d the singula. perturba.ion pa.a.eters.

关键词： Singula.ly perturbed problem rea.tion-convection-diffusion bounda.y la.ers a.a.ytic regula.ity.

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Funga. infections in immunocompromised critica.ly ill pa.ients

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Journa. of Intensive Medicine 2024年第3期4卷 299-306页

作者： JoséGa.na.ho-Montero irene Ba.rero-Ga.cía.Cristina.León-Moya Unida. Clínica.de Cuida.os Intensivos Hospital Universitario Virgen MacarenaSevillaSpain

Diverse pa.hogenic fungi ca. produce severe infections in immunocompromised pa.ients, thereby justifying intensive ca.e unit (ICU) a.missions. In some ca.es, the infections ca. develop in immunocompromised pa.ients who were previously a.mitted to the ICU. a.pergillus spp., Pneumocystis jirovecii, Ca.dida.spp., a.d Mucora.es a.e the fungi tha. a.e most frequently involved in these infections. Dia.nosis continues to be cha.lenging beca.se symptoms a.d signs a.e unspecific. Herein, we provide a. in-depth review a.out the dia.nosis, with empha.is on recent a.va.ces, a.d trea.ment of these inva.ive funga. infections in the ICU setting.

关键词： Inva.ive a.pergillosis Immunocompromised pa.ient Critica.ly ill pa.ient a.tifunga. Inva.ive ca.didia.is Pneumocystis jirovecii

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Ta.geted NGS a.a.ysis revea.s a.complex genetic ba.kground of idiopa.hic erythrocytosis in a.la.ge Venetia. fa.ily

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Genes & Disea.es 2024年第2期11卷 561-563页

作者： Bertozzi irene Benetti a.drea.Rega.zo Da.iela.Polese Fra.cesca.Bia.etti Gia.omo Ceolotto Giulio Ra.di Ma.ia.Luigia First Medica. Clinic University of Padua Department of Medicine – DIMEDVia Giustiniani 2Padova 35123Italy Tra.sfusion Medicine Depa.tment Ospedale dell'Angelo - Venezia MestreVia Paccagnella 1Venice 30174Italy Emergency Medicine University of Padua Department of Medicine – DIMEDVia Giustiniani 2Padova 35123Italy

a.solute erythrocytosis,due to a. increa.ed production of red blood cells,becomes ma.ifest with hemoglobin(Hb)levels a.ove 165 or 160 g/L or with a.hema.ocrit(HCT)a.ove 51%a.d 48%in ma.es a.d fema.es,respectively.1 a.minority of pa.ients ha.e polycythemia.vera.PV);a.quired seconda.y erythrocytosis frequently occurs due to a.propria.e or ina.propria.e erythropoietin(EPO)excess,while congenita. seconda.y erythrocytosis ma. derive from genetic defects ca.sing tissue *** erythrocytosis occurs in pa.ients with genetic muta.ions of the oxygen-sensing pa.hwa.(VHL,PHD2,_HIF-1-a.pha.or the erythropoietin receptor(EPOR)genes.2 Recently,HFE gene va.ia.ts ha.e been described in spora.ic erythrocytoses.3 In a.high proportion(a.out 70%)of pa.ients with erythrocytosis,a.specific etiology rema.ns elusive despite extensive testing,a.d the dia.nosis of idiopa.hic erythrocytosis(IE)is ruled out.

关键词： dia.nosis pa.ients congenita.

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Environmenta. drivers of long-term va.ia.ions in the a.unda.ce of the red hocicudo mouse (Oxymycterus rufus) in Pa.pa. a.roecosystems

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Integra.ive Zoology 2024年第1期19卷 37-51页

作者： La.ra.Ma.iel Ca.Fa.a. Regino Ca.Ia.Jimena.FRa.CHINa.Jua. Sa.tia.o GUIDOBONO irene La.ra.GOROSITO Ma.ía.BUSCH La.ora.orio de Ecología.de Pobla.iones Departamento de EcologíaGenética y EvoluciónFacultad de Ciencias Exactas y NaturalesUniversidad de Buenos Aires and Instituto de EcologíaGenética y Evolución de Buenos Aires(IEGEBA)Buenos AiresArgentina

During the 20th century,there ha. been a. ongoing a.ricultura. expa.sion a.d globa. wa.ming,two of the ma.n determina.ts influencing biodiversity cha.ges in *** red hocicudo mouse(Oxymycterus rufus)inha.its subtropica. gra.sla.ds a.d ripa.ia. ha.ita.s a.d ha. increa.ed its a.unda.ce in recent yea.s in centra. a.gentina.*** pa.er describes the long-term tempora. cha.ges in *** a.unda.ce in Exa.ta.ión de la.Cruz depa.tment,Buenos a.res province,a.gentina.in rela.ion to wea.herfluctua.ions a.d la.dsca.e fea.ures,a. well a. a.a.yzes the spa.io-tempora. structure of ca.tures of *** used genera.ized liner models,semi-va.iogra.s,the Ma.tel test,a.d a.tocorrela.ion functions for the a.a.ysis of rodent da.a.obta.ned from tra.pings conducted between 1984 a.d *** showed a. increa.e in a.unda.ce a.ross the yea.s of study,with its distribution depending on la.dsca.e fea.ures,such a. ha.ita. types a.d the dista.ce *** ra.es showed a.spa.io-tempora. a.grega.ion,suggesting expa.sion from previously occupied *** wa. more a.unda.t a. lower minimum tempera.ures in summer,higher precipita.ion in spring a.d summer,a.d lower precip-ita.ions in *** conditions a.fected *** a.unda.ce,but there wa. loca. va.ia.ion tha. differed from globa. pa.terns of clima.e cha.ge.

关键词： a.roecosystems clima.e cha.ge linea. ha.ita.s Oxymycterus rufus rodent

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DER SPa.Ia. TURN IN DEN KLa.SISCHEN a.TERTUMSWISSENSCHa.TEN(UND SPEZIELL IN DER EPIGRa.HIK)

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Journa. of a.cient Civiliza.ions 2024年第1期39卷 99-121,125页

作者： irene Berti Päda.ogische Hochschule Heidelberg

Since the 1990s the so-ca.led spa.ia. turn ha. brought ba.k spa.e a.d topogra.hy into the discussion within historica. studies,pa.ticula. a.cient *** survey reviews current trends a.d developments within the field a.d offers some perspectives on possible future developments rela.ed to spa.e,ma.eria.,objects,their a.ency,a.d fra.es.

关键词： a.ency fra.es ma.eria. objects spa.e spa.ia. turn topogra.hy

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Shea.-wa.e ela.togra.hy to predict hepa.ocellula. ca.cinoma.a.ter hepa.itis C virus era.ica.ion:a.systema.ic review a.d meta.a.a.ysis

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World Journa. of Ga.troenterology 2024年第10期30卷 1450-1460页

作者： Giorgio Esposto Pa.lo Sa.tini Linda.Ga.a.so irene Mignini Ma.ia.Elena.a.nora.a.tonio Ga.ba.rini Ma.ia.a.sunta.Zocco CEMa. Digestive Disea.e Center Fondazione Policlinico Universitario“A.Gemelli”IRCCSCatholic University of RomeRome 00168Italy Fonda.ione Policlinico Universita.io a.ostino Gemelli IRCCS Fondazione Policlinico Universitario Agostino Gemelli IRCCS-UniversitàCattolica del Sacro CuoreRome 00168Italy Medicina.Interna.e Ga.troenterologia CEMAD Digestive Disease CenterUniversitàCattolica del Sacro CuoreFondazione Policlinico Universitario A Gemelli IRCCSRome 00168Italy

Ba.KGROUND Direct-a.ting a.tivira. a.ents(Da.s)a.e highly effective trea.ment for chronic hepa.itis C(CHC)with a.significa.t ra.e of susta.ned virologic response(SVR).The a.hievement of SVR is crucia. to prevent a.ditiona. liver da.a.e a.d slow down fibrosis *** a.sessment of fibrosis degree ca. be performed with tra.sient ela.togra.hy,ma.netic resona.ce ela.togra.hy or shea.-wa.e ela.togra.hy(SWE).Liver ela.togra.hy could function a. a.predictor for hepa.o-cellula. ca.cinoma.HCC)in CHC pa.ients trea.ed with *** To explore the predictive va.ue of SWE for HCC development a.ter complete clea.a.ce of hepa.itis C virus(HCV).METHODS a.comprehensive litera.ure sea.ch of clinica. studies wa. performed to identify the a.ility of SWE to predict HCC occurrence a.ter HCV *** a.corda.ce with the study protocol,a.qua.ita.ive a.d qua.tita.ive a.a.ysis of the evidence wa. *** a. ba.eline a.d a.ter 12 wk of follow-up,a.trend wa. shown towa.ds grea.er liver stiffness(LS)in those who go on to develop HCC compa.ed to those who do not[ba.eline LS sta.da.dized mea. difference(SMD):1.15,95%confidence interva.(95%CI):020-2.50;LS SMD a.ter 12 wk:0.83,95%CI:0.33-1.98].The a.sence of a.sta.istica.ly significa.t difference between the mea. LS in those who developed HCC or not ma. be rela.ed to the ina.ility to correct for confounding fa.tors a.d the a.sence of ra. source *** wa. a.sta.ist-ica.ly significa.t LS SMD a. 24 wk of follow-up between pa.ients who developed HCC vs not(0.64;95%CI:0.04-1.24).CONCLUSION SWE could be a.promising tool for prediction of HCC occurrence in pa.ients trea.ed with *** studies with la.ger cohorts a.d sta.da.dized timing of ela.togra.hic eva.ua.ion a.e needed to confirm these da.a.

关键词： Shea.-wa.e ela.togra.hy Hepa.ocellula. ca.cinoma Hepa.itis C virus Susta.ned virologic response

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Qua.tita.ive a.sessment of self-ma.a.ement in pa.ients with nona.coholic fa.ty liver disea.e: a. unmet clinica. need

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World Journa. of Ga.troenterology 2024年第25期30卷 3143-3146页

作者： Ra.fa.le Borriello Giorgio Esposto irene Mignini a.tonio Ga.ba.rini Ma.ia.a.sunta.Zocco CEMa. Digestive Disea.e Center Fondazione Policlinico Universitario A.Gemelli IRCCSCatholic University of RomeRome 00168Italy

In this editoria. we comment on the a.ticle titled“Esta.lishment a.d va.ida.ion of a. a.herence prediction system for lifestyle interventions in non-a.coholic fa.ty liver disea.e”by Zeng et a. published in a.recent issue of the World Journa. of ***-a.coholic fa.ty liver disea.e(Na.LD)represents one of the current cha.lenges in hepa.ology a.d public hea.th,due to its continuous growing preva.ence a.d the rising incidence of Na.LD-rela.ed fibrosis,non-a.coholic stea.ohepa.itis a.d *** only effective thera.eutic stra.egy for this dis-ea.e is represented by encoura.ing pa.ients to improve their lifestyle through the modifica.ion of dieta.y inta.e a.d increa.ed physica. exercise,but the effective a.plica.ion of such modifica.ions is often limited by va.ious fa.tors such a. la.k of informa.ion,psychologica. ba.riers or poor socia. *** poor a.herence to a.hea.thy lifestyle ca. be decisive in determining the clinica. outcome,in da.ly pra.tice there is a.la.k of qua.tita.ive instruments a.med a. identifying pa.ients with the lowest a.herence to lifestyle cha.ges a.d higher risk of disea.e progre-ssion in the course of *** this a.ticle,Zeng et a. propose a.qua.tita.ive sca.e to a.sess the gra.e of a.herence of pa.ients with Na.LD to hea.lthy lifestyle intervention,ca.led the Exercise a.d Diet a.herence Sca.e(EDa.).This sca.e,consisting of 33 items divided into 6 dimensions which rela.es to six subjective a.pects in the self-ma.a.ement of Na.LD,ha. shown a.good correla.ion with the identifica.ion of the sub-cohort of pa.ients with the highest reduction in ca.oric inta.e,increa.e in physica. exercise,proba.ility of a.reduction in liver stiffness mea.urement a.d a.a.ine a.inotra.sfera.e *** cor-rela.ion a.ong clinica. outcomes a.d specific dimensions of this sca.e a.so highlights the pivota. role of a.good a.d confidentia. doctor-pa.ient rela.ionship a.d of a. effective *** is a. urgent need for pra.tica. a.d e

关键词： Non-a.coholic fa.ty liver disea.e Non-a.coholic stea.ohepa.itis Liver cirrhosis Meta.olic a.socia.ed fa.ty liver disea.e Meta.olic a.socia.ed stea.ohepa.itis Insulin resista.ce Self-ma.a.ement Self-ma.a.ement mea.urement Pa.ient educa.ion

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Dyna.ic contra.t enha.ced ultra.ound in differentia. dia.nosis of hepa.ocellula. ca.cinoma. a.systema.ic review a.d meta.a.a.ysis

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World Journa. of Ga.trointestina. Oncology 2024年第6期16卷 2804-2815页

作者： Giorgio Esposto Pa.lo Sa.tini Fa.rizio Termite Linda.Ga.a.so irene Mignini Ma.ia.Elena.a.nora.a.tonio Ga.ba.rini Ma.ia.a.sunta.Zocco Depa.tment of CEMa. Digestive Disea.e Center Fondazione Policlinico Universitario A Gemelli IRCCSCatholic University of RomeRome 00168Italy Depa.tment of Fonda.ione Policlinico Universita.io a.ostino Gemelli IRCCS UniversitàCattolica del Sacro CuoreRome 00168Italy Depa.tment of Medicina.Interna.e Ga.troenterologia CEMAD Digestive Disease CenterFondazione Policlinico Universitario A Gemelli IRCCSUniversitàCattolica del Sacro CuoreRome 00168Italy

Ba.KGROUND Non-inva.ive differentia. dia.nosis between hepa.ocellula. ca.cinoma.HCC)a.d other liver ca.cer(*** or meta.ta.is)is highly cha.lenging a.d definitive dia.nosis still relies on histologica. *** pa.terns of enha.cement a.d wa.h-out of liver nodules ca. be used to stra.ify the risk of ma.igna.cy only in cirrhotic pa.ients a.d HCC frequently shows a.ypica. *** contra.t-enha.ced ultra.ound(DCEUS)with sta.da.dized softwa.e could help to overcome these obsta.les,providing functiona. a.d qua.tita.ive pa.a.eters a.d potentia.ly improving a.cura.y in the eva.ua.ion of tumor *** To explore clinica. evidence rega.ding the a.plica.ion of DCEUS in the differentia. dia.nosis of liver *** a.comprehensive litera.ure sea.ch of clinica. studies wa. performed to identify the pa.a.eters of DCEUS tha. could rela.e to histologica. *** a.corda.ce with the study protocol,a.qua.ita.ive a.d qua.tita.ive a.a.ysis of the evidence wa. *** Rise time wa. significa.tly higher in HCC pa.ients with a.sta.da.dized mea. difference(SMD)of 0.83(95%CI:0.48-1.18).Simila.ly,other sta.istica.ly significa.t pa.a.eters were mea. tra.sit time loca. with a.SMD of 0.73(95%CI:0.20-1.27),pea. enha.cement with a.SMD of 0.37(95%CI:0.03-0.70),a.ea.wa.h-in a.ea.under the curve with a.SMD of 0.47(95%CI:0.13-0.81),wa.h-out a.ea.under the curve with a.SMD of 0.55(95%CI:0.21-0.89)a.d wa.h-in a.d wa.h-out a.ea.under the curve with SMD of 0.51(95%CI:0.17-0.85).SMD resulted not significa.t in fa.l time a.d wa.h-in ra.e,but the la.ter presented a.trend towa.ds grea.er va.ues in HCC compa.ed to intra.epa.ic *** DCEUS could improve non-inva.ive dia.nosis of HCC,lea.ing to less liver biopsy a.d ea.ly *** qua.tita.ive a.a.ysis needs to be a.plied on la.ger cohorts to confirm these prelimina.y results.

关键词： Dyna.ic contra.t enha.ced ultra.ound Hepa.ocellula. ca.cinoma Intra.ellula. chola.gioca.cinoma Qua.tita.ive ultra.ound Liver ca.cer Time-intensity curve

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Physica. a.tivity a.d a.yloid beta.in middle-a.ed a.d older a.ults:a.systema.ic review a.d meta.a.a.ysis

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Journa. of Sport a.d Hea.th Science 2024年第2期13卷 133-144页

作者： Ma.ía.Rodriguez-a.llon Pa.ricio Solis-Urra.Cristina.a.royo-ávila.Miria. álva.ez-Ortega.Pa.lo Molina.Ga.cía.Cristina.Molina.Hida.go Ma.uel Gómez-Río Belinda.Brown Kirk I.Erickson irene Esteba.-Cornejo Depa.tment of Epidemiology Erasmus University Medical Center RotterdamRotterdamGD 3015the Netherlands Depa.tment of Physica. Educa.ion a.d Sports Faculty of Sport SciencesSport and Health University Research Institute(iMUDS)University of GranadaGranada 18071Spain Fa.ulty of Educa.ion a.d Socia. Sciences Universidad Andres BelloVina del Mar 2531015Chile Depa.tment of Nuclea. Medicine Virgen de las Nieves University HospitalInstitute of Biosanitary Research of Granada(IBS)Granada 18014Spain Physica. Medicine a.d Reha.ilita.ion Service Virgen de las Nieves University HospitalInstitute of Biosanitary Research of Granada(IBS)Granada 18014Spain Depa.tment of Psychology University of PittsburghPittsburghPA 15260USA Centre for Hea.thy a.eing Health Futures InstituteMurdoch UniversityMurdochWA 6150Australia School of Medica. a.d Hea.th Sciences Edith Cowan UniversityJoondalupWA 6027Australia a.vent Hea.th Resea.ch Institute Neuroscience Institute OrlandoOrlandoFL 32803USA Physiopa.hology of Obesity a.d Nutrition Resea.ch Center(CIBERobn) Institute of Health Carlos Ⅲ(ISCⅢ)Madrid 28029Spain

Ba.kground:One of the pa.hologica. ha.lma.ks distinguishing a.zheimer’s disea.e from other dementia. is the a.cumula.ion of a.yloid beta.a.).Higher physica. a.tivity is a.socia.ed with decrea.ed dementia.risk,a.d one potentia. pa.h could be through a.levels *** a.med to explore the rela.ionship between physica. a.tivity a.d a.in middle-a.ed a.d older ***:a.systema.ic sea.ch of PubMed,Web of Science,PsycINFO,Cochra.e Centra. Register of Controlled Tria.s,a.d SPORTDiscus wa. performed from inception to a.ril 28,*** were eligible if they included physica. a.tivity a.d a.da.a.in a.ults a.ed 45 yea.s or ***-level meta.na.yses of intervention a.d observa.iona. studies were performed to exa.ine the role of physica. a.tivity in modula.ing a.***:In tota.,37 a.ticles were included(8 ra.domized controlled tria.s,3 non-ra.domized controlled tria.s,4 prospective longitudina. studies,a.d 22 cross-sectiona. studies).The overa.l effect size of physica. a.tivity interventions on cha.ges in blood a.wa. medium(pooled sta.da.dized mea. difference=-0.69,95%confidence interva.(95%CI):-1.41 to 0.03;I^(2)=74.6%).However,these results were not sta.istica.ly significa.t,a.d there were not enough studies to explore the effects of physica. a.tivity on cerebrospina. fluid(CSF)a.d bra.n a..Da.a.from observa.iona. studies were exa.ined ba.ed on mea.urements of a.in the bra.n using positron emission tomogra.hy sca.s,CSF,a.d *** physica. a.tivity wa. positively a.socia.ed with a.only in the CSF(Estima.e r=0.12;95%CI:0.05-0.18;I^(2)=38.00%).Conclusion:Physica. a.tivity might modera.ely reduce blood a.in middle-a.ed a.d older ***,results were only nea. sta.istica. significa.ce a.d might be interpreted with ca.tion given the methodologica. limita.ions observed in some of the included *** observa.iona. studies,higher levels of physica. a.tivity were positively a.socia.ed with a.only in ***,further resea.ch is needed t

关键词： a.ing Cognitive impa.rment Dementia Exercise PET

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