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Color Doppler sonography and angioscintigraphy in hepatic Hodgkin's lymphoma

World Journal of Gastroenterology 2009年第26期15卷 3269-3275页

作者： Mirjana V Stojkovi Vera M Artiko irena B Radoman Slavko J Knezevi Snezana M Luki Mirko D Kerkez Nebojsa S Leki Andrija A Anti Marinko M Zuvela Vitomir I Rankovi Milorad N Petrovi Dragana P obi Vladimir B Obradovi Institute for Digestive Diseases Clinical Center of Serbia Institute for Nuclear Medicine Clinical Center of Serbia Gastroenterology Department Clinical Center Podgorica Gastroenterology Department Clinical Center Kragujevac

AIM： To estimate the characteristics of Color Doppler findings and the results of hepatic radionuclide angiography （HRA） in secondary Hodgkin＇s hepatic lymphoma. METHODS： The research included patients with a diagnosis of Hodgkin＇s lymphoma with metastatic focal lesions in the liver and controls. Morphologic characteristics of focal liver lesions and hemodynamic parameters were examined by pulsed and Color Doppler in the portal, hepatic and splenic veins were examined. Hepatic perfusion index （HPI） estimated by HRA was calculated. was observed. Lesions were mostly hypoechoic and mixed, solitary or multiple. Some of the patients presented with dilated splenic veins and hepatofugal blood flow. A pulse wave was registered in the centre and at the margins of lymphoma. The average velocity of the pulse wave was higher at the margins （P 〉 0.05）. A continuous venous wave was found only at the margins of lymphoma. There was no linear correlation between lymphoma size and velocity of pulse and continuous wave （r = 390, P 〈 0.01）. HPI was significantly lower in patients with lymphomas than in controls （P 〈 0.05）, pointing out increased arterial perfusion in comparison to portal perfusion. CONCLUSION： Color Doppler ultrasonography is a sensitive method for the detection of neovascularization in Hodgkin＇s hepatic lymphoma and estimation of its intensity. Hepatic radionuclide angiography can additionally help in the assesment of vascularisation of liver lesions.

关键词： Color Doppler ultrasonography Hodgkin's disease Lymphoma Liver Radionuclide angiography Velocity Hepatic artery Portal vein Blood flow Splenomegaly

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Leptin gene polymorphism a昀ects leptin level in childhood asthma

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World Journal of Pediatrics 2018年第6期14卷 601-606页

作者： Dawid Szczepankiewicz Paulina Sobkowiak Beata Naro(z)na irena Wojsyk-Banaszak Anna Br(e)borowicz Aleksandra Szczepankiewicz Department of Animal Physiology and Biochemistry Poznan University of Life SciencesPoznanPoland Department of Pediatric Pulmonology Allergy and Clinical ImmunologyIIIrd Department of PediatricsPoznan University of Medical SciencesPoznanPoland Laboratory of Molecular and Cell Biology Department of Pediatric PulmonologyAllergy and Clinical ImmunologyIIIrd Department of PediatricsPoznan University of Medical SciencesSzpitalna St.27/3360-572 P oznanPoland

Background Leptin may induce inflammation in asthma by activation of Th2 cells. It has also been demonstrated that leptin expression increases upon inflammation and that asthmatic patients show increased serum leptin levels. We hypothesized that the polymorphism in leptin (LEP) and leptin receptor (LEPR) genes is associated with childhood asthma and may affect their serum level. To our knowledge, there are no reports analyzing LEP and LEPR polymorphisms in association with their serum levels in childhood asthma. Methods We analyzed 35 subjects: 25 asthmatic pediatric patients and 10 healthy children aged from 6 to 18. The diagnosis of allergic asthma was based on clinical manifestation, lung function, positive skin prick tests and increased immunoglobulin E levels. The polymorphisms were genotyped with use of polymerase chain reaction-restriction fragment length polymor-phism method. Serum levels of leptin and leptin receptor were determined using BioVendor enzyme-linked immunosorbent assay kits. Statistical analysis was done with Statistica v.12. Results We observed that leptin levels were increased in asthmatic subjects as compared to healthy controls and were sig-nificantly higher during exacerbation than in the asymptomatic period (P=0.025). We observed that LEP polymorphism (rs13228377) was associated with higher serum leptin levels in asthma and these two variables had high predictive value for asthma risk (P=0.007, odds ratio 17.5, predictive accuracy 83.9%). LEPR polymorphisms did not show association with its serum level and asthma risk. Conclusion LEP polymorphism may increase asthma risk via influence on its serum level.

关键词： Asthma Gene Leptin Leptin receptor Polymorphism

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Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections

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World Journal of Methodology 2021年第6期11卷 294-301页

作者： Tatjana Vilibic-Cavlek Ljubo Barbic Vladimir Stevanovic Vladimir Savic Anna Mrzljak Maja Bogdanic irena Tabain School of Medicine University of ZagrebZagreb 10000Croatia Department of Virology Croatian Institute of Public HealthZagreb 10000Croatia Department of Microbiology and Infectious Diseases with Clinic Faculty of Veterinary MedicineUniversity of ZagrebZagreb 10000Croatia Poultry Centre Croatian Veterinary InstituteZagreb 10000Croatia Department of Gastroenterology and Hepatology University Hospital ZagrebZagreb 10000Croatia

BACKGROUND Serologic cross-reactivity between hantaviruses often complicates the interpretation of the *** To analyze the diagnostic value of indirect immunofluorescence assay(IFA)and western blot(WB)in the diagnosis of hantavirus *** One hundred eighty-eight serum samples from Puumala(PUUV)and Dobrava(DOBV)orthohantavirus infected patients were *** was performed using commercial tests(Euroimmun,Lübeck,Germany).RESULTS Using IFA,49.5%of acute-phase samples showed a monotypic response to PUUV,while 50.5% cross-reacted with other *** overall cross-reactivity was higher for immunoglobulin G(IgG)(50.0%)than for immunoglobulin M(IgM)(25.5%).PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan(12.3%/31.5%),Seoul(7.5%/17.8%),DOBV(5.4%/28.1%),and Saaremaa(4.8%/15.7%).Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan(76.2%/95.2%),Saaremaa(80.9%/83.3%),and Seoul(78.6%/85.7%)and moderate with PUUV(28.5%/38.1%).Using a WB,serotyping was successful in most cross-reactive samples(89.5%).CONCLUSION The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections,confirming serotype in most IFA cross-reactive samples.

关键词： Hantaviruses Serology Cross-reactivity Indirect immunofluorescence Western blot

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Detection of HVT FC 126 in lung tissue of chickens vaccinated by means of nebulization against Marek’s disease

Detection of HVT FC 126 in lung tissue of chickens vaccinate...

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The 15th World Veterinary Poultry Congress

作者： Z.Gottstein irena Ciglar Grozdanic Z.Cvetic H.Mazija Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia

＜正＞Marek’s disease（MD）is one of the most important diseases that endanger poultry production worldwide *** every long fattening chicken,but certainly all parent or layer,which leaves the hatchery,must be *** the...

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用离子对液相色谱法测定工业水体中的铀

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国外铀金地质 1992年第4期8卷 84-87页

作者： irena Jancarova 许孙曲

在吸附十二烷基硫酸铵改进的Separon SGXC18填充玻璃柱(3mm×150mm)上,用离子对液相色谱法使U(Ⅵ)与Th(Ⅳ)、Zr(Ⅳ)、Al(Ⅲ)、Fe(Ⅲ)、镧系元素和其他离子分离后,可以有效地测定0.3—1.0mol/L范围的U(Ⅵ)。用异箱洗脱法或pH或浓度梯度... 详细信息

在吸附十二烷基硫酸铵改进的Separon SGXC18填充玻璃柱(3mm×150mm)上,用离子对液相色谱法使U(Ⅵ)与Th(Ⅳ)、Zr(Ⅳ)、Al(Ⅲ)、Fe(Ⅲ)、镧系元素和其他离子分离后,可以有效地测定0.3—1.0mol/L范围的U(Ⅵ)。用异箱洗脱法或pH或浓度梯度洗脱法,采用2-羟基-2-甲基丙酸铵或柠檬酸铵溶液,可以在分析交换柱上直接从酸性水溶液预富集微量铀并与Th、Zr、Al,Fe、镧系元素和其他元素分离,富集因子约为100,回收率为98±8％。利用pH2.7的0.1mol/L甲酸盐缓冲液中后柱与25μmol/L偶氮砷Ⅲ的衍生作用来检测和定量。

关键词：工业用水铀液相色谱法测定

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Intrahepatic expression of genes related to metabotropic receptors in chronic hepatitis

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World Journal of Gastroenterology 2012年第31期18卷 4156-4161页

作者： Andrzej Ciesla Maciej Kusmider Agata Faron-Górecka Marta Dziedzicka-Wasylewska Monika Bocisga-Jasik Danuta Owczarek irena Cieko-Michalska Dorota Cibor Tomasz Mach Department of Gastroenterology Hepatology and Infectious DiseasesJagiellonian University Medical College31-521 KrakówPoland Institute of Pharmacology Polish Academy of Sciences31-343 KrakówPoland

AIM： To screen for genes related to metabotropic re- ceptors that might be involved in the development of chronic hepatitis. METHODS： Assessment of 20 genes associated with metabotropic receptors was performed in liver speci- mens obtained by punch biopsy from 12 patients with autoimmune and chronic hepatitis type B and C. For this purpose, a microarray with low integrity grade and with oligonucleotide DNA probes complementary to target transcripts was used. Evaluation of gene expression was performed in relation to transcript level, correlation between samples and grouping of clinical parameters used in chronic hepatitis assessment. Clini- cal markers of chronic hepatitis included alanine and aspartate aminotransferase, ~,-glutamyltranspeptidase, alkaline phosphatase and cholinesterase activity, levels of iron ions, total cholesterol, triglycerides, albumin, glucose, hemoglobin, platelets, histological analysis of inflammatory and necrotic status, fibrosis according to METAVIR score, steatosis, as well as anthropometric body mass index, waist/hip index, percentage of adi- pose tissue and liver size in ultrasound examination. Gender, age, concomitant diseases and drugs were also taken into account. Validation of oligonucleotide microarray gene expression results was done with the use of quantitative real-time polymerase chain reaction （qRT-PCR）. RESULTS： The highest （0.002 〈 P 〈 0.046） expres- sion among genes encoding main components of metabotropic receptor pathways, such as the a subunit of G-coupled protein, phosphoinositol-dependent pro- tein kinase or arrestin was comparable to that of an- giotensinogen synthesized in the liver. Carcinogenesis suppressor genes, such as chemokine ligand 4, tran- scription factor early growth response protein 1 and lysophosphatidic acid receptor, were characterized by the lowest expression （0.002 〈 P 〈 0.046）, while the factor potentially triggering hepatic cancer, transcrip- tion factor JUN-B, had a 20-fold higher expression. T

关键词： Metabotropic receptors Gene expression DNA oligonucleotides Quantitative real-time poly-merase chain reaction Chronic hepatitis

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