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Comparison of indirect immunofluorescence and western blot method in the diagnosis of hantavirus infections

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World Journal of Methodology 2021年第6期11卷 294-301页

作者： Tatjana Vilibic-Cavlek Ljubo Barbic Vladimir Stevanovic Vladimir Savic Anna Mrzljak Maja Bogdanic irena Tabain School of Medicine University of ZagrebZagreb 10000Croatia Department of Virology Croatian Institute of Public HealthZagreb 10000Croatia Department of Microbiology and Infectious Diseases with Clinic Faculty of Veterinary MedicineUniversity of ZagrebZagreb 10000Croatia Poultry Centre Croatian Veterinary InstituteZagreb 10000Croatia Department of Gastroenterology and Hepatology University Hospital ZagrebZagreb 10000Croatia

BACKGrOUND Serologic cross-reactivity between hantaviruses often complicates the interpretation of the *** To analyze the diagnostic value of indirect immunofluorescence assay(IFA)and western blot(WB)in the diagnosis of hantavirus *** One hundred eighty-eight serum samples from Puumala(PUUV)and Dobrava(DOBV)orthohantavirus infected patients were *** was performed using commercial tests(Euroimmun,Lübeck,Germany).rESULTS Using IFA,49.5%of acute-phase samples showed a monotypic response to PUUV,while 50.5% cross-reacted with other *** overall cross-reactivity was higher for immunoglobulin G(IgG)(50.0%)than for immunoglobulin M(IgM)(25.5%).PUUV IgM/IgG antibodies showed low/moderate reactivity with orthohantaviruses Hantaan(12.3%/31.5%),Seoul(7.5%/17.8%),DOBV(5.4%/28.1%),and Saaremaa(4.8%/15.7%).Both DOBV IgM and IgG antibodies were broadly reactive with Hantaan(76.2%/95.2%),Saaremaa(80.9%/83.3%),and Seoul(78.6%/85.7%)and moderate with PUUV(28.5%/38.1%).Using a WB,serotyping was successful in most cross-reactive samples(89.5%).CONCLUSION The presented results indicate that WB is more specific than IFA in the diagnosis of hantavirus infections,confirming serotype in most IFA cross-reactive samples.

关键词： Hantaviruses Serology Cross-reactivity Indirect immunofluorescence Western blot

Detection of HVT FC 126 in lung tissue of chickens vaccinated by means of nebulization against Marek’s disease

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Detection of HVT FC 126 in lung tissue of chickens vaccinate...

The 15th World Veterinary Poultry Congress

作者： Z.Gottstein irena Ciglar Grozdanic Z.Cvetic H.Mazija Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia Department of Poultry Diseases with Clinic Faculty of Veterinary Medicine University of ZagrebZagrebCroatia

＜正＞Marek’s disease（MD）is one of the most important diseases that endanger poultry production worldwide *** every long fattening chicken,but certainly all parent or layer,which leaves the hatchery,must be *** the...

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用离子对液相色谱法测定工业水体中的铀

国外铀金地质 1992年第4期8卷 84-87页

作者： irena Jancarova 许孙曲

在吸附十二烷基硫酸铵改进的Separon SGXC18填充玻璃柱(3mm×150mm)上,用离子对液相色谱法使U(Ⅵ)与Th(Ⅳ)、Zr(Ⅳ)、Al(Ⅲ)、Fe(Ⅲ)、镧系元素和其他离子分离后,可以有效地测定0.3—1.0mol/L范围的U(Ⅵ)。用异箱洗脱法或pH或浓度梯度... 详细信息

在吸附十二烷基硫酸铵改进的Separon SGXC18填充玻璃柱(3mm×150mm)上,用离子对液相色谱法使U(Ⅵ)与Th(Ⅳ)、Zr(Ⅳ)、Al(Ⅲ)、Fe(Ⅲ)、镧系元素和其他离子分离后,可以有效地测定0.3—1.0mol/L范围的U(Ⅵ)。用异箱洗脱法或pH或浓度梯度洗脱法,采用2-羟基-2-甲基丙酸铵或柠檬酸铵溶液,可以在分析交换柱上直接从酸性水溶液预富集微量铀并与Th、Zr、Al,Fe、镧系元素和其他元素分离,富集因子约为100,回收率为98±8％。利用pH2.7的0.1mol/L甲酸盐缓冲液中后柱与25μmol/L偶氮砷Ⅲ的衍生作用来检测和定量。

关键词：工业用水铀液相色谱法测定

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疼痛药市场激战正酣

中国处方药 2010年第9期9卷 50-51页

作者： irena Melnikova 石永进

新产品进入疼痛市场的策略主要包括：已有镇痛成分新剂型的改进或者采用新的给药系统；新作用机制药物的开发；新适应证的拓展和患者人群的细分。

关键词：药市场疼痛给药系统镇痛成分新适应证新剂型

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公共软空间

建筑与文化 2007年第4期 38-39页

作者： irena Latek Fannie Duguay-Lefebrvre

将城市看作一种性格，设计中采用了动感形象，以建筑语言诠释项目空间，勾勒了建筑、电影的界面。这种设计方式在表现动感形式的同时，展现了公共空间叙述和记事的主题。

关键词：公共空间建筑语言设计方式动感

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美容院巧用水池做装饰

美容院 2006年第5期 72-74页

作者： irena 李京建（摄影）陈艳（摄影）

如今许多不同规模的美容院和女子会馆．都开始在装饰装修上大做文章．虽然这些美容院的规模大小不一．但每个店都想拥有较为突出的特点。其实．若能巧妙运用水池来做装饰．则能简单快速地促成美容院渴望的自然清新、典雅幽静的氛围。

关键词：装饰装修美容院水池

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抗凝药物市场分析及预测

中国处方药 2009年第5期8卷 86-87页

作者： irena Melnikova1 梁伟

抗凝药市场预计将从2008年的60亿美元左右增加至2014年的90亿美元。市场扩容的原因包括人口老龄化以及心血管疾病发病率的增加,但主要是由于新药的上市,这将大大改善现在的治疗状况。

关键词：市场分析抗凝药物预测疾病发病率人口老龄化药市场心血管美元

Dubin-Johnson syndrome coinciding with colon cancer and atherosclerosis

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World Journal of Gastroenterology 2013年第6期19卷 946-950页

作者： Eva Sticova Milan Elleder Helena Hulkova Ondrej Luksan Martin Sauer irena Wunschova-Moudra Jan Novotny Milan Jirsa Center for Experimental Medicine Institute for Clinical and Experimental Medicine14021 Prague 4Czech Republic Third Faculty of Medicine Charles University10000 Prague 10Czech Republic Institute of Inherited Metabolic Disorders First Faculty of MedicineCharles University and VFN12808 Prague 2Czech Republic Department of Surgery IPVZ Masaryk Hospital40113 Usti nad LabemCzech Republic Department of Thoracic Surgery Masaryk Hospital40113 Usti nad LabemCzech Republic Gastroenterology Department Masaryk Hospital40113 Usti nad LabemCzech Republic

Hyperbilirubinemia has been presumed to prevent the process of atherogenesis and cancerogenesis mainly by decreasing oxidative ***-Johnson syndrome is a rare,autosomal recessive,inherited disorder characterized by biphasic,predominantly conjugatedhyperbilirubinemia with no progression to end-stage liver *** molecular basis in Dubin-Johnson syndrome is absence or deficiency of human canalicular multispecific organic anion transporter MrP2/cMOAT caused by homozygous or compound heterozygous mutation(s) in ABCC2 located on chromosome *** onset of the syndrome is most often seen in the late teens or early *** this report,we describe a case of previously unrecognized Dubin-Johnson syndrome caused by two novel pathogenic mutations (c.2360_2366delCCCTGTC and c.3258+1G>A),coinciding with cholestatic liver disease in an 82-year-old male *** patient,suffering from advanced atherosclerosis with serious involvement of coronary arteries,developed colorectal cancer with nodal *** subsequent findings do not support the protective role of Dubin-Johnson type hyperbilirubinemia.

关键词： Dubin-Johnson syndrome ABCC2 Hyperbilirubinemia Oxidative stress Atherosclerosis Cancer

The Importance of the Creation of CEFTA-2006 for the Western Balkans＇ Trade Exchange of Goods

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Chinese Business review 2011年第10期10卷 844-854页

作者： Kikerkova irena Ss Ciril and Methodius University Skopje Republic of Macedonia

By integrating 32 bilateral free trade agreements, CEFTA-2006 became the first multilateral free trade agreement of the Western Balkan countries. Signed at the end of 2006, it was supposed to establish a free trade area among the following member-states: Albania, Bosnia and Herzegovina, Croatia, Macedonia, Moldova, Monte Negro and Serbia.1 The free trade area was supposed to be fully functional by the end of 2010 and should provide trade liberalization by elimination of all tariff and non-tariff barriers for agricultural and non-agricultural goods, as well as trade facilitation by simplification of rules of origin and transit procedures. It should also provide mutual recognition of national sanitary, phytosanitary and technical standards. The free trade area should also enable free trade of services, full protection of intellectual rights, fair rules of public procurement procedures and liberalization of capital investment. The beginning of the creation of the free trade area of the Western Balkan countries created a positive effect upon the increment of the trade exchange of goods among its member-states. However, the weak individual economic capacity of each of them immediately caused a decrement in the exports of goods towards trading partners out of CEFTA-2006. Positive effects realized in the beginning of the creation of the free-trade area were very soon annulated by the influence of the economic crises in 2008. At present, the region experiences a slight, but very slow recovery.

关键词：自由贸易区货物贸易中欧自由贸易协定贸易自由化农产品关税交流创作