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Media Appraisal for Somatic Embryogenesis of Elite Inbred Lines of Maize

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Journal of Life Sciences 2011年第5期5卷 360-363页

作者： inuwa shehu usman shehu Garki Ado Ng Shou Yong Department of Plant Science Ahmadu Bello University PMB 1044 Zaria Nigeria 525/96 （Apt. C20） Rattanakosin View Mansion Bangkok Thailand

Several protocols have been published for somatic embryogenesis in maize. It is essential to compare different protocols on selected germplasm at initial stages of tissue culture to identify a protocol that would yield optimum results. The ultimate goal is to use in vitro system for selecting and generating stress tolerant maize germplasm. Immature embryos of the elite inbred lines of maize （87014 ×Z28-11 and POP43SRS5-57） were cultured on three different semi-solid media （0.5% agar） and the effectiveness of the media for somatic embryogenesis assessed over a period two weeks of sub-culturing. A significant difference was observed among the media in proportion of potentially embryogenic calli and callus cluster size at 1 and 2 weeks after culturing. N6 medium supplemented with casaminoacid （100 mg/L）, 2, 4-dichlorophenoxy acetic acid （1 mg/L）, L-proline （25 mM） and sucrose （2%）, and Murashige and Skoog （MS） medium supplemented with 2, 4-D （2 mg/L） and sucrose （3%） gave higher proportion of potentially embryogenic calli and callus size than MS medium supplemented with casaminoacids （100 mg/L）, 2, 4-D （2 mg/L）, abscissic acid （3.3μM）, and silver nitrate （195 μM） and sucrose （3%）. The difference between genotypes is not significant

关键词： Somatic embryogenesis maize inbreds media silver nitrate

<i>In Situ</i>Germination and Early Seedling Growth of Wormwood (<i>Artemisia annua</i>L.)

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American Journal of Plant Sciences 2014年第11期5卷 1694-1701页

作者： Tahir Salisu Muhammad inuwa shehu usman Maryam Duniya Katung Muhammad Faguji Ishiyaku Department of Biological Sciences Kaduna State University Kaduna Nigeria Department of Plant Science Ahmadu Bello University Zaria Nigeria

Experiments were conducted on the farm of Institute for Agricultural Research (IAR), Ahmadu Bello University, Zaria, during the 2012 hot season and 2013 cold season to determine the most effective treatment for rapid germination of Artemisia annua by subjecting the seeds to physical treatment by soaking in cold and warm water for 2, 4 & 6 hours and 1, 2 & 3 minutes, chemical treatment by soaking in 10%, 20% & 30% Sulphuric Acid (H2SO4) for 1, 2 & 3 minutes respectively and hormone treatment (GA3) by soaking in 100 pp, 200 pp, 300 pp & 500 pp for 6, 12 & 24 hours. Results of Analysis of Variance (ANOVA) indicated no significant difference between the seasons with respect to germination, shoot and root lengths (p ≥ 0.05). However, warm water treatment at 60°C for 2 minutes during the rainy season trial had the least days to germination. Similarly, warm water treatments in both rain and irrigation trials at 40°C for 3 minutes and 60°C for 3 minutes produced the best vigor. Highest germination percent (96%) was observed during the rainy season trial with 10% Sulphuric acid treatment. Using warm water is a simple and affordable treatment especially to local farmers which will give the best result in the germination and seedling production of A. annua. Early March is recommended as the ideal planting period so that seedlings are transplanted on the field at the onset of rainfall.

关键词： Artemisia In Situ Germination Seedling Growth

Predictive modelling of volumetric and Marshall properties of asphalt mixtures modified with waste tire-derived char:A statistical neural

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Journal of Road Engineering 2024年第3期4卷 318-333页

作者： Nura shehu Aliyu Yaro Muslich Hartadi Sutanto Noor Zainab Habib Aliyu usman Abiola Adebanjo Surajo Abubakar Wada Ahmad Hussaini Jagaba Department of Civil and Environmental Engineering Universiti Teknologi PETRONASSeri Iskandar 32610Malaysia Department of Civil Engineering Ahmadu Bello UniversityZaria 810107Nigeria Institute of Infrastructure and Environment Heriot-Watt UniversityDubai 294345United Arab Emirates School of Civil Engineering Central South UniversityChangsha 410075China Interdisciplinary Research Centre for Membranes and Water Security King Fahd University of Petroleum and MineralsDhahran 31261Saudi Arabia

The goals of this study are to assess the viability of waste tire-derived char(WTDC)as a sustainable,low-cost fine aggregate surrogate material for asphalt mixtures and to develop the statistically coupled neural network(SCNN)model for predicting volumetric and Marshall properties of asphalt mixtures modified with *** study is based on experimental data acquired from laboratory volumetric and Marshall properties testing on WTDCmodified asphalt mixtures(WTDC-MAM).The input variables comprised waste tire char content and asphalt binder *** output variables comprised mixture unit weight,total voids,voids filled with asphalt,Marshall stability,and *** coupled neural networks were utilized to predict the volumetric and Marshall properties of asphalt *** predictive modeling,the SCNN model is employed,incorporating a three-layer neural network and preprocessing techniques to enhance accuracy and *** optimal network architecture,using the collected dataset,was a 2:6:5 structure,and the neural network was trained with 60%of the data,whereas the other 20%was used for cross-validation and testing *** network employed a hyperbolic tangent(tanh)activation function and a feed-forward *** to the results,the network model could accurately predict the volumetric and Marshall *** predicted accuracy of SCNN was found to be as high value>98%and low prediction errors for both volumetric and Marshall *** study demonstrates WTDC's potential as a low-cost,sustainable aggregate *** SCNN-based predictive model proves its efficiency and versatility and promotes sustainable practices.

关键词： Waste tire Neural network Sustainable practices Asphalt mixtures Predictive model

Genetics of carotenoids for provitamin A biofortification in tropical-adapted maize

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The Crop Journal 2016年第4期4卷 313-322页

作者： Alhassan D.Halilu shehu G.Ado Daniel A.Aba inuwa S.usman Department of Plant Science Institute for Agricultural Research Ahmadu Bello University

Yellow maize contains high levels of β-carotene(βC), making it an important crop for combating vitamin A deficiency through biofortification. In this study, nine maize inbred lines were selected at random from 31 provitamin A(PVA) maize inbred lines and crossed in a partial diallel mating design to develop 36 crosses. The crosses were evaluated in the field in two locations(Samaru and Kerawa) and their seed carotenoid content were determined by high-performance liquid chromatography. The modes of gene action, heritability, and correlations between agronomic traits and carotenoid content were estimated. Additive genetic variances(σ~2a) were lower than non-additive genetic variances(σ~2d) for all the carotenoids, plant height(PH), and grain yield(GY), suggesting a preponderance of non-additive gene action. Broad-sense heritability(H^2) was high(H^2> 60%) for zeaxanthin,days to anthesis, and PH, moderate(30% < H^2< 60%) for lutein and GY, and low(H^2< 30%)for alpha carotene, beta cryptoxanthin, βC, and PVA. Genetic advance as a percentage of mean, considered with H^2, also suggests a preponderance of non-additive gene action for PVA carotenoids. Hybrid variety development is thus an appropriate approach to improving grain yield and PVA. GY showed no significant genotypic correlations with carotenoid content, suggesting that these traits can be improved concurrently. Thus, there is ample scope for improvement of PVA and GY in the sample of tropical-adapted maize.

关键词： Biofortification Tropical-adapted maize Provitamin A Carotenoids Gene actions Correlations

In Vitro Regeneration of Commercial Sugarcane （Saccharum spp.） Cultivars in Nigeria

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Journal of Life Sciences 2012年第7期6卷 721-725页

作者： Sani Lawan Abdu Mustapha Yahaya usman inuwa shehu College of Engeneering Science Technology Federal Polytechnic Kazaure PMB 5004 Kazuare Nigeria Department of Biological Sciences Bayero University Kano PMB 3011 Kano Nigeria Department of Plant Science Institute for Agricultural Research Ahmadu Bello University PMB 1044 Zaria Nigeria

The effect of different concentrations of 6-benzylaminopurine （BA） with or without 0.2 mg/L NAA on in vitro regeneration of sugarcane （Saccharum spp.） cultivars SP726180, B47419, M1176/77 and M2119/88 were evaluated. Leaf base explants were cultured on Murashige and Skoog （MS） basal medium supplemented with 3.0 mg/L 2,4-dichlorophenoxyacetic acid （2,4-D） for 4 weeks. Thereafter, induction of somatic embryogenesis was observed following the transfer of resulting calli to 2,4-D-free medium for another 4 weeks. Regeneration was achieved by transfer of the embryogenic calli to regeneration media fortified with different concentrations of BA ＋ tt-naphthylacetic acid （NAA）. The number, length and vigor of shoots produced in all the genotypes were highest on media supplemented with 1.0 and 1.5 mg/L BA with and without 0.2 mg/L NAA. Among the genotypes tested, B47419 and M1176/77 recorded highest number of shoots, while maximum shoot length and crop vigor was obtained with M1176/77. Induction of callus with 3.0 mg/L 2,4-D and its subsequent incubation on 2,4-D-free media, followed by regeneration on media supplemented with 1.0 or 1.5 mg/L BA with 0.2 mg/L NAA was found to be efficient for in vitro regeneration of the sugarcane genotypes used in this study. This protocol could be applied for micropropagation of other elite genotypes.

关键词： Saccharum spp. leaf explant 2 4-D callus BA NAA regeneration.

Determinants of Financial Reporting Quality： An In-Depth Study o f Firm Structure

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Journal of Modern Accounting and Auditing 2012年第11期8卷 1656-1672页

作者： shehu usman Hassan Ahmadu Bello University Zaria Nigeria

This paper examines the effects of firm structure on the financial reporting quality of Nigerian-quoted manufacturing firms by using assets, the leverage, the share dispersion, and the residuals of the modified Edward Bell Ohlson （EBO） model as proxies of firm structure and the financial reporting quality respectively. The data are extracted from 12 sample firms representing 50% （as the population of the study） of all the quoted manufacturing companies in Nigeria. A multiple regression is used as a tool of analysis for the study. The result reveals a strongly positive relationship between the firm structure and the financial reporting quality of the quoted manufacturing firms in Nigeria. Therefore, it is recommended among others that the regulators should enforce more on the financial statement disclosure and transparency among companies quoted on the Nigerian Stock Exchange （NSE）, in order to ensure a higher quality of the financial reporting

关键词： firm structure financial reporting quality quoted manufacturing firms

Determinants of Capital Structure in the Nigerian Listed Insurance Firms

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Chinese Business Review 2012年第7期11卷 672-681页

作者： shehu usman Hassan Ahmadu Bello University Zaria Nigeria.

The paper investigates the determinants of capital structure in Nigerian listed insurance firms using data obtained from annual report of the sampled firms for the period of 2001-2010. It used five explanatory variables to measure their effects on debt ratio. Multiple regression is employed as a tool of analysis. The result reveals that all the explanatory variables have statistically and significantly influenced the explained variable. The results approve the prediction of pecking order theory in the case of profitability and trade-off theory in case of tangibility variables. The growth variable supports the agency theory hypothesis whereas size variable confirms to the asymmetry of information theory. It is therefore recommended that the management of listed insurance firms in Nigeria should always consider their positions using these capital structure determinants as important inputs before embarking on debt financing decision.

关键词： capital structure determinants leverage Nigerian insurance firms

Application and circular economy prospects of palm oil waste for eco-friendly asphalt pavement industry:A review

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Journal of Road Engineering 2022年第4期2卷 309-331页

作者： Nura shehu Aliyu Yaro Muslich Hartadi Sutanto Noor Zainab Habib Madzlan Napiah Aliyu usman Ahmad Hussaini Jagaba Abdulnaser M.Al-Sabaeei Department of Civil and Environmental Engineering Universiti Teknologi PETRONASBandar Seri Iskandar 32610Malaysia Department of Civil Engineering Ahmadu Bello UniversityZaria 810107Nigeria Institute of Self-Sustainable Building Universiti Teknologi PETRONASBandar Seri Iskandar 32610Malaysia School of Energy GeoscienceInfrastructureand SocietyHeriot-Watt University DubaiDubai 38103United Arab Emirates

During the production of palm oil,a significant amount of waste is ***,because of inefficient handling and utilization,these wastes are becoming a larger *** a result,one initiative is to use these wastes in the pavement industry as sustainable ***,there is still a lack of understanding about the wider incorporation of palm oil waste in asphalt pavement and its *** study examines existing literature on the use of various wastes in the pavement industry,including palm oil clinker(POC),palm oil fibre(POF),palm kernel shell(PKS),and palm oil fuel ash(POFA).As a result,this paper presents a systematic review and scientometric investigation of related study publications on many uses of palm oil waste in the asphalt pavement industry and its performance from 2009 to *** VOS viewer application was used to conduct the sciento-metric study *** relationship between interactions detected in co-authored country studies cited sources of co-citation,and the keyword of the co-occurrence and publication source enabled the identification of the research *** to the systematic literature review,40%–60% POC can be used to fine aggregate for optimal performance,while 0–100%PKS can be used to replace coarse *** addition,50%–80% POFA or POC fine(POCF)can be used as a filler replacement,5%–8% POCF or POFA as a bitumen modifier,and 0.3% POF as a stabilizing ***,the study demonstrates that the safety of utilizing wastes with more than 50% CO_(2) emissions can be curtailed with minimal heavy metal leaching and radioactivity *** scientometric analysis may encourage researchers to seek out gaps in the literature that will aid in the long-term,multifaceted use of palm oil wastes in the asphalt pavement ***,the study recommends employing and researching the enormous potential of using palm oil waste in the pavement sectors because they are more sustainable and have better perfor

关键词： Asphalt pavement Circular economy Eco-friendly Palm oil waste VOSviewer

Drugs Supply and Laboratory Services in Dots System of Kaduna State: A Health Care Worker Perspective

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Journal of Tuberculosis Research 2018年第1期6卷 19-35页

作者： shehu usman Adamu D. McGill Biological Sciences Kaduna State University Kaduna Nigeria University of Liverpool Liverpool UK

A study was conducted in Kaduna State Nigeria to ascertain the impact of Drug Supply and laboratory services towards effective functioning of DOTS system. Six DOTS providing centres were identified for the study and in each centre, three respondents were selected based on their profession, the Medical Officer, the Pharmacist and the Laboratory Technician totaling 18. The officers were interviewed for the purpose of the research. Semi structured Interviews were conducted in this research as a means of obtaining Health workers perspective. The Interview which was conducted in their respective work station was open ended and all questions are same for all respondents. During the course of the study, all respondents agreed that the sources of drugs supply to their respective centres was the Kaduna State Tuberculosis and leprosy control Program office from the state capital and that drugs are been supplied quarterly and are adequate with slight interruption due to Logistic while for Laboratory services the respondents were of the view that there is the need to improve on it as there are cases of shortage of reagents and erratic supply of Electricity for effective Laboratory functioning.

关键词： Drug Supply and Laboratory Services Workers Perspective