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Isolation and functional characterization of the C-terminus of rice phos-phatidylinositol 4-kinase in vitro

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Cell Research 2003年第2期13卷 131-139页

作者： XIANG FENG KONG, ZHI HONG XU, HONG WEI XUE, National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, Shanghai institutes for Biological Science (SIBS), The Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China 1National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS)The Chinese Academy of Sciences Partner Group el Max-Planck-institute of Molecular plant physiology (MPI-MP) on “plant Molecular physiology and Signal Transduction” 300 Fenglin Road 200032 Shanghai China

A partial rice (Oryza sativa L.) cDNA clone. OsPMK1c, was isolated through screening of a cDNA library constructed from tillering materials. OsPI4Klc encoded a peptide of 608 amino acids with a calculated molecular mass of 68.4 kDa. The OsPI4Klc peptide shared high homology and possessed the highly conserved domains present in most isolated cloned PI4-kinases, i.e. a lipid kinase unique (LKU) domain and a catalytic (CAT) domain. A region with similarity to pleckstrin homology (PH) domain was present in OsPI4K1c as well. Further comparison with genomic sequences in databases revealed that OsPI4K1c is located at the 3'-end of a putative rice PI 4-kinase coding gene OsPI4K1, and its coding region corresponded to the C-terminal half of OsPI4Kl protein. Twelve exons (49-562 bp in size) and 11 introns (77-974 bp in size) were identified in OsPI4K1c. The recombinant protein expressed in Escherichia coli phosphorylates phosphatidylinositol at the D4 position of the inositol ring. OsPI4K1 transcript levels were

关键词： Oryza sativa phosphatidylinositol 4-kinase.

DNA chip-based expression profile analysis indicates involvement of the phosphatidylinositol signaling pathway in multiple plant responses to hormone and abiotic treatments

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Cell Research 2004年第1期14卷 34-45页

作者： Wen Hui LIN, Rui YE, Hui MA, Zhi Hong XU, Hong Wei XUE National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, Shanghai institutes for Biological Sciences (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduction", 300 Fenglin Road, 200032 Shanghai, China Xue HW Chinese Acad Sci Shanghai Inst Biol Sci Inst Plant Physiol & Ecol Natl Lab Plant Mol Genet 300 Fenglin Rd Shanghai 200032 Peoples R China

The phosphatidylinositol (PI) metabolic pathway is considered critical in plant responses to many environmental factors, and previous studies have indicated the involvement of multiple PI-related gene families during cellular responses. Through a detailed analysis of the Arabidopsis thaliana genome, 82 polypeptides were identified as being involved in PI signaling. These could be grouped into different families including PI synthases (PIS), PI-phosphate kinases (PIPK), phospholipases (PL), inositol polyphosphate phosphatases (IPPase), inositol polyphosphate kinases (IPK), PI transfer proteins and putative inositol polyphosphate receptors. The presence of more than 10 isoforms of PIPK, PLC, PLD and IPPase suggested that these genes might be differentially expressed during plant cellular responses or growth and development. Accordingly, DNA chip technology was employed to study the expression patterns of various isoforms. In total, 79 mRNA clones were amplified and used for DNA chip generation. Expression

关键词： phosphatidylinositol signal transduction DNA chip expression profile.

Identification of ABA-responsive genes in rice shoots via cDNA macroar- ray

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Cell Research 2003年第1期13卷 59-68页

作者： FANG LIN, SHOU LING XU, WEI MIN NI, ZHAO QING CHU, ZHI HONG XU, HONG WEI XUENational Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, Shanghai institutes for Biological Science (SIBS), Chinese Academy of Sciences and Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on "plant Molecular physiology and Signal Transduc-tion", 300 Fenglin Road, 200032 Shanghai, China National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Science (SIBS) Chinese Academy of Sciences Partner Group of Max-Planck-institute of Molecular plant physiology (MPI-MP) on plant Molecular physiology and Signal Transduction Shanghai China

Phytohormone abscisic acid (ABA) was critical for many plant growth and developmental processes including seed maturation, germination and response to environmental factors. With the purpose to detect the possible ABA related signal transduction pathways, we tried to isolate ABA-regulated genes through cDNA macroarray technology using ABA-treated rice seedling as materials (under treatment for 2, 4, 8 and 12 h). Of 6144 cDNA clones tested, 37 differential clones showing induction or suppression for at least one time, were isolated. Of them 30 and 7 were up- or down-regulated respectively. Sequence analyses revealed that the putative encoded proteins were involved in different possible processes, including transcription, metabolism and resistance, photosynthesis, signal transduction, and seed maturation. 6 cDNA clones were found to encode proteins with unknown functions. Regulation by ABA of 7 selected clones relating to signal transduction or metabolism was confirmed by reverse transcription PCR. In add

关键词： Oryza saliva abscisic acid (ABA) cDNA macroarray responsive genes signal transduc- tion

Interaction of rice bZIP protein REB with the 5'-upstream region of both rice sbe1 gene and waxy gene

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Chinese Science Bulletin 2002年第4期47卷 310-314页

作者： CAI Yi, XIE Donglu, WANG Zongyang & HONG MengminState Key Laboratory of plant Molecular Genetics, Shanghai institute of plant physiology and ecology, Chinese Academy of Sciences, Shanghai 200032, China State Key Laboratory of plant Molecular Genetics Shanghai Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai China

Rice starch branching enzyme 1 (SBE1) and granule-bound starch synthase (GBSS) catalyzed the bio-synthesis of amylopectin and amylose in rice developing en-dosperm respectively, and the genes encoding these two en-zymes, sbe1 and waxy, are mainly expressed in the en-dosperm. Within the 5 ’-upstream region of rice sbel gene, we identified a 53 bp fragment C53 which could interact with the nuclear proteins extracted from rice endosperm. We also found that fragment Ha-2 in the 5 ’-upstream region of rice waxy gene could compete with C53 for its interaction. Fur-ther experiments demonstrated that rice bZIP protein REB could interact with two ACGT elements (G-box and Hex) in the fragment C53 as well as three ACGT elements (WG1, WG2, and WG3) in the fragment Ha-2. The WG1 element could compete well with Hex element for its interaction with REB. These results suggested that the expression of sbel and waxy gene in rice developing endosperm may be coordinately regulated by REB-like bZIP transcriptional factors.

关键词： rice waxy gene sbel gene bZIP protein.

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Are small RNAs a big help to plants?

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Science China(Life Sciences) 2009年第3期52卷 212-223页

作者： MAO YingBo,XUE XueYi & CHEN XiaoYa National Key Laboratory of plant Molecular Genetics,institute of plant physiology and ecology,Shanghai institutes for Biological Sciences,Chinese Academy of Sciences,Shanghai 200032,China 1. National Key Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200032 China

The discovery of RNA interference(RNAi) has augmented our knowledge of gene regulation and presents a fascinating technology that has a great potential for application in genetic analysis,disease therapy,plant protection,and many other *** this review,we will focus on the biological functions of RNAi and its application in agriculture with a brief introduction to the history of its discovery and molecular mechanism.

关键词： RNA interference gene regulation plant protection

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Regeneration of plants from callus tissues of hairy roots induced by Agrobacterium rhizogenes on Alhagi pseudoalhagi

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Cell Research 2001年第4期11卷 279-284页

作者： WANG YU MEI, JIANG BO WANG, DA LUO , JING FEN JIA National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China Department of Bi institute of plant physiology and ecology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences.

The legume forage Alhagi pseudoalhagi was transformed by the Agrobacterium rhizogenes strain A4 using cotyledon and hypocotyl segments as infection materials. Regenerated plants were achieved from sterile calli derived from hairy roots, which occurred at or near the infection sites. The regenerated plants from hairy root were characterized by normal leaf morphology and stem growth but a shallow and more extensive root system than normal plants. Opine synthesis, PCR and Southern blot confirmed that T- DNA had been integrated into the A. pseudoalhagi genome. Acetosyringone (AS) was found to be vital for successful transformation of A. pseudoalhagi.

关键词： Alhagi pseudoalhagi Agrobacterium rhizogenes hairy root regeneration.

Agrobacterium tumefaciens-mediated transformation of rice with the spider insecticidal gene conferring resistance to leaffolder and striped stem borer

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Cell Research 2001年第2期11卷 149-155页

作者： HUANG JIAN QIU, ZHI MING WEI, HAI LONG AN, YU XIAN ZHU National Laboratory of plant Molecular Genetics, institute of plant physiology and ecology, Shanghai institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032, China National La National Laboratory of plant Molecular Genetics Institute of Plant Physiology and Ecology Chinese Academy of Sciences Shanghai. jqhuang@***

Immature embryos of rice varieties 'Xiushui11' and 'Chunjiang 11' precultured for 4d were infected and transformed by Agrobacterium tumefaciens strain EHA101/pExT7 (containing the spider insecticidal gene). The resistant cant were transferred onto the differentiation medium and plants were regenerated. The transformation frequency reached 56%-72% measured as numbers of Geneticin (G418)-resistant calli produced and 36%-60% measured as numbers of transgenic plants regenerated, respectively. PCR and Southern blot analysis of transgenic plants confirmed that the T-DNA had been integrated into the rice genome. Insect bioassays using T1 transgenic plants indicated that the mortality of the leaffolder (Cnaphalocrasis medinalis) after 7d of leaf feeding reached 38%-61% and the corrected mortality of the striped stem borer (Chilo suppressalis) after 7d of leaf feeding reached 16%-75%. The insect bioassay results demonstrated that the transgenic plants expressing the spider insecticidal protein conferred enhanced resistance to these pests.

关键词： Rice Agrobacterium tumefaciens spider insecticidal gene transgenic plant Leaffolder striped stem borer insect bioassay.

Tissue Distribution and Purification of Prophenoloxidase in Larvae of Asian Corn Borer, Ostrinia furnacalis Guenée (Lepidoptera: Pyralidae)

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Acta Biochimica et Biophysica Sinica 2004年第5期36卷 360-364页

作者： Cong-Jing FENG1,2 and Wen-Jun FU1* 1institute of plant physiology and ecology, Shanghai institute for Biological Sciences, the Chinese Academy of Sciences, Shanghai 200025, China 2The GraduateSchool of the Chinese Academy of Sciences, Beijing 100039, China institute of plant physiology and ecology Shanghai Institute for Biological Sciences the Chinese Academy of Sciences The Graduate School of the Chinese Academy of Sciences

Using ammonium sulphate precipitation, Blue-Sepharose CL-6B, Phenyl-Sepharose CL-4B, prophenoloxidase (PPO) was isolated and purified from hemolymph of Ostrinia furnacalis larvae. This zy- mogen was a heterodimer, and composed of two subunits with the relative molecular mass ranging from 66.2 kD to 97.4 kD determined by SDS-PAGE. Western blotting and indirect immunofluorescence test showed that PPO was present in integument, hemolymph plasma and cell membrane of granular hemocytes and oenocytoids of O. furnacalis larvae.

关键词： Ostrinia furnacalis prophenoloxidase tissue distribution purification

Continuous wavelet transform and discrete multi-resolution analysis of surface fluxes and atmospheric stability

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Progress in Natural Science:Materials International 2006年第4期16卷 403-409页

作者： JIANG Zhaoyang, YU Qiang, WANG Tianduo, SUN Xiaomin and ZHANG Renhua Shanghai institute of plant physiology and ecology, Chinese Academy of Sciences, Shanghai 200032, China institute of Geographic Sciences and Natural Resources Research, Chinese Academy of Sciences, Beijing 100101, China [a]Shanghai institute of plant physiology and ecology Chinese Academy of Sciences Shanghai China [b]institute of Geograhpic Sciences and Natural Resources Research Chinese Academy of Sciences Beijing China

Variations of land surface fluxes of sensible heat (H), latent heat (LE), and CO2(F-CO2) obtained from the eddy-co-variance measurements above a winter wheat field from March 30 to April 24, 2001 have been studied at scales ranging from 10 minutes to days. Wavelet transform is used in the analysis of land surface fluxes and atmospheric stability (ξ) calculated from the measurements to reveal the changes in land surface fluxes in hours to days scales. The main results are: (1) Concise and compact information about the fluxes, net radiation (Rn), temperature ( T) and ξ in the scale-time domain are extracted from the data by continuous wavelet analysis, and 1 day, 0.5 day and short-period (shorter than 0.5 day) components are revealed. Continuous wavelet coefficients can be used to characterize periodic components of changes in fluxes and ξ. (2) Discrete-time multi-resolution analysis can be used to concentrate total energy variance of time series of the measurements to a small number of coefficients, plotting the relative energy distribution to get several meaningful characteristics of the data. (3) Under neutral atmospheric conditions, the relative energy distributions of the Haar multi-resolution analysis of the three non-dimensional coefficients ( T/T* , q/q * and c/c * ) display clear similarities.

关键词： atmospheric stability flux continuous wavelet transform multi-resolution wavelet analysis.