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南京农业大学学报 1989年第3期12卷 66-70页

作者：郭爱珍陈万芳南京市乳牛研究所南京农业大学兽医病理教研组

应用外周全血体外培养法制备染色体标本,通过G-显带,连续C-显带法分析6头屡配不孕的中国黑白花奶牛染色体畸变情况。结果发现:四倍体/二倍体镶嵌3头,染色体臂间倒位2头。四倍体细胞频率(10.30％～16.67％)显著高于对照牛(3.65％)。常规... 详细信息

应用外周全血体外培养法制备染色体标本,通过G-显带,连续C-显带法分析6头屡配不孕的中国黑白花奶牛染色体畸变情况。结果发现:四倍体/二倍体镶嵌3头,染色体臂间倒位2头。四倍体细胞频率(10.30％～16.67％)显著高于对照牛(3.65％)。常规染色并组型分析发现,倒位发生在11号染色体。此外,对照牛中有1头出现染色体罗伯逊易位。常规染色并组型分析证明,核型为59,xx,Robt(2/27)。C-带显示易位染色体为单着丝点。

关键词：奶牛不孕症染色体显带染色体畸变

Infrared Spectroscopy of Blood: A New Tool in Hematology

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Infrared Spectroscopy of Blood: A New Tool in Hematology

FOXP3 expression and clinical characteristics of hepatocellular carcinoma

第九届全国实验血液学会议

作者： Kan-Zhi LIU Research Officer, institute for Biodiagnostics, National Research Council of Canada, and Adjunct Professor, Department of pathology, University of Manitoba Winnipeg, MB, Canada R3B 1Y6

＜正＞ Recent research has demonstrated that infrared（IR）spectroscopy can be extensively applied to varioushaematological research and related clinical problems due to its rapidity,highly reproducibility and capable ofrevealing subtle changes of basic biological molecules before any pathological alteration can be *** cellular components of blood are good candidates for IR studies since they are readilyobtained and purified.A number of encouraging results have been obtained over the last few decades

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World Journal of Gastroenterology 2010年第43期16卷 5502-5509页

作者： Wei-Hua Wang, Jun-Zhi Wang, National Center for Safety Evaluation of Drugs, National institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, Biotechnology Center, School of Pharmacy, the Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Wei-Hua Wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, State Key Laboratory of Cancer Biology, Xi’an 710032, Shaanxi Province, China Wei Yan, Department of pathology, Xijing Hospital, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Yu-Hai Zhang, Department of Health Statistics, The Fourth Military Medical University, Xi’an 710032, Shaanxi Province, China Jiang-Tao Yang, Department of pathology, Xi’an Gaoxin Hospital, Xi’an 710032, Shaanxi Province, China National Center for Safety Evaluation of Drugs National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China.

AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC.

关键词： Forkhead box protein 3 Hepatocellular carcinoma Tumor differentiation Cirrhosis Hepatitis B virus

The Effects of Bone Morphogenetic Protein 2 Gene Transfection on Fibroblasts

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口腔医学研究 2001年第4期17卷 280-283页

作者： SI Xiao-hui 1, YANG Lian-jia 2(1. Shanghai Research institute of Stomatology, The Ninth People's Hospital, Shanghai Second Medical University, Shanghai 200011 2. Department of Oral pathology, Stomatological College, Fourth Military Medical University) Shanghai Research institute of Stomatology The Ninth People's Hospital Shanghai Second Medical University Department of Oral pathology.Stomatological College Fourth Military Medical University

目的 :探讨骨形成蛋白 2 (bonemorphogeneticprotein 2 ,BMP2 )基因转染对成纤维细胞成骨表型的调控作用。方法 :利用脂质体将包含BMP2cDNA全长编码序列的表达载体转染至NIH3T3细胞 ,原位杂交和免疫组化分别检测BMP2在NIH3T3细胞内的稳... 详细信息

目的 :探讨骨形成蛋白 2 (bonemorphogeneticprotein 2 ,BMP2 )基因转染对成纤维细胞成骨表型的调控作用。方法 :利用脂质体将包含BMP2cDNA全长编码序列的表达载体转染至NIH3T3细胞 ,原位杂交和免疫组化分别检测BMP2在NIH3T3细胞内的稳定转染和表达 ,同时观察转染细胞的增殖能力及成骨标志物包括碱性磷酸酶 (alkalinephosphatase ,ALP)活力和骨钙素 (osteocalcin ,OC)含量的变化。结果 :BMP2只在转染细胞内表达。与未转染细胞相比 ,BMP2基因转染细胞的增殖能力降低 ,而ALP活力和OC含量增加。结论 :结果表明BMP2基因转染能够衣导成纤维细胞的体外成骨潜能

关键词：骨形成蛋白转染成纤维细胞分化

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发育特异基因及其在RNA水平上的表达

生物工程学报 1990年第1期6卷 6-10页

作者：金振华宋仁美中国科学院发育生物学研究所伯克利加利福尼亚大学遗传学系

从λ噬菌体gt11的cDNA表达文库筛选到7个胡萝卜体细胞发育特异的基因克隆,并对它们进行了纯化、分离噬菌体DNA,限制酶消化,琼脂糖凝胶电泳鉴定,以及将cDNA插入片段再克隆到pIBI或p^(UC18)质粒等处理。然后,通过Southern blot分析,证明克... 详细信息

从λ噬菌体gt11的cDNA表达文库筛选到7个胡萝卜体细胞发育特异的基因克隆,并对它们进行了纯化、分离噬菌体DNA,限制酶消化,琼脂糖凝胶电泳鉴定,以及将cDNA插入片段再克隆到pIBI或p^(UC18)质粒等处理。然后,通过Southern blot分析,证明克隆16,22,50及60等为新的cDNA基因。此外,又通过Northern blot分析测定了这些基因的组织特异性表达及时序表达。结果表明,克隆22是一种受发育调节,与胡萝卜体细胞胚胎形成密切相关的基因。

关键词： cDNA基因克隆发育特异基因表达

肝细胞肝癌预后与病理特征、PCNA和DNA指数的关系

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复旦学报（医学版） 2002年第2期29卷 87-90页

作者：曾文姣许杰周信达张月娥张农复旦大学医学院病理学教研室复旦大学肝癌研究所上海200032

目的　研究肝细胞肝癌 (HCC)患者预后与肿瘤的病理形态特征、PCNA指数及DNA指数的关系。方法　分析 5 1例中等分化HCC患者的临床、随访资料及病理形态特征 ;应用免疫组织化学方法检测其PCNA阳性指数 ;应用图像分析仪进行DNA指数测量。... 详细信息

目的　研究肝细胞肝癌 (HCC)患者预后与肿瘤的病理形态特征、PCNA指数及DNA指数的关系。方法　分析 5 1例中等分化HCC患者的临床、随访资料及病理形态特征 ;应用免疫组织化学方法检测其PCNA阳性指数 ;应用图像分析仪进行DNA指数测量。结果　癌灶单个 ,直径≤ 5cm ,无癌栓、无肿瘤旁子灶及癌性坏死患者预相对较好 ;DNA整数倍体预后好于非整倍体 ,非整倍体中DI1.5者 ;PCNA指数越高 ,患者预后越差 ;DI与PCNA指数有一定的相关 ,且两者与病理形态特征间也有关联。结论　进行病理形态学和PCNA。

关键词：增殖细胞核抗原 DNA指数肝细胞肝癌预后 pCNA 病理特征

Clinical and pathological correlation of the microdeletion of Y chromosome for the 30 patients with azoospermia and severe oligoasthenospermia

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Asian Journal of Andrology 2004年第4期6卷 369-375页

作者： Han-Sun Chiang Shauh-Der Yeh Chien-Chih Wu Boo-Chung Huang Hui-Ju Tsai Chia-Lang Fang College of Medicine Fu Jen Catholic University Taipei China Department of Urology Taipei Medical University Hospital Taipei China Graduate institute of Medical Sciences Department of pathology Taipei Medical UniversityTaipeiChina

Aim: To review the accumulated 30 patients with different area of Y chromosome microdeletions, focusing on their correlation with the clinical and pathological findings. Methods: A total of 334 consecutive infertile men with azoospermia (218 patients) and severe oligoasthenospermia (116 patients) were screened. Complete physical and endocrinological examinations, general chromosome study and multiplex polymerase chain reaction assay to evaluate the Y chromosome microdeletion were performed. Ten patients received testicular biopsy. Then the clinical and pathological findings were analyzed with reference to the areas of Y chromosome microdeletion. Results: There is a decline of the percentage of sperm appearing in semen in the group that the gene deletion region from AZFc to AZFb. The clinical evidence of the impairment (decreased testicular size and elevated serum FSH) is also relevantly aggravated in this group. However, the pathology of testicular biopsy specimen was poorly correlated with the different deletion areas of the Y chromosome, which may be due to the limited number of specimens. Conclusion: The clinical correlation of spermatogenic impairment to the different AZF deletion regions may provide the information for the infertile couples in pre-treatment counseling.

关键词： chromosome deletion male infertility azoospermia factor azoospermia oligoasthenospermia Y chromosome

The relationship between loss expression of DPC4/Smad4 gene and carcinogenesis of pancreatobiliary carcinoma

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Hepatobiliary & Pancreatic Diseases International 2002年第4期1卷 624-629页

作者： Zhao-Hui Tang Sheng-Quan Zou You-Hua Hao Bao-Ju Wang Xiang-Ping Yang Qi-Qi Chen Fa-Zu Qiu the Department of General Surgery institute of Clinical Immunology Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technologr, Wuhan 430030, China Department of Biochemistry, Rheinisch-Wistfalische Technische Hochschule (RWTH), D-52074 Aachen, Germany Department of pathology, University of lowa, lowa city, IA52242, USA Department of General Surgery Tongji Hospital Huazhong Univ. of Science/Technology Wuhan 430030 China institute of Clinical Immunology Tongji Hospital Huazhong Univ. of Science/Technology Wuhan 430030 China Department of Biochemistry RWTH D-52074 Aachen Germany Department of pathology University of Iowa Iowa City IA 52242 United States

Objective: To clarify the relationship between loss of DPCA gene expression and pathogenesis of pancreato- biliary carcinoma. Methods: 75 slides of normal duct (20), hyperplasia (15), dysplasia (15), invasive carcinoma (25) from patients with pancreatic diseases including pancreatic carcinoma (25 patients), chronic pancreatitis (6), pancreas injury (2) and 71 slides of common bile duct (CBD) carcinoma (38), gallbladder carcinoma (18), hilar bile duct (HBD) carcinoma (15) from patients with primary biliary tract carcinoma were analyzed for the expression of DPC4 protein by im- munohistochemical staining. Results: All specimens from 20 cases of normal duct and 15 cases of hyperplasia showed marked expres- sion of DPC4 protein. The frequency of loss expres- sion of the DPC4 gene was 33 % in dysplasia, and 48% in invasive carcinoma. There was a significant statistical difference between byperplasia and dyspla- sia (P<0.01) and in dysplasia vs invasive carcinoma (P<0.05). The frequency of loss expression of the DPC4 gene was 47.3% in CBD carcinoma, 11% in gallbladder carcinoma, and 13% in HBD carcinoma. The frequency of loss expression of the DPCA gene was significantly different in CBD carcinoma vs gall- bladder carcinoma and HBD carcinoma (P<0.01). Conclusions: Inactivation of the DPC4 gene occurs late in the neoplastic progression of pancreatic carci- noma. The frequency of DPC4 gene alternation was different in various locations of biliary tract carcino- ma. In CBD carcinoma, this frequency is similar to that in pancreatic carcinoma, indicating their similar molecular alternations.

关键词： DPC4 gene pancreatic carcinoma biliary tract carcinoma

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应用昆虫激素类似物增加桑蚕产丝量的研究

昆虫学报 1974年第3期17卷 290-302页

作者： LABORATORY OF PHYSIOLOGY AND pathology, KIANGSU institute OF SERICULTURE SECTION OF SERICULTURAL SERVICE, ACADEMY OF AGRICULTURAL ANDFORESTRIAL SCIENCES OF CHINA LABORATORY OF INSECTICIDE AND TOXICOLOGY, PEKING institute OF ZOOLOGY,ACADEMIA SINICAFIRST RESEARCH LABORATORY, SHANGHAI institute OF ORGANIC CHEMISTRY,ACADEMIA SINICA 中国科学院上海有机化学研究所第一研究室江苏省蚕业研究所生理病理研究室中国科学院北京动物研究所药剂毒理研究室中国农林科学院蚕桑科技服务组

1973年在春季试验的基础上,秋期继续进行了昆虫保幼激素类似物增加桑蚕产丝量的研究,并初步研究了植物性昆虫蜕皮激素与保幼激素的配合使用,结果如下: (1)喷布保幼激素类似物3号后,蚕体叶丝转化率与对照差异不大,这和春季结果相符。五... 详细信息

1973年在春季试验的基础上,秋期继续进行了昆虫保幼激素类似物增加桑蚕产丝量的研究,并初步研究了植物性昆虫蜕皮激素与保幼激素的配合使用,结果如下: (1)喷布保幼激素类似物3号后,蚕体叶丝转化率与对照差异不大,这和春季结果相符。五龄每小时的产茧层量和食下量的成丝率,激素处理区与对照区均无明显差异。食下干物的总量则前老明显超过后者。但喷布激素后的三天左右时间内处理区的食下量却低于对照区;当对照区见熟后,处理区食下量继续增加。故在激素处理后的三天左右时间内,适当控制用桑是增产和减少桑叶消耗的主要关键之一。激素处理后三天左右时间内,绢丝物质的生成和体重增加速度均低于对照区,淀粉酶活性也低于对照区。这与食下量变化的规律一致。蛋白水解酶活性的变化与淀粉酶基本相似,但亦具有自己的特点。 (2)保幼激素类似物3号喷布于五龄蚕体后,茧丝长、茧丝量、解舒丝长及有绪率等均明显增高,拉力和抱合显著改善,切断次数减少,匀度、净度、清洁、茸毛等均与对照区相仿,纤度略有偏租的倾向。以上结果与春季试验一致。多次试验结果亦证明在喷布时间和喷布剂量适当的情况下,对蚕丝解舒并无不良影响,但剂量过高,则有导致解舒率下降的危险。 (3)牛膝蜕皮激素和百日青甾酮都具有缩短龄期的生理活性

关键词：保幼激素类似物剂量喷布激素杀虫剂用药量蜕皮激素甾体激素激素添食桑蚕家蚕昆虫