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食用菌学报 2007年第3期14卷 20-23页

作者： WANG Hong, CHEN MingjieKey Laboratory of agricultural Genetics and Breeding of shanghai institute of edible fungi, shanghai academy of agricultural sciences, shanghai 201106, china

A plasmid containing target DNA and a standard curve for real-time quantitative PCR of the cold-induced Cor3 gene of Volvariella volvacea were constructed. These will provide the basis for further research on Cor3 gene expression at low temperature, and ultimately for assigning a role for the gene in the low temperature autolysis of V. volvacea.

关键词：草菇质粒 PCR Cor3基因冷诱导标准曲线

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Mating System of Stropharia rugoso-annulata

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食用菌学报 2006年第2期 12-14页

作者： WANG Hong, CAO Hui Key Laboratory of agricultural Genetics and Breeding of shanghai institute of edible fungi, shanghai academy of agricultural sciences, shanghai 201106, china

Stropharia rugoso-annulata basidiospores were collected from fruit bodies and single spore clones were isolated. Sixty monokaryon strains were confirmed by the presence of clamp connections and separated by three-cycle spore-crossing tests into four groups containing 6, 14, 21 and 19 strains, respectively. Data indicated that S. rugoso-annulata has a tetrapolar mating system under the control of two genetic factors.

关键词： Stropharia rugoso-annulata Mating system Three-cycle spore-crossing test

Identification of Lentinula edodes ‘135’ Strains Using a Sequence Characterized Amplified Region (SCAR) Marker

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食用菌学报 2006年第3期 8-11页

作者： SONG Chun-yan, TAN Qi, CHEN Ming-jie institute of edible fungi, shanghai academy of agricultural sciences, shanghai 201106, china

The accuracy of a specific Sequence Characterized Amplified Region (SCAR) marker for Lentinula edodes ‘135’ strains, obtained using primers based on RAPD methodology, was confirmed by using the marker to correctly identify nine ‘135’ strains from a test sample of 163 L. edodes strains. Antagonism tests and AFLP amplification were used to further analyze these nine ‘135’ strains, together with strains Qingyuan 135 (Q135) and Fujian 135 (F135), in order to better understand their genetic background. A total of seventeen antagonistic reactions were recorded although the degree of the antagonism observed was generally weak. AFLP analysis revealed a high similarity between the eleven ‘135’ strains, and the average similarity coefficient was 0.9271. Our data demonstrate the feasibility and reliability of the specific SCAR marker for distinguishing ‘135’ strains from other L. edodes strains.

关键词： Identification of Letinula edodes SCAR marker AFLP Antagonism

Characterization of Competitive Mycelial Growth in Pleurotus eryngii

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食用菌学报 2007年第4期 14-18页

作者： SHANG Xiaodong,CHENG Jihong,TAN Qi(shanghai Biotechnology and Engineering Research Center for the edible fungi Key Laboratory of agricultural Geneticsand Breeding institute of edible fungi,shanghai academy of agricultural sciences,shanghai,201106,china)

Competitive mycelial growth(CMG)between Pleurotus eryngii isolates is genetically controlled,and stable CMG responses(positive,neutral or negative)were recorded among the strains *** between CMG responses and mycelial growth rate were *** simultaneously reflects information relating to somatic incompatibility and mycelia growth rate in edible mushrooms,and can be readily and reliably applied to studies on genetic polymorphism among this group of fungi.

关键词： Pleurotus eryngii Competitive mycelial growth Genetic polymorphism

Mating-type Factors of Pleurotus nebrodensis

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食用菌学报 2006年第2期 5-8页

作者： SHANG Xiao-dong, LI Ming-rong, XING Zeng-tao, CAO Hui, TAN Qi Key Laboratory of agricultural Genetics and Breeding of shanghai institute of edible fungi, shanghai academy of agricultural sciences, shanghai 201106, china

Pleurotus nebrodensis has a tetrapolar mating system in which two unlinked incompatible factors (the A and B mating-type factors) controlled the mating of basidiospore-derived monokaryons. In addition to four Mendelian mating types, two new types of B factor originated during sexual reproduction through recombination within the B mating-type factor with a recombination rate of 14.1% . No recombinant A-type factor was observed.

关键词： Mating type Pleurotus nebrodensis Recombination

Cross-breeding and Selection of Flammulina velutipes Hybrid Strains for Industrial Cultivation

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食用菌学报 2008年第4期 31-34页

作者： XU Zhen SHANG Xiaodong SU Rong TAN Qi(Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture,the People's Republic of china shanghai Research Center for edible fungi Biotechnology and Engineering Key Laboratory of agricultural Genetics and Breeding of shanghai institute of edible fungi,shanghai academy of agricultural sciences,shanghai 201106,china)

White Flammulina velutipes strains FM0 F21,F10 and F3-W were selected as the parental strains in a single-spore cross-breeding *** basidiospore-derived monokaryons were selected randomly from each parental strain,and mating was carried out according to the mating groups:FM×F21,FM×F3-W and F3-W×*** analysis revealed that hybrid progenies from the FM×F21 mating group exhibited the highest mating rate (79%).However,only 59.2% of these hybrids produced measurable fruit body yields compared with 82.6% and 84.4% of the hybrids generated from FM×F3-W and F10×F3-W crosses respectively,perhaps indicating that certain hybrid progeny were better adapted to the standard set of cultivation conditions adopted in the *** rates for FM×F3-W and F10 x F3-W crosses were 28% and 42%,respectively indicating that the number of mating type genes may vary among the monokaryons constituting each of the three mating *** growth rates of most FM×F21 hybrid progeny ranged between 4.0～7.0 mm/*** values for progeny from FM×F3-W and F10×F3-W crosses were 3.0～6.0 mm/d and 2.0～6.0 mm/d,respectively although a small number of dikaryons from these two mating groups exhibited much higher/lower growth *** correlation between mycelial growth rate and fruit body yield was evident for any of the three sets of hybrid *** bodies produced by all the hybrid progeny were white in color.

关键词： Flammulina velutipes single-spore cross-breeding mycelium growth rate fruit body yield

Monokaryons Fruiting and Fruit body color of Flammulina velutipes

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食用菌学报 2007年第2期 37-40+91页

作者： YI Ruirong1,2, CAO Hui2, PAN Yingjie31 College of Life sciences, Nanjing agricultural University, Nanjing, Jiangsu 210095, china 2 institute of edible fungi, shanghai academy of agricultural sciences, shanghai 201106, china 3shanghai Fisheries University, shanghai 200090, china

Thirteen kinds of yellow and three kinds of white monokaryons were isolated from mycelium of eight Flammulina velutipes strains using the monokaryon protoplast technique. Of these, five yellow and two white monokaryons produced monokaryotic fruit bodies. Clear differences were observed in the color of the different yellow monokaryotic fruit bodies, as well as between the pilei and stipes of individual yellow monokaryotic fruit bodies, suggesting that the yellow color is under the control of multiple alleles.

关键词： Monokaryotic fruit body Fruiting capacity Fruit body color

Genetic Analysis of Fruit Body Color in a Hybrid Strain of Flammulina velutipes

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食用菌学报 2007年第4期 22-24页

作者： YI Ruirong1,2,CAO Hui1,PAN Yingjie3(1shanghai Biotechnology and Engineering Research Center for the edible fungi Key Laboratory of agriculturalGenetics and Breeding of shanghai institute of edible fungi,shanghai academy of agricultural sciences,shanghai 201106,china 2College of Life sciences,Nanjing agricultural University,Nanjing,Jiangsu 210095,china3shanghai Fisheries University,shanghai 200090,china)

A series of hybrid Flammulina velutipes dikaryons were obtained by crossing yellow and white *** color of fruit bodies generated from these dikaryons,and the ratio between dark-colored stipe regions and the entire stipe of individual fruit bodies,were *** data suggest that the expression of fruit body color in the hybrid dikaryons was under the control of factors present in both the white and yellow parent monokaryons,and that multiple alleles exist in both white and yellow color-controlling factors.

关键词： Fruit body color Color-controlling factors Flammulina velutipes

Cloning,Sequence Analysis and Putative Promoter Region of a Glyceraldehyde-3-Phosphate Dehydrogenase Gene from Volvariella volvacea,and Expression Pattern Under Cold Stress

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食用菌学报 2008年第1期 25-31页

作者： SUN Xiaohong1,2,3,CHEN Mingjie1,PAN Yingjie11 shanghai edible fungi Biotechnology and Engineering Research Center,Key Laboratory of agriculturalGenetics and Breeding of shanghai,institute of edible fungi,shanghai academy of agricultural sciences,shanghai 201106,china 2 College of Life sciences,Nanjing agricultural University,Nanjing,Jiangsu 210095,china 3shanghai Fisheries University,shanghai 200090,china

The complete sequence(from ATG to TAA) of a glyceraldehyde-3-phosphate dehydrogenase(gpd) gene isolated from Volvariella volvacea consisted of 1489 bp and contained nine introns of similar *** full-length cDNA of gpd contained 1157 nucleotides with a predicted ORF of 1011 bp encoding for 337 amino acids and a 3'-untranslated region of 95 *** promoter elements have been identified in a 1220 bp 5'-flanking region,analysis of which revealed two TATA boxes located 80 and 559 bp upstream of the start codon,and three CAAT boxes 333,340 and 406 bp upstream of the initiating *** blot analysis suggested that the *** genome contained only one copy of the gpd *** blot analysis revealed that gpd gene expression was not induced by cold stress,which may explain in part why this mushroom is more perishable than other mushrooms in which gpd expression increases during exposure to low temperatures.

关键词： cold stress gene expression glyceraldehyde-3-phosphate dehydrogenase gene regulatory elements Volvariella volvacea

Inheritance Patterns of a Strain-specific Sequence Characterized Amplified Region (SCAR) Marker for Lentinula edodes Strain 135

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食用菌学报 2008年第4期 6-10页

作者： ZHANG Meiyan~1 SHANG Xiaodong~1 TAN Qi~(1*) CHEN Mingjie~1 PAN Yingjie~2(~1Key Laboratory of Applied Mycological Resources and Utilization,Ministry of Agriculture,the People's Republic of china shanghai Research Center for edible fungi Biotechnology and Engineering Key Laboratory of agricultural Genetics and Breeding of shanghai institute of edible fungi,shanghai academy of agricultural sciences,shanghai 201106,china ~2shanghai Fisheries University,shanghai 200090,P.R.china)

PCR amplification employing a SCAR (Sequence Characterized Amplified Region) primer pair, 135F/R,(amplifying a band of 601 bp) for identifying '135' strains of Lentinula dodes was used to establish the distribution of the specific SCAR marker among 58 protoplast monokaryons and 97 spore monokaryons isolated from a *** 135 *** *** segregated into either A1B1 or A2B2 mating type,and the SCAR marker was detected only in the *** marker was also detected in four delineated mating types (A1B1,A2B2,A1B2,A2B1) of spore *** data testify to the stable inheritance of the specific SCAR marker,and show that the SCAR locus is not genetically linked to either the A or B mating factors.

关键词： Inheritance patterns Lentinula edodes mating type strain-specific sequence characterized amplified region (SCAR) marker