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检索条件"作者=Institute of Cellular and Molecular Biology, Xuzhou Normal University, Xuzhou, Jiangsu 221116, China"

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molecular Genetic Characterization Of MC4R Coding Region In Chinese Cattle Breeds

Molecular Genetic Characterization Of MC4R Coding Region In ...

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第十次全国畜禽遗传标记研讨会

作者： ZHANG Chun-lei, CHEN Hong, LAN Xian-yong, ZHANG Li, ZHANG Ai-ling (College of Animal Science and Technology, Northwest A&F university, Shaanxi Key Laboratory of molecular biology for Agriculture, Yangling, Shaanxi 712100, china institute of cellular and molecular biology, xuzhou normal university, xuzhou, jiangsu 221116, china)

Source/description Melanocortin-4 receptor (MC4R) is one of five protein-coupled receptors binding melanocortins that is implicated in the control of ingestive behavior and energy homeostasis. Mutations have been described in the human and mouse MC4R genes which are associated with obesity. Moreover, a mutation in porcine MC4R is associated with economically important traits in the pig. The SNPs reported in bovine MC4R coding region were specific to breeds. In present experiment over 95% of the coding region of MC4R was screened to detect the SNPs in predominantly cattle breeds of *** conditions The 3 primers of PI(275-764), P2 (485-959) and P3 (804-1126) were designed based upon the bovine MC4R sequence (GenBank accession no. AF265221). The 15 μl polymerase chain reaction (PCR) contained 100 ng of genomic bovine DNA, 10 pmol of each primer, dNTPs (200 μM), Tris-HCl pH 8.8 (20 mM), (NH4)2SO4 (50 mM), MgC12 (1.5 mM), and 0.50 U Taq DNA polymerase (TaKaRa, Dalian, china).The cycling protocol was 4 min at 95℃, 35 cycles of 94℃ for 45 min, Annealing 55-63℃ for 45 s, 72 ℃ for 1 min, with a final extension at 72℃ for 4 min. Single stranded conformation polymorphism (SSCP) Aliquots of 5μl of the PCR products were mixed with 5 μl of the denaturing solution (95% formamide, 25 mM EDTA, 0.025% xylene-cyanole and 0.025% bromophenol blue), heated for 10 min at 98℃ and chilled on ice. Denatured DNA was subjected to PAGE (80×73×0.75 mm) in TBE buffer (89 mM Tris-Borate;2 mM EDTA;pH 8.3) and constant voltage (200V) for 2.5 h. The gel was stained with silver nitrate as previous described *** nucleotide polymorphism (SNP) The PCR fragments from different SSCP patterns were cloned and sequenced. Only two SNPs 927C>T and 1069C>G (GenBank #AF265221) were detected in 509 unrelated cattle from five breeds. The mutations 647T>A, 727G>A, 747G>A were not detected in present populations. This confirmed that these SNPs were specific to breeds. Interestingly, the frequency (Table 1)

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Genetic Variation Of Myostatin Promoter In Nanyang Cattle And Yak

Genetic Variation Of Myostatin Promoter In Nanyang Cattle An...

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第十次全国畜禽遗传标记研讨会

作者： ZHANG Run-feng, CHEN Hong, ZHANG Zheng-feng, ZHANG Hai-jun, YAN Lin-jun, BAO Bin (College of Animal Science and Technology, Northwest A&F university, Shaanxi Key Laboratory of molecular biology for Agriculture, Yangling, Shaanxi 712100, china institute of cellular and molecular biology, xuzhou normal university, xuzhou, jiangsu 221116, china)

Myostatin (GDF-8) acts as a negative regulator of muscle growth. Mutations of the gene are responsible for the double muscling phenotype in several European cattle breeds. In order to further study functions of the bovine myostatin gene, the polymorphisms at promoter region of bovine myostatin gene were detected in Nanyang breed and yak by PCR-RFLPs. The frequencies of the SNP at -371 (T/A) ( relative to ATG translation start codon) were assessed. The allele T dominated in Nanyang breed and yak. No allele A was detected in yak and no homozygous individual with genotype AA was found in Nanyang breed.

关键词： Myostatin gene SNP Nanyang cattle Yak PCR-RFLP

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Association of SNPs in PPARG gene and their haplotypes with body measurements of beef cattle

Association of SNPs in PPARG gene and their haplotypes with ...

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中国畜牧兽医学会第七届养牛学分会2009年学术研讨会

作者： Liushuai Hua~1,Hong Chen~(1,2),Jing Wang~1,Fuying Chen~1,Shenrong Hu~1 (1.College of Animal Science and Technology,Northwest A & F university,Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,china 2.institute of cellular and molecular biology,xuzhou normal university, xuzhou,jiangsu 221116,china.)

PPARG is an important regulator in the regulation of adipose differentiation and development. The mutations of PPARG in human had been proved that associated with type 2 diabetes,fat distribution and body weight.4 SNPs(-110G>C,-27C>T,+20A>G,+1344G>T) were detected in 3 Chinese indigenous cattle breeds(Qinchuan,Nangyang and Jiaxian).The mutations -110G>C,-27C>T and +20A>G located in the Exonl of PPARG,and were under linkage *** mutation +20A>G caused the 7 amino acid changing from Asp to Gly(Asp7Gly).The mutation +1344G>T located in the Exon7 of PPARG,which caused the 448 amino acid changing from Gln to His(Gln448His).The individuals with mutations -27C>T and +20A>G had smaller body weight and body measurements in NY and JX *** no associations were detected between mutation +1344G>T and body weight and body measurements of cattle.

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Genetic analysis of LHX4 gene and its association with economic traits in goats

Genetic analysis of LHX4 gene and its association with econo...

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第十五次全国动物遗传育种学术讨论会

作者： Mijie Li~1,Xianyong Lan~1,Yongjie Jing~1,Gang Ren~1,Tianbao Wei~1,Chuzhao Lei~1,Xin Wang~1, Xingtang Fang~2,Hong Chen~(1,*) 1.College of Animal Science and Technology,Northwest A&F university,Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,china 2.institute of cellular and molecular biology,xuzhou normal university,xuzhou, jiangsu 221116,china

＜正＞Introduction As a member of the LIM homeobox gene family,LHX4 gene plays a critical role in pituitary gland and nervous system *** in the LHX4 gene are associated with CPHD diseases in human and animal models. T... 详细信息

关键词： LHX4 gene PCR-SSCP economic traits goat

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Association of Polymorphisms in the Pit-1 Intron 5 with Growth Traits in Chinese Cattle

Association of Polymorphisms in the Pit-1 Intron 5 with Grow...

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第十次全国畜禽遗传标记研讨会

作者： YANG Dong-ying, CHEN Hong, LAN Xian-yong, ZHANG Zheng-feng, TANG Li-gang, ZHANG Liang-zhi, WANG Xin-zhuang, WANG Yi-min, NIU Hu (College of Animal Science and Technology, Northwest A&F university, Shaanxi Key Laboratory of molecular biology for Agriculture, Yangling, Shaanxi 712100, china institute of cellular and molecular biology, xuzhou normal university, xuzhou, jiangsu 221116, china The Research Center of Beef Cattle Engineering and Technology in Henan, Zhengzhou, Henan 450003, china)

The Pit-1 gene was studied as a candidate for genetic markers of growth traits in Chinese cattle in this study. The single-strand conformation polymorphism method was used to detecte polymorphism in the Pit-1 gene including intron 5. One single nucleotide polymorphism was found in intron 5 of Pit-1. The frequencies of alleles A/B in Nanyang cattle(NY), Qinchuan cattle(QC). Jiaxian Red cattle(JXR), Xizhen cattle(XZ), Luxi cattle(LX) and Holstein cow(H) populations were 0.444/0.556 , 0.477/0.523, 0.538/0.462, 0.421/0.579, 0.523/0.477, 0.475/0.525 respectively. Associations of the polymorphisms with growth traits in Nanyang cattle were analyzed using a general linear model procedure. The following parameters were greater in individuals with genotype AA than with genotype AB: body weight, average daily gain, body height, chest girth at 6, 12, 18 and 24 months(P allele A in other age groups. Therefore, genotype BB maybe a dominant genotype and allele B may be a dominant allele. These results imply that allele B of Pit-1 gene likely positively affects growth traits.

关键词： Beef cattle Growth traits Pit intron 5 Polymorphism

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Bos tauraus and Bos indicus: the Main Ancestress of Cattle Breeds in china Revealed by mtDNA Diversity

Bos tauraus and Bos indicus: the Main Ancestress of Cattle B...

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中国遗传学会功能基因组学研讨会

作者： CHEN Hong, CAI Xin, LEI Chu-zhao, FANG Xing-tang, ZHU Bi-cai, ZHANG Bao (institute of cellular and molecular biology, xuzhou normal university, xuzhou, jiangsu 221116, china College of Animal Science and Technology, Northwest A&F university, Shaanxi Key Laboratory of Agricultural molecular biology, Yangling, Shaanxi 712100, china)

The mitochondrial cyt b genes for 136 Chinese native cattle and D-loop regions for 84 individuals were completely sequenced, which included 105 and 102 variable sites, respectively. The overall nucleotide diversity (0.00960) and haplotype diversity(0.804±0.031) of cyt b genes for Chinese cattle are quite high, so it was with nucleotide diversity(0.02599) and haplotype diversity(0.919±0.027) of D-loop regions. Phylogenetic analysis revealed that all the cattle breeds fall into two distinct lineages: Bos taurus and Bos indicus. Nearly all the Northern cattle breeds were clustered in the clade of Bos taurus in maternal lineage, while most Southern breeds were found to be included in Bos indicus lineage. The significant genetic divergence between Northern and Southern breeds was resulted from the dominant geographical segregation of Qinling Moutains. The cattle breeds in Central Area were also proved to originate from the two progenitors: Bos taurus and Bos indicus, meanwhile, the influence of Bos taurus to Qinchuan cattle and Jinnan cattle was greater than that to the other breeds of Central Area, whereas, Luxi cattle, Jiaxian Red cattle and Nanyang cattle were more greatly influenced by Bos indicus. Jinnan region of Shanxi province and Northwest region of Shandong province were suggested to be the geographically converging area of Bos taurus and Bos indicus.

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Polymorphism of Caprine GHS-R1a Gene and its Association with Growth Traits

Polymorphism of Caprine GHS-R1a Gene and its Association wit...

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第十五次全国动物遗传育种学术讨论会

作者： Q.J.Jin~1,H.Chen~(1,2),C.L.Zhang~1 1.institute of cellular and molecular biology,xuzhou normal university,xuzhou,jiangsu 221116,china 2.College of Animal Science and Technology,Northwest A&F university Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,P.R.china

＜正＞Introdution Growth hormone secretagogue receptor（GHS-R1a） is a G protein-coupled receptor which binds ghrelin and plays an important role in the central regulation of pituitary growth hormone secretion,food intake and energy homeostasis Thus,GHS-R1a gene is a potential candidate gene for caprine growth ***,the roles of genetic variations

关键词： Caprine GHS-R1a gene single-strand conformation polymorphism(SSCP) single nucleotide polymorphism(SNP)

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Association of polymorphisms of the FIT gene with growth traits in cattle

Association of polymorphisms of the FIT gene with growth tra...

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第十五次全国动物遗传育种学术讨论会

作者： Jiajie Sun~1,Chunlei Zhang~1,Xingtang Fang~1,Hong Chen~(1,2x) 1.institute of cellular and molecular biology,xuzhou normal university,xuzhou,jiangsu 221116,china 2.College of Animal Science and Technology,Northwest A&F university,Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,china

＜正＞Introduction Lipid droplet,a unified view of a dynamic *** general,it is composed of a core of neutral lipids, primarily triglycerides（TGs）,surrounded by a monolayer of phospholipids and lipid droplet-associated proteins. Recently,Kaderei et *** reported the characterization of a conserved gene family important for lipid droplet

关键词： FIT gene growth traits mutation SSCP SNP

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The polymorphisms of bovine VEGF gene and their associations with cattle growth traits

The polymorphisms of bovine VEGF gene and their associations...

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中国畜牧兽医学会第七届养牛学分会2009年学术研讨会

作者： Yonghong Pang~1,Chuzhao Lei~1,Chunlei Zhang~2,Xianyong Lan~1,Wangping Yue~2, Chuanwen Gu~2,Danxia Chen~2 and Juqiang Wang~3,Hong Chen~(1*) (1.College of Animal Science and Technology,Northwest A&F university,Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,china 2.institute of cellular and molecular biology,xuzhou normal university, xuzhou,jiangsu 221116,china 3.Research Center of Cattle Engineering Technology in Henan Zhengzhou,Henan 450003,china)

＜正＞Introduction Vascular endothelial growth factor（VEGF） is a key regulator of vascular development in physiological and pathological conditions,with crucial roles in developmental blood vessel formation and hypoxia-induced tissue *** findings demonstrate that VEGF is an essential survival factor for muscle capillarity,bone formation,bone healing and regulation of ***,it may be a potential candidate

关键词： Chinese cattle VEGF gene Single nucleotide polymorphism(SNP)

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Assessment of Paired box 7 gene(Pax7) polymorphisms as markers of growth traits in Chinese beef cattle

Assessment of Paired box 7 gene(Pax7) polymorphisms as marke...

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中国畜牧兽医学会养牛学分会2011年学术研讨会

作者： Yao Xu~1,Jinbiao Liu~1,Xianyong Lan~1,Ya Zhang~1,Chuzhao Lei~1, Xingtang Fang~2,Hong Chen,~* 1 College of Animal Science and Technology,Northwest A & F university,Shaanxi Key Laboratory of molecular biology for Agriculture,Yangling,Shaanxi 712100,china 2 institute of cellular and molecular biology,xuzhou normal university,xuzhou,jiangsu 221116, china

The objectives of this study were to detect single nucleotide polymorphisms(SNPs) of Paired box 7(Pax7) gene in 1226 beef cattle from five Chinese indigenous breeds,and investigate their effects on growth *** novel SNPs were identified,including three synonymous mutations(NC07300: g.3531G>A,g.56626G>A and g.66117C>T) and one intron mutation(g.66251A>G),which were detected by HinfI,BspT104I,PstI,and Apal PCR-RFLP,*** analysis showed genotypes of Hinfl and ApaI loci showed significant effects on body weight and average daily gain of cattle aged 6 months,and PstI genotypes significantly affected chest girth of cattle aged 6 and 12 months(Pmolecular marker in marker-assisted selection(MAS) programs for the beef cattle breeding.

关键词： Pax7 gene SNPs PCR-RFLP Cattle Growth traits

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