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Specific inhibition of Streptococcus bovis by endolysin LyJH307 supplementation shifts the rumen microbiota and metabolic pathways related to carbohydrate metabolism

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Journal of Animal Science and Biotechnology 2022年第1期13卷 209-223页

作者： Hanbeen Kim Tansol Park inhyuk kwon Jakyeom Seo Department of Animal Science Life and Industry Convergence Research InstitutePusan National University1268-50 Samrangjin-roMiryang 50463Republic of Korea Department of Animal Science and Technology Chung-Ang UniversityAnseong-siGyeonggi-do 17546Republic of Korea EASY BIO Inc.Seoul 06253South Korea

Background:Endolysins,the bacteriophage-originated peptidoglycan hydrolases,are a promising replacement for antibiotics due to immediate lytic activity and no antibiotic *** objectives of this study were to investigate the lytic activity of endolysin LyJH307 against *** and to explore changes in rumen fermentation and microbiota in an in vitro *** treatments were used:1)control,corn grain without LyJH307;and 2)LyJH307,corn grain with LyJH307(4 U/mL).An in vitro fermentation experiment was performed using mixture of rumen fluid collected from two cannulated Holstein steers(450±30 kg)and artificial saliva buffer mixed as 1:3 ratio for 12 h incubation *** vitro dry matter digestibility,pH,volatile fatty acids,and lactate concentration were estimated at 12 h,and the gas production was measured at 6,9,and 12 *** rumen bacterial community was analyzed using 16S rRNA amplicon ***:LyJH307 supplementation at 6 h incubation markedly decreased the absolute abundance of ***(approximately 70% compared to control,P=0.0289)and increased ruminal pH(P=0.0335)at the 12 h *** acetate proportion(P=0.0362)was significantly increased after LyJH307 addition,whereas propionate(P=0.0379)was ***307 supplementation increased D-lactate(P=0.0340)without any change in L-lactate concentration(P>0.10).There were no significant differences in Shannon’s index,Simpson’s index,Chao1 estimates,and evenness(P>0.10).Based on Bray-Curtis dissimilarity matrices,the LyJH307 affected the overall shift in microbiota(P=0.097).LyJH307 supplementation induced an increase of 11 genera containing Lachnoclostridium,WCHB1-41,unclassified genus Selenomonadaceae,Paraprevotella,vadinBE97,Ruminococcus gauvreauii group,Lactobacillus,Anaerorhabdus furcosa group,Victivallaceae,Desulfuromonadaceae,and *** predicted functional features represented by the Kyoto Encyclopedia of Genes and Genomes pathways were changed by LyJH307 toward a de

关键词： Endolysin Rumen microbiota Ruminal acidosis Streptococcus bovis

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