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Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

The Crop Journal 2023年第6期11卷 1828-1835页

作者： Jie Li Amanpreet Kaur Brian Harrower Miles Armstrong Daolong Dou Xiaodan Wang ingo hein Department of Plant Pathology and MOA Key Lab of Pest Monitoring and Green Management College of Plant ProtectionChina Agricultural UniversityBeijing 100193China The James Hutton Institute InvergowrieDundee DD25DAUK University of Dundee Dundee DD14HNUK Crop Research Centre TeagascOakParkCarlow R93 XE12Ireland College of Plant Protection Academy for Advanced Interdisciplinary StudiesNanjing Agricultural UniversityNanjing 210095JiangsuChina

More than 170 years after causing the potato famine in Ireland,late blight is still considered one of the most devastating crop *** potato breeding efforts depend on natural sources of resistance to protect crops from the rapidly evolving late blight pathogen,Phytophthora *** have identified and mapped a novel broad-spectrum disease resistance gene effective against *** from the wild,diploid potato species Solanum *** resistance gene enrichment sequencing(dRenSeq)was used to confirm the uniqueness of the identified *** and GenSeq-based mapping of the resistance,referred to as Rpi-blb4,alongside recombinant screening,positioned the locus responsible for the resistance to potato chromosome *** interval spans approximately 2.3 Mb and corresponds to the DM reference genome positions of 11.25 and 13.56 Mb.

关键词： Renseq Disease resistance Late blight Solanum bulbocastanum

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Utilizing the Potato Genome for Applied and Fundamental Research into Diverse Aspects of Disease Resistance

Utilizing the Potato Genome for Applied and Fundamental Rese...

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2019年中国马铃薯大会

作者： Miles Armstong Xinwei Chen Micha Bayer ingo hein Cell and Molecular Sciences The James Hutton Institute School of Life Sciences The University of Dundee

Potato is the most important non-cereal food crop worldwide and consumed by more than a billion *** and pathogens represent a serious and continuing threat to potato *** of the most widespread and economically significant diseases are late blight,nematodes and *** ability to withstand multiple biotic and abiotic stresses is critical for wild *** the availability of significant germplasm resources including the Commonwealth Potato Collection(CPC)at the James Hutton Institute and improved genomics methods,the potential to exploit natural biodiversity is considerable to secure potatoes as a major food source in the *** the potato genome has provided unprecedented insight into the distribution and organisation of genes that underpin *** have developed a gene-specific target enrichment and sequencing platform,RenSeq,to more rapidly map and clone functional NB-LRR genes that are effective against the major potato *** resistance gene enrichment sequencing(dRenSeq)enables the high-confidence identification and complete sequence validation of functional resistance genes in *** have shown that the technology can direct parental selection in potato breeding programs,confirms transgene integrity in GM crops and enables high-throughput allele *** study reveals a very limited base of utilised resistances in all major potato cultivars but has identified additional and currently very effective resistances in potato varieties that could be combined with the help of the *** combine the most complementary resistances,we can assess the diversity of the Phytophthora *** pathogen populations through a similar enrichment-sequencing based approached referred to as *** combination of dRenSeq and PenSeq enables,for the first time,a comprehensive Pathogen/Crop coevolution study on a global scale.

关键词： RenSeq PenSeq plant-pathogen co-evolution NLR gene deployment

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SMRT-AgRenSeq-d in potato (Solanum tuberosum) as a method to identify candidates for the nematode resistance Gpa5

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Horticulture Research 2023年第11期10卷 271-279页

作者： Yuhan Wang Lynn H.Brown Thomas M.Adams Yuk Woon Cheung Jie Li Vanessa Young Drummond T.Todd Miles R.Armstrong Konrad Neugebauer Amanpreet Kaur Brian Harrower Stan Oome Xiaodan Wang Micha Bayer ingo hein Division of Plant Sciences at the Hutton The University of DundeeErrol RoadInvergowrieDundeeDD25DAUK The James Hutton Institute Errol RoadInvergowrieDundeeDD25DAUK College of Plant Protection China Agricultural UniversityHaidian DistrictBeijing100083China James Hutton Limited The James Hutton InstituteErrol RoadInvergowrieDundeeDD25DAUK Biomathematics and Statistics Scotland Errol RoadInvergowrieDundeeDD25DAUK Crop Research Centre TeagascOak ParkCarlow R93 XE12Ireland HZPC Research B.V.HZPC Edisonweg 58501 XG JoureNetherlands

Potato is the thirdmost important food crop in *** pathogens threaten sustainable crop production but can be controlled,in many cases,through the deployment of disease resistance genes belonging to the family of nucleotide-binding,leucine-rich-repeat(NLR)*** identify effective disease resistance genes in established varieties,we have successfully established SMRT-AgRenSeq in tetraploid potatoes and have further enhanced the methodology by including dRenSeq in an approach that we term *** inclusion of dRenSeq enables the filtering of candidates after the association analysis by establishing a presence/absence matrix across resistant and susceptible varieties that is translated into an F1 *** a SMRT-RenSeq-based sequence representation of the NLRome from the cultivar Innovator,SMRT-AgRenSeq-d analyses reliably identified the late blight resistance benchmark genes Rpi-R1,Rpi-R2-like,Rpi-R3a,and Rpi-R3b in a panel of 117 varieties with variable phenotype *** benchmark genes were identified with an F1 score of 1,which indicates absolute linkage in the *** method also identified nine strong candidates for Gpa5 that controls the potato cyst nematode(PCN)species Globodera pallida(pathotypes Pa2/3).Assuming that NLRs are involved in controlling many types of resistances,SMRT-AgRenSeq-d can readily be applied to diverse crops and pathogen systems.

关键词： resistance linkage crops

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A New Approach to Developing Universally Transferable Markers for Disease Resistance Breeding

A New Approach to Developing Universally Transferable Marker...

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2019年中国马铃薯大会

作者： Xinwei Chen Mile R.Armstrong Micha Bayer Brian Harrower ingo hein Cell and Molecular Sciences The James Hutton Institute School of Life Sciences The University of Dundee

Modern molecular technologies enable identification and discrimination of the causal genes for resistance to a variety of pests and *** disease resistance breeding requires efficient tracking and screening for the resistance genes in progenies by employing molecular ***,application of marker assisted selection faces great challenges of limited diagnostics and transferability in potato due to its complex genome of high heterozygosity and *** study aims to tackle the problem through development of a novel type of maker based on diagnostic resistance gene enrichment sequencing(dRenSeq)and assessment for uniqueness of all potential SNPs in comparison with known functional genes across potato *** generated dRenSeq datasets for a total of 136 diverse cultivated and wild potato *** data for three resistance genes R3 a,Nem-Gpa2 and Rx functionally involving in potato resistance to late blight,potato cyst nematode and potato virus X respectively were extracted and *** an extremely stringent threshold with 0% mismatch rate,27,13 and 13 cultivars were detected covering the full reference resistance gene sequences,suggesting presence of the functional resistance genes in these cultivars.A relaxed stringency of 4% mismatch rate was used to explore SNP variations across the dRenSeq panel and identify SNPs with non-resistance nucleotides(NRNs)being universally present in all clones,a criterium adopted for development of universal transferability markers(UTM).One or more SNPs per gene met the criteria as candidate for *** of the candidate SNPs into Kompetitive-allele-specific PCR(KASP)markers faithfully reproduced the dRenseq results and distinguished in 100% success rate clones carrying R-genes from those *** study provides insight into development of UTMs which can be readily extended to other resistance genes and/or different types of functional genes for other traits.

关键词： universally transferrable markers resistance genes marker assisted breeding

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Identification and mapping of Rpi-blb4 in diploid wild potato species Solanum bulbocastanum

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The Crop Journal 2023年第6期

作者： Jie Li Amanpreet Kaur Brian Harrower Miles Armstrong Daolong Dou Xiaodan Wang ingo hein

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