检索结果-南通市图书馆

Rejection of Experimental Hodgkins Lymphoma by T-Cells Engineered with a CD19 Chimeric Antigen Receptor

Journal of Cancer Therapy 2012年第5期3卷 553-561页

作者： Anna Swanson Eleanor Cheadle David Gilham Dorothy Crawford Simon Talbot ingo Johannessen 5Laboratory Medicine (Virology) Royal Infirmary of Edinburgh Edinburgh UK The University of Edinburgh Centre for Infectious Diseases Edinburgh UK The University of Edinburgh Division of Pathway Medicine Chancellor’s Building Edinburgh UK The University of Manchester Clinical and Experimental Immunology Group Manchester UK

T cells engineered to express chimeric antigen receptors (CARs) combining an external antibody binding domain with the CD3ζ T cell receptor (TCR) signaling domain for triggering cell activation are being used for immunotherapeutic targeting of tumor cells in a non-HLA restricted manner. In this study we transduced T cells with a CD19-CAR construct containing a truncated CD34 gene (tCD34) marker and used these to target the B cell antigen CD19 on the surface of a Hodgkin’s lymphoma (HL) cell line (L591) both in vitro and in vivo. Levels of tCD34 expression in transduced peripheral blood mononuclear cells (PBMCs) ranged from 6% - 20% and this was increased to 82% after selection for transduced tCD34+ cells. In vitro cytotoxicity testing on a CD19+ HL cell line (L591) showed specific cell lysis initiated by the CD19-CAR transduced PBMCs. Importantly, CD19-CAR T cells prevented the growth of L591 HL tumor cells when co-injected subcutaneously (sc) in 6/6 severe combined immunodeficient (SCID) mice. There was no evidence of anti-tumor activity when CD19-CAR T cells were infused intravenously (iv) at the same time as L591 HL tumor cells were injected sc. However, 3/6 SCID mice showed tumor rejection within 83 days after iv infusion of CD19-CAR T cells 3 - 9 days after establishment of L591 HL tumors, while all control animals succumbed to tumors within 60 days. Interestingly, immuno-histochemical analysis of L591 HL tumors demonstrated that CD19-CAR T cells were detected not earlier than 11 days after infusion within the tumor mass. These results suggest that CD19 is a potentially attractive target for the immunotherapy of HL.

关键词： Hodgkin’s Lymphoma CD19 Chimeric Antigen Receptor Immunotherapy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Effect of Biofertilizer on Biomass Productivity, Nutrient Balance and Soil Fertility in Rainfed Organic Ginger Production System

引用

Journal of Agricultural Science and Technology(A) 2013年第1期3卷 10-19页

作者： Nongmaithem Jyotsna Mainak Ghosh Dulal Chandra Ghosh, Wahengbam ingo Meitei Jagadish Timsina College of Agriculture Central Agricultural University Imphal Manipur 795001 India Department of Agronomy Bihar Agricultural University Sabour 813210 Bihar India Institute of Agriculture Visva-Bharati Sriniketan 731236 West Bengal India IRRI-Bangladesh Office Banani DOHS Dhaka 1206 Bangladesh

Farmers of North-Eastern India grow ginger organically and obtain low yield. Biofertilizer may help in increasing yield and maintaining soil fertility. An investigation made with different biofertilizers showed that seed treatment with biofertilizer increased biomass by 18.3%, enhanced N, P and K removal and improved short-term soil fertility status by increasing N and P balance and reducing negative K balance over control plots. Use of high dose （5.0 kg haL） of Azotobacter （a3） and medium dose （3.75 kg hal） of both Azospirillum （b2） and Phosphotica （c2） increased rhizome biomass by 6.8%-12.5% and shoot biomass by 5.6%-14.3% over other levels. They enhanced N, P and K removal by both rhizome and shoot when compared with other levels. The above biofertilizer treatments improved organic carbon and available N and P status of the soil by increasing N and P balance. The result showed overall strong negative K balance; but biofertilizer treatments greatly reduced the negative K balance in soil as compared to the control plots. Seed treatment with high level of Azotobacter along with medium level of Phosphotica （a3c2） produced the highest biomass yield （7.4 t hal）, increased N and P balance and fertility status in spite of hizh N, P and K removal.

关键词： Biofertilizer ginger productivity nutrient balance soil fertility.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PLA-Based Biodegradable and Cytocompatible Implant Materials： Material Development, Processing and Properties

引用

材料科学与工程（中英文B版） 2013年第10期3卷 619-632页

作者： Maike Bargmann Hans Kothe Bernd Steinhoff ingo Alig Claudia Rode RalfWyrwa MatthiasSchnabelrauch Fraunhoferlnstitute for Structural Durability and System Reliability LBF Darmstadt 64289 Germany INNOVENTe. V. Priissingstrasse 27B 07745 Jena Germany

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Utilizing the Potato Genome for Applied and Fundamental Research into Diverse Aspects of Disease Resistance

Utilizing the Potato Genome for Applied and Fundamental Rese...

引用

2019年中国马铃薯大会

作者： Miles Armstong Xinwei Chen Micha Bayer ingo Hein Cell and Molecular Sciences The James Hutton Institute School of Life Sciences The University of Dundee

Potato is the most important non-cereal food crop worldwide and consumed by more than a billion *** and pathogens represent a serious and continuing threat to potato *** of the most widespread and economically significant diseases are late blight,nematodes and *** ability to withstand multiple biotic and abiotic stresses is critical for wild *** the availability of significant germplasm resources including the Commonwealth Potato Collection(CPC)at the James Hutton Institute and improved genomics methods,the potential to exploit natural biodiversity is considerable to secure potatoes as a major food source in the *** the potato genome has provided unprecedented insight into the distribution and organisation of genes that underpin *** have developed a gene-specific target enrichment and sequencing platform,RenSeq,to more rapidly map and clone functional NB-LRR genes that are effective against the major potato *** resistance gene enrichment sequencing(dRenSeq)enables the high-confidence identification and complete sequence validation of functional resistance genes in *** have shown that the technology can direct parental selection in potato breeding programs,confirms transgene integrity in GM crops and enables high-throughput allele *** study reveals a very limited base of utilised resistances in all major potato cultivars but has identified additional and currently very effective resistances in potato varieties that could be combined with the help of the *** combine the most complementary resistances,we can assess the diversity of the Phytophthora *** pathogen populations through a similar enrichment-sequencing based approached referred to as *** combination of dRenSeq and PenSeq enables,for the first time,a comprehensive Pathogen/Crop coevolution study on a global scale.

关键词： RenSeq PenSeq plant-pathogen co-evolution NLR gene deployment

来源： cnki会议评论

在线全文

cnki会议

学校读者我要写书评

暂无评论

Induction of the AOX1D Isoform of Alternative Oxidase in A. thaliana T-DNA Insertion Lines Lacking Isoform AOX1A Is Insufficient to Optimize Photosynthesis when Treated with Antimycin A

引用

Molecular Plant 2009年第2期2卷 284-297页

作者： Inga Strodtkotter Kollipara Padmasree Challabathula Dinakar Birgit Speth Pamela S. Niazi Joanna Wojtera ingo Voss Phuc Thi Do Adriano Nunes-Nesi Alisdair R. Fernie Vera Linke Agepati S. Raghavendra Renate Scheibe Department of Plant Physiology FBS University of Osnabrueck 49069 Osnabrueck Germany Department of Plant Sciences School of Life Sciences University of Hyderabad Hyderabad 500 046 India Max Planck Institute for Molecular Plant Physiology Am Muhlenberg-1 14476 Potsdam-Golm Germany

Plant respiration is characterized by two pathways for electron transfer to O2, namely the cytochrome pathway （CP） that is linked to ATP production, and the alternative pathway （AP）, where electrons from ubiquinol are directly transferred to O2 via an alternative oxidase （AOX） without concomitant ATP production. This latter pathway is well suited to dispose of excess electrons in the light, leading to optimized photosynthetic performance. We have characterized T- DNA-insertion mutant lines of Arabidopsis thaliana that do not express the major isoform, AOXIA. In standard growth conditions, these plants did not show any phenotype, but restriction of electron flow through CP by antimycin A, which induces AOXIA expression in the wild-type, led to an increased expression of AOXID in leaves of the aoxla-knockout mutant. Despite the increased presence of the AOX1D isoform in the mutant, antimycin A caused inhibition of photosyn- thesis, increased ROS, and ultimately resulted in amplified membrane leakage and necrosis when compared to the wild- type, which was only marginally affected by the inhibitor. It thus appears that AOX1 D was unable to fully compensate for the loss of AOXIA when electron flow via the CP is restricted. A combination of inhibition studies, coupled to metabolite profiling and targeted expression analysis of the P-protein of glycine decarboxylase complex （GDC）, suggests that the aoxla mutants attempt to increase their capacity for photorespiration. However, given their deficiency, it is intriguing that increase in expression neither of AOX1D nor of GDC could fully compensate for the lack of AOXIA to optimize pho- tosynthesis when treated with antimycin A. We suggest that the aoxla mutants can further be used to substantiate the current models concerning the influence of mitochondrial redox on photosynthetic performance and gene expression.

关键词： abiotic/environmental stress acclimation--physiological alternative electron transport photorespiration photosynthesis mitochondria T-DNA insertion line.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

捻针时真、假穴不同中枢激活效应的脑功能MRI

引用

中华放射学杂志 2004年第12期38卷 1281-1284页

作者：方继良 Timo Krings Juergen Weidemann ingo Meister Armin Thron 中国中医研究院广安门医院放射科 Department of Neuroradiology University Hospital of the University of TechnologyAachen(Pauwelsstrasse3052057AachenGermany) Department of Neuroradiology University Hospital of the University of TechnologyAachen(Pauwelsstrasse3052057AachenGermany Department of Neurology University Hospital of the University of TechnologyAachen(Pauwelsstrasse3052057AachenGermany)

目的　利用功能磁共振 (fMRI)观察捻针针刺方法对大脑的作用。方法　用盲法随机分别针刺 13例正常人左侧太冲、丘墟和假穴 ,手法采用捻针和不捻针 ,利用 1 5TMR仪行全脑功能成像 ,用SPM99b分析图像。结果　发现只在真穴捻针针刺时有更... 详细信息

目的　利用功能磁共振 (fMRI)观察捻针针刺方法对大脑的作用。方法　用盲法随机分别针刺 13例正常人左侧太冲、丘墟和假穴 ,手法采用捻针和不捻针 ,利用 1 5TMR仪行全脑功能成像 ,用SPM99b分析图像。结果　发现只在真穴捻针针刺时有更强的激活效应 ,有显著性意义的Brodmann激活区域为 :针刺太冲穴激活了左右顶叶BA4 0区 ,右额叶BA4 7和 10区 ,右丘脑 ,左小脑 ;针刺丘墟穴激活了左右顶叶BA4 0区 ,右顶叶BA2区 ,左额叶BA9、10、4 4区 ,左岛叶BA13区 ,左颞叶BA2 2区 ,右颞叶BA4 2区 ,右壳核 ,左小脑。两真穴激活部位部分相同 ,部分不同。在假穴捻针对不捻针的研究中 ,未见到有显著性意义的激活区。结论　真穴捻针时可能对皮层神经活动有较特异的作用 ,而假穴未观察到此种现象 ,推测这可能是针刺治疗作用的机制之一。

关键词：激活针刺 MRI 中枢颞叶 BA 脑功能图像 TMR 显著性

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

软件管理实现药品精确配送

引用

现代制造 2009年第40期 80-82页

作者： ingo PALEIT

一家药店配货系统制造商采用了一套通用的企业软件,这套软件可以精确计算个性化的配料,保证交货日期准确无误;而且可以根据需求及时采购材料,并通过快速在线信息调用实现过程高透明度。

关键词：软件管理配送药品自动化技术仓库技术供货商工程师

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

不被污染的工作台

引用

实验与分析 2008年第5期 48-49页

作者： ingo Sternitzke Trespa International BV

对于成功的实验工作来说，实验室的整洁和卫生是最重要的前提条件。为了满足这些条件，荷兰法医研究所采用了具有抗菌特性的实验室板材。

关键词：工作台污染实验工作抗菌特性实验室研究所板材

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

双通道使传感器满足工业4.0的要求

引用

现代制造 2019年第12期 32-33页

作者： ingo Baumgardt 不详

工业4.0给传感技术提出了新的挑战。它必须进一步的提供实时信息,而且也包括了把信息提供给高层次的主控系统。工业4.0或者工业物联网主要关注的是跨所有系统边界、直至云端的数据和数据交换的标准化。大多数数据都是传感器采集到的;而... 详细信息

工业4.0给传感技术提出了新的挑战。它必须进一步的提供实时信息,而且也包括了把信息提供给高层次的主控系统。工业4.0或者工业物联网主要关注的是跨所有系统边界、直至云端的数据和数据交换的标准化。大多数数据都是传感器采集到的;而传感器的主要任务就是捕捉过程参数,并通过接口将采集到的数据传输给接收数据的系统。这些过程参数就是解决实际自动化任务的原始数据。

关键词：传感器工业通道主控系统实时信息过程参数传感技术数据交换

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

固态锂金属电池中不均匀离子通量介导的正负极耦合失效机制

引用

Science Bulletin 2023年第8期68卷 813-825,M0004页

作者：郑越张舒马君孙富 Markus Osenberg AndréHilger Henning Markötter Fabian Wilde ingo Manke 胡中波崔光磊 Qingdao Industrial Energy Storage Research Institute Qingdao Institute of Bioenergy and Bioprocess TechnologyChinese Academy of SciencesQingdao 266101China College of Materials Science and Opto-Electronic Technology University of Chinese Academy of SciencesBeijing 100049China Shandong Energy Institute Qingdao 266101China Institute of Applied Materials Helmholtz-Zentrum Berlin für Materialien und EnergieBerlin 14109Germany Department of Non-Destructive Testing Bundesanstalt für Materialforschung und-PrüfungBerlin 12205Germany Institute of Materials Physics Helmholtz-Zentrum HereonGeesthacht 21502Germany

本文通过联用无损三维同步辐射X射线断层扫描成像技术(SXCT)和其他非原位测试技术及有限元模拟,深入研究了LiNi0.8Co0.1Mn0.1O2|Li6PS5Cl|Li全固态电池的衰退机制.研究发现正极电化学-机械力学耦合失效诱导的反应异质性产生不均匀的锂... 详细信息

本文通过联用无损三维同步辐射X射线断层扫描成像技术(SXCT)和其他非原位测试技术及有限元模拟,深入研究了LiNi0.8Co0.1Mn0.1O2|Li6PS5Cl|Li全固态电池的衰退机制.研究发现正极电化学-机械力学耦合失效诱导的反应异质性产生不均匀的锂离子通量并传输到负极,进而导致不均匀的锂沉积、溶解行为及死锂的产生等.锂负极不均匀的电化学反应行为又反作用于正极并强化其反应异质性,形成一种正负极衰退互相促进的正强化机制.随着电池继续循环,正负极的不均匀反应加剧造成其结构破坏,同时正负极体积缩胀引起电解质巨大塑性变形,最终致使电池失效.对比实验表明采用LiZr_(2)(PO_(4))_(3)(LZP)对正极进行改性,不仅有效抑制了正极的电化学-机械力学耦合失效,而且显著提高了负极锂沉积-溶解均匀性和电解质的结构完整性.该研究首次提出了全固态金属锂电池正负极相互依赖、相互关联的失效机制,为提升全固态金属锂电池性能提供了新思路.

关键词： Current density distribution Lithium ion flux Solid-state lithium metal batteries Codependent failure mechanism Cathode deactivation

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：