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放射学实践 1989年第1期4卷 21-23页

作者： Von g.wolf 安汉祥 Rntgenstation der I.Universittsfrauenklinik Wien 同济医科大学附属同济医院

藉助乳房X 线照像术诊断隐匿性乳癌,使乳癌转移率明显下降。乳癌术前定位诊断在多数病例是必不可少的。

关键词：小乳癌隐匿性乳癌 X线诊断

艾滋病血清学诊断新方法——免疫斑点法的建立

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病毒学报 1990年第3期6卷 250-255页

作者：邵一鸣曾毅韩孟杰 wolf h 中国预防医学科学院病毒学研究所西德慕尼黑大学Pettenkofer研究所

运用基因重组技术在大肠杆菌生产出有诊断意义的人免疫缺损病毒(hIV)蛋白,将其初步提纯,再用快速蛋白液相层析技术进一步纯化,即可直接点样至硝酸纤维膜,用来检测hIV抗体。实验显示,这种免疫斑点法(Immunodot assay,IDA)的敏感性与间接... 详细信息

运用基因重组技术在大肠杆菌生产出有诊断意义的人免疫缺损病毒(hIV)蛋白,将其初步提纯,再用快速蛋白液相层析技术进一步纯化,即可直接点样至硝酸纤维膜,用来检测hIV抗体。实验显示,这种免疫斑点法(Immunodot assay,IDA)的敏感性与间接免疫荧光法(IIF)相同,而特异性比它高,与蛋白印迹法(WB)相近。由于IDA试剂成本比IIF低,可用于hIV抗体检测的初筛。与IIF相比,IDA法具有假阳性率低、试剂稳定性高、操作简便和不需要昂贵的荧光显微镜等优点。因而是一种适合在基层应用的方法。

关键词：艾滋病 hIV 免疫斑点法

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Self-portraits

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hallux rigidus: Joint preserving alternatives to arthrodesis- a review of the literature

作者： Ernst Rebel Norbert wolf (ed.)

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World Journal of Orthopedics 2014年第1期5卷 6-13页

作者： hans Polzer Sigmund Polzer Mareen Brumann wolf Mutschler Markus Regauer Munich University hospital Foot and Ankle Surgery Department of Trauma Surgery-Campus Innenstadt Department of hand Ellbow and Footsurgery ATOS Clinic Heidelberg

hallux rigidus describes the osteoarthritis of the firstmetatarsophalangeal joint. It was first mentioned in 1887. Since then a multitude of terms have been introduced referring to the same disease. The main complaints are pain especially during movement and a limited range of motion. Radiographically the typical signs of osteoarthritis can be observed starting at the dorsal portion of the joint. Numerous classifications make the comparison of the different studies difficult. If non-operative treatment fails to resolve the symptoms operative treatment is indicated. The most studied procedure with reproducible results is the arthrodesis. Nevertheless, many patients refuse this treatment option, favouring a procedure preserving motion. Different motion preserving and joint sacrificing operations such as arthroplasty are available. In this review we focus on motion and joint preserving procedures. Numer-ous joint preserving osteotomies have been described. Most of them try to relocate the viable plantar cartilage more dorsally, to decompress the joint and to increase dorsiflexion of the first metatarsal bone. Multiple studies are available investigating these procedures. Most of them suffer from low quality, short follow up and small patient numbers. Consequently the grade of recommendation is low. Nonetheless, joint preserving procedures are appealing because if they fail to relief the symptoms an arthrodesis or arthroplasty can still be performed thereafter.

关键词： hallux rigidus Osteoarthritis First metatarsophalangeal joint Joint preserving Operative treatment Osteotomy

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Brucke

Brucke

Value of three-dimensional reconstructions in pancreatic carcinoma using multidetector CT:Initial results

作者： Ulrike Lorenz Norbert wolf ed.

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World Journal of gastroenterology 2009年第46期15卷 5827-5832页

作者： Miriam Klauβ Max Schbinger Ivo wolf Jens Werner hans-Peter Meinzer hans-Ulrich Kauczor Lars grenacher Department of Diagnostic and Interventional Radiology University of Heidelberg Department of Medical and Biological Informatics German Cancer Research Center(DKFZ) Surgical Clinic University of Heidelberg

AIM:To evaluate the use of three-dimensional imaging of pancreatic carcinoma using multidetector computed tomography(CT)in a prospective ***:Ten patients with suspected pancreatic tumors were examined prospectively using multidetec-tor CT(Somatom Sensation 16,Siemens,Erlangen,germany).The images were evaluated for the pres-ence of a pancreatic carcinoma and invasion of the peripancreatic vessels and surrounding *** the isotropic CT data sets,a three-dimensional image was created with automatic vascular analysis and semi-automatic segmentation of the organs and pancreatic tumor by a *** CT examinations and the three-dimensional images were presented to the sur-geon directly before and during the patient's operation using the Medical Imaging Interaction Toolkit-based software "ReLiver".Immediately after surgery,the value of the two images was judged by the *** operation and the histological results served as the gold ***:Nine patients had a pancreatic carcinoma(all pT3),and one patient had a serous *** tumor inf iltrated the superior mesenteric *** inf iltration was correctly *** carcinomas were *** comparison to the CT image with axial and coronal reconstructions,the three-dimensional image was judged by the surgeons as better for operation planning and consistently described as ***:A 3D-image of the pancreas repre-sents an invaluable aid to the ***,the 3D-software must be further developed in order to be integrated into daily clinical routine.

关键词： Pancreatic carcinoma 3D-reconstruction Multidetector computed tomography Pancreatic carcinomainvasion Segmentation

COmbination of Targeted temperature management and Thrombectomy after acute Ischemic Stroke(COTTIS):a pilot study

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Stroke & Vascular Neurology 2024年第3期9卷 258-267页

作者： Jürgen Bardutzky Rainer Kollmar Forat Al-Rawi Johann Lambeck Mohammad Fazel Christian Taschner wolf-Dirk Niesen Department of Neurology and Neurophysiology Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburgGermany Neurology and Neurointensive Care Darmstadt HospitalDarmstadtGermany BrainCool AB LundSweden Department of Neuroradiology Medical Center-University of FreiburgFaculty of MedicineUniversity of FreiburgFreiburgGermany

Background To evaluate the feasibility and safety of a fast initiation of cooling to a target temperature of 35℃by means of transnasal cooling in patients with anterior circulation large vessel occlusion(LVO)undergoing endovascular thrombectomy(EVT).Methods Patients with an LVO onset ofhour who had an indication for EVT were included in the *** cooling(RhinoChill)was initiated immediately after the patient was intubated for EVT and continued until an oesophageal target temperature of 35℃was *** was maintained with surface cooling for 6-hour postrecanalisation,followed by active rewarming(+0.2℃/hour).The primary outcome was defined as the time required to reach 35℃,while secondary outcomes comprised clinical,radiological and safety *** Twenty-two patients(median age,77 years)were included in the study(14 received additional thrombolysis,4 additional stenting of the proximal internal carotid artery).The median time intervals were 309min for last-seen-normal-to-groin,58min for door-to-cooling-initiation,65min for door-to-groin and 123min for *** target temperature of 35℃was reached within 30min(range 13–78min),corresponding to a cooling rate of 2.6℃/*** recanalisation,86%of the patients had a body temperature of≤35℃.The median National Institutes of health Stroke Scale at admission was 15 and improved to 2 by day 7,and 68%of patients had a good outcome(modified Rankin Scale 0–2)at 3 *** complications included asymptomatic bradycardia(32%),pneumonia(18%)and asymptomatic haemorrhagic transformation(18%).Conclusion The combined application of hypothermia and thrombectomy was found to be feasible in sedated and ventilated *** events were comparable to those previously described for EVT in the absence of *** effect of this procedure will next be evaluated in the randomised COmbination of Targeted temperature management and Thrombectomy after acute Ischemic Stro

关键词： maintained continued initiated

Estimate of oxygen consumption and intracellular zinc concentration of human spermatozoa in relation to motility

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Asian Journal of Andrology 2003年第1期5卷 3-8页

作者： Ralf R.henkel Kerstin Defosse hans-Wilhelm Koyro Norbert Weissmann wolf-Bernhard Schill Center for Dermatology and Andrology University D-35385 Giessen GermanyCenter for Dermatology and Andrology University D-35385 Giessen GermanyInstitute for Plant Ecology University D-35385 Giessen GermanyCenter for Internal Medicine Justus Liebig University D-35385 Giessen GermanyCenter for Dermatology and Andrology University D-35385 Giessen Germany

Aim: To investigate the human sperm oxygen/energy consumption and zinc content in relation to motility. Methods: In washed spermatozoa from 67 ejaculates, the oxygen consumption was determined. Following calculation of the total oxygen consumed by the Ideal gas Law, the energy consumption of spermatozoa was calculated. In addition, the zinc content of the sperm was determined using an atomic absorption spectrometer. The resulting data were correlated to the vitality and motility. Results: The oxygen consumption averaged 0.24μmol/106 sperm×24 h, 0.28μmol/106 live sperm×24 h and 0.85μmol/106 live & motile sperm×24 h. Further calculations revealed that sperm motility was the most energy consuming process (164.31 mJ/106 motile spermatozoa×24 h), while the oxygen consumption of the total spermatozoa was 46.06 mJ/106 spermatozoa×24 h. The correlation of the oxygen/ energy consumption and zinc content with motility showed significant negative correlations (r= -0.759; Phowever, when correlating sperm energy consumption with the zinc content, a significant positive relation (r=0.323; P=0.01) was observed. Conclusion: Poorly motile sperm are actually wasting the available energy. Moreover, our data clearly support the 'geometric Clutch Model' of the axoneme function and demonstrate the importance of the outer dense fibers for the generation of sperm motility, especially progressive motility.

关键词： oxygen energy consumption human spermatozoa sperm motility zinc

A gIS tool for infinite slope stability analysis(gIS-TISSA)

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geoscience Frontiers 2021年第2期12卷 756-768页

作者： Rüdiger Escobar-wolf Jonathon D.Sanders C.L.Vishnu Thomas Oommen K.S.Sajinkumar Department of geological and Mining Engineering and Sciences Michigan Technological UniversityHoughtonMIUSA Department of geology University of KeralaThiruvananthapuram 695581KeralaIndia

Landslides are one of the most common and a destructive natural hazard in mountainous terrain and thus evaluating their potential locations and the conditions under which they may occur is crucial for their hazard *** landslide occurrence in soil and regolith covered slopes are often modeled using the infinite slope model,which characterizes the slope stability in terms of a factor of safety(FS) *** approaches have been followed to also assess and propagate uncertainty through such ***(2004) introduced the use of the First Order Second Moment(FOSM) method to propagate input uncertainty through the infinite slope model,further developing the model and implementing it in the PISA-m software package(haneberg,2007).here we present an ArcPy implementation of PISA-m algorithms,which can be run from ESRI ArcMap in an entirely consistent georeferenced framework,and which we call "gIS Tool for Infinite Slope Stability Analysis"(gIS-TISSA).Users can select between different input options,e.g.,following a similar input style as for PISA-m,i.e., using an *** parameters input file,or providing each input parameter as a raster or constant value,through the program graphic user *** outputs can include FS mean and standard deviation estimates,the probability of failure(FS he same seismic analysis approach as in PISA-m, the Newmark acceleration can also be done,for which raster files of the mean,standard deviation,probability of exceedance,and RI are also *** of the code is done by replicating the results obtained with the PISA-m code for different input options,within a 10-5 relative *** Carlo modeling is also applied to validate gIS-TISSA outputs,showing a good overall correspondence.A case study was performed for Kannur district,Kerala,India,where an extensive landslide databa se for the year 2018 was available.81.19% of the actual

关键词： Landslides gIS-TISSA PISA-m FOSM Factor of Safety

Epstein-Barr病毒BLRF2基因重组质粒的构建及其在真核细胞中的表达

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病毒学报 1997年第1期13卷 75-78页

作者：王汉明周玲张晓梅曾毅 h wolf 中国预防医学科学院病毒学研究所

Ｅｐｓｔｅｉｎ－Ｂａｒ病毒ＢＬＲＦ２基因重组质粒的构建及其在真核细胞中的表达王汉明周玲张晓梅曾毅ＨＷｏｌｆ（中国预防医学科学院病毒学研究所，北京１０００５２）关键词Ｅｐｓｔｅｉｎ－Ｂａｒ病毒，ｐＨＤ－ＢＬＲＦ２，短... 详细信息

Ｅｐｓｔｅｉｎ－Ｂａｒ病毒ＢＬＲＦ２基因重组质粒的构建及其在真核细胞中的表达王汉明周玲张晓梅曾毅ＨＷｏｌｆ（中国预防医学科学院病毒学研究所，北京１０００５２）关键词Ｅｐｓｔｅｉｎ－Ｂａｒ病毒，ｐＨＤ－ＢＬＲＦ２，短暂表达，Ｐ２３，鼻咽癌Ｅｐｓｔｅｉ...

关键词： EV病毒 phD-BLRF2 表达基因重组质粒鼻咽癌