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Fetal vs adult mesenchymal stem cells achieve greater gene expression, but less osteoinduction

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World Journal of Stem Cells 2015年第1期7卷 223-234页

作者： Juan e Santiago-Torres Rebecca lovasz Alicia l Bertone Comparative Orthopedic Research laboratory Department of Veterinary Clinical Sciences College of Veterinary Medicine The Ohio State University Columbus OH 43210 United States Col.e.e of Medicine The Ohio State University Columbus OH 43210 United States

AIM: To investigate adenoviral transduction in mesenchymal stem cells(MSCs) and effects on stemness in vitro and function as a cell therapy in ***: Bone marrow-derived adult and fetal MSC were isolated from an equine source and expanded in monolayer tissue culture. Pol.e.hylenimine(PeI)-mediated transfection of pc DNA3-e GFP or adenoviral transduction of green fluorescent protein(GFP) was evaluated in fetal MSCs. Adenoviral-mediated transduction was chosen for subsequent experiments. All.e.periments were carried out at least in triplicate unless otherwise noted. Outcome assessment was obtained by flow cytometry or immunohystochemistry and included transduction efficiency, cell viability, stemness(i.e., cell proliferation, osteogenic and chondrogenic cell differentiation), and quantification of GFP expression. Fetal and adult MSCs were then transduced with an adenoviral vector containing the gene for the bone morphogenic protein 2(BMP2). In vitro BMP2 expression was assessed by enzyme linked immunosorbent assay. In addition, MSC-mediated gene delivery of BMP2 was evaluated in vivo in an osteoinduction nude mouse quadriceps model. New bone formation was evaluated by microradiography and ***: PeI provided greater transfection and viability in fetal MSCs than other commercial chemical reagents. Adenoviral transduction efficiency was superior to PeI-mediated transfection of GFP in fetal MSCs(81.3% ± 1.3% vs 35.0% ± 1.6%, P lar in adult MSCs(78.1% ± 1.9%). Adenoviral transduction provided significantly greater expression of GFP in fetal than adult MSCs(7.4 ± 0.1 vs 4.4 ± 0.3 millions of mean fluorescence intensity units, P ell as significantly greater in vitro BMP2 expression(0.16 pg/cell-day vs 0.10 pg/cell-day, P etal MSC GFP positive cells decreased significantly faster than adult MSCs(1.15% ± 0.05% vs 11.4% ± 2.1% GFP positive at 2 wk post-transduction, P ell proliferation and osteo

关键词： Mesenchymal stem cells Biomarkers Next generation sequencing Diagnostic tool Tumor-initiating cells

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地热钻探常用技术(上)

钻探工程 1981年第4期 56-58页

作者： l.e.卡帕诺 ,耿俊峰

对地热钻井进行广泛深入的研究，必须对所有各种类型的热储的不同钻进技术工艺的现状进行一次回顾。本文所提供的材料将对以下一些主题进行讨论：钻进前应考虑的各种因素，钻井程序细节、钻井液、水泥固井、井口装置、防喷器的配置以及... 详细信息

对地热钻井进行广泛深入的研究，必须对所有各种类型的热储的不同钻进技术工艺的现状进行一次回顾。本文所提供的材料将对以下一些主题进行讨论：钻进前应考虑的各种因素，钻井程序细节、钻井液、水泥固井、井口装置、防喷器的配置以及在地热钻井中逐渐推广的某些应特殊考虑的内容等等。为了清楚地讲述钻进操作与后勤支援工作，本文对气控热储和液控热储将分别进行讨论。

关键词：地热钻探固井水泥地热钻井热储储热层地热井热水井

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地热钻探常用技术(中)

钻探工程 1981年第5期 65-66页

作者： l.e.卡帕诺 ,耿俊峰

中间套管阶段直径13（3/8）英寸的中间套管，下在17（1/2）英寸的钻孔内。13（3/8）英寸的套管柱的设计方法与石油操作中的非常相似。确定了安全系数，除了温度的作用之外，所开列的设计依据都和油田应用中一样。从地表到1900英尺，13（... 详细信息

中间套管阶段直径13（3/8）英寸的中间套管，下在17（1/2）英寸的钻孔内。13（3/8）英寸的套管柱的设计方法与石油操作中的非常相似。确定了安全系数，除了温度的作用之外，所开列的设计依据都和油田应用中一样。从地表到1900英尺，13（3/8）英寸API K-55级、54.5磅/英尺的套管是令人满意的。此外，更重的套管也需要，通常是61磅/英尺，从1900英尺下到大约2800英尺处。据估计有H2S存在时，要使用较软的等级。

关键词：套管柱水泥衬管空气钻进定向钻进螺杆钻循环介质循环悬浮液回接套管地层水地下水钻具

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美国侵权法:产品责任发展的新动向

中外法学 1984年第1期 32-34页

作者： l.e.格雷利克王卫国

不久前,在美国的加利福尼亚州,要求追究十一家药品制造商的产品责任的诉讼,使因果关系问题再次成为争论的焦点。在辛德尔诉艾博特制药厂案(加利福尼亚州,1980年)中,争论的焦点不但没有脱离原告人所受的损害,而且可以说仍然是围绕着作为... 详细信息

不久前,在美国的加利福尼亚州,要求追究十一家药品制造商的产品责任的诉讼,使因果关系问题再次成为争论的焦点。在辛德尔诉艾博特制药厂案(加利福尼亚州,1980年)中,争论的焦点不但没有脱离原告人所受的损害,而且可以说仍然是围绕着作为"实际原因"的特定被告人的认定问题。加利福尼亚最高法院在一个四票对三票的判决中,把因果关系与

关键词：市场份额责任制造商 DeS 原告人被告人美国美利坚合众国北美洲

A platform for high-repetition-rate laser experiments on the large Plasma Device

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High Power laser Science and engineering 2018年第2期6卷 32-37页

作者： D.B.Schaeffer l.R.Hofer e.N.Knall.e.V.Heuer C.G.Constantin C.Niemann Department of Physics and Astronomy University of California-Los AngelesLos AngelesCA 90095USA

We present a new experimental platform for studying laboratory astrophysics that combines a high-intensity, highrepetition-rate laser with the large Plasma Device at the University of California, los Angeles. To demonstrate the utility of this platform, we show the first results of volumetric, highly repeatable magnetic field and electrostatic potential measurements, along with derived quantities of electric field, charge density and current density, of the interaction between a super-Alfv′enic laser-produced plasma and an ambient, magnetized plasma.

关键词： high energy density physics plasmas astrophysics

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Analysis of statistical dependencies between intel.e.tual characteristics and gaming ability,measured with the aid of a computer game

作者： e.l.·伏尼契 e.l·Voynich

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International Journal of Modeling, Simulation, and Scientific Computing 2020年第6期11卷 23-44页

作者： A.A.Margolis l.S.Kuravsky S.l.Artemenkov V.K.Voitov e.A.Shepeleva e.V.Gavrilova S.S.ermakov Moscow State University of Psychology and education Moscow Russia

The aim of this paper is testing a computer game as an al.e.native tool for the assessment of intelligence and creativity of school *** relevance of the research task is determined by the growing interest in practical psychology in the use of computer games as a special diagnostic tool in educational *** are two original factor models that have been developed and the structural.e.uation modeling of measured parameters has been carried *** relationships between game dynamic and final individual results in the computer game,on the one hand,and psychometric intelligence and divergent creativity,on the other,are *** correlation between the above-mentioned and individually measured intel.e.tual and creativity characteristics of the participants with the computer game results was *** of these characteristics in the form of a common latent factor makes it possible to identify the corresponding dependencies only in some cases when there is a dividing the sample into groups according to their ability ***,the dynamics of game scoring allows to refer participants to the target group with a certain level of abilities(high intelligence and creativity versus low intelligence and creativity),and it is the high level of psychological traits that determines effectiveness in the game behavior,but not vice *** qualitative ratios of the two groups of subsets of participants were revealed:weak,medium and strong players and players with three different levels of psychological *** prospects for the further use of computer games for assessment of cognitive abilities in the measurement of combination of psychological abilities are discussed.

关键词： Structural.e.uation modeling intel.e.tual characteristics gaming ability psychological diagnostics

Current and future perspectives on acute-on-chronic liver failure: Chal.e.ges of transplantation, machine perfusion, and beyond

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World Journal of Gastroenterology 2022年第48期28卷 6922-6934页

作者： Bianca Della Guardia Amanda P C S Boteon Celso e l Matielo Guil.e.me Felga Yuri l Boteon liver Unit Hospital Israelita Albert EinsteinSão Paulo 05652-900Brazil Instituto Israelita de ensino e Pesquisa Al.e.t einstein Faculdade Israelita de Ciências da Saúde Albert EinsteinSão Paulo 05652-900Brazil

Acute-on-chronic liver failure(AClF)is a syndrome that occurs in patients with chronic liver disease and is characterized by acute decompensation,organ failure and high short-term *** due to the lack of universal diagnostic criteria,the actual AClF prevalence remains unclear;nevertheless,it is expected to be a highly prevalent condition *** transplantation is an effective protective measure for selected AClF *** liver transplantation,diagnosing and treating precipitant events and providing supportive treatment for organ failures are currently the cornerstone of AClF *** new clinical specific therapies have been researched,more studies are necessary to assess safety and ***,future AClF management strategies must consider measures to improve access to liver transplantation because the time window for this life-saving therapy is frequently ***,an urgent and global discussion about allocation and prioritization for transplantation in critically ill AClF patients is needed because there is evidence suggesting that the current model may not portray their waitlist *** addition,while donor organ quality is meant to be a prognostic factor in the AClF setting,recent evidence suggests that machine perfusion of the liver may be a safe tool to improve the donor organ pool and expedite liver transplantation in this scenario.

关键词： Acute-on-chronic liver failure liver cirrhosis liver transplantation Machine perfusion Hypothermic oxygenated machine perfusion

Relationship between single and bulk mechanical properties for zeolite ZSM5 spray-dried particles

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Particuology 2014年第3期12卷 130-138页

作者： M.Marigo D.l.Cairns J.Bowen A.Ingram e.H.Stitt Johnson Matthey Technology Centre P.O.Box 1Belasis AvenueBillinghamCleveland TS23 1LBUnited Kingdom Centre for Formulation engineering Chemical EngineeringSchool of EngineeringThe University of BirminghamB15 2TTUnited Kingdom

In this work typical mechanical properties for a catalyst support material, ZSM5 （a spray-dried granular zeolite）, have been measured in order to relate the bulk behaviour of the powder material to the single particle mechanical properties. Particle shape and size distribution of the powders, determined by laser diffraction and scanning electron microscopy （SeM）, confirmed the spherical shape of the spray-dried particles. The excel.e.t flowability of the material was assessed by typical methods such as the Hausner ratio and the Cart index, This was confirmed by bulk measurements of the particle-particle internal friction parameter and flow function using a Schul.e.shear cell, which also illustrated the low compressibility of the material. Single particle compression was used to characterize single particle mechanical properties such as reduced elastic modulus and strength from Hertz contact mechanics theory. Comparison with surface properties obtained from nanoindentation suggests heterogeneity, the surface being harder than the core. In order to evaluate the relationship between single particle mechanical properties and bulk compression behaviour, uniaxial confined compression was carried out. It was determined that the Adams model was suitable for describing the bulk compression and furthermore that the Adams model parameter, apparent strength of single particles, was in good agreement with the single particle strength determined from single particle compression test.

关键词： Zeolite particle Flowability Powder flow function effective angle of internal friction Schul.e.shear cell Nanoindentation Single particle compression Bulk compression

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