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Helicobacter pylori Infection Facilitates the Expression of Resistin-like Molecule Beta in Gastric Carcinoma and Precursor Lesions

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Current Medical Science 2020年第1期40卷 95-103页

作者： Hong-jun LI er-hu FAnG Jian-qun WAnG Li-duan ZHEnG Qiang-song TOnG Department of Pathology Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China Department of Surgery Union HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China

Helicobacter pylori(***)was reported to be associated with gastric ***-like molecule beta(RELMβ),a recently described goblet cell-specific protein,was demonstrated to aberrantly express in gastric cancer and correlated with its clinicopathological *** study aimed to examine the association between *** and RELMp expression in gastric carcinoma and precursor *** infection and RELMβexpression were immunohistochemically evaluated in gastric biopsies from 230 *** biopsies consisted of normal gastric mucosa(w=20),mucosa with chronic gastritis(n=41),intestinal metaplasia(n=42),dysplasia(w=31),intestinal-type adenocarcinoma(n=56),and diffuse-type adenocarcinoma(n=40).RELMβ expression was measured in gastric biopsies after *** eradication therapy in a subgroup of 32 *** gastric cancer cell line SGC-7901 was infected with *** strains,and RELMp expression was detected by reverse transcription PCR,real-time PCR and Western *** RELMP immunoreactivity was observed in *** intestinal metaplasia(P=0.003),dysplasia(P=0.032),intestinal-type(P=0.037)and diffuse-type adenocarcinomas(P=0.001)than in ***-negative *** rates of RELMβ in dysplasia(CO.005),intestinal-type adenocarcinoma(Pnd diffuse-type adenocarcinoma(P=0.001)were significantly correlated with the grade of *** *** addition,*** eradication reduced the RELMβintensity in intestinal metaplasia(P=0.001).Infection of gastric cancer SGC-7901 cells with cag pathogenicity island(PAI)-positive *** Tn2,but not with its PAI totally deleted mutant(Tn2-APAI)for 4-8 h,resulted in enhanced protein and transcript levels of RELMβ(Pn summary,our study suggested that *** infection facilitated the expression of RELMβ in gastric garcinoma and precursor lesions.

关键词： resistin-like molecule beta Helicobacter pylori gastric carcinoma immunohistochemistry

基于MaxEnt模型的芦苇中国潜在分布区预测

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湖南农业科学 2024年第8期 66-72页

作者：王二国游澜清易自力杜卫红湖南农业大学生物科学技术学院湖南长沙410128 国家能源非粮生物质原料研发中心湖南分中心湖南长沙410128

研究基于338条芦苇分布数据和6个环境变量,利用MaxEnt模型对芦苇在我国的潜在分布区进行模拟,结合环境变量的贡献率、置换重要性、刀切法检验结果以及单变量响应曲线综合分析影响芦苇分布的主要环境变量及其适宜范围。结果表明:MaxEnt... 详细信息

研究基于338条芦苇分布数据和6个环境变量,利用MaxEnt模型对芦苇在我国的潜在分布区进行模拟,结合环境变量的贡献率、置换重要性、刀切法检验结果以及单变量响应曲线综合分析影响芦苇分布的主要环境变量及其适宜范围。结果表明:MaxEnt模型在RM=1、FC=LQHP组合参数下的AUC均值为0.909,表明预测结果准确度极高;芦苇在我国的潜在分布区主要位于甘肃、山西、陕西等地区,其中高、中适生区面积分别为1.25×10^(9)、1.88×10^(9) hm^(2);影响芦苇分布的主要环境变量是年平均气温、最湿月降水量、等温性、最冷季度降水量和年平均气温变化范围;当年平均气温为14~22℃、最湿月降水量为108~284 mm、等温性低于24、最冷季度降水量小于50 mm、年平均气温变化范围低于28.5℃时,芦苇的分布概率较高。

关键词：芦苇 MaxEnt模型环境因子潜在适生区

经皮微球囊压迫术和射频热凝术治疗原发性三叉神经痛的有效性和安全性研究

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中国疼痛医学杂志 2024年第3期30卷 183-188页

作者：张媛婧王稳刘春华努尔比亚·阿布拉李怡帆樊碧发北京中医药大学研究生院北京100029 中日友好医院疼痛科北京100029

目的:比较经皮微球囊压迫术和射频热凝术治疗原发性三叉神经痛(primary trigeminal neuralgia,PTn)的疗效及安全性。方法:回顾性分析2020年11月至2022年3月中日友好医院疼痛科收治的PTn病人,根据手术方式不同分为经皮球囊压迫组(P组)55... 详细信息

目的:比较经皮微球囊压迫术和射频热凝术治疗原发性三叉神经痛(primary trigeminal neuralgia,PTn)的疗效及安全性。方法:回顾性分析2020年11月至2022年3月中日友好医院疼痛科收治的PTn病人,根据手术方式不同分为经皮球囊压迫组(P组)55例和射频热凝组(R组)53例。观察两组术前、术后1小时的视觉模拟评分法(visual analogue scale,VAS)评分、总体疗效、并发症发生情况,随访两组术后1个月、3个月、6个月、1年的疼痛复发率。结果:两组病人术前VAS评分差异无统计学意义,术后1小时VAS评分明显低于术前(Pn,均可出现面部麻木等并发症,1年后射频热凝组的复发率更高。

关键词：原发性三叉神经痛经皮球囊压迫术射频热凝术疗效并发症

How we can improve patients’comfort after Milligan- Morgan open haemorrhoidectomy

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World Journal of Gastroenterology 2011年第11期17卷 1448-1456页

作者： Ma-Mu-Ti-Jiang A ba-bai-ke-re Hong-Guo Huang Wen-ni Re Kai Fan Hui Chu er-Ha-Ti Ai Mai-Mai-Ti-Tu-er-Xun KE Li-Mu Yi-Rui Wang Hao Wen Department of Anorectal Surgical First Affiliated Hospital of Xinjiang Medical University Urumqi 830011 Xinjiang Uygur Autonomous Region China Department of Otolaryngeological Urumqi General Hospital Lanzhou Military Command Urumqi 830011 Xinjiang Uygur Autonomous Region China Department of Pharmocological First Affiliated Hospital of Xinjiang Medi- cal University Ururnqi 830011 Xinjiang Uygur Autonomous Region China Digestive and Vascular Surgical Center First Affili. ated Hospital Xinjiang Medical University Urumqi 830011 Xinjiang Uygur Autonomous Region China

AIM:To demonstrate the value of Diosmin(flavonidic fraction)in the management of post-haemorhoidectomic ***:Eighty-six consecutive patients with grades ⅢandⅣacute mixed hemorrhoids admitted to the Anorectal Surgical Department of First Affiliated Hospital,Xinjiang Medical University from April 2009 to April 2010,were enrolled in this *** observerblinded,randomized trial was conducted to compare post-haemorhoidectomic symptoms with use of Diosmin flavonidic fraction vs ***-six patients were randomly allocated to receive Diosmin flavonidic fraction 500 mg for 1 wk(n=43)or placebo(n=43).The Milligan-Morgan open haemorrhoidectomy was performed by a standardized diathermy excision ***,bleeding,heaviness,pruritus,wound edema and mucosal discharge were observed after *** postoperative symptoms and hospitalization time were ***:The mean age of the Diosmin group and controls was 53.2 and 51.3 years,*** Diosmin group,haemorrhoid piles were of the third degree in 33 patients and the fourth degree in 10;and in the control group,29 were of the third degree and 14 were of the fourth *** was no statistically significance in age,gender distribution,degree and number of excised haemorrhoid piles,and the mean duration of haemorrhoidal disease between the two *** was a statistically significant improvement in pain,heaviness,bleeding,pruritus from baseline to the 8th week after operation(Pnts taking Diosmin had a shorter hospitalization stay after surgery(Pere was also a significant improvement on the proctoscopic appearance(Per,there was no statistical difference between the two groups in terms of wound mucosal *** patients experienced minor bleeding at the 8th week in Diosmin group,and underwent ***:Diosmin is effective in alleviating postoperational symptoms of ***,it should be considered for the initial treatment afte

关键词： Flavonidic fraction Postoperative complication Haemorrhoids

HDACi和RS-1提高CRISPR/Cas12i介导的HDR编辑效率

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农业生物技术学报 2024年第10期32卷 2306-2323页

作者：陈秋崇李尚朴苗洱钰周冰倩王旭孟祥宇王小龙徐坤西北农林科技大学动物科技学院/农业农村部动物生物育种国际联合研究中心/陕西省动物遗传育种与繁殖重点实验室

CRISPR/Cas12i是我国学者新近开发的、具有自主知识产权的CRISPR基因编辑系统，被证实具有不亚于CRISPR/Cas9系统的打靶效率。同源定向修复（homology-directed repair, HDR）是DnA双链断裂（double-stranded break, DSB）的主要修复方... 详细信息

CRISPR/Cas12i是我国学者新近开发的、具有自主知识产权的CRISPR基因编辑系统，被证实具有不亚于CRISPR/Cas9系统的打靶效率。同源定向修复（homology-directed repair, HDR）是DnA双链断裂（double-stranded break, DSB）的主要修复方式之一。基于HDR机制的基因编辑可用于纠正基因组中任何形式的突变，但受限于哺乳动物细胞中普遍较低的HDR效率。本研究首先通过单链复性（single-strand annealing, SSA）报告试验、剂量梯度和浓度梯度实验分别验证CRISPR/Cas12i系统在人（Homo sapiens）胚肾细胞系HEK293T中不同靶点的活性、介导HDR编辑的单链寡核苷酸（single-stranded oligonucleotides,ssODn）供体模板剂量及添加小分子药物的适宜浓度，进而通过流式细胞分选、基因组PCR、Sanger测序和在线预测等手段，检测添加不同小分子药物对HEK293T细胞和绵羊（Ovis aries）胎儿成纤维细胞中CRISPR/Cas12i系统介导的HDR编辑效率的影响。结果显示，CRISPR/Cas12i系统在HEK293T细胞18个不同靶点均表现出较高编辑活性，除2个位点稍低外，其余均在80%左右；不同剂量及长度的ss ODn对HDR效率有一定影响，且小分子药物适宜浓度在不同物种和不同类型细胞中略有不同；组蛋白去乙酰化酶抑制剂（histone deacetylase inhibitor, HDACi）和RS-1 (C20H16Br2n2O3S)对HEK293T细胞和绵羊胎儿成纤维细胞中CRISPR/Cas12i系统介导的HDR编辑效率均有显著提升，其中RS-1细胞毒性最小，且在提升HDR效率同时未显著降低插入/缺失突变（in.ertion/deletion mutation, InDel）效率；此外，Entinostat作为HDACi之一，在绵羊胎儿成纤维细胞的骨形态发生蛋白受体1B （bone morphogenetic protein receptor 1B,BMPR1B）基因相关位点提升HDR编辑效率约148倍。总之，CRISPR/Cas12i系统具有较高编辑活性，能够在模式细胞和绵羊原代细胞中介导有效的、以ssODn为供体的HDR精确编辑，且适宜浓度的HDACi和RS-1可以有效提高HDR编辑效率。本研究为CRISPR/Cas12i基因编辑系统的推广与应用提供了参考和借鉴。

关键词： CPRISPR/Cas12i 同源重组 RS-1 (C20H16Br2n2O3S) 组蛋白去乙酰化酶抑制剂(HDACi) 绵羊

滴灌施肥频率对烤烟生长、氮肥利用及烟叶产质量的影响

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节水灌溉 2024年第3期 68-74,82页

作者：马二登薛如君高天代快刘忠华李军营徐照丽邓小鹏赵正雄云南省烟草农业科学研究院昆明650031 云南省烟草公司大理州公司云南大理671000 海南红塔卷烟有限责任公司海口571137 云南省烟草公司玉溪市公司云南玉溪653100 中国烟草总公司云南省公司昆明650011 云南农业大学昆明650201

为探明滴灌施肥频率对烤烟生长、氮肥利用及烟叶产质量的影响,2018-2019年通过田间试验,以不施肥空白(CK)和当地常规处理(CF,即肥料干施+滴灌)为对照,研究低频滴灌施肥(DF1,即养分管理期1次滴灌施肥)、中频滴灌施肥(DF2,即养分管理期连... 详细信息

为探明滴灌施肥频率对烤烟生长、氮肥利用及烟叶产质量的影响,2018-2019年通过田间试验,以不施肥空白(CK)和当地常规处理(CF,即肥料干施+滴灌)为对照,研究低频滴灌施肥(DF1,即养分管理期1次滴灌施肥)、中频滴灌施肥(DF2,即养分管理期连续2天每天1次滴灌施肥)和高频滴灌施肥(DF3,即养分管理期连续3天每天1次滴灌施肥)对烤烟农艺性状、根系发育形态、氮肥累积量和利用效率及烟叶产质量的影响。结果表明,与CF相比,各滴灌施肥处理烟叶产量无显著差异(P<0.05),氮肥利用率有不同程度提高,烟株发育状况则因滴灌施肥频率而异。与DF1和DF2相比,DF3烟株旺长期叶面积系数分别增加了7.3%~33.3%和1.6%~28.7%,根系体积分别增加了3.9%~46.6%和21.2%~40.5%,氮素利用率分别提高了39.0%~95.0%和21.5%~34.3%,烟叶烟碱含量分别增加了17.5%~28.5%和12.5%~20.0%。与中频滴灌施肥和低频滴灌施肥相比,高频滴灌施肥有利于促进烟株生长和根系形态发育,提高氮肥利用效率和烟叶品质质量,是有效协同氮肥高效利用和烟叶产质量的滴灌施肥措施。

关键词：烤烟滴灌施肥频率氮肥利用根系形态

水氮处理对谷子内源激素脱落酸和光合作用特性的影响

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中国土壤与肥料 2024年第4期 190-198页

作者：于肖牛佳红陈二影秦岭杨延兵黎飞飞刘振宇管延安山东省农业科学院作物研究所/山东省特色作物工程实验室山东济南250100 山东师范大学生命科学学院山东济南250014

为探究氮肥与干旱处理对谷子生理特性的影响,以济谷22为材料,采用盆栽法研究不同水、氮处理对谷子内源激素脱落酸(ABA)、叶绿素含量、光合作用特性及水、氮利用效率的影响。设置3种水分处理:正常水分(CK,田间持水量的60%~70%)、苗期干旱... 详细信息

为探究氮肥与干旱处理对谷子生理特性的影响,以济谷22为材料,采用盆栽法研究不同水、氮处理对谷子内源激素脱落酸(ABA)、叶绿素含量、光合作用特性及水、氮利用效率的影响。设置3种水分处理:正常水分(CK,田间持水量的60%~70%)、苗期干旱(W_(1),田间持水量的30%~40%)、孕穗期干旱(W_(2),田间持水量的30%~40%)及2种施氮量处理:施氮(n_(150),n 150 kg/hm^(2))、不施氮(n_(0)),生长期间旱棚防雨。结果表明:受到水分胁迫时,叶片中的ABA含量升高,施氮水平下升高幅度较大;根中ABA含量也会升高,但其在不施氮水平下升高幅度较大。开花期不同处理光-光合响应曲线有显著差异,苗期干旱施氮处理优势明显。开花期叶片荧光性能存在较大差异,受到水、氮胁迫时,光系统Ⅱ潜在的最大光化学效率、反应中心所吸收的光子中用于电子传递的量子产额值降低,反应中心所吸收光子中用于热耗散的量子比率上升,以吸收光能为基础的性能指数急剧下降,孕穗期干旱施氮处理的j点的相对可变荧光强度显著高于对照,孕穗期干旱且不施氮的以吸收光能为基础的性能指数最低,反映单位反应中心捕获的用于电子传递的能量值最高。不同水、氮处理的谷子抽穗后,随着时间的推移,SPAD值逐渐降低,施氮处理明显提高了叶片的SPAD值。不同处理间比较以施氮处理下苗期干旱水分利用效率最高,不施氮处理的氮素利用效率明显高于其他处理。同一水分处理下,施氮处理的水分利用效率显著高于不施氮处理,氮素利用效率表现为相反趋势。苗期干旱施氮处理通过提高叶片SPAD值、花期净光合速率,显著提升了光合作用,水分和氮肥利用率显著提高,保证谷子达到相对稳定的产量。

关键词：谷子施氮处理水分胁迫脱落酸光合特性

Synthesis and characterization of novel low band-gap polymers containing squaraine units

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Chinese Chemical Letters 2007年第12期18卷 1531-1534页

作者： er Fu Huo Jiang Yong Wu Bao Feng Song Ying Li Qing Jiang Ming Gui Xie College of Chemistrk Sichuan University Chengdu 610064 China Beijing Research Institute of Chemical Industry SINOPEC Beijing 100013 China

novel conjugated polymers based on squaric acid having 2,5-Bis[（E）-n-alkylpyrrol-2-ylvinyl]-3-alkylthiophene （PVTVP） unit in the main chain were successfully synthesized in good yields through polycondensation reaction. Their molecular structures were characterized by PT-IR and IH nMR. They have good solubility in common organic solvents, good thermal stability by thermal gravimetric analysis and high molecular weights. Their optical properties were investigated by UV-vis absorption spectra in CH2C12 solution, the results indicated all these compounds showed broad and strong spectral responses from 200 nm to 900 nm, suggesting their potential for application as organic plastic solar cells.

关键词： Conjugated polymers Squarain Plastic solar cells

One in four patients with gastrointestinal bleeding develops shock or hemodynamic instability:A systematic review and meta-analysis

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World Journal of Gastroenterology 2023年第28期29卷 4466-4480页

作者： Mahmoud Obeidat Brigitta Teutsch Anett Rancz Edina Tari Katalin Márta Dániel Sándor Veres nóra Hosszúfalusi Emese Mihály Péter Hegyi Bálint erőss Centre for Translational Medicine Semmelweis UniversityBudapest 1085Hungary Institute for Translational Medicine Medical SchoolUniversity of PécsPécs 7623Hungary Department of in.ernal Medicine and Hematology Semmelweis UniversityFaculty of MedicineBudapest 1085Hungary Institute of Pancreatic Diseases Semmelweis UniversityBudapest 1083Hungary Department of Biophysics and Radiation Biology Semmelweis UniversityBudapest 1085Hungary

BACKGROUnD Hemodynamic instability and shock are associated with untoward outcomes in gastrointestinal ***,there are no studies in the existing literature on the proportion of patients who developed these outcomes after gastrointestinal *** To determine the pooled event rates in the available literature and specify them based on the bleeding *** The protocol was registered on PROSPerO in advance(CRD42021283258).A systematic search was performed in three databases(PubMed,EMBASE,and CEnTRAL)on 14^(th) October *** proportions with 95%CI were calculated with a random-effects model.A subgroup analysis was carried out based on the time of assessment(on admission or during hospital stay).Heterogeneity was assessed by Higgins and Thompson’s I^(2) *** Joanna Briggs Institute Prevalence Critical Appraisal Tool was used for the risk of bias *** Reference Citation Analysis(https://***/)tool was applied to obtain the latest highlight *** We identified 11589 records,of which 220 studies were eligible for data *** overall proportion of shock and hemodynamic instability in general gastrointestinal bleeding patients was 0.25(95%CI:0.17-0.36,I^(2)=100%).In non-variceal bleeding,the proportion was 0.22(95%CI:0.14-0.31,I^(2)=100%),whereas it was 0.25(95%CI:0.19-0.32,I^(2)=100%)in variceal *** proportion of patients with colonic diverticular bleeding who developed shock or hemodynamic instability was 0.12(95%CI:0.06-0.22,I^(2)=90%).The risk of bias was low,and heterogeneity was high in all *** One in five,one in four,and one in eight patients develops shock or hemodynamic instability on admission or during hospitalization in the case of non-variceal,variceal,and colonic diverticular bleeding,respectively.

关键词： Gastrointestinal bleeding Hemodynamic instability Shock Meta-analysis Statistics Review