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C-terminal domain of hepatitis C virus core protein is essential for secretion

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World Journal of Gastroenterology 2005年第25期11卷 3887-3892页

作者： Soo-ho choi Kyu-Jin Park So-Yeon Kim Dong-Hwa choi Jung-Min Park Soon B. Hwang Ilsong Institute of Life Science

AIM: We have previously demonstrated that hepatitis C virus (HCV) core protein is efficiently released into the culture medium in insect cells. The objective of this study is to characterize the HCV core secretion in insect cells. METhoDS: We constructed recombinant baculoviruses expressing various-length of mutant core proteins, expressed these proteins in insect cells, and examined core protein secretion in insect cells. RESULTS: Only wild type core was efficiently released into the culture medium, although the protein expression level of wild type core was lower than those of other mutant core proteins. We found that the shorter form of the core construct expressed the higher level of protein. however, if more than 18 amino acids of the core were truncated at the C-terminus, core proteins were no longer secreted into the culture medium. Membrane flotation data show that the secreted core proteins are associated with the cellular membrane protein, indicating that HCV core is secreted as a membrane complex. CONCLUSION: The C-terminal 18 amino acids of HCV core were crucial for core secretion into the culture media. Since HCV replication occurs on lipid raft membrane structure, these results suggest that HCV may utilize a unique core release mechanism to escape immune surveillance, thereby potentially representing the feature of HCV morphogenesis.

关键词： Hepatitis C virus Core secretion Morphogenesis Virus assembly

Predicting tumor response after preoperative chemoradiation using clinical parameters in rectal cancer

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World Journal of Gastroenterology 2011年第48期17卷 5310-5316页

作者： Chan ho Park Hee Cheol Kim Yong Beom Cho Seong Hyeon Yun Woo Yong Lee Young Suk Park Doo ho choi ho-Kyung Chun Departmentof Surgery Samsung Medical Center Sungkyunkwan Univer-sity School of Medicine Irwon-dong 50 Gangnam-gu Seoul135-710 South Korea Division of Hematology-Oncology Depart-ment of Medicine Samsung Medical Center Sungkyunkwan University School of Medicine Irwon-dong 50 Gangnam-guSeoul 135-710 South Korea Department of Radiation Oncology SamsungMedical Center Sungkyunkwan University School of MedicineIrwon-dong 50 Gangnam-gu Seoul 135-710 South Korea

AIM: To evaluate the clinical parameters and identify a better method of predicting pathological complete response (pCR). METhoDS: We enrolled 249 patients from a database of 544 consecutive rectal cancer patients who underwent surgical resection after preoperative chemoradiation therapy (PCRT). A retrospective review of morphological characteristics was then performed to collect data regarding rectal examination findings. A scoring model to predict pCR was then created. To validate the ability of the scoring model to predict complete ***: Seventy patients (12.9%) achieved a pCR. A multivariate analysis found that pre-CRT movability (P = 0.024), post-CRT size (P = 0.018), post-CRT morphology (P = 0.023), and gross change (P = 0.009) were independent predictors of pCR. The accuracy of the scoring model was 76.8% for predicting pCR with the threshold set at 4.5. In the validation set, the accuracy was 86.7%. CONCLUSION: Gross changes and morphological findings are important predictors of pathological response. Accordingly, PCRT response is best predicted by a combination of clinical, laboratory and metabolic information.

关键词： Rectal cancer Preoperative chemoradiotherapy Downstaging Tumor regression Validation

Rapid Preparation of Monolithic Columns for Capillary Electrochromatography Separation

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Chinese Chemical Letters 2006年第6期17卷 813-816页

作者： Wen Jun GONG Yi Jun ZHANG Yu Ping ZHANG Seong ho choi Henan Institute of Science and Technology Xinxiang 453003 Department of Chemistry Hannam University Daejon South Korea

Fritless packed silica gel columns were prepared using sol-gel technology, The part of a 75 μm i.d. capillary was filled with a mixture of methacryloxypropyltrimethoxysilane, toluene and hydrochloric acid. Four different photoinitiators such as benzoin methyl ether, Irgacure 819, Irgacure 1700 and Irgacure 1800 were added in the presence or absence of sodium dodecyl sulfate during the polymerization process. The above eight solutions were irradiated at 365 nm about 5-10 min to prepare the porous monolithic sol-gel columns by a one-step process.

关键词： Monolithic columns electrochromatography photopolymerized sol-gel.

Comparative long-term outcomes for pancreatic volume change, nutritional status, and incidence of new-onset diabetes between pancreatogastrostomy and pancreatojejunostomy after pancreaticoduodenectomy

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Hepatobiliary Surgery and Nutrition 2020年第3期9卷 284-295页

作者： Bong Jun Kwak ho Joong choi Young Kyoung You Dong Goo Kim Tae ho hong Department of Hepatobiliary and Pancreas Surgery Seoul St. Mary's Hospital College of Medicine The Catholic University of Korea Seoul Republic of Korea

Background:The difference in volume change in a pancreatic remnant according to the type of pancreaticoenterostomy after pancreaticoduodenectomy(PD)for long-term follow-up is ***,there are few studies that evaluate the difference in general nutritional status and pancreatic endocrine function,including new-onset diabetes mellitus(NODM)depending on the type of *** study aimed to compare serial pancreatic volume changes in pancreatic remnants between pancreatogastrostomy(PG)and pancreatojejunostomy(PJ)after PD and to evaluate the difference in general nutritional status and incidence of NODM between PG and ***:This study enrolled 115 patients who had survived for more than 3 years after *** were divided into the PG group and the PJ *** clinicopathologic factors were collected and *** calculated serial pancreas volume and pancreatic duct size precisely from preoperative stage to 5 years after surgery by image-processing software specifically designed for navigation and visualization of multimodality and multidimensional *** changes of albumin and body mass index(BMI)as related to general nutritional status were compared between the PG and PJ *** evaluate the incidence and risk factors of NODM following PD,subgroup analysis was performed in 88 patients who did not have diabetes ***:Most patient demographics were not significantly different between the PG group(n=45)and PJ group(n=70).There was no significant difference in volume reduction between the groups from postoperative 1 month to 5 years(PG group?18.21±14.66 mL versus PJ group?14.43±13.05 mL,P=0.209).But there was a significant difference in increased pancreatic duct size between the groups from postoperative 1 month to 5 years(PG group 1.66±2.20 mm versus PJ group 0.54±1.54 mm,P=0.007).There was no significant difference in the increase of total serum albumin between the groups for 5 years after surgery(PG group 0

关键词： Pancreaticojejunostomy pancreaticoduodenectomy(PD) nutritional status diabetes mellitus

Pravastatin activates PPARα/PPARγexpression in the liver and gallbladder epithelium of hamsters

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Hepatobiliary & Pancreatic Diseases International 2011年第2期10卷 185-190页

作者： Seok ho Dong Jin Lee Dong Hee Koh Min ho choi Hyun Joo Jang Sea Hyub Kae Division of Gastroenterology Department of Internal MedicineKyung Hee University College of Medicine Division of Gastroenterology Department of Internal MedicineHallym University College of Medicine

BACKGROUND:Our earlier study with cultured gallbladder epithelial cells demonstrated that statins(HMG-CoA reductase inhibitors)activate the expression of PPARαand PPARγ, consequently blocking the production of pro-inflmmatory *** present study used hamsters to investigate the effects of pavastatin on PPARα/PPARγexpression in the liver and gallbladder epithelium,and to determine whether pravastatin suppresses cholesterol crystal formation in the gallbladder. METhoDS:A total of 40 Golden Syrian male hamsters(4 weeks old)were randomly assigned to four groups(basal diet control; basal diet+pavastatin;high cholesterol diet;high cholesterol diet+pravastatin).All hamsters were 11 weeks old at the end of the *** liver,gallbladder and bile were harvested. Immunohistochemical staining and Western blotting for PPARαand PPARγwere performed in the liver and gallbladder. A drop of fresh bile was examined for cholesterol crystals under a microscope. RESULTS:In the gallbladder and liver of the hamsters, pravastatin activated the PPARαand PPARγexpression of gallbladder epithelial cells and hepatocytes,and particularly the response of PPARγwas much stronger than that of PPARα. Pravastatin suppressed the formation of cholesterol gallstones or crystals in the gallbladder. CONCLUSION:Pravastatin is an effective medication to activate PPARs(especially PPARγ)in the liver and the gallbladder epithelium of hamsters,and contributes to the prevention of gallstone formation.

关键词： pravastatin PPARα PPARγ hamster gallstone

Carcinoma of the middle bile duct:Is bile duct segmental resection appropriate?

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World Journal of Gastroenterology 2009年第47期15卷 5966-5971页

作者： Hyung Geun Lee Sang hoon Lee Dong Do Yoo Kwang Yeol Paik Jin Seok Heo Seong ho choi Dong Wook choi Department of Surgery Samsung Medical CenterSungkyunkwan University School of MedicineSeoul 135-710South Korea

AIM:To compare survival between bile duct segmental resection(BDSR)and pancreaticoduodenectomy(PD) for treating distal bile duct cancers. METhoDS:Retrospective analysis was conducted for 45 patients in a BDSR group and for 149 patients in a PD group. RESULTS:The T-stage(Phow any significant difference in survival when this was adjusted for the TNM *** 3-and 5-year survival rates were: stageⅠa[BDSR(100.0%and 100.0%)vs PD(76.9% and 68.4%)(P=0.226)];stageⅠb[BDSR(55.8% and 32.6%)vs PD(59.3%and 59.3%)(P=0.942)]; stageⅡb[BDSR(19.2%and 19.2%)vs PD(31.9%and 14.2%)(P=0.669)]. CONCLUSION:BDSR can be justified as an alternative radical operation for patients with middle bile duct inselected patients with no adjacent organ invasion and resection margin is negative.

关键词： Bile duct cancer Segmental resection Pancreaticoduodenectomy

Immunosuppressant treatment for IgG4-related sclerosing cholangitis: A case report

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World Journal of Clinical Cases 2021年第1期9卷 267-273页

作者： Jong-Sun Kim Won ho choi Kyung-Ann Lee Hyun-Sook Kim Department of Internal Medicine Soonchunhyang University Seoul HospitalSeoul 04401South Korea

BACKGROUND Immunoglobulin G4-related disease(IgG4-RD)is a multi-system fibroinflammatory disorder that can involve any organ,including the salivary glands,pancreas,and biliary *** of immunoglobulin G4-related sclerosing cholangitis(IgG4-SC)is similar to that for IgG4-RD,but progression is irreversible in some *** present a case of IgG4-SC in which an immuno-suppressant induced marked clinical and radiologic *** SUMMARY A 63-year-old male presented with a prominent itching sensation and wholebody *** showed obstructive-pattern jaundice,an elevated IgG4 level,and infiltration of a large number of IgG4-positive cells in the ampulla of *** imaging findings of intrahepatic duct(IHD)and common bile duct dilation,an elevated serum IgG4 level,and characteristic histological findings led to diagnosis of IgG4-SC that compatible with the 2019 ACR/EULAR classification *** planned to treat the patient with high-dose glucocorticoid(GC),followed by cyclophosphamide pulse *** treatment with high-dose GC and an immunosuppressant,imaging studies showed that IHD dilatation had completely *** Prompt diagnosis and appropriate treatment of IgG4-SC are *** there is a risk of relapse of IgG4-SC,the GC dose should be gradually reduced,and a maintenance immunosuppressant should be given.

关键词： Immunoglobulin G4-related disease Immunoglobulin G4-related sclerosing cholangitis Glucocorticoid Immunosuppressant Case report ACR/EULAR classification criteria

ELK3-ID4 axis governs the metastatic features of triple negative breast cancer

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Oncology Research 2024年第1期32卷 127-138页

作者： JIN-ho choi JOO DONG PARK SEUNG HEE choi EUN-SU KO HYE JUNG JANG KYUNG-SOON PARK Department of Biomedical Science CHA UniversitySeongnamKorea

Purpose:Cancer cell metastasis is a multistep process,and the mechanism underlying extravasation remains ***3 is a transcription factor that plays a crucial role in regulating various cellular processes,including cancer *** on the finding that ELK3 promotes the metastasis of triple-negative breast cancer(TNBC),we investigated whether ELK3 regulates the extravasation of TNBC by forming the ELK3-ID4 ***4 functions as a transcriptional regulator that interacts with other transcription factors,inhibiting their activity and subsequently influencing various biological processes associated with cell differentiation,survival,growth,and ***:We assessed the correlation between the expression of ELK3 and that of ID4 in TNBCs using bioinformatics analyses,QRT-PCR,western blot analysis,luciferase reporter assays,and chromatin ***,adhesion,invasion,and lung metastasis assays were employed to determine whether the ELK3-ID4 axis regulates the metastatic features of ***:We found that ELK3 binds directly to a binding motif close to the ID4 promoter to repress promoter *** expression of E-cadherin in TNBC was regulated by the ELK3-ID4 *** vitro and in vivo analyses showed that inhibiting ID4 expression in ELK3-knockdown MDA-MB-231(ELK3KD)cells restored the ability to extravasate and ***:The results indicate that the ELK3 regulates ID4 promoter activity,and that the ELK3-ID4 axis regulates the metastatic characteristics of TNBC ***,the data suggest that the ELK3-ID4 axis regulates metastasis of TNBCs by modulating expression of E-cadherin.

关键词： E-cadherin Extravasation Colonization

Clinical outcome in patients with hepatocellular carcinoma after living-donor liver transplantation

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World Journal of Gastroenterology 2013年第29期19卷 4737-4744页

作者： ho Joong choi Dong Goo Kim Gun Hyung Na Jae Hyun Han Tae ho hong Young Kyoung You Hepatobiliary-Pancreas Surgery and Liver Transplantation Division Department of Surgery Seoul St. Mary's Hospital The Catholic University of Korea Seoul 137-701 South Korea

AIM: To investigate risk factors for hepatocellular carcinoma (HCC) recurrence after living donor liver transplantation (LDLT) and efficacy of various criteria. METhoDS: From October 2000 to November 2011, 233 adult patients underwent LDLT for HCC at our institution. After excluding nine postoperative mortality cases, we analyzed retrospectively 224 patients. To identify risk factors for recurrence, we evaluated recurrence, disease-free survival (DFS) rate, survival rate, and various other factors which are based on the characteristics of both the patient and tumor. Additionally, we developed our own criteria based on our data. Next, we compared our selection criteria with various tumor-grading scales, such as the Milan criteria, University of California, San Francisco (UCSF) criteria, TNM stage, Barcelona Clinic Liver Cancer (BCLC) stage and Cancer of the Liver Italian Program (CLIP) scoring system. The median follow up was 68 (6-139) ***: In 224 patients who received LDLT for HCC, 37 (16.5%) experienced tumor recurrence during the follow-up period. The 5-year DFS and overall survival rates after LDLT in all patients with HCC were 80.9% and 76.4%, respectively. On multivariate analysis, the tumor diameter {5 cm; P howed a significant difference in DFS rate. And our criteria (P = 0.006) and UCSF criteria (P = 0.009) showed a significant difference in overall survival rate. Bu

关键词： Hepatocellular carcinoma Living donor liver transplantation Selection criteria Milan criteria University of California, San Francisco criteria Barcelona Clinic Liver Cancer Cancer of the Liver Italian Program