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Human leukocyte antigen compatibility and incidence of donorspecific antibodies in pediatric liver transplant recipients

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World Journal of Gastroenterology 2024年第33期30卷 3837-3845页

作者： Melina U Melere Flavia H Feier Jorge Neumann Antônio N Kalil Juliana de M Montagner Luiza s Nader Carolina s da silva Marco Aurélio F Junior Gabriela P Coral Guilherme P Bobsin Cristina T Ferreira Department of Hepatology and Liver Transplantation Santa Casa de Porto AlegrePorto Alegre 90050170Rio Grande do SulBrazil Department of Hepato-biliary-pancreatic surgery and Liver Transplantation Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil Postgraduation Program in Medicine:Hepatology Federal University of Health Sciences of Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Laboratory of Transplantation Immunology Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil

BACKGROUND Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DsAs)and their impact on graft *** substantial evidence for adult populations exists,research focusing on pediatric LT outcomes remains *** To investigate the prevalence of human leukocyte antigen(HLA)mismatches and DsA and evaluate their association with rejection episodes after pediatric *** A cohort of pediatric LT recipients underwent HLA testing at santa Casa de Porto Alegre,Brazil,between December 2013 and December *** patients who survived for>30 days after LT with at least one DsA analysis were *** classes I and II and cross-matches were *** presence of de novo DsA(dnDsA)was evaluated at least 3 months after LT using the Luminex®single antigen bead method,with a positive reaction threshold set at 1000 *** episodes were confirmed by liver *** Overall,67 transplanted children were analyzed;61 received grafts from living donors,85%of whom were related to ***-transplant DsA(class I or II)was detected in 28.3%of patients,and dnDsA was detected in 48.4%.The median time to DsA detection after LT was 19.7[interquartile range(IQR):4.3-35.6]*** rejection occurred in 13 patients at follow-up,with C4d positivity observed in 5/13 Liver *** median time to rejection was 7.8(IQR:5.7-12.8)*** presence of dnDsA was significantly associated with rejection(36%vs 3%,Psurvival rates at 12 and 24 months were 76%vs 100%and 58%vs 95%for patients with dnDsA anti-DQ vs those without,*** Our findings highlight the importance of incorporating DsA assessment into pre-and post-transplantation protocols for pediatric LT *** implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recip

关键词： Human leukocyte antigens Donor-specific antibodies Liver transplantation Pediatric Rejection

Correlation between Intestinal Health and Coccidiosis Prevalence in Broilers during Different seasons of the Year in Brazil from 2012 to 2018

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Food and Nutrition sciences 2024年第3期15卷 179-198页

作者： Fabio Luis Gazoni Gabriela Miotto Galli Marcel Manente Boiago Lenita Moura stefani Aline Zampar Marco A. Juárez-Estrada Guillermo Tellez-Isaias Aleksandro s. da silva Animal Health and Reproduction Veterinary Medicine Graduate Program Federal University of Santa Maria (UFSM) Santa Maria Brazil Animal science Graduate Program Federal University of Rio Grande do Sul (UFRGS) Porto Alegre Brazil Animal science Graduate Program Santa Catarina State University (UDESC) Chapecó Brazil Departamento de Medicina y Zootecnia de Aves Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autonoma de Mexico Ciudad Universitaria Ciudad de Mexico Mexico Department of Poultry science Division of Agriculture University of Arkansas Fayetteville AR USA

Coccidiosis is a disease caused by intracellular protozoan parasites, specifically belonging to the genus Eimeria. These parasites target the gastrointestinal tract in different types of hosts, causing sores in the intestinal lining. The presence of these lesions reduces the animal’s ability to digest and absorb nutrients, significantly impacting their overall performance. The current study aimed to explore the potential correlation between seasonal variations and the incidence of Eimeria spp-induced lesions in broiler chickens’ gastrointestinal tracts in Brazil from 2012 to 2018. A total of 8,607 broiler chickens, aged 14 to 42 days, were sampled from 103 poultry integrated companies in Brazil to conduct intestinal health examinations. The sampling process involved selecting 3 to 6 chickens from each poultry house for examination. The assessment included various abnormalities such as shedding of intestinal cells and excessive fluid and mucus presence, thickening and tension of the intestines, food movement, roughened mucosal surface resembling a Turkish towel in the small intestine, tissue death, duodenal inflammation, intestinal inflammation, gizzard erosion, presence of worms and bedding material, and ingestion of mealworms. During the seasons, winter exhibited the highest average occurrence of Eimeria maxima microorganisms at 52.83%, with E. acervulina following closely at 26.42% in second place. In spring, E. maxima had an occurrence of 11.31%, while in fall, E. tenella had the lowest occurrence at 6.74%. When analyzing the seasonal occurrence of Eimeria, it was observed that E. maxima micro was more common during winter compared to summer (P = 0.0491). However, no discernible variation was observed in the occurrence of the remaining species across different seasons. Research findings suggest that subclinical coccidiosis is most prevalent during the winter season in Brazil. Likewise, clinical disease caused by E. acervulina is also prevalent during this time. I

关键词： Eimeria Epidemiology Intestinal Pathology Broiler Chickens Brazil

QUAsILINEAR EQUATIONs UsING A LINKING sTRUCTURE WITH CRITICAL NONLINEARITIEs

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Acta Mathematica scientia 2022年第3期42卷 975-1002页

作者： Edcarlos D.silva Jefferson s.silva Federal University of Goi′as Zip code 74001-970Goi′as-GOBrazil

It is to establish existence of a weak solution for quasilinear elliptic problems assuming that the nonlinear term is *** potential V is bounded from below and above by positive *** we are considering a critical term which interacts with higher eigenvalues for the linear problem,we need to apply a linking *** that the lack of compactness,which comes from critical problems and the fact that we are working in the whole space,are some obstacles for us to ensure existence of solutions for quasilinear elliptic *** main feature in this article is to restore some compact results which are essential in variational *** that compactness conditions such as the Palais-smale condition for the associated energy functional is not available in our *** difficulty is overcame by taking into account some fine estimates on the critical level for an auxiliary energy functional.

关键词： Quasilinear schrödinger equations linking theorems superlinear elliptic equations critical nonlinearities Bounded potentials

Bioactive compounds of red grapes from Dao region(Portugal):Evaluation of phenolic and organic profile

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Asian Pacific Journal of Tropical Biomedicine 2016年第4期6卷 315-321页

作者： Luís Rodrigues silva Mafalda Queiroz Health sciences Research Centre Faculty of Health sciences University of Beira Interior Department of Chemical Engineering Faculty of EngineeringUniversity of Porto Polytechnic Institute of Castelo Branco School of Health Dr.Lopes Dias Laboratory of Pharmacognosy Department of ChemistryFaculty of PharmacyUniversity of Porto

Objective:To improve the knowledge on the metabolite profile of five red grapes from D?o region(Portugal),concerning to the phenolic characteristics(coloured and non-coloured phenolics) and organic acid ***:Five red grapes collected from D?o region were *** profiles of phenolic compounds and organic acids were estimated by high-performance liquid chromatography with diode-array detection and high-performance liquid chromatography with UV detector,***:Totally 24 phenolic compounds were identified,and distributed by several classes:8 anthocyanins,1 hydroxybenzoic acid,4 hydroxycinnamic acids,1 stilbene,4flavan-3-ols,6 ***,10 organic acids were detected in all *** contents of each phenolic class and organic acids amounts varied significantly among the different grape cultivars *** principal components analysis differentiates the Touriga Nacional from the other varieties due to their high contents in anthocyanins,non-coloured phenolics and organic *** Nacional is an important red grape cultivar,highly esteemed in D?o region for its ability to produce high quality ***:The results suggest that the red grapes from D?o region present a good composition in bioactive compounds,being important for the production of wines with superior quality.

关键词： Vitis vinifera Grapes Dao region Phenolics Organic acids

Factors determining the occupancy of nest-boxes by Great Tits(Parus major)in eucalypt plantations

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Avian Research 2023年第2期14卷 200-205页

作者： Ricardo s.Ceia Pedro B.Lopes Luís Pda silva Centre for Functional Ecology–science for People&the Planet Department of Life SciencesUniversity of Coimbra3000-456CoimbraPortugal CIBIO Centro de Investigaçao em Biodiversidade e Recursos GeneticosInBIO Laboratorio AssociadoCampus de Vair aoUniversidade do Porto4485-661VairaoPortugal BIOPOLIs Program in Genomics Biodiversity and Land PlanningCIBIOCampus de Vai~ao4485-661VairaoPortugal

Providing nest-boxes as surrogate tree cavities can be of great importance to increase the breeding populations of cavity-nesting birds in managed ***,the exact placement of nest-boxes should be taken into consideration to enhance their occupancy according to species-specific *** this study,we investigated which factors can better predict nest-box occupancy by the Great Tit(Parus major)in eucalypt *** used generalised linear mixed-effects models to analyse the influence of topography,nest-box positioning,vegetation cover and landscape variables on three-year occupancy records from 80 newly provided ***-random patterns of nest-box occupancy were found with respect to all categories except *** suggest that Great Tits prefer to occupy high-placed nest-boxes,close to areas that can provide them with supplementary resources either within or in the vicinity of the stand(i.e.,trees other than eucalypts,riparian vegetation,and large patches of adjacent habitats).Overall,this study provides important recommendations for nest-box placement and spatial distribution in managed forests and enhances the potential of nest-box interventions as a biodiversity offset and management tool.

关键词： Eucalyptus globulus Managed forest Nest-site attributes secondary cavity-nester species-specific preferences Tit

Modeling size-number Distributions of seeds for Use in soil Bank studies

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Journal of Integrative Plant Biology 2008年第5期50卷 531-535页

作者： Hugo Casco Alexandra soveral Dias Luís silva Dias Chemical Ecology Unit Centre of Ecology and EnvironmentUniversidade de vora7002-554 vora Portugal

Knowledge of soil seed banks is essential to understand the dynamics of plant populations and communities and would greatly benefit from the integration of existing knowledge on ecological correlations of seed size and shape. The present study aims to establish a feasible and meaningful method to describe size-number distributions of seeds in multi-species situations. For that purpose, size-number distributions of seeds with known length, width and thickness were determined by sequential sieving. The most appropriate combination of sieves and seeds dimensions was established, and the adequacy of the power function and the Weibull model to describe size-number distributions of spherical, non-spherical, and all seeds was investigated. We found that the geometric mean of seed length, width and thickness was the most adequate size estimator, providing shape-independent measures of seeds volume directly related to sieves mesh side, and that both the power function and the Weibull model provide high quality descriptions of size-number distributions of spherical, non-spherical, and all seeds. We also found that, in spite of its slightly lower accuracy, the power function is, at this stage, a more trustworthy model to characterize size-number distributions of seeds in soil banks because in some Weibull equations the estimates of the scale parameter were not acceptable.

关键词： accuracy power function seeds shape seeds size size-number distributions Weibull model.

The Use of secondary Grape Biomass in Beef Cattle Nutrition on Carcass Characteristics, Quality and shelf Life of Meat

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Food and Nutrition sciences 2024年第6期15卷 447-469页

作者： Vitor L. Molosse Guilherme L. Deolindo Rafael V. P. Lago Bruna Klein Claiton A. Zotti Marcelo Vedovato Marcylene V. da silveira Priscila M. Copetti Maria R. C. schetinger Juscivete F. Favero Eliana L. Fiorentin Aleksandro s. da silva Universidade do Estado de santa Catarina (UDEsC) Chapec Brazil Department of Animal science UDESC Chapec Brazil Department of Animal science Universidade do Oeste de Santa Catarina Xanxere Brazil Dean Lee Research and Extension Center Louisiana State University Alexandria LA USA Department of Biochemistry Universidade Federal de Santa Maria Santa Maria Brazil Department of Medicina Veterinria UNOCHAPEC Chapec Brasil

We determined whether the inclusion of 100 g/kg dry matter of grape pomace silage (GPs) and grape pomace bran (GPB) as substitutes for other traditional fiber sources in the diet of steers (Charolais x Nellore) would improve carcass characteristics, meat quality and composition, and shelf life. Twenty-four animals (248 ± 19.32 kg of initial body weight) were fed a high concentrate diet for 121 days. Carcass characteristics were measured, and the longissimus dorsi muscle was analyzed for fatty acid (FA) profile and composition. The meat was sliced and stored in air-permeable packages for 10 days. On each sampling day (d 1, 3, 7, and 10), oxidative stability, bacterial load, lipid and protein oxidation, and staining were analyzed. The experimental diets influenced the pH of cold carcasses only. The GPs group had a higher pH than the control. The GPs and GPB groups showed improved oxidant status (i.e., lower lipid peroxidation and concentrations of reactive oxygen species were in the meat of both groups than in control). On the first day of storage, the antioxidant enzyme glutathione s-transferase activity was more significant in the meat of the GPs and GPB groups than in the control. The bacterial loads in the meat were attenuated by GPs inclusion;there were lower total coliform counts and a trend toward lower counts for enterobacteria in the control group. The diets altered the FA profile of the meat;i.e., the GPB diet allowed for a more significant amount of the n-6 omegas in the meat, while the GPs diet showed a tendency for a more significant amount of n-6 and 9 omegas. Both diets (GPs and GPB) increased the amounts of long-chain FAs. The GPs diet decreased saturated FA levels. We conclude that the dietary treatments GPs and GPB are a promising alternative to maintain meat quality standards throughout in real-world retail conditions. These treatments gave rise to an improvement in the nutritional value of the meat due to the more significant amounts of FAs that im

关键词： Animal Nutrition Antioxidant Biomass Grape Meat Quality Microbiology Residue

Ultrafast synthesis and sintering of materials in a single running experiment approach by using electric fields

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Journal of Advanced Ceramics 2019年第2期8卷 265-277页

作者： Lílian M.JEsUs Ronaldo s.silva Jean-Claude M’PEKO sao Carlos Institute of Physics University of Sao Paulo(USP)P.O.Box:369CEP:13560-970 Sao CarlosSPBrazil Group of Advanced Ceramic Materials Department of PhysicsFederal University of Sergipe(UFS)CEP:49100-000 Sao CristovaoSEBrazil Department of Physics Federal University of Sao Carlos(UFSCar)13560-905 Sao CarlosSPBrazil

Processing of materials in the form of ceramics normally involves several steps including calcination at a relatively low temperature for synthesis of the end-product powder and sintering at a high temperature for *** work we have been developing introduces a novel approach enabling synthesis plus sintering of materials in a single running experiment by using electric fields,ending with dense ceramics that display grains noticeably finer than in conventional *** new paradigm is fully illustrated with experiments conducted on amorphous CaCu3Ti4O12 precursor powder,shown to experience,on heating,crystallization through intermediate phases,followed by chemical reaction leading to synthesis of the end-product powder,plus densification depending on field *** processing time and furnace temperature are considerably reduced,demonstrating that enhanced synthesis and sintering rates applied under field *** results found in Bi2/3Cu3Ti4O12are also *** different factors that may contribute to this unique scenario,including Joule heating,defect generation,and reduction of free energy for nuclei formation promoted by the applied field,are briefly ***,the findings we bring here are exclusive as they show an exploitable way that allows rapid processing of materials with good control over particle and grain coarsening.

关键词： field-assisted processing synthesis sintering microstructure dielectric response

Layered double hydroxide(LDH)-based materials:A mini-review on strategies to improve the performance for photocatalytic water splitting

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Journal of Energy Chemistry 2022年第1期31卷 406-431,I0011页

作者： Hanane Boumeriame Eliana s.Da silva Alexey s.Cherevan Tarik Chafik Joaquim L.Faria Dominik Eder Laboratory of separation and Reaction Engineering-Laboratory of Catalysis and Materials(LsRE-LCM) Faculdade de EngenhariaUniversidade do PortoRua Dr Roberto Frias s/n4200-465 PortoPortugal Institute of Materials Chemistry Technische Universicat Wien(TU Wien)Getreidemarkt 9Vienna 1060Austria Laboratory of Chemical Engineering and Valorization of Resources(LGCVR-UAE/L01FsT).Faculte des sciences et Techniques Universite Abdelmalek EssaadiTangierMorocco

The high energy demand we currently face in society and the subsequent large consumption of fossil fuels cause its depletion and increase the pollution *** quest for the production of clean energy from renewable and sustainable sources remains *** conversion of solar energy into hydrogen via the water-splitting process,assisted by pho tores pons ive semiconductor catalysts,is one of the most promising *** progress has been made on water splitting in the past few years and a variety of photocatalysts active not only under ultra-violet(UV) light but especially with the visible part of the electromagnetic spectrum have been *** double hydroxides(LDH)-based materials have emerged as a promising class of nanomaterials for solar energy applications owing to their unique layered structure,compositional flexibility,tunable bandgaps,ease of synthesis and low manufacturing *** review covers the most recent research dedicated to LDH materials for photocatalytic water-splitting applications and encompasses a range of synthetic strategies and post-modifications used to enhance their ***,we provide a thorough discussion of the experimental conditions crucial to obtaining improved photoactivity and highlight the impact of some specific parameters,namely,catalysts loading,cocatalysts,sacrificial agents,and irradiation *** review provides the necessary tools to select the election technique for adequately enhancing the photoactivity of LDH and modified LDH-based materials and concludes with a critical summary that outlines further research directions.

关键词： Layered double hydroxides Water splitting Photocatalysis Hydrogen generation Oxygen evolution Nanomaterials