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二抗物种特异性对双色STORM成像的影响(特邀)

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激光与光电子学进展 2024年第6期61卷 145-152页

作者：邓师禹胡芬侯梦迪杨建宇李任植潘雷霆弱光非线性光子学教育部重点实验室南开大学物理科学学院泰达应用物理研究院天津300071 药物化学生物学全国重点实验室南开大学生命科学学院细胞应答交叉科学中心天津300071 南开大学深圳研究院广东深圳518083 极端光学协同创新中心山西大学山西太原030006

随机光学重建显微术(STORM)基于免疫荧光标记技术,具有原理易懂、光路简单、分辨率极高等特点,一直受到科研工作者的青睐,但分辨率的提升对抗体的特异性提出了更高的要求。相较一抗直接标记,“一抗+二抗”的间接标记法在实际应用中普适... 详细信息

随机光学重建显微术(STORM)基于免疫荧光标记技术,具有原理易懂、光路简单、分辨率极高等特点,一直受到科研工作者的青睐,但分辨率的提升对抗体的特异性提出了更高的要求。相较一抗直接标记,“一抗+二抗”的间接标记法在实际应用中普适性更强。二抗相对一抗存在物种特异性的问题,生产时需要对其进行预吸附来提升物种特异性。为了探究二抗物种特异性对双色STORM成像的影响,基于经典的红细胞骨架模型中血影蛋白N端和C端的互斥位置关系,对二者使用高、低吸附二抗标记后分别进行双色STORM成像,对照模拟中有无信号串扰条件下的互相关分析结果,结果表明低吸附二抗会造成二者共定位的假象。进一步,分别通过高、低吸附二抗对MDA-MB-231乳腺癌细胞CD47和PD-L1两种膜蛋白进行双色STORM成像,结果揭示两种蛋白无共定位关系。本研究为二抗物种特异性的评估提供了一种基于红细胞骨架结构模型的超分辨成像新策略,助力双色STORM成像精准阐明蛋白分子互作关系。

关键词：超分辨成像随机光学重建显微术免疫荧光红细胞膜骨架互相关分析

Regulation of actin cytoskeleton via photolithographic micropatterning

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Journal of Innovative Optical Health Sciences 2023年第2期16卷 50-57页

作者： Fulin Xing Haimei Zhang Mengyu Li Hao Dong Xuehe Ma Shiyu Deng Fen Hu imshik lee Leiting Pan Jingjun Xu The Key Laboratory of Weak-Light Nonlinear Photonics of Education Ministry School of Physics and TEDA Institute of Applied Physics Nankai University Tianjin 300071 P.R.China State Key Laboratory of Medicinal Chemical Biology Frontiers Science Center for Cell Responses College of Life Sciences Nankai University Tianjin 300071P.R.China Collaborative Innovation Center of Extreme Optics Shanxi University Taiyuan Shanxi 030006P.R.China Shenzhen Research Institute of Nankai University Shenzhen Guangdong 518083P.R.China

Actin cytoskeleton plays crucial roles in various cellular *** matrix(ECM)can modulate cell morphology by remodeling the internal *** define how geometry of ECM regulates the organization of actin cytoskeleton,we plated individual NIH 3T3 cells on micropatterned substrates with distinct shapes and *** was found that the stress fibers could form along the nonadhesive edges of T-shaped pattern,but were absent from the opening edge of V-shaped pattern,indicating that the organization of actin cytoskeleton was dependent on the mechanical ***,a secondary actin ring was observed on 50μm circular pattern while did not appear on 30μm and 40μm pattern,showing a size-dependent organization of actin ***,osteoblasts,MDCK and A549 cells exhibited distinct organization of actin cytoskeleton on T-shaped pattern,suggesting a cell-type specificity in arrangement of actin ***,our findings brought novel insight into the organization of actin cytoskeleton on micropatterned environments.

关键词： Actin cytoskeleton photolithography micropatterning extracellular matrix

Conformational Studies of Arabinoglucuronomannology (AGM)

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生物物理学报 1996年第4期12卷 547-553页

作者： imshik lee Bai Chunli Wang Chen (Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100080) 中科院化学所北京

ＣｏｎｆｏｒｍａｔｉｏｎａｌＳｔｕｄｉｅｓｏｆＡｒａｂｉｎｏｇｌｕｃｕｒｏｎｏｍａｎｎｏｌｏｇｙ（ＡＧＭ）ＩｍｓｈｉｋＬｅｅ；ＢａｉＣｈｕｎｌｉ；ＷａｎｇＣｈｅｎ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｅｍｉｓｔｒｙ，Ｃｈｉｎｅ... 详细信息

ＣｏｎｆｏｒｍａｔｉｏｎａｌＳｔｕｄｉｅｓｏｆＡｒａｂｉｎｏｇｌｕｃｕｒｏｎｏｍａｎｎｏｌｏｇｙ（ＡＧＭ）ＩｍｓｈｉｋＬｅｅ；ＢａｉＣｈｕｎｌｉ；ＷａｎｇＣｈｅｎ（ＩｎｓｔｉｔｕｔｅｏｆＣｈｅｍｉｓｔｒｙ，ＣｈｉｎｅｓｅＡｃａｄｅｍｙｏｆＳｃｉｅｎｃ...

关键词： Arabinoglucuronomannology(AGM) X - ray diffraction Conformation Relative humidity (RH)

Structure Studies on Host-Guest Recognition Sensory Systems

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Chinese Chemical Letters 1997年第8期8卷 707-710页

作者： Lin Jing YANG Xi Zeng FENG imshik lee Chun Li BAI(Institute of Chemistry, the Chinese Academy of Science,Beijing, 100080) Institute of Chemistry Chinese Academy of Science Beijing 100080 China

Rhodamine B-ethylenediamine-beta-cyclodextrins (RhB-beta-CDen) and rhodamine B-beta-cyclodextrins (RhB-beta-CD) form inclusion complexes with many guest molecules, which can be used as nucleic acid probe. In this paper we determined the most stable conformations of RhB-beta-CDen and RhB-beta-CD by molecular mechanics and dynamics simulation. The interaction between RhB-beta-CDen and two guest molecules, 1-borneol and cyclohexanol, have been investigated both theoretically and experimentally. The results show that the interaction between borneol and RhB-beta-CDen is stronger than that between cyclohexanol and RhB-beta-CDen.

关键词： RhB Structure Studies on Host-Guest Recognition Sensory Systems

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Scanning Tunneling Microscopy Studies of Agal Polysaccharide:Gelation of Carrageenan

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Scanning Tunneling Microscopy Studies of Agal Polysaccharide...

Scanning Tunneling Microscopy Studies of Agal Polysaccharide: Gelation of Carrageenan

第四届全国STM学术会议

作者： lee imshik Institute of Chemistry Chinese Academy of ScienoesBeijing 100080

＜正＞Carrageenan was visualized by computer modeling and by *** aggregated and monola- yered carrageenan were observed on the surface of *** morphology contains a certain orientation in chain arrangement.A localized orientation of chain arrangement may relate to the properties of HPOG surface,the polyanionic distribution along the chain axis and the chain kinking. The width of a highly packed-image was～2nm,while that of aggregated one 4—5nm.

关键词： aggregation helix polyanions chain arrangement

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电子显微学报 1997年第6期 22-25页

作者： lee imshik,Bai Chunli,Wang Chen(Institute of Chemistry,Chinese Academy of Sciences,Beijing 100080)

ＳｃａｎｎｉｎｇＴｕｎｎｅｌｉｎｇＭｉｃｒｏｓｃｏｐｙＳｔｕｄｉｅｓｏｆＡｇａｌＰｏｌｙｓａｃｃｈａｒｉｄｅ：ＧｅｌａｔｉｏｎｏｆＣａｒｒａｇｅｅｎａｎTXＳｃａｎｎｉｎｇＴｕｎｎｅｌｉｎｇＭｉｃｒｏｓｃｏｐｙＳｔｕｄ... 详细信息

ＳｃａｎｎｉｎｇＴｕｎｎｅｌｉｎｇＭｉｃｒｏｓｃｏｐｙＳｔｕｄｉｅｓｏｆＡｇａｌＰｏｌｙｓａｃｃｈａｒｉｄｅ：ＧｅｌａｔｉｏｎｏｆＣａｒｒａｇｅｅｎａｎTXＳｃａｎｎｉｎｇＴｕｎｎｅｌｉｎｇＭｉｃｒｏｓｃｏｐｙＳｔｕｄｉｅｓｏｆＡｇａｌＰｏｌｙｓａｃｃ...

关键词： aggregation helix polyanions chain arrangement

Regulation of intracellular Ca2+/CaMKII signaling by TRPV4 membrane translocation during osteoblastic differentiation

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Biophysics Reports 2019年第Z1期5卷 254-263页

作者： Fen Hu Yali Zhao Zhenhai Hui Fulin Xing Jianyu Yang imshik lee Xinzheng Zhang Leiting Pan Jingjun Xu The Key Laboratory of Weak-Light Nonlinear Photonics Ministry of Education School of Physics and TEDA Institute of Applied Physics Nankai University Collaborative Innovation Center of Extreme Optics Shanxi University

Bone constantly remodels between resorption by osteoclasts and formation by osteoblasts; therefore the functions of osteoblasts are pivotal for maintaining homeostasis of bone mass. Transient receptor potential vanilloid 4(TRPV4), a type of mechanosensitive channel, has been reported to be a key regulator in bone remodeling. However, the relationship between TRPV4 and osteoblast function remains largely elusive. Only little is known about the spatial distribution change of TRPV4 during osteoblastic differentiation and related signal events. Based on three-dimensional super-resolution microscopy, our results clearly showed a different distribution of TRPV4 in undifferentiated and differentiated osteoblasts, which reflected the plasma membrane translocation of TRPV4 along with prolonged differentiation. GSK1016790 A(GSK101), the most potent agonist of TRPV4, triggered rapid calcium entry and calmodulin-dependent protein kinase II(Ca MKII) phosphorylation via TRPV4 activation in a differentiation-dependent manner, indicating that the abundance of TRPV4 at the cell surface resulting from differentiation may be related to the modulation of Ca2+response and Ca MKII *** data provide compelling evidences for the plasma membrane translocation of TRPV4 during osteoblastic differentiation as well as demonstrate the regulation of downstream Ca2+/Ca MKII signaling.

关键词： Osteoblasts Differentiation TRPV4 channel Plasma membrane translocation Super-resolution microscopy Ca~(2+) signaling

αⅡbβ3同源模拟和环形RGD药物小分子设计

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生物工程学报 2008年第2期24卷 297-301页

作者：罗明艳陈梅宗李任植南开大学物理科学学院

血小板表面的整合蛋白α_(Ⅱb)β_3对血栓的形成具有很重要的调控作用,α_(Ⅱb)β_3与纤维蛋白原上的RGD特征序列结合,促进血小板凝集进而形成血栓。这样可以在理论上设计一个环形RGD小分子(RGD-c)与α_(Ⅱb)β_3蛋白结合,阻断其与纤维... 详细信息

血小板表面的整合蛋白α_(Ⅱb)β_3对血栓的形成具有很重要的调控作用,α_(Ⅱb)β_3与纤维蛋白原上的RGD特征序列结合,促进血小板凝集进而形成血栓。这样可以在理论上设计一个环形RGD小分子(RGD-c)与α_(Ⅱb)β_3蛋白结合,阻断其与纤维蛋白原的结合位点,进而阻断血栓形成。以糖蛋白α_vβ_3(pdb代码1jv2)作为模板,利用modeller8v2软件进行同源模拟得到α_(Ⅱb)β_3三维模型。而小分子则以RGD序列为主,两边各加一个氨基酸X、Y,X和Y之间用一个二硫键相连,通过改变X和Y,重复优化过程则可得到不同的小分子模型,将其与α_(Ⅱb)β_3进行分子对接,可以找出比较理想的XY组合。

关键词： αⅡbβ3 同源模拟对接 RGD-C MM

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臭氧对胎牛血清氧化损伤的表面增强拉曼光谱

光谱学与光谱分析 2007年第6期27卷 1140-1142页

作者：邹祖全刘燕楠吴瑛李奇楠陈梅宗孙海英李任植南开大学物理科学学院生物物理系天津300071 南开大学物理科学学院光子学中心国家纳米技术与工程研究院天津300457

对实验室细胞培养用的国产胎牛血清在臭氧作用前后进行了表面增强拉曼光谱测试,范围200～1800cm-1。发现臭氧作用1h后拉曼谱线强度大大减弱,作用3h后谱线强度进一步减弱,但减弱的程度明显减慢,说明臭氧氧化作用具有快速的特点。臭氧作... 详细信息

对实验室细胞培养用的国产胎牛血清在臭氧作用前后进行了表面增强拉曼光谱测试,范围200～1800cm-1。发现臭氧作用1h后拉曼谱线强度大大减弱,作用3h后谱线强度进一步减弱,但减弱的程度明显减慢,说明臭氧氧化作用具有快速的特点。臭氧作用下蛋白质主链的有序构象(α-螺旋,β折叠)明显减少,对β折叠的损伤更为严重,β回折几乎消失。芳香族氨基酸和半胱氨酸侧链在臭氧作用下氧化损伤非常明显。这些特征谱线的变化,反映了臭氧的氧化作用使蛋白质变性,构象变化以及降解。

关键词：表面增强拉曼光谱胎牛血清臭氧

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