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Journal of Integrative Agriculture 2019年第2期18卷 381-394页

作者： Gerald Zvobgo Jonas Lwalaba Wa Lwalaba Tichaona Sagonda James Mutemachani Mapodzeke Noor Muhammad imran haider shamsi ZHANG Guo-ping Department of Agronomy College of Agriculture and BiotechnologyZhejiang University/Zhejiang Key Laboratory of Crop Germplasm ResourceHangzhou 310058P.R.China

Arsenic(As) contamination in soils has posed a severe threat to safe crop production. The previous studies showed the antagonism between phosphorus(P) and As in plant growth and As uptake, while the mechanisms of alleviating As toxicity by P is not completely clear. Due to the limiting P condition, it is imperative to understand how low P addition can be used to suppress arsenate As(V) uptake and the subsequent mechanisms involved. Thus in this study we investigated the effect of P addition on As uptake, anti-oxidative enzyme activity, and anti-oxidant content, and the relative expression of transport, defense, and detoxification genes using two barley genotypes differing in As toxicity tolerance. P addition significantly reduced As concentration in plant tissues, and caused the great changes in activities of catalase and superoxide dismutase, glutathione content, and the relative expression of examined genes when the plants of the two barley genotypes were exposed to 100 μmol L^(–1) As, with ZDB160(As-tolerant) being much more affected than ZDB475(As-sensitive). The current results show that P addition can alleviate As toxicity by regulating the expression of As transport, defense, and detoxification genes to a greater extent in As tolerance of barley, suggesting the possibility of controlling As uptake and toxicity by applying low amount of P fertilizers in the As-contaminated soils.

关键词： arsenate phosphate transporter tolerance toxicity

Detection of Tocopherol in Oilseed Rape (Brassica napus L.) Using Gas Chromatography with Flame Ionization Detector

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Journal of Integrative Agriculture 2013年第5期12卷 803-814页

作者： Nazim Hussain Zahra Jabeen LI Yuan-long CHEN Ming-xun LI Zhi-lan GUO Wan-li imran haider shamsi CHEN Xiao-yang JIANG Li-xi Key Laboratory of Crop Germplasm Resources of Zhejiang Province College of Agriculture and BiotechnologyZhejiang University

The variation among Chinese genotypes of Brassica napus L. for seed tocopherols content and their analysis using gas chromatography has not been comprehensively reported till to date. In the present study, the tocopherol contents of four Chinese genotypes of Brassica napus L., namely, Gaoyou 605, Zhejiang 619, Zheshuang 758, and Zheshuang 72, were evaluated using three modified sample preparation protocols （P1, P2, and P3） for tocopherol extraction. These methods were distinguished as follows. Protocol one （P1） included the evaporation of solvent after extraction without silylation. Protocol two （P2） followed the direct supernatant collection after overnight extraction without drying and silylation. Protocol three （P3） included trimethylsilylation with N,O-bis（trimethylsilyl） trifluoroacetamide. Genotypic comparison of tocopherol and its isoforms revealed that Gaoyou 605 was dominant over the other genotypes with （140.5＋ 10.5）, （316.2＋ 9.2）, and （559.1＋ 24.3） ~tg g-~ of seed meal ct-, 7-, and total （T-） tocopherol, respectively, and a 0.44＋0.04 ^- to 7-tocopherol ratio. The comparison of the sample preparation protocols, on the other hand, suggests that P3 is the most suitable method for the tocopherol extraction from Brassica oilseeds and for the analysis of tocopherols using gas chromatography flame ionization detector （GC-FID）. Trimethylsilylation is the key step differentiating P3 from P1 and P2. Variations detected in tocopherol contents among the Chinese rapeseed （B. napus） genotypes signify the need to quantify a wide range of rapeseed germplasm for seed tocopherol dynamics in short and crop improvement in long.

关键词： Brassica napus L. genotypic variation tocopherol analysis gas chromatography flame ionization detector

巴基斯坦高校创业教育探究--一项教师视角的案例研究

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现代大学教育 2015年第4期31卷 40-47页

作者： Iffat Batool 徐小洲 imran haider shamsi 孙杨李娜浙江大学教育学院南京大学教育研究院哈尔滨师范大学教育科学学院

作为一个发展中国家,巴基斯坦面临着贫穷、失业等诸多问题,关注高校创业教育并给予实践,鼓励和引导创业,将极大地促进就业率,为发展巴基斯坦国家经济做出巨大贡献。此研究以巴基斯坦两所高校的教师为研究对象,希望通过对巴基斯坦的旁遮... 详细信息

作为一个发展中国家,巴基斯坦面临着贫穷、失业等诸多问题,关注高校创业教育并给予实践,鼓励和引导创业,将极大地促进就业率,为发展巴基斯坦国家经济做出巨大贡献。此研究以巴基斯坦两所高校的教师为研究对象,希望通过对巴基斯坦的旁遮普大学和白沙瓦大学的创业教育调研来进一步认识巴基斯坦高校创业教育的现状。目前巴基斯坦高校创业教育存在着诸如体制不均衡、课程改革滞后等问题,提升巴基斯坦创业教育水平需要政府和高校的共同努力。

关键词：巴基斯坦高等院校创业教育教师

茉莉酸对油菜(Brassica napus L.)受镉毒害的缓解作用（英文）

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2018年第2期19卷 130-146页

作者： Essa ALI Nazim HUSSAIN imran haider shamsi Zahra JABEEN Muzammil Hussain SIDDIQUI Li-xi JIANG Zhejiang Key Laboratory of Crop Gene Resources College of Agriculture and Biotechnology Zhejiang University Faculty of Agriculture the University of Poonch Department of Biosciences COMSATS Institute of Information Technology

目的:本研究目的在于了解:(1)喷施外源茉莉酸对受到镉胁迫油菜的作用;(2)是否茉莉酸能够通过增强气体交换,从而保护受到氧化胁迫的地上部分组织的叶绿体,进而通过减少镉的吸收来维持离子平衡;(3)是否通过喷施茉莉酸来对具有减缓镉毒害... 详细信息

目的:本研究目的在于了解:(1)喷施外源茉莉酸对受到镉胁迫油菜的作用;(2)是否茉莉酸能够通过增强气体交换,从而保护受到氧化胁迫的地上部分组织的叶绿体,进而通过减少镉的吸收来维持离子平衡;(3)是否通过喷施茉莉酸来对具有减缓镉毒害效应的抗氧化酶的活性进行调节。创新点:茉莉酸能够调节响应胁迫的抗氧化酶的活性,从而通过保护叶绿体免受活性氧(ROS)伤害而提高光合产物的能力,最大限度地缓解油菜植株受到的镉毒害。方法:(1)叶片气体交换;(2)叶片光合色素分析;(3)丙二醛与抗氧化酶活性分析;(4)营养成分分析;(5)透射电镜亚细胞水平观察。结论:茉莉酸对于植物受镉毒害的缓解作用的机理在于减少叶片中镉的积累,从而减轻氧化胁迫过程中产生的ROS对于膜系统的损害程度。

关键词：油菜镉茉莉酸抗氧化酶丙二醛超微结构

Subcellular distribution of Arsenic(As)and its chemical forms in tobacco genotypes differing in Arsenic tolerance

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Subcellular distribution of Arsenic(As)and its chemical form...

氮肥种类与施量对油菜种子生育酚的影响(英文)

2014年中国作物学会学术年会

作者： Gerald Zvobgo imran haider shamsi Guoping Zhang Department of Agronomy Key Laboratory of Crop Germplasm Resource of Zhejiang ProvinceZhejiang University

Arsenic(As)accumulation and toxicity in plants differs greatly among plant species and also among organs or tissues in the same *** in order to understand As toxicity in plant tissues,it is necessary to study its forms and subcellular *** distribution and As forms were investigated in a hydroponic culture using 2 different As tolerant tobacco(Nicotiana tabacum)genotypes ZY90 and *** As treatments of 1,10 and 100 μM were applied and As concentration was measured in 5 different fractions i.e cell wall,plastid,nucleus,mitochondria and soluble *** results of our study showed that in roots As was mainly localized in cell walls whereas in stems and shoots As was localized in both cell wall and *** is clear and strong evidence that both the cell wall and vacuoles are major barriers in protecting plants against As toxicity.A clear difference was observed between genotypes in As concentration in subcellular and its chemical forms and it was found that the As-sensitive genotype ZY90 in comparison to FSMY contained higher As in plastid,nucleus and mitochondria and lower As in cell wall and soluble *** present study showed that As tolerant plants compartmentalize some of the As into the cell wall and some is sequestrated into the vacuole where it is detoxified thus ensuring the protection against As ***,the sensitive As genotype localized the accumulated As into active *** conclusion we observed that As sensitivity is not brought about only by the amount of As accumulated by a tobacco genotype but how the As is distributed in subcellular organs and tissues does matter a lot.

关键词： Subcellular distribution Arsenic Genotype Compartmentalization Tobacco

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2014年第2期15卷 181-193页

作者： Nazim HUSSAIN Hui LI Yu-xiao JIANG Zahra JABEEN imran haider shamsi Essa ALI Li-xi JIANG Key Laboratory of Crop Gene Resources of Zhejiang Province College of Agriculture and BiotechnologyZhejiang University

研究目的:生育酚是菜籽重要的品质指标,氮肥是影响作物生物学与籽粒产量最常用的肥料。这项研究旨在搞清楚氮肥种类与施量对油菜种子生育酚含量与组分的确切影响,以及这种影响在基因型之间的差异。创新要点:这篇论文研究了不同的氮肥种... 详细信息

研究目的:生育酚是菜籽重要的品质指标,氮肥是影响作物生物学与籽粒产量最常用的肥料。这项研究旨在搞清楚氮肥种类与施量对油菜种子生育酚含量与组分的确切影响,以及这种影响在基因型之间的差异。创新要点:这篇论文研究了不同的氮肥种类(硝态氮与铵态氮)与低、中、高施用量对种子生育酚总量与组分的影响,并分析了其中的原因,为通过合理的氮肥施用方案配置,以达到最理想的菜籽生育酚含量或组分提供依据。研究方法:采用盆钵实验控制氮肥施量与流失的精准方法,五种基因型、二种氮肥种类、三档施量水平,三重复控制误差;尝试用气相色谱法检测菜籽生育酚含量的新方法。重要结论:尿素比硝酸氨更有利于菜籽总生育酚、阿尔法生育酚及伽马生育酚的有效形成;提高氮肥施量对于菜籽形成高含量的总生育酚与伽马生育酚非常有效,但对提高菜籽阿尔法生育酚含量的效果却不太明显。

关键词：油菜氮肥施量尿素硝酸氨生育酚

Recent Developments in Authentication Schemes Used in Machine-Type Communication Devices in Machine-to-Machine Communication: Issues and Challenges

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Computers, Materials & Continua 2024年第4期79卷 93-115页

作者： Shafi Ullah Sibghat Ullah Bazai Mohammad imran Qazi Mudassar Ilyas Abid Mehmood Muhammad Asim Saleem Muhmmad Aasim Rafique Arsalan haider Ilyas Khan Sajid Iqbal Yonis Gulzar Kauser Hameed Department of Computer Engineering Balochistan University of Information TechnologyEngineering and Management SciencesQuetta87300Pakistan Department of Information Technology Balochistan University of Information TechnologyEngineeringand Management SciencesQuetta87300Pakistan Department of Information Systems College of Computer Sciences and Information TechnologyKing Faisal UniversityHofuf in Al-Ahsa31982Saudi Arabia Department of Management Information Systems College of Business AdministrationKing Faisal UniversityHofuf in Al-Ahsa31982Saudi Arabia Department of Software Engineering College of ComputingRiphah International UniversityFaisalabad44000Pakistan Department of Electrical Engineering Balochistan University of Information Technology Engineeringand Management SciencesQuetta87300Pakistan Department of Mathematics College of Science Al-ZulfiMajmaah UniversityAl-Majmaah11952Saudi Arabia

Machine-to-machine (M2M) communication plays a fundamental role in autonomous IoT (Internet of Things)-based infrastructure, a vital part of the fourth industrial revolution. Machine-type communication devices(MTCDs) regularly share extensive data without human intervention while making all types of decisions. Thesedecisions may involve controlling sensitive ventilation systems maintaining uniform temperature, live heartbeatmonitoring, and several different alert systems. Many of these devices simultaneously share data to form anautomated system. The data shared between machine-type communication devices (MTCDs) is prone to risk dueto limited computational power, internal memory, and energy capacity. Therefore, securing the data and devicesbecomes challenging due to factors such as dynamic operational environments, remoteness, harsh conditions,and areas where human physical access is difficult. One of the crucial parts of securing MTCDs and data isauthentication, where each devicemust be verified before data transmission. SeveralM2Mauthentication schemeshave been proposed in the literature, however, the literature lacks a comprehensive overview of current M2Mauthentication techniques and the challenges associated with them. To utilize a suitable authentication schemefor specific scenarios, it is important to understand the challenges associated with it. Therefore, this article fillsthis gap by reviewing the state-of-the-art research on authentication schemes in MTCDs specifically concerningapplication categories, security provisions, and performance efficiency.

关键词： Authentication cyber security internet of things machine-type communication devices machine-to-machine communication

Whole-Genome Resequencing of a Worldwide Collection of Rapeseed Accessions Reveals the Genetic Basis of Ecotype Divergence

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Molecular Plant 2019年第1期12卷 30-43页

作者： Dezhi Wu Zhe Liang Tao Yan Ying Xu Lijie Xuan Juan Tang Gang Zhou Ulrike Lohwasser Shuijin Hua Haoyi Wang Xiaoyang Chen Qian Wang Le Zhu Antony Maodzeka Nazim Hussain Zhilan Li Xuming Li imran haider shamsi Ghulam Jilani Linde Wu Hongkun Zheng Guoping Zhang Boulos Chalhoub Lisha Shen Hao Yu Lixi Jiang institute of Crop Science Zhejiang UniversityHangzhou 310058China Temasek Life Sciences Laboratory and Department of Biological Science National University of SingaporeSingapore 117543Singapore Biomarker Technologies Corporation Beijing 101300China Department of Genebank Leibniz institute of Plant Genetics and Crop Plant Research06466 Stadt SeelandGermany Institute of Crop and Nuclear Agricultural Sciences Zhejiang Academy of Agricultural SciencesHangzhou 310021China Office of Research Innovation & CommercializationPMAS-Arid Agricultural University Rawalpindi46300 RawalpindiPakistan Institute of Crop Science Jinhua Academy of Agncultural SciencesJinhua 321017China

Rapeseed (Brassica napus),an important oilseed crop,has adapted to diverse climate zones and latitudes by forming three main ecotype groups,namely winter,semiwinter,and spring types. However,genetic variations underlying the divergence of these ecotypes are largely unknown. Here,we report the global pattern of genetic polymorphisms in rapeseed determined by resequencing a worldwide collection of 991 germplasm accessions.A total of 5.56 and 5.53 million singlenucleotide polymorphisms (SNPs)as Well as 1.86 and 1.92 million InDels were identified by mapping reads to the reference genomes of "Darmor-bzh"and "Tapidor,"*** generated a map of allelic drift paths that shows splits and mixtures of the main populations,and revealed an asymmetric evolution of the two subgenomes of *** by calculating the genetic diversity and linkage disequilibrium ***-sweep analysis revealed genetic changes in genes orthologous to those regulating various aspects of plant development and response to stresses.A genome-wide association study identified SNPs in the promoter regions of FLOWERING LOCUS T and FLOWERING LOCUS C orthologs that corresponded to the different rapeseed ecotype groups. Our study provides important insights into the genomic footprints of rapeseed evolution and flowering-time divergence among three ecotype groups,and will facilitate screening of molecular markers for accelerating rapeseed breeding.

关键词： Brassica napus genome resequencing selective sweep ecotype divergence GWAS floweringtime trait

Synergistic interaction of NaCl and Cd on growth and photosynthetic parameters in soybean genotypes differing in salinity tolerance

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第4期8卷 266-271页

作者： shamsi imran haider Department of Agronomy Zhejiang UniversityHangzhou 310029China

The effects of salinity(50 mmol/L NaCl) and Cd(1 μmol/L CdCl2) as sole and combined on growth and photosyn-thetic parameters were studied using two soybean genotypes,Huachun 18 and *** concentrations of Cd2+,Zn2+,Ca2+,Mg2+,K+ and Na+ were also determined in seeds and *** 18 suffered a more serious decrease than NGB in net photosynthetic rate(Pn) in the treatments of salinity stress alone and combined stress(NaCl+Cd),showing that it is relatively sensitive to *** decrease in Pn caused by salt stress in Huachun 18 was mainly due to the reduced total chlorophyll content and photosynthetic efficiency(the ratio of variable fluorescence to maximal fluorescence,Fv/Fm),whereas the decease in NGB was mainly related to reduced stomatal conductance(Gs).The combined stress of both Na and Cd did not induce further decrease in photosynthesis and fluorescence in the two genotypes relative to salt or Cd stress *** change in the pod concentrations of Zn2+,Ca2+,Mg2+,K+ and Na+ was detected under salt stress for Huachun 18 than for *** results suggested that the interactive effect of NaCl-Cd on growth and nutrient uptake differs between the two soybean genotypes.

关键词：氯化钠镉大豆基因型耐盐性生长光合参数协同效应

Interactions of cadmium and aluminum toxicity in their effect on growth and physiological parameters in soybean

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2007年第3期8卷 181-188页

作者： shamsi imran haider JILANI Ghulam Department of Agronomy Zhejiang University School of Environment and Resource Sciences Zhejiang University

The effect of Al and Cd on the growth, photosynthesis, and accumulation of Al, Cd and plant nutrients in two soybean genotypes were determined using hydroponic culture. There were six treatments: pH 6.5; pH 4.0; pH 6.5+1.0 μmol/L Cd; pH 4.0+1.0 μmol/L Cd; pH 4.0+150 μmol/L Al; pH 4.0+1.0 μmol/L Cd+150 μmol/L Al. The low pH (4.0) and Al treatments caused marked reduction in root length, shoot height, dry weight, chlorophyll content (SPAD value) and photosynthetic rate. Al-sensitive cv. Zhechun 2 accumulated comparatively more Al and Cd in plants than Al-tolerant cv. Liao 1. Compared with pH 6.5, pH 4.0 resulted in significant increase in Cd and Al concentration in plants. Combined application of Cd and Al enhanced their accu-mulation in roots, but caused a reduction in shoots. The concentrations of all 10 nutrients (P, K, Ca, Mg, Fe, Mn, Cu, Zn and B), except Mo were also increased when plants were exposed to pH lower than pH 6.5. Al addition caused a reduction in the con-centration of most nutrients in plant roots and shoots; but K, Mn and Zn in roots were increased. Treatments with Cd alone or together with Al reduced the concentrations of all the plant nutrients in plants. Al-sensitive genotype Zhechun 2 has lower nutrient concentration than Al-tolerant genotype Liao 1. The current findings imply that Al and Cd are synergistic in their effect on plant growth, physiological traits and nutrient uptake.

关键词： Soybean Cadmium Aluminum Photosynthesis Antagonism