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纳豆芽孢杆菌B4芽孢对体外小鼠巨噬细胞活力与功能的影响

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纳豆芽孢杆菌B4芽孢对体外小鼠巨噬细胞活力与功能的影响

中国畜牧兽医学会动物营养学分会第十一次全国动物营养学术研讨会

作者：徐歆黄琴毛予龙黄怡 imran R.Rajput 余东游李卫芬浙江大学动物科学学院饲料科学研究所教育部动物分子营养学重点实验室广西大学动物科学技术学院

本试验旨在探讨纳豆芽孢杆菌B4对体外小鼠巨噬细胞活力与功能的影响。RAW264.7小鼠单核巨噬细胞(1×10个/ml)按每孔1ml接种6孔培养板,37℃、5%CO培养48h后,处理组每孔加入1ml芽孢杆菌B4芽孢悬液(1×10个/ml),对照组加入等体积培养基,37... 详细信息

本试验旨在探讨纳豆芽孢杆菌B4对体外小鼠巨噬细胞活力与功能的影响。RAW264.7小鼠单核巨噬细胞(1×10个/ml)按每孔1ml接种6孔培养板,37℃、5%CO培养48h后,处理组每孔加入1ml芽孢杆菌B4芽孢悬液(1×10个/ml),对照组加入等体积培养基,37℃、5%CO继续培养12h。试验结束后,收集样品并测定指标:(1)台盼蓝拒染法测定巨噬细胞活力;(2)收集培养上清,测定其中LDH活力与NO、细胞因子(TNF-α,IFN-γ,IL-1β,IL-6,IL-8,IL-12,IL-10)水平;(3)加入0.1%Triton X-100裂解细胞并收集细胞裂解液,测定其中ACP、LDH和iNOS活力。结果显示:(1)处理组与对照组的巨噬细胞活力与胞外LDH活力均不存在显著差异(P>0.05),且两组的细胞活力均高于97%,表明纳豆芽孢杆菌B4对小鼠巨噬细胞没有明显的细胞毒性。(2)与对照组相比,处理组巨噬细胞ACP与LDH活力分别极显著升高330.68%(P<0.01)与31.91%(P<0.01),表明纳豆芽孢杆菌B4能够显著激活小鼠巨噬细胞。(3)处理组巨噬细胞iNOS活力与NO水平分别比对照组提高了31.69%(P<0.01)与32.97%(P<0.01),表明纳豆芽孢杆菌B4能够通过显著增强iNOS活力,诱导小鼠巨噬细胞产生NO。(4)与对照组相比,处理组TNF-α、IFN-γ、IL-1β、IL-6、IL-8、IL-12与IL-10水平均极显著增加761.64%(P<0.01)、108.95%(P<0.01)、无限(P<0.01)、79.52%(P<0.01)、286.69%(P<0.01)、无限(P<0.01)和572.08%(P<0.01),表明纳豆芽孢杆菌B4能够显著诱导小鼠巨噬细胞产生促炎和抑炎因子,从而提高其炎症反应。结果提示,纳豆芽孢杆菌B4并未对巨噬细胞造成损伤,因而具有很好的安全性,同时,其一方面能够通过激活巨噬细胞提高其吞噬活性,另一方面可以通过诱导NO与细胞因子产生提高小鼠巨噬细胞的免疫功能,因此,纳豆芽孢杆菌B4可作为一种具有免疫调节活性的益生菌。

关键词：纳豆芽孢杆菌巨噬细胞功能免疫调节小鼠

不同扩张器置入术对颈胸部瘢痕的治疗效果比较

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河南医学研究 2018年第13期27卷 2310-2313页

作者：杨荣强王常印张豫峰孟庆楠高娅王晓博 imran Khan 刘建红崔正军郑州大学第一附属医院烧伤与修复重建外科河南郑州450002 郑州大学第一附属医院微创外科河南郑州450002 中国人民解放军火箭军总医院礼士路门诊部北京100820

目的对比观察不同扩张器置入术对颈胸部瘢痕的治疗效果。方法以郑州大学第一附属医院烧伤与修复重建外科2012年3月至2015年8月入院选择扩张器置入手术治疗的23例颈胸部瘢痕患者作为研究对象,均接受手术治疗,根据手术方式分为腔镜组(20... 详细信息

目的对比观察不同扩张器置入术对颈胸部瘢痕的治疗效果。方法以郑州大学第一附属医院烧伤与修复重建外科2012年3月至2015年8月入院选择扩张器置入手术治疗的23例颈胸部瘢痕患者作为研究对象,均接受手术治疗,根据手术方式分为腔镜组(20个扩张器)、微创组(20个扩张器)、长切口组(20个扩张器),记录3组手术时间、术中出血量、术后引流量、住院时间、术后并发症发生情况、术中注水量和术后首次注水量时间。结果腔镜组手术时间长于微创组和长切口组;腔镜组术中出血量及术后引流量少于微创组与长切口组;腔镜组住院时间短于微创组及长切口组,差异有统计学意义(P0.05)。腔镜组与微创组术中注水量多于长切口组,腔镜组术中注水量多于微创组,差异有统计学意义(P<0.05)。腔镜组与微创组术后首次注水时间短于长切口组,腔镜组术后首次注水时间短于微创组,差异有统计学意义(P<0.05)。结论与传统手术方式比较,腔镜下皮肤软组织扩张器置入手术时间相对较长,但组织损伤小,止血充分,可减少术中及术后出血量,同时腔镜下皮肤软组织扩张器置入可实现术中大量注水及术后及早注水,促进患者术后恢复,缩短住院时间。

关键词：皮肤扩张腔镜微创

A Longitudinal Survey for Genome-based Identification of SARS-CoV-2 in Sewage Water in Selected Lockdown Areas of Lahore City, Pakistan: A Potential Approach for Future Smart Lockdown Strategy

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Biomedical and Environmental Sciences 2021年第9期34卷 729-733页

作者： Tahir Yaqub Muhammad Nawaz Muhammad Z.Shabbir Muhammad A.Ali imran Altaf Sohail Raza Muhammad A.B.Shabbir Muhammad A.Ashraf Syed Z.Aziz Sohai l Q.Cheema Muhammad B.Shah Saira Rafique Sohail Hassan Nageen Sardar Adnan Mehmood Muhammad W.Aziz Sehar Fazal Nadir Hussain Muhammad T.Khan Muhammad M.Atique Ali Asif Muhammad Anwar Nabeel A.Awan Muhammad U.Younis Muhammad A.Bhattee Zarfishan Tahir Nadia Mukhtar Huda Sarwar Maaz S.Rana Omair Farooq Institute of Microbiology University of Veterinary and Animal SciencesLahorePakistan Water and Sanitation Agency LahorePunjabPakistan Specialized Healthcare&Medical Education Department PunjabPakistan Primary and Secondary Healthcare Department PunjabPakistan Institute of Public Health LahorePakistan Farooq Hospital LahorePakistan

In 2019,the newly emerged SARS-CoV-2 virus caused pneumonia-like *** disease rapidly spread globally,leading to a worldwide outbreak referred to as the COVID-19 pandemic.

关键词： globally Strategy Smart

与真菌细胞壁完整性相关的MAP激酶信号途径调控重寄生真菌盾壳霉...

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中国菌物学会第五届会员代表大会暨2011年学术年会

作者：曾凡云 Hamida M.imran 宫晓艳付艳苹谢甲涛程家森李国庆姜道宏农业微生物学国家重点实验室湖北省作物病害监测和安全控制重点实验室华中农业大学植物科学技术学院

盾壳霉（Coniothyrium minitans）是一种腔孢纲真菌,寄生核盘菌（***）菌核和菌核,对由核盘菌引起的作物菌核病具有显著的防治效果,目前,国际上已有以盾壳霉分生孢子为主要有效成份的生防菌剂。研究盾壳霉的分生孢子形成机制,可以提高... 详细信息

盾壳霉（Coniothyrium minitans）是一种腔孢纲真菌,寄生核盘菌（***）菌核和菌核,对由核盘菌引起的作物菌核病具有显著的防治效果,目前,国际上已有以盾壳霉分生孢子为主要有效成份的生防菌剂。研究盾壳霉的分生孢子形成机制,可以提高盾壳霉的生产效率和生防效率。在前期,实验室建立了含45000多个转化子的盾壳霉T-DNA插入体库（Li et al,2005）,并自该插入体库中筛选获得了100多株产孢缺陷型突变体。本文对其中一株突变体（ZS-1T1000）进行了研究,ZS-1T1000不能形成分生孢子原基,在生长后期菌落中央的菌丝塌陷、溃解,寄生菌核的能力显著下降。进一步研究发现,在此突变体中T-DNA插入破坏了一种编码MAP KKK的基因。该基因与酿酒酵母（Saccharomyces cerevisiae）中的BCK1具有较高的同源性,命名为CmBCK1。对CmBCK1进行了定点敲除,发现该基因敲除转化子与ZS-1T1000具有相同的表型。在酿酒酵母中,BCK1调控Slt2,一种与酵母菌细胞壁完整性相关的基因。在盾壳霉基因组也找到了与Slt2类似的基因,命名为CmSlt2-like。对CmSlt2-like进行了敲除,发现该基因的敲除转化子与ZS-1T1000和CmBCK1敲除转化子具有类似的表型,不能产生分生孢子,菌落中央的菌丝也存在塌陷和溃解现象,寄生菌核能力显著下降。研究还表明,与野生菌株ZS-1相比,CmSlt2-like敲除转化子对细胞壁降解酶更加敏感。总之,我们的研究表明与真菌细胞壁完整性相关的MAP激酶信号途径对盾壳霉分生孢子形成至关重要,也对盾壳霉寄生核盘菌具有重要的调控作用。

关键词：盾壳霉重寄生真菌 MAP激酶信号途径分生孢子形成生物防治核盘菌

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区域经济结构转型升级机理分析

Hyoscine for polyp detection during colonoscopy: A meta-analysis and systematic review

区域与城市经济 2017年第8期

作者：安虎森 Muhammad imran

高技能劳动力的转移和空间聚集有助于提升聚集区厂商的创新能力和技术水平，使这些产业部门逐渐发展成为新兴产业部门。新兴产业部门具有较高的技术水平和劳动生产率，且单位土地产值高于传统产业部门。在优势区位竞争中，这些高单位土... 详细信息

高技能劳动力的转移和空间聚集有助于提升聚集区厂商的创新能力和技术水平，使这些产业部门逐渐发展成为新兴产业部门。新兴产业部门具有较高的技术水平和劳动生产率，且单位土地产值高于传统产业部门。在优势区位竞争中，这些高单位土地产值的新兴产业部门将挤走原有的传统产业部门，使原有传统产业部门的生产区位变成高生产率的新兴产业部门的生产区位，被挤出的传统部门则转移到土地价格相对便宜的市郊或边远地区甚至被淘汰。由技术水平提升导致的区域经济总量的增加，意味着区域经济增长；原有低效率的传统产业部门被高效率的新兴产业部门取代，意味着区域经济结构的转型升级；高效率的新兴部门挤出低效率的传统产业部门，意味着产业的区际转移，这就是区域经济结构演进的基本机理。

关键词：创新能力区域经济增长生产区位取代区域经济结构转型升级

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World Journal of Gastrointestinal Endoscopy 2014年第11期6卷 549-554页

作者： imran Ashraf Sohail Ashraf Sameer Siddique Douglas L Nguyen Abhishek Choudhary Matthew L Bechtold Department of Medicine University of Mis-souri Columbia MO 65212 United States Department of Gastroenterology and Hepatology Central Manchester University Hospitals M139WL Manchester United Kingdom Department of Medicine University of California Irvine CA 92868 United States

AIM: To assess the role of hyoscine for polyp detectionduring ***: Studies(randomized controlled trials orRCTs) that compared the use of hyoscine vs no hyo-scine or placebo for polyp detection during colonoscopywere included in our analysis. A search on multiple da-tabases was performed in September 2013 with searchterms being "hyoscine and colonoscopy", "hyoscineand polyp", "hyoscine and adenoma", "antispasmoticand colonoscopy", "antispasmotic and adenoma", and"antispasmotic and polyp". Jadad scoring was used toassess the quality of studies. The efficacy of hyoscinewas analyzed using Mantel-Haenszel model for polypand adenoma detection with odds ratio(OR). The I2measure of inconsistency was used to assess hetero-geneity(P 50%). Statistical analysis was performed by RevMan 5.1. Funnel plots was used to assess publication ***: The search of the electronic databases identified 283 articles. Of these articles, eight published RCTs performed at various locations in Europe, Asia, and Australia were included in our meta-analysis, seven published as manuscripts and one published as an ab-stract(n = 2307). All the studies included patients with a hyoscine and a no hyoscine/placebo group and were of adequate quality(Jadad score ≥ 2). Eight RCTs as-sessed the polyp detection rate(PDR)(n = 2307). The use of hyoscine demonstrated no statistically significant difference as compared to no hyoscine or placebo for PDR(OR = 1.06; 95%CI: 0.89-1.25; P = 0.51). Five RCTs assessed the adenoma detection rate(ADR)(n = 2015). The use of hyoscine demonstrated no statisti-cally significant difference as compared to no hyoscine or placebo for ADR(OR = 1.12; 95%CI: 0.92-1.37; P = 0.25). Furthermore, the timing of hyoscine admin-istration(given at cecal intubation or pre-procedure) demonstrated no differences in PDR compared to no hyoscine or placebo. Publication bias or heterogeneity was not observed for any of the ***: Hyoscine use in pa

关键词： Hyoscine Antispasmodic Polyp detection Colonoscopy

益生菌对肉鸡肠道菌群结构、黏膜免疫功能及TLR信号通路的影响

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益生菌对肉鸡肠道菌群结构、黏膜免疫功能及TLR信号通路的影响

中国畜牧兽医学会动物微生态学分会第十一次全国学术研讨会暨第五届会员代表大会

作者：覃初斌张小平毛予龙李雅丽余东游 imran Rashid Rajput 李卫芬浙江大学动物科学学院饲料研究所动物营养与饲料科学重点实验室

目的动物肠道菌群结构的变化与动物的健康状况息息相关,动物肠道菌群的组成结构可以改善动物肠道的健康、影响动物的营养代谢与吸收、影响动物的生长性能,调节动物的免疫系统等等。我们前期的研究发现布拉氏酵母和枯草芽孢杆菌B10可以... 详细信息

目的动物肠道菌群结构的变化与动物的健康状况息息相关,动物肠道菌群的组成结构可以改善动物肠道的健康、影响动物的营养代谢与吸收、影响动物的生长性能,调节动物的免疫系统等等。我们前期的研究发现布拉氏酵母和枯草芽孢杆菌B10可以促进肉鸡的生长,改善肠道的组织结构(提高肠绒毛的高度和杯状细胞数量,提高肠道紧密连接蛋白occluding,cloudin2及cloudin3 mRNA的表达水平(P<0.05))。本研究以布拉氏酵母和枯草芽孢杆菌B10为饲料添加剂,通过454焦磷酸高通量测序分析它们对肉鸡肠道的菌群结构的影响,以及通过TLR介导的免疫信号通路中相关基因的mRNA表达水平来分析它们对肉鸡肠道免疫功能的影响,为阐明布拉氏酵母和枯草芽孢杆菌B10的益生机制提供一些基础理论。方法试验选用1日龄三黄肉鸡300羽,随机分为三组,每组设5个重复,每个重复20羽。对照组饲喂含抗生素(维吉尼亚霉素,20 mg/kg)的基础日粮,处理组在每千克基础日粮(不添加抗生素)中分别添加1×10 CFU/g饲料的布拉氏酵母(Bs)和枯草芽孢杆菌(Sb)。饲养试验为期72天。结果在空场和回肠中,与对照组相比,布拉氏酵母(Bs)和枯草芽孢杆菌(Sb)处理组可以提高菌群的多样性,特别是在布拉氏酵母(Bs)处理组中。在所有处理组中,厚壁菌门在空肠和回肠中占主导地位,然而在空肠中,布拉氏酵母(Bs)处理组厚壁菌门所占的比例与对照组和枯草芽孢杆菌(Sb)处理组相比显著降低,与此同时,变形菌门(proteobacteria)、疣微菌门(verrucomicrobia)、拟杆菌门(bacteroidetes)及放线菌们(actinobacteria)的比例显著升高。在回肠中,变形菌门(proteobacteria)只有在对照组中出现。此外,布拉氏酵母(Bs)和枯草芽孢杆菌(Sb)处理组的肉鸡空肠黏膜Toll-样受体TLR2、TLR4、TLR15和相关信号蛋白MyD88、TRAF6、TAB2和NF-κB基因表达水平显著上调(P<0.05)。与对照组相比,在空肠和回肠中,益生菌处理组空肠和回肠黏膜中IL-1β、IL-17、IL-4的水平显著提高(P<0.05),然而在空肠黏膜INF-γ、IL-8含量及回肠黏膜INF-γ含量均显著低于对照组(P<0.05)。结论布拉氏酵母菌和枯草芽孢杆菌B10能够通过改善肠道菌群的结构,并通过TLR介导的信号通路有效调节肉鸡肠道黏膜的免疫功能,改善肉鸡肠道的组织结构,促进肉鸡的生长。

关键词：布拉氏酵母菌枯草芽孢杆菌B10 菌群结构免疫调节肠黏膜 Toll-样受体肉鸡

Processed Radio Frequency towards Pancreas Enhancing the Deadly Diabetes Worldwide

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Journal of Endocrinology Research 2021年第1期3卷 1-20页

作者： Md.Rahimullah Miah Mohammad Abdul Hannan AAM Shazzadur Rahman Md.Shahariar Khan Md Mokbul Hossain Ishrat Tasnim Rahman Md.Sabbir Hossain Chowdhury Shadman Shahriar Mohammad Basir Uddin Mohammad Taimur Hossain Talukdar Mohammad Shamsul Alam S.A.M.imran Hossain Alamgir Adil Samdany Shahriar Hussain Chowdhury Alexander Kiew Sayok Department of Health Information Technology Northeast Medical Pvt.LimitedSylhetBangladesh. Department of Endocrinology North East Medical College&HospitalSylhetBangladesh. Department of Medicine North East Medical CollegeSylhetBangladesh. Department of Paediatrics North East Medical College&HospitalSylhetBangladesh. Department of Pharmacology North East Medical College and HospitalSylhetBangladesh. Ananda Niketan SylhetBangladesh. Department of Pathology North East Medical College and HospitalSylhetBangladesh. USA and Ex-student of North East Medical College SylhetBangladesh. Department of Paediatrics North East Medical College and HospitalSylhetBangladesh Department of Clinical Oncology North East Medical College and HospitalSylhetBangladesh. Department of Forensic Medicine North East Medical College and HospitalSylhetBangladesh. Department of Oral and Maxillofacial Surgery North East Medical CollegeSylhetBangladesh. Department of Orthopedics North East Medical CollegeSylhetBangladesh. Department of Dermatology North East Medical CollegeSylhetBangladesh. IBEC University Malaysia SarawakKota SamarahanSarawakMalaysia.

Diabetes is a chronic and debilitating disease,which is associated with a range of complications putting tremendous burden on medical,economic and socio-technological infrastructure *** the higher authorities of health services are facing the excruciating cumulative reasons of diabetes as a very imperative worldwide issue in the 21st *** study aims to relook at the misapplication of the processed radio frequency that frailties in the pancreas within and around the personal body boundary *** administered sensor data were obtained at laboratory experiments from the selected specimens on dogs and cats in light and dark *** study shows the frequent urine flow speed varies with sudden infection due to treated wireless sensor networks in active open *** overweight and obese persons are increasingly affected in diabetes with comprehensive urinary pressure due to continuous staying at dark *** findings replicate the increasing tide of diabetes *** study also represents the difficulties of physicians to provide adequate diabetic management according to their expectancy due to insecure personal area network control *** sensor network is indispensable for healthcare but such network is at risk to health security due to digitalized poisoning within GPS *** study recommends the anti-radiation integrated system policy with user’s security alternative approach to inspire dealing with National Health Policy and Sustainable Development Goals 2030.

关键词： Diabetes Radio frequency Pancreas Active open eyes Body boundary Network security

Efcient Algorithms for Cache-Throughput Analysis in Cellular-D2D 5G Network

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Computers, Materials & Continua 2021年第5期 1759-1780页

作者： Nasreen Anjum Zhaohui Yang imran Khan Mahreen Kiran Falin Wu Khaled Rabie Shikh Muhammad Bahaei Department of Informatics King’s College LondonLondonUK Department of Electrical Engineering University of PeshawarPeshawarPakistan Department of Computer Science Institute of Management SciencesPeshawarPakistan School of Instrumentation and Optoelectronic Engineering Beihang UniversityBeijing100191China Department of Electrical and Electronic Engineering Manchester Metropolitan UniversityManchesterUK

In this paper,we propose a two-tiered segment-based Device-toDevice(S-D2D)caching approach to decrease the startup and playback delay experienced by Video-on-Demand(VoD)users in a cellular *** the S-D2D caching approach cache space of each mobile device is divided into two *** rst cache-block reserve for caching and delivering the beginning portion of the most popular video les and the second cacheblock caches the latter portion of the requested video les‘fully or partially’depending on the users’video watching behaviour and popularity of *** this approach before caching,video is divided and grouped in a sequence of xed-sized fragments called *** control the admission to both cacheblocks and improve the system throughput,we further propose and evaluate three cache admission control *** also propose a video segment access protocol to elaborate on how to cache and share the video segments in a segmentation based D2D caching *** formulate an optimisation problem and nd the optimal cache probability and beginning-segment size that maximise the cache-throughput probability of *** solve the non-convex cache-throughout maximisation problem,we derive an iterative algorithm,where the optimal solution is derived in each *** used extensive simulations to evaluate the performance of our proposed S-D2D caching system.

关键词： Device-to-Device(D2D) startup-delay playback-delay caching