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Antibacterial Activity of Lactic Acid Bacteria and Extraction of Bacteriocin Protein

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Advances in Bioscience and Biotechnology 2020年第2期11卷 49-59页

作者： Rabiul Islam Md. Nur Hossain Md. Khasrul Alam Md. Ekhlas Uddin Mehadi Hasan Rony Md. Abu Sayeed imran Md. Firoz Alam Divisional DNA Screening Laboratory Faridpur Medical College Hospital Ministry of Women & Children Affairs Faridpur Bangladesh Industrial Microbiology Laboratory Institute of Food Science & Technology Bangladesh Council of Scientific & Industrial research Dhaka Bangladesh Department of Biotechnology & Genetic Engineering Islamic University Kushtia Bangladesh Department of Biochemistry and Molecular Biology Gono Bishwabidyalay Dhaka Bangladesh

Biopreservation systems in foods are of increasing interest for industry and consumers. Bacteriocin producing Lactobacillus spp. is considered Generally Recognize as Safe (GRAS), useful to control the fast development of pathogens and spoiling microbes in food and feed. Lactobacillus spp. was isolated from traditional winter fermented vegetable cucumber & carrot by the use of selective media. Especially De Man, Rogosa and Sharpe (MRS) Agar media were used to isolate the Lactobacillus species. Morphologically identified by gram staining & colony morphology. Biochemically recognized by catalase, oxidase, MRVP & carbohydrate fermentation test. Antimicrobial activity of Lactobacillus spp. was confirmed by Well Diffusion Method. Molecular characterization of bacteriocin protein and molecular weight determined by SDS PAGE method. The isolate was found to be facultative anaerobic, Gram positive, and catalase negative. The result of antimicrobial activity measured by the Arbitrary Unit (AU/ml) of zone of the inhibition. Six isolates found from the sample but most activities exhibited isolate 4 against Bacillus megaterium (55 mm) zone of diameter. The molecular weight of the washed bacteriocin was calculated to be about 40 kDa (Isolate 1) and 15 kDa & 30 kDa (Isolate 5). Bacteriocin protein reduces chemical preservatives and uses in future as biopreservative in food industry.

关键词： Lactobacillus spp. Bacteriocin Antimicrobial Activity SDS PAGE Biopreservative

Zinc Chloride Protects against Streptozotocin-Induced Diabetic Nephropathy in Rats

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Pharmacology & Pharmacy 2016年第8期7卷 331-342页

作者： Waleed H. Almalki El-Shaimaa A. Arafa Amal Y. Abdallah Amal M. Mahfoz Afaf O. Osman Hekma A. Abd El-Latif imran Shahid Department of Pharmacology and Toxicology Faculty of Pharmacy Umm Al-Qura University Mecca Saudi Arabia Department of Pharmacology and Toxicology Faculty of Pharmacy Beni-Suef University Beni-Suef Egypt Department of Pharmacology and Toxicology Faculty of Medicine Umm Al-Qura University Mecca Saudi Arabia National Organization for Drug Control and Research (NODCAR) Giza Egypt Department of Biochemistry Faculty of Medicine Umm Al-Qura University Al Qunfudhah Saudi Arabia Department of Pharmacology and Toxicology Faculty of Pharmacy Cairo University Cairo Egypt

Diabetes mellitus (DM) is a health problem affecting millions of individuals worldwide. Diabetic nephropathy (DN), as a significant complication of DM, has become the most common cause of endstage renal failure. Oxidative stress constitutes the key and common events in the pathogenesis of DN and antioxidants may play a beneficial role in its prevention. This study was conducted to investigate the effect of Zinc Chloride on streptozotocin-induced diabetic nephropathy in rats compared to Gliclazide, a reference antidiabetic agent. Results showed that Zinc Chloride was able to control STZ-induced DN in rats as it normalized the elevated blood pressure, the increased insulin release, and the decreased blood glucose level. Zinc Chloride also improved kidney function as determined by the restoration of blood urea and creatinine level. Finally, Zinc Chloride was able to boost the antioxidant defenses of the kidney by increasing the reduced glutathione content and decreasing lipid peroxides content in addition to significantly decreasing kidney nitric oxide content compared to diabetic control rats. These results suggest that exposure to Zinc Chloride can protect from diabetic nephropathy and can be used as an adjuvant approach to treatment and prevention of renal damage.

关键词： Zinc Chloride Oxidative Stress Streptozotocin Diabetes Nephropathy

Anaortic Off-Pump Complete Arterial Revascularization Using Composite LIMA RIMA Y Sequential 5 Grafts in a Patient with Triple Vessel Disease Performed in a Low Resource Country: A Case Report

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World Journal of Cardiovascular Surgery 2021年第12期11卷 125-132页

作者： Asraful Hoque Romena Rahman Abu Shadat Mohammad Saem Khan Md. Abdullah Yusuf Muhammad Asif Ahsan Chowdhury imran Ahmed Wahida Salam Md. Monzur Hossain Tanvir Hossain Department of Cardiac Surgery National Institute of Cardiovascular Diseases Dhaka Bangladesh Department of Microbiology National Institute of Neurosciences and Hospital Dhaka Bangladesh

The use of bilateral internal thoracic arteries to treat coronary artery disease is very less despite of improved long-term survival. In this case report, a patient presented with TVD has been managed with anaortic off-pump complete arterial revascularization (OPCABG) by using composite LIMA RIMA Y Sequential 5 grafts to achieve complete arterial revascularization successfully. This type of operative procedure is technically difficult in a resource poor country like Bangladesh. However, this has been done by a group of young cardiac surgeon. During follow up, the patient is in a good condition.

关键词： Anaortic Off-Pump Complete Arterial Revascularization Composite LIMA RIMA Y Sequential 5 Grafts Triple Vessel Disease Low Resource Country

Role of Hormonal Manipulation in Prostate Cancer Management

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Proceedings of Anticancer Research 2018年第1期2卷 45-53页

作者： Syed imran Ali Shah Central Park Medical College

Prostate cancer(PCa)treatment has seen several important developments over the last few decades in the form of improved surgical methods and advanced radiotherapy techniques but androgen deprivation therapy(ADT)still remains the cornerstone of medical management of this common male *** discovery of androgen-dependent nature of PCa about three-quarters of a century ago was a turning point that has since led to the development of various pharmacological agents which rely on the basic principle of hormonal manipulation in the form of ADT to alter disease *** employed for metastatic disease only,ADT for PCa in the current clinical practice finds use multiple stages of the *** present review summarises the chronological evolution of agents used for hormonal manipulation in the management of PCa,highlighting the pros and cons of each and sheds light on the potential future advances in this area.

关键词： Prostate cancer Androgen deprivation therapy Estrogen Luteinizing hormone releasing hormone

Experience with Post Transplant Parathyroidectomy in Gulf Region and Literature Review

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Open Journal of Nephrology 2013年第4期3卷 211-216页

作者： Samra Abouchacra Ahmed Chaaban Ammar Abdel Baki Khalid Al Mashari Saif Al Sobhi Atia Al Zahrani Qutaiba Hussain Nicole Gebran Mohamed Ahmed imran Khan Bassam Bernieh Mohammad Budruddin Armed Forces Hospital Taif KSA King Faisal Specialist Hospital Research Centre (KFSHRC) Riyadh KSA Sheikh Khalifa Medical City (SKMC) Abu Dhabi UAE Tawam Hospital Al Ain UAE

Sustained elevation of parathyroid hormone (PTH) levels is not uncommon post renal transplantation. Though in the majority of patients, it gradually normalizes, on average 5% of transplanted patients require parathyroidectomy (PTX). However, PTX itself has been associated with deterioration in allograft function and even completes graft loss seen with both total and subtotal PTX as well as an increased rate of acute rejection. The aim of this study was to determine the effect of post transplant partial PTX on allograft function in our patients as well as the incidence of acute rejection. Our results show that post transplantation, subtotal PTX, was successful in achieving metabolic control while preserving graft function without an increased incidence of acute rejection. Retention of sufficient residual parathyroid tissue with partial PTX might account for favorable outcome in our study. Despite this, surgery for advanced hyperparathyroidism should optimally take place in the pre-transplant period.

关键词： Parathyroidectomy Renal Transplantation Parathyroid Hormone Hyperparathyroidism

Primitive neuroectodermal tumor of uterus——A rare entity

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The Chinese-German Journal of Clinical Oncology 2010年第6期9卷 365-367页

作者： Syed Nisar Ahmad Shiekh Aejaz Aziz Rangreze imran Manzoor Ahmad Banday Gul Mohammad A.R.Lone Parveen Reshi Nisar Ahmad Wani Shaheena Parveen Department of Medical Oncology SKIMS SouraSrinagar 190011India Department of Gynae/obstretics SKIMS SouraSrinagar 190011India Department of Radiodiagnosis SKIMS SouraSrinagar 190011India Department of Gastroenterology SKIMS SouraSrinagar 190011India

Primitive neuroectodermal tumor (PNET) of uterus is the rare of the rarest among the tumors of female genital tract. We herein reported a 32 years old female operated for lower abdominal lump,which was diagnosed,as PNET later confirmed by immunohistochemistry (IHC). She was lost to follow-up and presented 10 months later with recurrence. She was treated with cyclical combination chemotherapy followed by definitive surgery and is in complete remission at present.

关键词： neuroectodermal tumor uterus

Automated Equipment for Characterization of Web Media （COF and Stiffness Measurement）

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Automated Equipment for Characterization of Web Media （COF ...

Evaluation of Different Radish (Raphanus sativus) Genotypes under Different Saline Regimes

2012 International Conference on Industrial and Intelligent Information(ICIII 2012)

作者： Santhosh.S Tarun Teja.M G.Sriram I.Yousuf imran Ali C.Elanchezhian B.Vijaya Ramnath Bachelors' in Mechanical Engineering Sri Sairam Engineering CollegeAnna University-Chennai Professor Department of Mechanical EngineeringSri Sairam Engineering CollegeAnna University-Chennai

The term "WEB" refers to all materials pertaining to micron-level thickness in industrial *** web media play a dominant role in the printing industries,chemical industries,manufacturing industries and automobile industries *** term "Characterization" refers to all the parameters that define a web *** inevitable characteristics of web media include Co-efficient of friction,Stiffness of media,Absorbing capacity of media,tensile and compressive strength of media *** these characteristics,Co-efficient of friction and Stiffness of web media influence an inevitable impact in designing printing machines,textile industry,chemical filtration and refinery *** project is aimed towards designing a compact-portable instrument that readily portrays the Static and Dynamic Co-efficient of Friction between any two web media and stiffness of the particular web media under *** instrument is fully automated and gives the output in digital *** this instrument,the industries relying on web media will be benefitted to a great extent.

关键词： Web characterization co-efficient of friction stiffness

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American Journal of Plant Sciences 2016年第6期7卷 894-898页

作者： C. M. Ayyub Muhammad Rashid Shaheen Samad Raza Muhammad Sarwar Yaqoob Rashad Waseem Khan Qadri Muhammad Azam M. Awais Ghani imran Khan Naheed Akhtar Institute of Horticultural Sciences University of Agriculture Faisalabad Pakistan College of Agriculture and Environmental Sciences TheIslamia University of Bahawalpur Punjab Pakistan Department of Agronomy University of Agriculture Faisalabad Pakistan College of Agriculture Dera Ghazi Khan Pakistan

An effective and simple screening technique for identification of salt tolerant and salt sensitive radish genotypes was observed. Sand is used as potting media. Six genotypes of radish were used for screening against four salinity levels (0, 1, 3, 5 and 7 dS/m-1). Twenty days old seedlings of radish were salinized with salt solution (NaCl). Morphological, physiological and ionic parameters were studied. Radish genotypes Laal-Pari and 40 Days executed the best performance in all the measured attributes and categorized as salt tolerant genotype while Green Neck was the poorest in retaining normal functioning at higher salinity levels thus grouped under salt sensitive cultivar.

关键词： Radish Salinity Physiological Morphological Ionic Attributes Genotypes

Gene Action Studies in the Inheritance of Economic Traits in Diallel Cross of Lentil (Lens culinaris Medik)

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American Journal of Plant Sciences 2016年第6期7卷 756-768页

作者： Fahim Abbas Asim Riaz Tanzeela Zafar Muhammad Hassan Hafiz Muhammad imran Umar Javed Hassan Waseem Alam Saleh Muhammad Mudassir Mahmood Hamza Sohail Usman Ali Tiwana Muhammad Tayyab Iqbal Muhammad Bilal Nanjing Agriculture University Nanjing China Department of Pharmacy Health and Well-Being University of Sunderland Sunderland UK Centre of Agricultural Biochemistry and Biotechnology Faculty of Agriculture University of Agriculture Faisalabad Pakistan Department of Plant Breeding & Genetics Bahauddin Zakariya University Multan Pakistan Department of Entomology Bahauddin Zakariya University Multan Pakistan Department of Horticulture Bahauddin Zakariya University Multan Pakistan Arid Zone Research Institute Bahawalpur Pakistan Department of Plant Breeding & Genetics PMAS-UAA Rawalpindi Pakistan

The present study was conducted in 4 × 4 half diallel cross fashion using the genotypes named as NL-2002, NL-2006, Punjab Masoor-2009 and Markaz-2009 for the evaluation of gene action in the inheritance of economic traits of lentil by following the Griffing (1956) Method of analysis. Minirap 13.1 Copyright© 2000, Minitab inc. Method II, Model I was used for the analysis and gene action was computed by variance component analysis using Mixed Model with GLM (general linear model) procedure. The F1 direct crosses along with the parental genotypes were then evaluated by framing the experiment in RCBD in 3 replications. The data were recorded for the characters which are under the study are, plant height (cm), number of primary branches, number of secondary branches, number of pods per plant, number of seed per pod, seed yield per plant, biomass (gram), 100 seeds weight (gram), harvest index (%), yield per plant. The simple ANOVA for all the characters revealed that difference among genotypes was highly significant. As significant differences were present between the genotypes, it permitted subsequent analysis of the data. The Combining Ability (CA) results showed that value of variance for GCA (General Combining Ability) was significantly higher than that of those of the values of variance of SCA (Specific Combining Ability). Hence current results clearly depicted the role of additive type of gene action governing the characters under study.

关键词： Lentil Diallel Gene Action Studies