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Fungal diversity in adult date palm(Phoenix dactylifera L.) revealed by culture-dependent and culture-independent approaches

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2013年第12期14卷 1084-1099页

作者： Ines BEN CHOBBA Amine ELLEUCH imen ayadi Lamia KHANNOUS Ahmed NAMSI Frederique CERQUEIRA Noureddine DRIRA Néji GHARSALLAH Tatiana VALLAEYS Laboratoire de Biotechnologies Végétales Appliquées à l'Amélioration des Cultures LBVAAC Faculté des Sciences de SfaxB.P. 1171 3000 Sfax Tunisia UMR 5119 Université de Montpellier 2 Sciences et Techniques pl E. BataillonCC093 34095 Montpellier France Laboratoire de Microorganismes et de Biomolécules Centre de Biotechnologie de Sfax Université de Sfax Route Sidi MansourB.P. 1177 3018 Sfax Tunisia Laboratoire de Phytopathologie Oasienne Centre Régional de Recherches en Agriculture Oasienne à DegacheDegache 2260 Tunisia Plateforme Séquen age-Génotypage SFR "Montpellier Environnement Biodiversité" ISEM Université de Montpellier 234095 Montpellier France

Endophytic flora plays a vital role in the colonization and survival of host plants, especially in harsh environments, such as arid regions. This flora may, however, contain pathogenic species responsible for various troublesome host diseases. The present study is aimed at investigating the diversity of both cultivable and non-cultivable endophytic fungal floras in the internal tissues(roots and leaves) of Tunisian date palm trees(Phoenix dactylifera). Accordingly, 13 isolates from both root and leaf samples, exhibiting distinct colony morphology, were selected from potato dextrose agar(PDA) medium and identified by a sequence match search wherein their 18S–28S internal transcribed spacer(ITS) sequences were compared to those available in public databases. These findings revealed that the cultivable root and leaf isolates fell into two groups, namely Nectriaceae and Pleosporaceae. Additionally, total DNA from palm roots and leaves was further extracted and ITS fragments were amplified. Restriction fragment length polymorphism(RFLP) analysis of the ITS from 200 fungal clones(leaves: 100; roots: 100) using HaeIII restriction enzyme revealed 13 distinct patterns that were further sequenced and led to the identification of Alternaria, Cladosporium, Davidiella(Cladosporium teleomorph), Pythium, Curvularia, and uncharacterized fungal endophytes. Both approaches confirmed that while the roots were predominantly colonized by Fusaria(members of the Nectriaceae family), the leaves were essentially colonized by Alternaria(members of the Pleosporaceae family). Overall, the findings of the present study constitute, to the authors' knowledge, the first extensive report on the diversity of endophytic fungal flora associated with date palm trees(P. dactylifera).

关键词： Date palm tree(Phoenix dactylifera) Endophytic cultivable fungi rDNA internal transcribed spacer(ITS) Phylogenetic analysis Total DNA diversity analysis

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Impact du traitement carcinologique sur le fonctionnement sexuel et l’image du corps des patientes tunisiennes en rémission d’un cancer du sein localiséImpact of Cancer Treatment on Sexual Functioning and Body Image of Tunisian Patients in Remission from Localized Breast Cancer

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Psycho-Oncologie 2024年第3期18卷 241-248页

作者： Nada Charfi Nour Regaieg Ines ayadi Najeh Smaoui Rim Feki imen Gassara Sana Omri Lobna Zouari Jihène Ben Thabet Manel Maâlej Bouali Afef Khanfir Mohamed Maâlej Service de psychiatrie C CHU Hedi ChakerSfax3000Tunisie Service de carcinologie médicale CHU Habib BourguibaSfax3029Tunisie Facultéde médecine de Sfax Universitéde SfaxSfax3029Tunisie

The diagnosis and the management of breast cancer(BC)affect in a complex way women’s sexual lives and touch the symbol of femininity,which is very important in the constitution of the body *** objectives were to assess the sexual functioning and the body image of patients in remission of localized BC,to determine the links between these two parameters,and to study their relationship with the therapeutic *** conducted a cross-sectional,descriptive,and analytical study in the medical oncology and radiotherapy departments of Habib Bourguiba University Hospital Center in Sfax(Tunisia),between February and August *** were assessed based on a clinical information sheet and two psychometric scales exploring sexual functioning and body image:Female Sexual Function Index(FSFI)and Body Image Scale(BIS).The sample comprised 74 patients with a mean age of 51.55 *** were treated by conservative or radical surgery(44.6%and 55.4%,respectively),chemotherapy(87.8%),hormone therapy(79.7%),targeted therapies(36.5%),and radiotherapy(97.3%).A rate of 51.4%of women had sexual dysfunction(SD),87.8%had hypoactive sexual desire and 36.5%had body image *** body image disorder was significantly associated with DS(p=0.001).The total FSFI score(p=0.001)as well as the subscores of desire(p=0.005),arousal(p=0.003),orgasm(p=0.002),satisfaction(p<0.001),and pain(0.03)were significantly lower in patients with body image *** presence of a SD was not associated with any therapeutic *** contrast,the presence of body image disorder was significantly associated with the radical type of surgery(p=0.003)and chemotherapy(p=0.001).Our results showed a relatively high frequency of SD and body image disorder in women in remission from *** two parameters seem to be closely linked,and this link would be maintained by mastectomy and chemotherapy,which appear to be therapeutic modalities incriminated in the disturbance of body *** the importan

关键词： Breast cancer sexual dysfunction body image Female Sexual Function Index Body Image Scale

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Mature Teratoma Revealed by an Encysted Pleural Effusion

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Open Journal of Respiratory Diseases 2021年第2期11卷 84-89页

作者： Houda Gharsalli Monia Attia Sarra Zairi imen Sahnoun Aida ayadi Henda Neji Sonia Maalej Leila Douik ElGharbi Department of Pulmonology Abderrahmane Mami Hospital Ariana Tunisia University of Tunis El Manar Tunis Tunisia Department of Radiology Abderrahmane Mami Hospital Ariana Tunisia Department of Thoracic and Cardiovascular Surgery Abderrahmane Mami Hospital Ariana Tunisia Department of Pathology Abderrahmane Mami Hospital Ariana Tunisia

A 32-year-old patient with no previous history was admitted for chest pain, dyspnea, appetite and weight loss. Chest X-ray revealed an opacity involving the lower two-thirds of the right hemithorax, suggestive of a pleural effusion. Because of the absence of fluid return even after ultrasound-guided thoracentesis, a Chest Computed tomography was required showing a heterogeneous anterior mediastinal mass with soft tissue, fat, fluid and calcifications associated with extensive encysted fluid collection in the right hemithorax. A video-assisted mini-thoracotomy revealed a mediastinal tumor firmly attached to the thymus with a cystic wall lined by squamous epithelium and sebaceous gland composed of respiratory tissue, adipose tissue, cartilage,

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