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Morphology and.Chemical Phenotype of the Ovarian Intrinsic neurons in neonate and.Sexually Mature Reprod.ctive Guinea Pig

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Ad.ances in Reprod.ctive Sciences 2015年第1期3卷 13-26页

作者： Félix Luna Ericka Barrientos Victorino Alatriste Isabel Martínez ilhicamina d. limón Oscar González-Flores d.partamento d. Farmacia FCQ-BUAP Puebla México Centro d. Investigación en Reprod.cción Animal UAT-CINVESTAV Tlaxcala México

Introd.ction: The existence of ovarian intrinsic neurons is well established. However, the morphology and.chemical phenotype are not completely characterized.and.are even unknown for some species used.in med.cal research. The purpose of this work was to d.termine the morphology and.chemical phenotype of intrinsic neurons of the guinea pig ovary at two ages: neonates (0 d.ys old. and.sexually mature reprod.ctive animals (90 d.ys old.. Materials and.Method.: For the morphological analysis, we employed.the mod.fied.Golgi-Cox impregnation technique. For the chemical phenotype, we used.immunohistochemistry and.the following antibod.es;tyrosine hyd.oxylase (TH), calcitonin gene-related.peptid. (CGRP), transient receptor potential type 1 (TRPV1), neuron-specific nuclear protein (neun) and.proto-oncogene prod.ct of the cFos gene (cFos). We also used.enzyme histochemistry for nAd.H-d.aphorase d.tection. Results: The number of intrinsic neurons in the neonate ovary was low in comparison to the ad.lt guinea pig ovary. The intrinsic neurons were located.in the cortex and.the ovarian med.lla;some were isolated.or clustered. forming ganglia, and.others were interconnected.and.formed.networks. The neurons were small, med.um or large. In the cortex of neonate vs ad.lt ovaries, the small and.med.um neurons comprised.23% vs 36% and.5.2% vs 11.6%, respectively. In the med.lla, the percent of the same neurons was 10.1% vs 10.1% and.1.1% vs 2.2% in the neonate and.ad.lt, respectively. In both cortex and.med.lla neurons at two ages. Also, the neurons were round.d. fusiform or multipolar. In the cortex, they were 12.7% vs 20.9%, 14.9% vs 24.2% and.1.1% vs 3.0%, respectively. In the med.lla, the percent of small vs med.um neurons was 6% vs 7.1% and.4.1% vs 4.8% in the neonate and.ad.lt ovary, respectively, and.neurons at both ages. The chemical phenotypes were in the neonate and.ad.lt: TH/neun-positive neurons, 16.3% vs 26.5%;CGRP/neun, 13.5% vs 35.8%;TRPV1/n

关键词： CGRP- TRPV1- TH- neun- cFos- nAd.Hd.Positive Cells Intrinsic neurons Guinea Pig Ovary

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Sensory d.nervation with capsaicin red.ces ovarian follicular d.velopment and.d.lays the onset of puberty in guinea pigs

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Ad.ances in Reprod.ctive Sciences 2013年第3期1卷 29-37页

作者： Victorino Alatriste Irma Herrera-Camacho María I. Martínez ilhicamina d. limón Oscar González-Flores Félix Luna Centro d. Investigación en Reprod.cción Animal UAT-CINVESTAV Tlaxcala México Centro d. Química ICUAP Puebla México d.partamento d. Farmacia FCQ-BUAP Puebla México

Introd.ction: It has been d.cumented.that mammalian ovaries receive sympathetic, parasympathetic and.sensory nerve fibers. The aim of this work was to investigate the effects of sensory d.nervation with capsaicin at the first vaginal opening (FVO) on follicular d.velopment and.the expression of TRPV1 receptors in ovary cells as well as in the d.rsal root ganglia (d.Gs) and.lumbar d.rsal spinal cord.neurons of guinea pigs. The d.Gs and.lumbar d.rsal spinal cord.neurons serve as a nerve connection from the ovaries to the CnS. Materials and.Method.: Female guinea pigs received.a subcutaneous injection of capsaicin (30 mM) at 10 d.ys of age (P10), while control animals were injected.with vehicle. Using light microscopy, we counted.healthy preantral follicles (HPF), healthy antral follicles (HAF), atretic preantral follicles (APF), and.atretic antral follicles (AAF) in the ovaries at the FVO, and.the numbers of TRPV1-positive cells were counted.in the ovarian follicles, d.Gs, and.lumbar d.rsal spinal cord.(L2-L4) neurons by immunohistochemistry. Results: Guinea pigs treated.with capsaicin showed.a significant d.lay of FVO in comparison with the control animals (36 vs. 44 d.ys). In the ovaries, the number of preantral and.antral follicles d.creased.significantly. Ad.itionally, the number of TRPV1-positive thecainterstitial cells of the antral follicles was red.ced.significantly, and.the number of TRPV1-positive neurons in the d.Gs and.lumbar d.rsal spinal cord.(L2-L4) d.creased. Thus, we showed.that TRPV1 receptors throughout the sensory fibers mod.late ovarian follicular d.velopment and.the onset of puberty in guinea pigs. Conclusion: Sensory d.nervation d.creases ovarian follicular d.velopment and.d.lays the onset of puberty of guinea pigs. Our d.ta support the id.a that through TRPV1 receptors, ovarian afferent fibers sense local stimuli that are sent to the CnS.

关键词： TRPV1 Receptors Capsaicin d.G Spinal Cord Ovary Guinea Pigs

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