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Purification and characterization of glyceraldehyde-3-phosphate dehydrogenase from saline strain Idiomarina loihiensis

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Advances in Biological Chemistry 2013年第2期3卷 170-176页

作者： ilham mardad Tarik Baibai Emna Ammar Abdelaziz Soukri Laboratoire de Physiologie et Génétique Moléculaire Facultédes Sciences Ain ChockUniversitéHassan II-Ain ChockCasablancaMorocco URétude et Gestion des Environnements Urbains et Cotiers LARSENUniversitéde Sfaxécole Nationale d’Ingénieurs de SfaxTunisia

Idiomarina loihiensis was isolated from the salt works in Sfax (Tunisia), until now, the characterization of the GAPDH phosphorylante was never studied. Here, we report the isolation and the biochemical characterization of glyceralehyde-3-phosphate dehydrogenase (GAPDH) fromI. loihiensis saline’s bacteria on the basis of the apparent native and subunit molecular weights, physico-chemical and kinetic characterizations. The purification method consisted of two steps, ammonium sulfate fractionation followed by one chromatographic step, namely dye-affinity on Blue Sepharose CL-6B. Polyclonal antibodies against the purified enzyme were used to recognize theI. loihiensis GAPDH by Western blotting. The optimum pH of the purified enzyme was 8.5. Studies on the effect of temperatures revealed an enzyme increasing activity of about 45?C. The molecular weight of the purified enzyme was 36 kDa determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Non-denaturing polyacrylamide gels yield a molecular weight of 147 kDa. The Michaelis constants for NAD+ and D-glyceraldehyde-3-phosphate estimated was 19 μM and 3.1 μM, respectively. The maximal velocity of the purified enzyme was estimated to be 2.06 U/mg, approximately 6-fold increase in specific activity and a final yield of approximately 32.5%. The physicochemical properties of this GAPDH, being characterized, could be used in further studies.

关键词： Glyceraldehyde-3-Phosphate Dehydrogenase Idiomarina loihiensis Purification NAD^(+) Kinetics Saline Strain

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Enhanced asphalt dynamic modulus prediction: A detailed analysis of artificial hummingbird algorithm-optimised boosted trees

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Journal of Road Engineering 2024年第2期4卷 224-233页

作者： Ikenna D.Uwanuakwa ilham Yahya Amir Lyce Ndolo Umba Department of Civil Engineering Near East UniversityMersin 10Turkey Faculty of Sciences University of LubumbashiLubumbashiThe Democratic Republic of Congo

This study introduces and evaluates a novel artificial hummingbird algorithm-optimised boosted tree(AHAboosted)model for predicting the dynamic modulus(E*)of hot mix asphalt *** a substantial dataset from NCHRP Report-547,the model was trained and rigorously *** metrics,specifically RMSE,MAE,and R2,were employed to assess the model's predictive accuracy,robustness,and *** benchmarked against well-established models like support vector machines(SVM)and gaussian process regression(GPR),the AHA-boosted model demonstrated enhanced *** achieved R2 values of 0.997 in training and 0.974 in testing,using the traditional Witczak NCHRP 1-40D model *** features such as test temperature,frequency,and asphalt content led to a 1.23%increase in the test R2,signifying an improvement in the model's *** study also explored feature importance and sensitivity through SHAP and permutation importance plots,highlighting binder complex modulus|G*|as a key *** the AHA-boosted model shows promise,a slight decrease in R2 from training to testing indicates a need for further ***,this study confirms the AHA-boosted model as a highly accurate and robust tool for predicting the dynamic modulus of hot mix asphalt concrete,making it a valuable asset for pavement engineering.

关键词： Asphalt Dynamic modulus Prediction Artificial hummingbird algorithm Boosted tree

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Assessing Moroccan physician knowledge and practices regarding maternal obesity's impact on childhood obesity:Implications for prevention and intervention

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World Journal of Clinical Pediatrics 2024年第2期13卷 146-160页

作者： Imane Douiyeh Jihane Khamlich Naima Nabih Asmae Saih ilham Boumendil Anas Regragui Anass Kettani Amal Safi Laboratory Biochemistry Environment and Agri-food Department of BiologyFaculty of Science and Technics MohammediaHassanⅡUniversity CasablancaMohammedia 28806Morocco Department of Biology Faculty of Sciences Ben M’Sik HassanⅡUniversity of CasablancaCasablanca 20000Morocco Department of Medical ACHAS Association of the Doctors Ain Chock Casablanca MoroccoCasablanca 20400Morocco Department of Medical HassanⅡUniversityCasablanca 20400Morocco

BACKGROUND Childhood obesity is a growing global concern with far-reaching health *** study focuses on evaluating the knowledge and practices of physicians in Morocco regarding the link between maternal obesity and childhood *** the increasing prevalence of childhood obesity worldwide,this issue remains inadequately addressed in the Moroccan *** To assess the awareness and practices of physicians in Morocco concerning the connection between maternal obesity and childhood *** The research encompasses a comprehensive survey of practicing physicians,revealing significant gaps in awareness and practices related to maternal *** Notably,a significant portion of doctors do not provide adequate guidance to overweight pregnant women,highlighting the urgency for targeted educational *** In conclusion,this research illuminates critical areas for improvement in tackling childhood obesity in *** addressing these gaps,fostering awareness,and enhancing medical practices,the healthcare system can contribute significantly to preventing childhood obesity and improving the overall health of future generations.

关键词： Childhood obesity Maternal obesity Management Prevention Screening

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聚合物基抗生素缓释载体治疗慢性骨髓炎

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中国组织工程研究 2024年第22期28卷 3597-3602页

作者：伊力哈木·麦麦提阿卜杜拉黄晓夏李璐遥滕勇新疆医科大学研究生学院中国人民解放军新疆军区总医院

背景：慢性骨髓炎的治疗策略中，局部抗生素缓释系统因可长期释放有效浓度的抗生素控制感染，同时能修复彻底清创后引起的骨缺损在临床中备受关注。目的：总结可生物降解聚合物基材料制备抗生素缓释载体用于治疗骨髓炎的研究现状，并分... 详细信息

背景：慢性骨髓炎的治疗策略中，局部抗生素缓释系统因可长期释放有效浓度的抗生素控制感染，同时能修复彻底清创后引起的骨缺损在临床中备受关注。目的：总结可生物降解聚合物基材料制备抗生素缓释载体用于治疗骨髓炎的研究现状，并分析局限性及挑战。方法：作者以“Polymer,Composite material,Osteomyelitis,Infectious bone defect,Drug delivery systems,Antibiotic sustained-release system,3D printing；聚合物，复合材料，骨髓炎，感染性骨缺损，药物递送系统，抗生素缓释系统，3D打印”为关键词，检索2015年1月至2023年8月期间Pub Med、Web of Science、中国知网、万方数据库中的相关文献。初检得到文章4 351篇，筛选后对87篇文章进行分析。结果与结论：聚合物基材料因具有良好的生物相容性、生物降解性能、热稳定性及易加工性等多功能特点在抗生素缓释载体制备中得到广泛研究，但单一聚合物材料组成的抗生素缓释载体因生物力学性能不足等原因无法满足感染性骨缺损修复材料的标准。模拟天然骨组织结构形成的有机-无机复合材料型载体有望达到这个标准。3D打印技术可以精确控制载体的相互连接孔隙大小、几何形状和空间分布等，并能负载有效浓度的抗生素做到控制释放。高分子聚合物材料因具有良好的热稳定性、可塑性等优点最适合用于3D打印。因此在新型可生物降解有机-无机复合材料基础上，结合3D打印技术建立材料-结构-功能一体化的复合抗生素缓释载体因仿真模拟细胞外基质微环境有望成为慢性骨髓炎治疗中新颖的研究方向。

关键词：聚合物复合材料骨髓炎感染性骨缺损药物递送系统抗生素缓释系统 3D打印

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A Review on Active Customers'Participation in Smart Grids

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Journal of Modern Power Systems and Clean Energy 2023年第1期11卷 3-16页

作者： Konrad Schmitt Rabindra Bhatta Manohar Chamana Mahtab Murshed ilham Osman Stephen Bayne Luciane Canha Electrical and Computer Engineering Department Texas Tech UniversityLubbockTX79409USA Wind Energy Program Texas Tech UniversityLubbockTX79409USA Electromechanical and Power Systems Department Federal University of Santa MariaSanta MariaRS97105-900Brazil Design Release Engineer at Electrification and Power conversion General MotorsGM Tech Center RoadWarrenMI48092USAl IEEE

Industrial,commercial,and residential facilities are progressively adopting automation and generation *** having flexible demand and renewable energy generation,traditional passive customers are becoming active participants in electric power system *** profound coordination among grid operators and active customers,the facilities’capability for demand response(DR)and distributed energy resource(DER)management will be valuable asset for ancillary services(ASs).To comply with the increasing demand and flexible energy,utilities urgently require standards,regulations,and programs to efficiently handle load-side resources without trading off stability and *** study reviews different types of customers’flexibilities for DR,highlighting their capabilities and limitations in performing local ancillary services(LASs),which should benefit the power grid by profiting from it through incentive *** financial incentives and techniques employed around the world are presented and *** potential barriers in technical and regulatory aspects are successfully identified and potential solutions along with future guidance are discussed.

关键词： Active distribution network(ADN) ancillary services energy management flexible demand resiliency

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