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Neural Regeneration Research 2015年第2期10卷 213-218页

作者： ilaria dal prà Anna Chiarini Ubaldo Armato Histology & Embryology Section Department of Life & Reproduction Sciences University of Verona Medical School

Astrocytes＇ roles in late-onset Alzheimer＇s disease （LOAD） promotion are important, since they survive soluble or fibrillar amyloid-β peptides （Aβs） neurotoxic effects, undergo alterations of intracellular and intercellular Ca2＋ signaling and gliotransmitters release via the Aβ/a7-nAChR （αT-nicotinic acetylcholine receptor） signaling, and overproduce/oversecrete newly synthesized Aβ42 oligomers, NO, and VEGF-A via the Aβ/CaSR （calcium-sensing receptor） signaling. Recently, it was suggested that the NMDAR （N-methyl-D-aspartate receptor） inhibitor nitromemantine would block the synapse-destroying effects of Aβ/α7-nAChR signaling. Yet, this and the progressive extracellular accrual and spreading of Aβ42 oligomers would be stopped well upstream by NPS 2143, an allosteric CaSR antagonist （calcilytic）.

关键词： Alzheimer's disease amyloid-β astrocytes Ca2+ calcilytic calcium-sensing receptor nitromemantine NPS 2143 aT-nicotinic acetylcholine receptor

Exosomes of adult human fibroblasts cultured on 3D silk fibroin nonwovens intensely stimulate neoangiogenesis

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Burns & Trauma 2021年第1期9卷 488-506页

作者： Peng Hu Anna Chiarini Jun Wu Giuliano Freddi Kaiyu Nie Ubaldo Armato ilaria dal prà Human Histology&Embryology Section Department of SurgeryDentistryPaediatrics&GynaecologyUniversity of Verona Medical SchoolStrada Le Grazie 8I-37134VeronaVenetiaItaly Department of Burns&Plastic Surgery The Affiliated Hospital of ZunYi Medical University149 Dalian RoadZunYi City563003 Guizhou ProvinceChina Department of Burns and Plastic Surgery Second People’s HospitalUniversity of Shenzhen3002 Sungang West RoadFutian DistrictShenzhen518000Guangdong ProvinceChina Silk Biomaterials S.r.l. Via Cavour 2I-22074LomazzoLombardyItaly

Background:Bombyx mori silk fibroin is a biomacromolecule that allows the assembly of scaffolds for tissue engineering and regeneration purposes due to its cellular adhesiveness,high biocompatibility and low *** work showed that two types of 3D silk fibroin nonwovens(3D-SFnws)implanted into mouse subcutaneous tissue were promptly vascularized via undefined molecular *** present study used nontumorigenic adult human dermal fibroblasts(HDFs)adhering to a third type of 3D-SFnws to assess whether HDFs release exosomes whose contents promote ***:Electron microscopy imaging and physical tests defined the features of the novel carded/hydroentangled *** were cultured on 3D-SFnws and polystyrene plates in an exosome-depleted *** amounts and D-glucose consumption revealed the growth and metabolic activities of HDFs on ***9-expressing total exosome fractions were from conditioned media of 3D-SFnws and 2D polystyrene plates HDF *** growth factors(AGFs)in equal amounts of the two groups of exosomal proteins were analysed via doubleantibody arrays.A tube formation assay using human dermal microvascular endothelial cells(HDMVECs)was used to evaluate the exosomes’angiogenic ***:The novel features of the 3D-SFnws met the biomechanical requirements typical of human soft *** experimental day 15,3D-SFnws-adhering HDFs had increased 4.5-fold in numbers and metabolized 5.4-fold more D-glucose than at day 3 in *** to polystyrene-stuck HDFs,exosomes from 3D-SFnws-adhering HDFs carried significantly higher amounts of AGFs,such as interleukin(IL)-1α,IL-4 and IL-8;angiopoietin-1 and angiopoietin-2;angiopoietin-1 receptor(or Tie-2);growth-regulated oncogene(GRO)-α,GRO-βand GRO-γ;matrix metalloproteinase-1;tissue inhibitor metalloproteinase-1;and urokinase-type plasminogen activator surface receptor,but lesser amounts of anti-angiogenic tissue inhibitor metalloproteinase-2 and

关键词： Silk fibroin Nonwovens Dermis Fibroblast Human endothelial cell Exosome Cytokine Chemokine Angiogenesis Regeneration Tissue engineering

Mesenchymal stromal cells-exosomes:a promising cell-free therapeutic tool for wound healing and cutaneous regeneration

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Burns & Trauma 2019年第1期7卷 346-355页

作者： Peng Hu Qinxin Yang Qi Wang Chenshuo Shi dali Wang Ubaldo Armato ilaria dal prà Anna Chiarini Department of Burns&Plastic Surgery The Affiliated Hospital of ZunYi Medical UniversityDalian Road 149ZunYi City 563000Gui Zhou ProvinceChina Human Histology and Embryology Unit University of Verona Medical SchoolStrada Le Grazie 837134 VeronaItaly

Cutaneous regeneration at the wound site involves several intricate and dynamic processes which require a series of coordinated interactions implicating various cell types,growth factors,extracellular matrix(ECM),nerves,and blood *** stromal cells(MSCs)take part in all the skin wound healing stages playing active and beneficial roles in animal models and ***,which are among the key products MSCs release,mimic the effects of parental *** can shuttle various effector proteins,messenger RNA(mRNA)and microRNAs(miRNAs)to modulate the activity of recipient cells,playing important roles in wound ***,using exosomes avoids many risks associated with cell ***,as a novel type of cell-free therapy,MSC-exosome-mediated administration may be safer and more efficient than whole *** this review,we provide a comprehensive understanding of the latest studies and observations on the role of MSC-exosome therapy in wound healing and cutaneous *** addition,we address the hypothesis of MSCs microenvironment extracellular vesicles(MSCs-MEVs)or MSCs microenvironment exosomes(MSCs-MExos)that need to take stock of and solved urgently in the related research about MSC-exosomes therapeutic *** review can inspire investigators to explore new research directions of MSC-exosome therapy in cutaneous repair and regeneration.

关键词： Cutaneous regeneration Mesenchymal stromal cell Exosomes Wound healing Microenvironment

A novel method for objectively, rapidly and accurately evaluating burn depth via near infrared spectroscopy

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Burns & Trauma 2021年第1期9卷 393-400页

作者： Meifang Yin Yongming Li Yongquan Luo Mingzhou Yuan Ubaldo Armato ilaria dal prà Lijun Zhang Dayong Zhang Yating Wei Guang Yang Lixian Huang Pin Wang Jun Wu Department of Burn and Plastic Surgery Institute Translational MedicineThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People’s HospitalShenzhen 518035China School of Microelectronics and Communication Engineering Chongqing UniversityChongqing 400044China Institute of Fluid Physics China Academy of Engineering PhysicsMianyangSichuan 621900China Department of Burn Surgery The First Affiliated HospitalSun Yat-sen UniversityGuangzhou 510080China Human Histology&Embryology Section Department of SurgeryDentistryPediatrics&GynecologyUniversity of Verona Medical SchoolStrada Le Grazie 8Verona 37134Italy

The accurate and objective evaluation of burn depth is a significant challenge in burn wound ***,we used near infrared spectroscopy(NIRS)technology to measure the different depth of thermal burns in ex vivo porcine *** on the intensity of the spectral signals and the diffuse reflection theory,we extracted the optical parameters involved in functional(total hemoglobin andwater content)and structural(tissue scattered size and scattered particles)features that reflect the changes in burn ***,we applied support vector regression to construct a model including the optical property parameters and the burn ***,we histologically verified the burn depth data collected via *** results showed that our inversion model could achieve an average relative error of about 7.63%,while the NIRS technology diagnostic accuracy was in the range of 50μ*** the first time,this novel technique provides physicians with real-time burn depth information objectively and accurately.

关键词： Burn depth Near infrared spectroscopy Support vector regression

Neoadjuvant radiotherapy dose escalation for locally advanced rectal cancers in the new era of radiotherapy:A review of literature

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World Journal of Clinical Cases 2021年第30期9卷 9077-9089页

作者： Durim Delishaj ilaria Costanza Fumagalli Stefano Ursino Agostino Cristaudo Francesco Colangelo Antonio Stefanelli Alessandro Alghisi Giuseppe De Nobili Romerai D’Amico Alessandra Cocchi Antonio Ardizzoia Carlo Pietro Soatti Department of Radiation Oncology Alessandro Manzoni HospitalLecco 23900Italy Department of Radiation Oncology San Donato HospitalMilan 20097Italy Department of Radiation Oncology Santa Chiara University HospitalPisa 56126Italy Royal preston Hospital Lancashire Teaching Hospital-NHS TustPreston PR29HTUnited Kingdom Department of Radiation Oncology Azienda Ospedaliero-Universitaria di FerraraFerrara 44124Italy Department of Clinical Oncology Alessandro Manzoni HospitalLecco 23900Italy

BACKGROUND The standard treatment of locally advanced rectal cancers(LARC)consists on neoadjuvant chemoradiotherapy followed by total mesorectal *** data in literature showed a benefit on tumor downstaging and pathological complete response(pCR)rate using radiotherapy dose escalation,however there is shortage of studies regarding dose escalation using the innovative techniques for LARC(T3-4 or N1-2).AIM To analyze the role of neoadjuvant radiotherapy dose escalation for LARC using innovative radiotherapy *** In December 2020,we conducted a comprehensive literature search of the following electronic databases:PubMed,Web of Science,Scopus and Cochrane *** limit period of research included articles published from January 2009 to December *** by title and abstract was carried out to identify only studies using radiation doses equivalent dose 2 Gy fraction(EQD2)≥54 Gy and Volumetric Modulated Arc Therapy(VMAT),intensity-modulated radiotherapy or image-guided radiotherapy(IGRT)*** authors’searches generated a total of 2287 results and,according to prISMA Group(2009)screening process,21 publications fulfil selection criteria and were included for the *** The main radiotherapy technique used consisted in VMAT and IGRT *** mainly dose prescription was 55 Gy to high risk volume and 45 Gy as prophylactic volume in 25 fractions given with simultaneous integrated boosts technique(42.85%).The mean pCR was 28.2%with no correlation between dose prescribed and response rates(P value≥0.5).The R0 margins and sphincter preservation rates were 98.88%and 76.03%,*** a mean follow-up of 35 months local control was 92.29%.G3 or higher toxicity was 11.06%with no correlation between dose prescription and *** receiving EQD2 dose>58.9 Gy and BED>70.7 Gy had higher surgical complications rates compared to other group(P value=0.047).CONCLUSION Dose escalation neoadjuvant radiotherapy usi

关键词： Rectal cancer Radiotherapy Volumetric Modulated Arc Therapy Imageguided radiotherapy Intensity-modulated radiotherapy Neoadjuvant radiotherapy