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Detection of Chlamydophila psittaci from feral pigeons in environmental samples:problems with currently available techniques

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Integrative Zoology 2010年第1期5卷 63-69页

作者： ila geigenfeind Daniel HA AG-WACK ERNAGEL Department of Biomedicine Institute of AnatomyUniversity of BaselBaselSw itzerland

Chlamydophila psittaci(Lillie,1930)Everett et al.,1999,the pathogenic agent of human ornithosis,is widespreadin feral pigeon populations and many cases of transmission from feral pigeons to humans have been *** of the present study was to detect *** in environmental samples to find out more about possibletransmission routes and,therefore,to assess the zoonotic risk for *** samples were collected from nestboxes in a feral pigeon ***,samples were taken from the feather dust film covering the water surface ofpublic fountains where pigeons regularly *** samples were tested for the presence of chlamydial antigenusing an antigen enzyme-linked immunosorbent assay to prove shedding of *** by feral *** testdetects a genus specific lipopolysaccharide in the outer membrane of the chlamydial *** were testedusing the IDEIA PCE Chlamydia Test kit(DakoCytomation)and positive results were verified with IDEIA ChlamydiaBlocking Reagents(DakoCytomation).The IDEIA PCE Chlamydia Test yields a high proportion of positive ***,when IDEIA Chlamydia Blocking was performed,most of the positive results turned out to be negative orcould not be *** conclude that antigen-enzyme-linked immunosorbent assay tests are not suitable fordetecting *** in environmental *** publications where no blocking test was used should bereconsidered critically.

关键词： antigen-enzyme-linked immunosorbent assay Chlamydophila psittaci Columba livia environmen-tal samples zoonosis

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