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Long-term culture of primary porcine mature hepatocytes in the medium supplemented with ascorbic acid 2-phosphate

Natural Science 2010年第11期2卷 1264-1273页

作者： Yohichi kumaki iku kumaki Xiaomei Guo Weilin Shang Toshie Koyama Ai Okamura Yoshiaki Shiba Toshiyuki Mukaiyama Noriko Sasaki Makoto Kodama 不详

In this study, the effect of ascorbic acid 2-phosphate (Asc2P) was tested on porcine and rat mature hepatocytes in vitro. a). Asc2P increased the porcine, but not rat, albumin secretion and mRNA expression. The enhancing effect of Asc2P on porcine C/EBP alpha mRNA was observed in porcine mature hepatocytes. These data suggested that Asc2P played an important role in the regulation of porcine albumin mRNA level. b). The enhancing effect of Asc2P on ammonium metabolic activity was also observed in porcine, but not rat, mature hepatocytes. The porcine ornithine transcarbamylase (OTC) and arginase mRNAs were augmented by Asc2P, indicating that Asc2P had a direct effect on the urea cycle. c). The porcine collagen type I and type III mRNA, but not type XII mRNA, were detected as well, sugessting that Asc2P did not have the effect on the non-parenchymal hepatocytes to induce collagen type I and III mRNA expression. d). Our RT-PCR analysis demonstrated that the porcine hepatocytes expressed the sodium-ascorbate co-transporters SVCT1 and SVCT2, however, the intensities of porcine sodium-ascorbate co-transporters SVCT1 and SVCT2 bands were not changed markedly. These findings indicated that the Asc2P had no effect on SVCT1 and SVCT2 mRNA expression. e). The enhancing effect of Asc2P on porcine albumin mRNA was inhibited by staurosporine, a portein kinase inhibitor. We conclude that the enhanced albumin mRNA by Asc2P might be due to activation of tyrosine protein kinase and/or PKC and the Asc2P enhanced porcine albumin mRNA mainly at the transcriptional step.

关键词： Albumin Secretion Ammonium Metabolic Activity Ascorbic Acid 2-Phosphate Porcine Hepatocytes Reverse Transcriptase-Polymerase Chain Reaction

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SYNERGISTIC EXTRACTION OF EUROPIUM（Ⅲ） WITH 2-THENOYLTRIFLUOROACETONE AND A LEWIS BASE AND THE APPLICATION TO PREPARATION OF BINUCLEAR COMPLEXES

SYNERGISTIC EXTRACTION OF EUROPIUM（Ⅲ） WITH 2-THENOYLTRIFL...

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International Solvent Extraction Conference 2005

作者： Yuko Hasegawa Satoshi kumaki Koichiro Tanaka Department of Chemistry Faculty of Science Science University of Tokyo Kagurazaka Shinjuku-ku Tokyo 162-8601 Japan Department of Chemistry Faculty of Science Science University of Tokyo Kagurazaka Shinjuku-ku Tokyo 162-8601 Japan Department of Chemistry Faculty of Science Science University of Tokyo Kagurazaka Shinjuku-ku Tokyo 162-8601 Japan

In the extraction of lanthanoids(III) with 2-thenoyltrifluoroacetone (Htta) and a Schiff base complexed copper(II), the distribution ratio of europium(III) has been measured as a function of pH, Htta

关键词： Synergistic extraction Schiff base Cu(saltn) lanthanoids(III) stability constants binuclear complexes

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Effect of double platinum agents, combination of miriplatintransarterial oily chemoembolization and cisplatinhepatic arterial infusion chemotherapy, in patients with hepatocellular carcinoma: Report of two cases

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World Journal of Clinical Cases 2017年第6期5卷 238-246页

作者： Kohei Ogawa Kenya Kamimura Yukari Watanabe Yosuke Motai Daisuke kumaki Ryoya Seki Akira Sakamaki Satoshi Abe Hirokazu Kawai Takeshi Suda Satoshi Yamagiwa Shuji Terai Division of Gastroenterology and Hepatology Graduate School of Medical and Dental Sciences Niigata University Department of Gastroenterology and Hepatology Uonuma Institute of Community Medicine Niigata Medical and Dental Hospital

Hepatocellular carcinoma(HCC) is one of the most common cancers and the third highest cause of cancerassociated mortality worldwide. The treatment of HCC is complicated by its variable biological behavior and the frequent coexistence of chronic liver disease, particularly cirrhosis. To date, multiple treatment modalities have been developed according to the stage of the tumor and the hepatic functional reserve, including transarterial treatments such as transarterial chemoembolization, transarterial oily chemoembolization(TOCE), and hepatic arterial infusion chemotherapy(HAIC). We conducted a phase I and II study of the combination therapy with double platinum agents, miriplatin and cisplatin, and confirmed its safety and efficacy. Here, we describe two cases of unresectable HCC who were successfully treated by miriplatin-TOCE/cisplatin-HAIC combination therapy, resulting in complete responses with no significant adverse events. This report will provide that the combination therapy can be the therapeutic option for HCC patients in the advanced stage.

关键词： Hepatocellular carcinoma Double platinum Transarterial oily chemoembolization Hepatic arterial infusion chemotherapy Combination

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具有广泛回弹性和硬度的泡沫的聚醚

具有广泛回弹性和硬度的泡沫的聚醚

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中国聚氨酯工业协会第十次年会

作者： Masakazu Kageoka Masaki Kuwagaki Hideaki Ootsuka Yasuhiko Sato Takashi kumaki 武田药品工业株式会社

介绍如何选择最佳聚醚而生产最理想的泡沫。特别注意分析其与泡沫物性(硬度和回弹性)的关系。同时介绍一些聚醚(ACTCOL),包括新开发的聚醚。

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Fatal liver failure due to reactivation of lamivudine-resistant HBV mutant

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World Journal of Gastroenterology 2004年第11期10卷 1686-1687页

作者： Tatehiro Kagawa Norihito Watanabe Hisashi Kanouda Ichiro Takayama Tadahiko Shiba Takashi Kanai Kazuya Kawazoe Shinji Takashimizu Nobue kumaki Kazuo Shimamura Shohei Matsuzaki Tetsuya Mine Department of Internal Medicine Tokai University School of MedicineBoseidaiIseharaKanagawa 259-1193Japan Department of Pathology Tokai University School of MedicineBoseidaiIseharaKanagawa 259-1193Japan Department of Internal Medicine Tokai University School of MedicineBoseidai IseharaKanagawa 259-1193Japan

We report a case of fatal liver failure due to reactivation of lamivudine-resistant HBV. A 53-year-old man was followed since 1998 for HBV-related chronic hepatitis. Serum HBV-DNA was 150 MEq/mL (branched DNA signal amplification assay) and ALT levels fluctuated between 50-200 IU/L with no clinical signs of liver cirrhosis. Lamivudine (100 mg/d) was started in May 2001 and serum HBV-DNA subsequently decreased below undetectable levels. In May 2002, serum HBV-DNA had increased to 410 MEq/mL, along with ALT flare (226 IU/L). The YMDD motif in the DNA polymerase gene had been replaced by YIDD. Lamivudine was continued and ALT spontaneously decreased to the former levels. On Oct 3 the patient presenting with general fatigue, nausea and jaundice was admitted to our hospital. The laboratory data revealed HBV reactivation and liver failure (ALT: 1828 IU/L, total bilirubin: 10 mg/dL, and prothrombin INR: 3.24). For religious reasons, the patient and his family refused blood transfusion, plasma exchange and liver transplantation. The patient died 10 d after admission. The autopsy revealed remarkable liver atrophy.

关键词：肝硬变肝细胞再生免疫能力 HBV 基因突变肝炎

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