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Distribution and relative activity of matrix metalloproteinase-2 in human coronal dentin

International Journal of Oral Science 2011年第4期3卷 192-199页

作者： Lee W. Boushell Masaru kaku Yoshiyuki Mochida Mitsuo Yamauchi Department of Operative Dentistry School of Dentistry University of North Carolina Department of Bio-Prosthodontics Medical and Dental Science Niigata University Graduate School Chuo-ku Department of Periodontology and Oral Biology Division of Oral Biology Boston University Henry M. Goldman School of Dental Medicine Center for Advanced Biomedical Research Dental Research Center School of Dentistry University of North Carolina

The presence of matrix metalloproteinase-2 （MMP-2） in dentin has been reported, but its distribution and activity level in mature human coronal dentin are not well understood. The purpose of this study was to determine the MMP-2 distribution and relative activity in demineralized dentin. Crowns of twenty eight human molars were sectioned into inner （ID）, middle （MD）, and outer dentin （OD） regions and demineralized. MMP-2 was extracted with 0.33 mol·L-1 EDTA/2 mol·L-1 guanidine-HCl, pH 7.4, and MMP-2 concentration was estimated with enzyme-linked immunoabsorbant assay （ELISA）. Further characterization was accomplished by Western blotting analysis and gelatin zymography. The mean concentrations of MMP-2 per mg dentin protein in the dentin regions were significantly different （P=0.043）： 0.9 ng （ID）, 0.4 ng （MD）, and 2.2 ng （OD）, respectively. The pattern of MMP-2 concentration was OD〉ID〉MD. Western blotting analysis detected -66 and -72 kDa immunopositive proteins corresponding to pro- and mature MMP-2, respectively, in the ID and MD, and a -66 kDa protein in the OD. Gelatinolytic activity consistent with MMP-2 was detected in all regions. Interestingly, the pattern of levels of Western blot immunodetection and gelatinolytic activity was MD〉ID〉OD. The eoneentration of MMP-2 in human coronal dentin was highest in the region of dentin that contains the dentinoenamel junction and least in the middle region of dentin. However, levels of Western blot immunodetection and gelatinolytic activity did not correlate with the estimated regional concentrations of MMP-2, potentially indicating region specific protein interactions.

关键词： matrix metalloproteinase-2 human coronal dentin distribution gelatinolytic activity

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Five Stylized Facts on Belt and Road Countries and Their Trade Patterns

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China & World Economy 2023年第1期31卷 149-181页

作者： kaku Attah Damoah Giorgia Giovannetti Enrico Marvasi Department of Economics and Management University of FlorenceItaly Department of Economics and Management University of FlorenceItalyand European University InstituteItaly Department of Economics Roma Tre UniversityItaly

The Belt and Road Initiative(BRI)offers investment opportunities for several Eurasian countries but not all of them attract investments in the same *** paper investigates the geographical distribution of BRI projects completed between 2013 and *** analysis shows that pre-existing trade patterns are related to the likelihood of a country receiving completed BRI *** single out and provide evidence in support of five stylized ***,BRI countries with completed projects tend to be poorer and ***,projects are more likely to occur in countries with intense intermediate trade with ***,the countries that received projects have more diversified export structures and their sectoral specialization overlaps with that of ***,among middle-high-income countries,the allocation ofprojects tends to favor those with high levels of intra-industry ***,among BRI countries with projects,the complexity or sophistication of the goods traded increases faster with *** findings suggest that fostering trade integration has direct benefits and may also contribute to further BRI investments.

关键词： Belt and Road Initiative China global value chains network trade in intermediates

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Chemoradiotherapy with twice-weekly administration of low-dose gemcitabine for locally advanced pancreatic cancer

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World Journal of Gastroenterology 2008年第34期14卷 5311-5315页

作者： Hisato Igarashi Tetsuhide Ito Ken Kawabe Terumasa Hisano Yoshiyuki Arita Toyoma kaku Ryoichi Takayanagi Department of Medicine and Bioregulatory Science Graduate School of Medical ScienceKyushu University

AIM:To evaluate the chemoradiotherapy for locally advanced pancreatic cancer utilizing low dose gemcitabine as a radiation sensitizer administered twice weekly. METHODS: We performed a retrospective analysis of chemoradiotherapy utilizing gemcitabine administered twice weekly at a dose of 40 mg/m2. After that, maintenance systemic chemotherapy with gemcitabine, at a dose of 1000 mg/m2, was administered weekly for 3 wk with 1-wk rest until disease progression or unacceptable toxicity developed. RESULTS: Eighteen patients with locally advanced unresectable pancreatic cancer were enrolled. Three of those patients could not continue with the therapy; one patient had interstitial pneumonia during radiation therapy and two other patients showed liver metastasis or peritoneal metastasis during an early stage of the therapy. The median survival was 15.0 mo and the overall 1-year survival rate was 60%, while the median progression-free survival was 8.0 mo. The subgroup which showed the reduction of tumor development, more than 50% showed a tendency for a better prognosis; however, other parameters including age, gender and performance status did not correlate with survival. The median survival of the groups that died of liver metastasis and peritoneal metastasis were 13.0 mo and 27.7 mo, ***: Chemoradiotherapy with low-dose gemcitabine administered twice weekly could be effective to patients with locally advanced pancreatic cancer; however, patients developing liver metastases had a worse prognosis. Another chemoradiotherapy strategy might be needed for those patients, such as administrating one or two cycles of chemotherapy initially, followed by chemoradiotherapy for the cases with no distant metastases.

关键词： Advanced pancreatic cancer Chemoradio-therapy Gemcitabine Radiosensitizer Tumor marker

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Generalized periodontitis treated with periodontal,orthodontic,and prosthodontic therapy:A case report

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World Journal of Clinical Cases 2021年第21期9卷 6110-6124页

作者： Masato kaku Shinji Matsuda Takayasu Kubo Saiji Shimoe Kazuhiro Tsuga Hidemi Kurihara Kotaro Tanimoto Department of Anatomy and Functional Restorations Hiroshima University Graduate School of Biomedical SciencesHiroshima 734-8553Japan Department of Periodontal Medicine Hiroshima University Graduate School of Biomedical SciencesHiroshima 734-8553Japan Department of Advanced Prosthodontics Hiroshima University Graduate School of Biomedical SciencesHiroshima 734-8553Japan Department of Orthodontics and Craniofacial Developmental Biology Hiroshima University Graduate School of Biomedical SciencesHiroshima 734-8553Japan

BACKGROUND Generalized periodontitis is a severe periodontal disease characterized by rapid periodontal destruction in healthy *** case report describes the treatment of a severe crowding,large overjet,and occlusal collapse due to the loss of anterior guidance with generalized *** SUMMARY A 35-year-old female patient with a chief complaint of crowding and maxillary protrusion was diagnosed with generalized periodontitis by clinical and radiographic *** improve crowding and overjet,orthodontic treatment was performed after basic periodontal *** damaged upper right lateral incisor and left canine were extracted,and lower right first premolar and left second premolar were also removed to treat severe *** orthodontic treatment,periodontal flap surgery for upper left molars and guided tissue regeneration for the lower left second molar was ***,a dental implant was inserted in the upper left canine *** esthetics of the maxillary anterior tooth was improved by prosthetic *** treatment result showed a well-improved occlusion with proper anterior guidance and healthy periodontal tissue after a retention period of 10 *** Periodontal,orthodontic,and prosthodontic treatments are extremely useful to improve function and stable periodontal tissue for generalized periodontitis.

关键词： Generalized periodontitis Orthodontic treatment Periodontal regenerative therapy Prosthodontic treatment Comprehensive dental treatment Long-term case study Case report

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Fractalkine and TGF-β1 levels reflect the severity of chronic pancreatitis in humans

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World Journal of Gastroenterology 2008年第42期14卷 6488-6495页

作者： Mikihiko Yasuda Tetsuhide Ito Takamasa Oono Ken Kawabe Toyoma kaku Hisato Igarashi Taichi Nakamura Ryoichi Takayanagi Department of Medicine and Bioregulatory Science Graduate School of Medical Sciences Kyushu University Fukuoka 812-8582 Japan

MM： To clarify whether serum chemokine and cytokine levels can become useful biological and functional markers to assess the severity of chronic pancreatitis （CP）. This study aimed at clarifying whether serum chemokine and cytokine levels can become useful biological and functional markers to assess the severity of CR METHODS： Serum monocyte chemoattractant protein-1 （MCP-1）, transforming growth factor beta-1 （TGF-βI）, and soluble type fractalkine （s-fractalkine） concentrations were examined in patients with CP （n = 109） and healthy controls （n = 116）. Severity of disease was classified in patients with CP by a staging system. Relationships between stage-specific various clinical factors and serum MCP-1, TGF-β1, and s-fractalkine levels were investigated. Furthermore, 57 patients with non-alcoholic CP were similarly evaluated in order to exclude influence of alcohol intake. RESULTS： Patients with CP showed significant higher levels of serum TGF-β1 and s-fractalkine, but not MCP-1, compared to the controls. Serum TGF-β1 in the severe stage and s-fractalkine in the mild and thesevere stage of CP significantly increased compared to those of controls. However, it was observed that both TGF-β1 and s-fractalkine levels were affected by alcohol intake. In patients with non-alcoholic CP, serum TGF-β1 showed significant increase in the moderate stage of CP, and serum s-fractalkine revealed significant increase in the early stage of CP. CONCLUSION： It is suggested that the measurement of serum F-fractalkine is useful to diagnose early- stage CP. Moreover, the combined determination of both, s-fractalkine and TGF-β1, in human sera may be helpful in evaluating the severity status of CP.

关键词： Chronic pancreatitis Transforming growth factor beta-l Soluble fractalkine Monocyte chemoattractant protein-1

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基于液滴定向反弹的混合润湿性表面设计准则

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Transactions of Nanjing University of Aeronautics and Astronautics 2023年第2期40卷 159-168页

作者：张童伟 kaku Chuyo 李美萱吴杰江苏大学汽车与交通工程学院镇江212013 江苏超力电器有限公司技术研究院镇江212321中国南京航空航天大学航空学院南京210016 西北工业大学翼型、叶栅空气动力学国家级重点实验室西安710072

液滴撞击固体表面的定向反弹在防结冰/起雾、自清洁等工程应用中具有重要意义。混合润湿性表面已被证明是一种有效的液滴操纵方法。本文对液滴撞击构筑有亲水条纹的疏水基底进行数值模拟研究,采用已验证的扩散界面法来捕获界面演化。首... 详细信息

液滴撞击固体表面的定向反弹在防结冰/起雾、自清洁等工程应用中具有重要意义。混合润湿性表面已被证明是一种有效的液滴操纵方法。本文对液滴撞击构筑有亲水条纹的疏水基底进行数值模拟研究,采用已验证的扩散界面法来捕获界面演化。首先,研究卫星液滴在撞击过程中的形成过程,通过分析液滴的垂直速度和横向速度,明确混合润湿性表面在液滴扩散、收缩和反弹阶段中的作用。然后,系统探究条纹宽度对液滴反弹形式和接触时间的影响,重点关注液膜演化和液滴弹跳过程中的动力学和能量传递机制。所得结果可以指导混合润湿性表面的优化设计和液滴定向回弹的控制。

关键词：液滴撞击混合润湿性表面定向反弹设计准则

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Synthesis of a Rhodamine-Appended Cyclophane as a Fluorescence Host in Water

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Advances in Chemical Engineering and Science 2014年第4期4卷 401-408页

作者： Osamu Hayashida Yu kaku Department of Chemistry Faculty of Science Fukuoka University Fukuoka Japan

A cationic water-soluble cyclophane (1a) having a rhodamine moiety as a red-fluorescence fluorophore was prepared by reaction of a monoamine derivative of tetraaza[6.***.6.1]paracyclophane having three N-t-butoxycarbonyl-β-alanine residues with rhodamine B isothiocyanate, followed by removal of the protecting groups. The guest-binding behavior of 1a toward anionic guests such as dabsyl derivative and 4-(1-pyrene)butanoate was investigated by fluorescence spectroscopy. The results suggested the formation of host-guest complexes with a stoichiometric ratio of 1:1 and the binding constants (K) of the host-guest complexes were evaluated.

关键词： Host-Guest Chemistry Water-Soluble Cyclophane Fluorescent Sensing

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Loosening Xyloglucan Accelerates the Enzymatic Degradation of Cellulose in Wood

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Molecular Plant 2009年第5期2卷 904-909页

作者： Rumi Kaida Tomomi kaku Kei'ichi Baba Masafumi Oyadomari Takashi Watanabe Koji Nishida Toshiji Kanaya Ziv Shani Oded Shoseyov Takahisa Hayashi Kyoto University RISH Gokasho Uji Kyoto 611-0011 Japan Kyoto University Institute for Chemical Research Gokasho Uji Kyoto 611-0011 Japan CBD Technologies Rehovot 76100 Israel Institute of Sustainability Science Kyoto University Gokasho Uji Kyoto 611-0011 Japan

In order to create trees in which cellulose, the most abundant component in biomass, can be enzymatically hydrolyzed highly for the production of bioethanol, we examined the saccharification of xylem from several transgenic poplars, each overexpressing either xyloglucanase, cellulase, xylanase, or galactanase. The level of cellulose degradation achieved by a cellulase preparation was markedly greater in the xylem overexpressing xyloglucanase and much greater in the xylems overexpressing xylanase and cellulase than in the xylem of the wild-type plant. Although a high degree of degradation occurred in all xylems at all loci, the crystalline region of the cellulose microfibrUs was highly degraded in the xylem overexpressing xyloglucanase. Since the complex between microfibrils and xyloglucans could be one region that is particularly resistant to cellulose degradation, loosening xyloglucan could facilitate the enzymatic hydrolysis of cellulose in wood.

关键词： Overexpression of xyloglucanase saccharification transgenic poplar xylem.

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Quantitative Analysis of Nursing Observation Employing a Portable Eye-Tracker

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Open Journal of Nursing 2016年第1期6卷 53-61页

作者： Norie Suetsugu Masafumi Ohki Tsunehisa kaku Department of Nursing Faculty of Medicine Saga University Saga Japan Department of Health Sciences Graduate School of Medical Sciences Kyushu University Fukuoka Japan

Background: Observation is an important skill for making appropriate nursing decisions and engaging in good practice. However, experts’ observation behavior and cognitive processes cannot be easily verbalized or documented in an objective and accurate manner. Quantitative analysis of the observation behavior of nurses with rich clinical experience will yield effective educational data for fostering and improving nursing students’ observation skills. Objectives: To improve nursing assessment education, the differences in the information gathering processes between clinical nurses and nursing students were analyzed by using a portable eye-tracker. Design: An experimental study. Settings: The experiment was performed at a university in Japan. Participants: The participants were 11 clinical nurses with at least 5 years of clinical experience for postoperative patients, and 10 fourth-year nursing students. Methods: In a mock hospital room, wherein we recreated a situation where a patient in postoperative day 1 was confined to a bed, participants wore an eye-tracking camera and engaged in nursing observation to make an early postoperative ambulation assessment of the patient. Participants’ gaze points and gaze fixation durations were extracted from the gaze measurement data and compared. Results: Clinical nurses had shorter observation times and gaze durations than did nursing students, and focused more on the patient chart, intravenous drip, and indwelling drain. Students gazed for longest at the measuring devices for vital signs. Conclusions: We quantitatively analyzed differences in nursing observation according to clinical experience. Although no significant difference was found in gaze points, nursing students had a greater tendency to focus on information that was numerically displayed. Nurses with clinical experience conducted observations by gazing at information that they needed to focus on the most according to the patients’ postoperative course.

关键词： Nursing Observation Eye-Tracker Postoperative Ambulation Assessment Quantitative Analysis

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采用神经网络与模糊控制的制动需求识别

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中国机械工程 2020年第23期31卷 2847-2855页

作者：刘晏宇喻凡宋娟娟庞纪苏 kaku Chuyo 郭中陽上海交通大学机械与动力工程学院上海200240 浙江力邦合信智能制动系统股份有限公司上海分公司上海201702

以保证车辆的制动安全性为最终目的,基于制动推杆行程传感器信号,利用神经网络算法与模糊控制算法提出了对制动需求进行识别的方法。首先采用神经网络算法与模糊控制算法完成对驾驶员制动需求的初步判断,然后通过主缸压力反馈信号对制... 详细信息

以保证车辆的制动安全性为最终目的,基于制动推杆行程传感器信号,利用神经网络算法与模糊控制算法提出了对制动需求进行识别的方法。首先采用神经网络算法与模糊控制算法完成对驾驶员制动需求的初步判断,然后通过主缸压力反馈信号对制动系统的制动动作完成程度进行预测,进而对制动需求识别结果进行持续反馈跟踪修正,以保证车辆的制动系统能够实现预期的制动效能。通过MATLAB/Simulink仿真试验与台架试验对控制算法进行了对比与验证,结果表明该方法能迅速准确地识别驾驶员的制动需求,从而为车辆的制动安全性提供保障。

关键词：安全制动神经网络模糊控制制动力

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