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Morphological substantiation of application of cellular technologies for correction of striae

Health 2010年第12期2卷 1430-1436页

作者： igor maiborodin Andrey Shevela Andrey Babko Vitaly Morozov Vera Matveeva 不详

The possibility of the application of autologic pluripotent cells from peripheral blood (APCPB) for correction of striae after pregnancy was studied. Visually first signs of improvement of atrophic scars can be noticed in 6-8 weeks after injection of APCPB and gradually progress during 6 months. The atrophy of epithelium, hyperkeratosis, sclerosis of all dermis layers with smoothing of papillae, reduction of number of blood vessels and skin appendages were found before treatment by method of light mi-croscopy. In all observations after injection of APCPB in a connective tissue, which replaces of dermis, an increase of number of vessels at the expense of processes of neoangiogenesis, leukocytic infiltration reduction, thinning and ordering of arrangement of collagen and elastin fibers were detected. These results give a chance for more successful application of cosmetology procedures for correction of these defects of skin.

关键词： Skin Striae after Pregnancy Atrophy of Epidermis Dermal Sclerosis Pluripotent Cells from Peripheral Blood Neoangiogenesis

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Angiogenesis in Rat Uterine Scar after Introduction af Autological Mesenchymal Stem Cells of Bone Marrow Origin

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Journal of Biomedical Science and Engineering 2011年第3期4卷 164-172页

作者： igor maiborodin Natalia Yakimova Vera Matveeva Andrey Shevela Elena maiborodina Ekaterina Pekareva Olesya Tkachuk 不详

The results of injecting of autologic mesenchymal stem cells of bone marrow origin (AMSCBMO), transfected by the GFP gene, into the scar of rat uterine horns were studied by methods of light microscopy. After the introduction of AMSCBMO into the formed scar on the right (2 months after the ligation) large groups of blood vessels with cellular elements inside were present;groups like that were not found in the opposite side. Studying unstained sections under reflected ultraviolet light the sufficient bright luminescence in the endothelium and the external membrane of scar vessels was found in uterine horn only on the side of introduction of AMSCBMO. It was concluded that after the introduction of AMSCBMO into the scar tissue they form blood vessels by differentiation into endotheliocytes and pericytes. GFP gene expression not only in endothelium of vessels, but also in their external membrane indicates that differentiation of AMSCMBO is possible in endothelial and in pericytal directions.

关键词： uterine scar mesenchymal stem cells angiogenesis endotheliocytes pericytes

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Experimental Results of the Fibrin Clot Use to Accelerate the Regeneration of Damaged Bone in the Rat Lower Jaw

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Surgical Science 2010年第1期1卷 1-6页

作者： igor maiborodin Andrey Shevela Tatyana Perrin Ivan Kolesnikov Vera Matveeva Alexander Shevela Boris Sheplev Irina Kolmakova 不详

Morphological and radiological methods were used to study regeneration of the damaged bone of rat mandibles after application of plateletenriched fibrin clot. A bone hole was artificially created, and in the natural course of regeneration, the hole was immediately filled with blood and there a blood clot formed. After one week of healing, separate islands of young bone tissue appeared. After two to three weeks, the opening in the mandible was completely replaced by the young bone tissue. When a similar bone hole was filled with autological fibrin clot, the blood clot did not form. But after one week the entire hole was filed with newlyformed fused bone tissue. By the second week after the use of fibrin clot, the bone hole had further healed and bone callus was formed.

关键词： Fibrin Clot Regeneration of Bone Density of Bone Tissue

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