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Cytotoxic (A549) and antimicrobial effects of Methylobacterium sp.isolate (ERI-135) from Nilgiris forest soil,India

Asian Pacific Journal of Tropical Biomedicine 2012年第9期2卷 712-716页

作者： Balachandran C Duraipandiyan V ignacimuthu s Division of Microbiology Entomology Research InstituteLoyola College Department of Botany and Microbiology Addiriyah Chair for Environmental StudiesCollege of ScienceKing Saud University

Objective:To assess the antimicrobial and cytotoxic effects of Methylobacterium sp.isolated from soil sample of Doddabetta forest,Nilgiris,Western Ghats of Tamil Nadu.Methods:Isolation of Methylobacterium was performed from soils by serial dilution plate technique.The strain was grown in modified nutrient gulucose agar(MNGA)medium to study the morphology and biochemical characteristics.Methylobacterium sp.was screened for its antimicrobial activity against pathogenic bacteria and fungi.The strain was subjected to 16s rRNA analysis and was identified as Methylohacterium sp.The nucleotide sequence of the 16s rRNA gene of the isolate exhibited close similarity with other Methylobacterium sp.and has been submitted to Genbank.The antibacterial substances were extracted using chloroform and ethyl acetate from MNGA medium in which ERI-135 had grown for 5 d at 30℃.Cytotoxic effect was also studied.GC-Ms analysis was carried out.The antimicrobial activity was assessed using broth micro dilution technique.Results:Ethyl acetate extract showed activity against bacteria such as Bacillus subtilis,Klebsiella pneumoniae(K.pneumoniae),Pseudomonas aeruginosa,salmonella typkimurium,shigella flexneri,Enterobacter aerogenes,staphylococcus aureu and staphylococcus epidermidis(s.epidermidis)and fungi such as,Candida albicans and Trichophyton rubrum.The lowest minimum inhibitory concentrations were:250μg/mL against 5.epidermidis and 250μg/mL against K.pneumonia.The isolate had the ability to produce enzymes such as protease.The exyract showed cytotoxic effect in human adenocarcinoma cancer cell line(A549).GC-Ms analysis showed the presence of isovaleric acid(3.64%),2-Methylbulanoic acid(5.03%),isobutyramide(5.05%),N,Noimethylformamide-di-t-butylacetal(9.79%),benzeneacetamide(15.56%),octyl butyl phthalate(3.59%)and diisooctyl phthalate(5.79)in the extract.Conclusions:Methylobaeterium sp.(ERI-135)showed promising antibacterial and cytotoxic activity.This is the first report in the antimicrobial and cytotoxic effect of Methylobaeterium sp.

关键词： Methylobacterium sp l6s rRNA Antimicrobial Cytotoxicity GC-Ms soil sample Western Ghats

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Characterization and phylogenetic analysis of novel polyene type antimicrobial metabolite producing actinomycetes from marine sediments:Bay of Bengal India

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Asian Pacific Journal of Tropical Biomedicine 2012年第10期2卷 803-810页

作者： Valan Arasu M Asha KRT Duraipandiyan V ignacimuthu s Agastian P Division of Microbiology Entomology Research InstituteLoyola College Department of Biochemistry Malankara Catholic CollegeMariyagiriKanyakumari District Department of Botany and Microbiology Addiriyah Chair for Environmental StudiesCollege of ScienceKing Saud University Department of Plant Biology and Biotechnology Loyola College

Objective:To isolate and indentify the promising antimicrobial metabolite producing streptomyces strains from marine sediment samples from Andraprudesh coast of India.Methods:Antagonistic aetinomycetes were isolated by starch casein agar medium and modified nutrient agar medium with 1%glucose used as a base for primary screening.significant antimicrobial metabolite producing strains were selected and identified by using biochemical and 16s rDNA level.Minimum inhibitory concentrations of the organic extracts were done by using broth micro dilution method.Results:Among the 210 actinomyeetes,64.3%exhibited activity against Gram positive bacteria,48.5%showed activity towards Cram negative bacteria,38.8%exhibited both Cram positive and negative bacteria and 80.85%isolates revealed significant antifungal activity.However,five isolates AP-5,AP-18,AP-41 and AP-70 showed significant antimicrobial activity.The analysis of cell wall hydrolysates showed the presence of LL-diaminopimelic acid and glycine in all the isolates.sequencing analysis indicated that the isolates shared 98.5%-99.8%sequence identity to the 16s rDNA gene sequences of the streptomyces taxons.The antimicrobial substances were extracted using hexane and ethyl acetate from spent medium in which strains were cultivated at 30X for five days.The antimicrobial activity was assessed using broth micro dilution technique.Each of the culture extracts from these five strains showed a typical polyenelike property.The lowest minimum inhibitory concentrations of ethyl acetate extracts against Escherichia coli and Cumularia lunula were 67.5 and 125.0μg/mL,respectively.Conclusions:It can be concluded that hexane and ethyl acetate soluble extracellular products of novel isolates are effective against pathogenic bacteria and fungi.

关键词： Marine streptomyces sp. 16s rDNA Polyene antimicrobial activity Minimum inhibitory concentration

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Larvicidal and knockdown effects of some essential oils against Culex quinquefasciatussay, Aedes aegypti(L.) and Anopheles stephensi(Liston)

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Advances in Bioscience and Biotechnology 2012年第7期3卷 855-862页

作者： A. Manimaran M. Mary Jee Jee Cruz C. Muthu s. Vincent s. ignacimuthu Department of Advanced Zoology and Biotechnology Loyola College Chennai India Department of Biological sciences AVIT Vinayaka Missions University Payyanoor Campus Salem India Entomology Research Institute Loyola College Chennai India

Efficacy of 25 essential oils was screened against filarial vector, Culex quinquefasciatus, for their larvicidal and knockdown effects in a preliminary study. Of these, 8 oils viz. calamus oil, cinnamon oil, citronella oil, clove oil, eucalyptus oil, lemon oil, mentha oil and orange oil exhibited 100% larvicidal activity at 1000 ppm and 100% knockdown effect at 10% concentration. These 8 oils were screened further against Cx. Quinquefasciatus, Aedes aegypti and Anopheles stephensi for their larvicial and knockdown effects at different concentrations. Mentha oil was the most promising against An. stephensi and Ae. Aegypti recording LC50 and LC90 values of 39.74 and 115.67 ppm and 46.23 and 165.36 ppm, respectively for larvicidal activity. Calamus oil was the most effective against Cx. Quinquefasciatus with LC50 and LC90 values of 40.40, and 140.07 ppm, respectively for larvicidal activity. Orange oil showed the most potent knockdown effect with the KT50 and KT95 values of 27.44, 26.22 and 29.91 and 70.81, 65.33 and 68.57 min, against An. stephensi, Cx. Quinquefasciatus and Ae. Aegypti, respectively. The results clearly indicated that mentha oil and calamus oil were the most promising larvicides and orange oil had potent knockdown effect against the tested mosquito species. These oils could be used to develop a new formulation to control mosquitoes.

关键词： Larvicidal Knockdown Essential Oils Aedes aegypti Anopheles stephensi Culex quinquefasciatus

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Antibacterial activity of some actinomycetes from Tamil Nadu,India

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Asian Pacific Journal of Tropical Biomedicine 2012年第12期2卷 936-943页

作者： Pachaiyappan saravana Kumar John Poonga Preetam Raj Veeramuthu Duraipandiyan savarimuthu ignacimuthu Division of Microbiology Entomology Research InstituteLoyola College Department of Botany and Microbiology College of ScienceKing Saud University

Objective:To isolate novel actinomycetes and to evaluate their antibacterial activity.Methods:Three soil samples were collected from Vengodu(village)in Kanchipuram district,Tamil Nadu,India.Actinomycetes were isolated using serial dilution and plating method on actinomycetes isolation agar.Results:Totally 35 isolates were obtained on the basis of colony characteristics on actinomycetes isolation agar.All the isolates were screened for antibacterial activity by cross streak method.Medium and optimization of day were done for the potent strains using Nathan's agar well diffusion method.Isolation of bioactive compounds from significant active isolates was done by using different media.The most active isolate VAs 10 was identified as Actinobacterium Loyola PBT VAs 10(accession No.JF501398)using 16s rRNA sequence method.The hexane,ethyl acetate,dichloromethane and butanol extracts of VAs 10 were tested against bacteria.The maximum antibacterial activity was observed in dichloromethane and ethyl acetate;maximum zones of inhibition were observed against Enterococcus durans.The rRNA secondary structure and the restriction sites of Actinobacterium Loyola VAs 10 were predicted using Genebee and NEBCutter online tools respectively.Conclusions:The present study showed that among the isolated actinomycetes,Actinobacterium Loyola PBT VAs 10(accession No.JF501398)showed good antibacterial activity against the tested bacteria.

关键词： VAs10 Actinobacterium Antibacterial activity solvent extract

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Ovicidal activity of Atalantia monophylla(L)Correa against spodoptera litura Fab.(Lepidoptera:Noctuidae)

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Asian Pacific Journal of Tropical Biomedicine 2012年第12期2卷 987-991页

作者： Kathirvelu Baskar Chellaiah Muthu Gnanaprakasam Antony Raj selvadurai Kingsley savarimuthu ignacimuthu Entomology Research Institute Loyola College PG and Research Department of Advanced Zoology and Biotechnology Loyola College

Objective:To evaluate the efficacy of Atalantia monophylla(A.monophylla)leaf in different solvent crude extracts and fractions against eggs of spodoptera litura(s.litura).Methods:Hexane,ethyl acetate and chloroform solvent extracts of A.monophylla leaf and 12 fractions from hexane extract were screened at 5.0%t,2.5%,1.0%and 0.5%for crude extracts and 1000,500,250 and 125 mg/kg for fractions against the eggs of s.litura for the ovicidal activity.LC_(50)and LC_(90)were calculated using probit analysis.Results:Hexane crude extract showed maximum ovicidal activity of 61.94%at 5.0%concentration with a correlation value of r^2=0.81,and least LC_(50)value of3.06%.Hexane extract was fractionated using silica gel column chromatography and 12 fractions were obtained.Fraction 9 was active which showed maximum ovicidal activity of 75.61%at 1000mg/kg with the LC_(50)value of 318.65 mg/kg and LC_(90)value of 1473.31 mg/kg.In linear regression analysis,significant and high correlation(r^2=0.81%)was seen between concentration and ovicidal activity of hexane crude extracts and its active frsctien.Conclusions:As per our knowledge,this is the first report for ovicidal activity of A.monophylla against s.litura,A.monophylla could be used for the management of s.litura and other insect pests.

关键词： Atalantia monophylla Crude extracts Fractions Ovicidal activity spodoptera litura

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