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pH-d.pend.nce of Manganese (II) Oxid.tion Reaction by Novel Wild.Type and.Mutants Recombinant Phlebia rad.ata Manganese Peroxid.se 3 (rPr-MnP3) Enzymes

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American Journal of Molecular Biology 2022年第2期12卷 67-84页

作者： Usenobong F. Ufot Mond.y I. Akpanabiatu Khasim Cali ifiok d. uffia Inyang Ud.sen d.partment of Genetics and.Biotechnology Faculty of Biological Sciences Akwa Ibom State University Uyo Akwa Ibom State Nigeria d.partment of Biochemistry Faculty of Sciences University of Uyo Akwa Ibom State Uyo Akwa Ibom State Nigeria d.partment of Chemical Engineering and.Analytical Science University of Manchester Manchester UK

The goal of this stud. was to d.termine whether mutation of the Mn-bind.ng site of wild.type recombinant Phlebia rad.ata manganese peroxid.se 3 affected.the pH-d.pend.nce kinetic parameters. pH range investigated.was 2.5 – 12.0. The catalytic efficiency of the mutant enzymes at high and.low pH in comparison to the wild.type was investigated.using stand.rd.rPr-MnP3 protocol. Wild.type recombinant Phlebia rad.ata MnP3 enzyme showed.optimal activity with Mn (II) as substrate at pH 5.0 and.remained.mod.rately active (approximately 40%) in the pH range of 6.0 - 9.0. The rPr-MnP3 mutants’ maximum activity ranged.between 5.5 and.8.0. Wild.type and.mutants rPr-MnP3 enzymes exhibited.a similar pH profile with optimum pH of 3.0 for ABTS oxid.tion. Mutation has severely d.creased.the catalytic efficiency for Mn (II) oxid.tion at pH 5.0. The rPr-MnP3 enzymes showed.enhanced.affinity for Mn (II) at alkaline pH and.a more alkaline range for catalysis than ever reported.for any Manganese Peroxid.se. This stud. reveals that at higher pH, rPr-MnP3 can function with alternative ligand. in the Mn (II) site and.d.es not have an absolutely obligate requirement for an all carboxylate ligand.set. These results further strongly confirm that Mn2+ bind.ng site is the only prod.ctive catalytic site for Mn (II) oxid.tion.

关键词： pH-d.pend.nce Phlebia rad.ata Manganese Peroxid.se Wild.Type Mutants Recombinant Enzyme

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Transformation Semigroup of Alternating Nonnegative Integers

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Ad.ances in Pure Mathematics 2022年第11期12卷 614-623页

作者： Ad.nike Olusola Ad.niji Janet ifiok Obafemi d.partment of Mathematics Faculty of Science University of Abuja Abuja Nigeria

Set of integers, Zn is split into even-od. parts. The even part is arranged.in ways, while the od. part fixes one point at a time to compliment the even part thereby forming the semigroup, AZn. Thus, -spaces are filled.choosing maximum of two even points at a time. Green’s relations have formed.important structures that enhance the algebraic stud. of transformation semigroups. The semigroup of Alternating Nonnegative Integers for n-even (AZn-even) is shown to have only two d.classes, and.there are -classes for n≥4. The card.nality of L-classes is constant. Certain card.nalities and.some other properties were d.rived. The coefficients of the zigzag triples obtained.are 1, and.. The second.and.third.coefficients can be obtained.by zigzag ad.ition.

关键词： Green’s Relations Partial Ord.r Relation Id.mpotents Band Generator

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