检索结果-南通市图书馆

Expressions of sonic hedgehog, patched, smoothened and Gli-1 in human intestinal stromal tumors and their correlation with prognosis

引用

World Journal of Gastroenterology 2006年第35期12卷 5687-5691页

作者： Ayumi Yoshizaki Toshiyuki Nakayama Shinji Naito Chun-Yang Wen ichiro sekine Department of Molecular Pathology Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Division of Pathology Research LaboratoryNational Ureshino Medical Center Saga Japan Department of Digestive Disease Affiliated Hospital of Beihua University Jilin 132011 Jilin Province China

AIM: To investigate the role that the hedgehog (Hh) signaling pathway, which includes sonic hedgehog (Shh), Patched (Ptc), Smoothened (Smo) and Gli-1, plays in hu- man gastrointestinal stromal tumors (GISTs). METHODS: Surgically resected specimens from pa- tients with GISTs, leiomyomas and schwannomas were examined by immunohistochemical staining for aberrant expression of hedgehog signaling components, Shh, Ptc, Smo and Gli-1, respectively. RESULTS: In GISTs, 58.1% (18 of 31), 77.4% (24 of 31), 80.6% (25 of 31) and 58.1% (18 of 31) of the specimens stained positive for Shh, Ptc, Smo and Gli-1, respectively. In leiomyomas, 92.3% (12 of 13), 92.3% (12 of 13), 69.2% (9 of 13) and 92.3% (12 of 13) stained positive for Shh, Ptc, Smo and Gli-1, respectively. In schwannomas, 83.3% (5 of 6), 83.3% (5 of 6), 83.3% (5 of 6) and 100% (6 of 6) stained positive for Shh, Ptc, Smo and Gli-1, respectively. Immunohistochemistry revealed that the expressions of Shh and Gli-1 were sig- nificantly higher in leiomyomas than in GISTs (P < 0.05, respectively). Shh expression strongly correlated with the grade of tumor risk category and with tumor size (P < 0.05, respectively). However, the expressions of Ptc and Smo did not correlate with histopathological differentiation. CONCLUSION: These results suggest that the Hh sig- naling pathway may play an important role in myogenic differentiation and the malignant potential of human in-testinal stromal tumors.

关键词： Gastrointestinal stromal tumor Leiomyoma Schwannoma Hedgehog Immunohistochemistry

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of vascular endothelial growth factor and its receptors VEGFR-1 and 2 in gastrointestinal stromal tumors,leiomyomas and schwannomas

引用

World Journal of Gastroenterology 2006年第38期12卷 6182-6187页

作者： Toshiyuki Nakayama Yang Cheul Cho Yuka Mine Ayumi Yoshizaki Shinji Naito Chun Yang Wen ichiro sekine Department of Tumor and Diagnostic Pathology Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Division of Pathology Research Laboratory National Ureshino Medical Center Saga 843-0301 Japan Department of Digestive Disease Affiliated hospital of Beihua University Jilin 132011 Jilin Province China

AIM： To investigate the role of vascular endothelial growth factor （VEGF/and its receptors VEGFR-1 and 2 in the growth and differentiation of gastrointestinal strornal tumors （GISTs）. METHODS： Thirty-three GISTs, 15 leiomyomas and 6 schwannomas were examined by immunohistochemistry in this study. RESULTS： VEGF protein was expressed in the cytoplasm of tumor cells, and VEGFRol and 2 were expressed both in the cytoplasm and on the membrane of all tumors. Irnrnunohistochernical staining revealed that 26 GISTs （78.8%）, 9 leiornyornas （60.0%） and 3 schwannornas （50.0%/were positive for VEGF; 24 GISTs （72.7%/, 12 leiornyornas （80.0%） and 4 schwannornas （66.7%） were positive for VEGFR-1; 30 GISTs （90.9%/, 5 leiornyornas （33.3%/and 4 schwannornas （66.7%） were positive for VEGFR-2. VEGFR-2 expression was statistically different between GISTs and leiomyomas （P 〈 0.0001）. However, there was no correlation between the expression of VEGF pathway componenets and the clinical risk categories. CONCLUSION： Our results suggest that the VEGF pathway may play an important role in the differentiation of GISTs, leiomyomas and schwannomas.

关键词： Gastrointestinal stromal tumor Leiomyoma Schwannoma Vascular endothelial growth factor Vascular endothelial growth factor receptors

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of Ets-1 proto-oncoprotein in gastrointestinal stromal tumors, leiomyomas and schwannomas

引用

World Journal of Gastroenterology 2006年第11期12卷 1743-1746页

作者： Toshiyuki Nakayama Ayumi Yoshizaki Shinji Naito Chun Yang Wen Gabit Alipov Yuichi Yakata ichiro sekine Department of Molecular Pathology Nagasaki University Graduate School of Biomedical Sciences1-12-4 Sakamoto Nagasaki 852-8523 Japan Division of Pathology Research Laboratory National Ureshino Medical Center Saga 843-0301 Japan Department of Digestive Disease Nanjing Drum Tower Hospital Medical School of Nanjing UniversityNanjing 210008 Jiangsu Province China Tissue and Histopathology Section Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Sciences Nagasaki 852-8523 Japan

AIM： Gastrointestinal stromal tumors （GISTs） are rare. GISTs differ from other mesenchymal tumors of the gastrointestinal tract （e.g. leiornyomas and schwannomas）. The purpose of this study was to investigate the role of Ets-1 in the growth and differentiation of GISTs. METHODS： Twenty-eight GISTs, nine leiomyomas and six schwannomas were examined by immunohistochemical staining method for Ets-1 in this study. Specimens were selected from surgical pathology archival tissues at Nagasaki University Hospital. RESULTS： Ets-1 protein was expressed in the cytoplasm of cells in all of these tumors. Immunohistochemical staining revealed that 27 GISTs （96.4%）, six leiomyomas （66.7%）, and five schwannomas （83.3%） were positive for Ets-1. Ets-1 expression was statistically different between GISTs and leiomyomas （P〈0.005）. However, there was no correlation between Ets-1 expression and clinical risk categories. CONCLUSION： Ets-1 plays an important role in the growth and differentiation of GISTs, leiomyomas and schwannomas.

关键词： Ets-1 GISTs Leiomyoma Schwannoma Immunohistochemistry Risk category

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Antibody to eosinophil cationic protein suppresses dextran sulfate sodium-induced colitis in rats

引用

World Journal of Gastroenterology 2005年第29期11卷 4505-4510页

作者： Kazuko Shichijo Kazuya Makiyama Chun-Yang Wen Mutsumi Matsuu Toshiyuki Nakayama Masahiro Nakashima Makoto Ihara ichiro sekine Department of Molecular Pathology Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Department of Endoscopy Nagasaki University School of Medicine Hospital and Clinics 1-12-4 Sakamoto Nagasaki 852-8523 Japan Department of Digestive Disease the Affiliated Drum Tower Hospital of Nanjing University Medical School Nanjing 210008 Jiangsu Province China Tissue and Histopathology Section Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Radiation Biophysics Atomic Bomb Disease Institute Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan

AIM： To produce an antibody against rat eosinophil cationic protein （ECP） and to examine the effects of the antibody in rats with dextran sulfate sodium （DSS）-induced colitis. METHODS： An antibody was raised against rat ECP. Rats were treated with 3% DSS in drinking water for 7 d and received the antibody or normal serum. The colons were examined histologically and correlated with clinical symptoms. Immunohistochemistry and Western blot analysis were estimated as a grade of inflammation. RESULTS： The ECP antibody stained the activated eosinophils around the injured crypts in the colonic mucosa. Antibody treatment reduced the severity of colonic ulceration and acute clinical symptoms （diarrhea and/or bloodstained stool）. Body weight gain was significantly greater and the colon length was significantly longer in anti-ECPtreated rats than in normal serum-treated rats. Expression of ECP in activated eosinophils was associated with the presence of erosions and inflammation. The number of Ki-67-positive cells in the regenerated surface epithelium increased in anti-ECP-treated rats compared with normal serum-treated rats. Western blot analysis revealed reduced expression of macrophage migration inhibitory factor （MIF） in anti-ECP-treated rats. CONCLUSION： Our results indicate that treatment with ECP antibody, improved DSS-induced colitis in rats, possibly by increasing the regenerative activity of the colonic epithelium and downregulation of the immune response, and suggest that anti-ECP may promote intestinal wound healing in patients with ulcerative colitis （UC）.

关键词： Ulcerative colitis Eosinophil cationic protein Dextran sulfate sodium

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of Angiopoietin-1, 2 and 4 and Tie-1 and 2 in gastrointestinal stromal tumor, leiomyoma and schwannoma

引用

World Journal of Gastroenterology 2007年第33期13卷 4473-4479页

作者： Toshiyuki Nakayama Maki Inaba Shinji Naito Yumi Mihara Shiro Miura Mitsuru Taba Ayumi Yoshizaki Chun-Yang Wen ichiro sekine Department of Tumor and Diagnostic Pathology Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Division of Pathology Research Laboratory National Ureshino Medical Center Saga 843-0301 Japan Department of Digestive Disease Affiliated hospital of Beihua University Jilin 132011 Jilin Province China

AIM: To investigate the role of angiopoietin (Ang) -1, -2 and -4 and its receptors, Tie-1 and -2, in the growth and differentiation of gastrointestinal stromal tumors (GISTs).METHODS: Thirty GISTs, seventeen leiomyomas and six schwannomas were examined by immunohistochemistry in this ***: Ang-1, -2 and -4 proteins were expressed in the cytoplasm of tumor cells, and Tie-1 and -2 were expressed both in the cytoplasm and on the membrane of all tumors. Immunohistochemical staining revealed that 66.7% of GISTs (20 of 30), 76.5% of leiomyomas (13 of 17) and 83.3% of schwannomas (5 of 6) were positive for Ang-1. 83.3% of GISTs (25 of 30), 82.4% of leiomyomas (14 of 17) and 100% of schwannomas (6 of 6) were positive for Ang-2. 36.7% of GISTs (11 of 30), 58.8% of leiomyomas (10 of 17) and 83.3% of schwannomas (5 of 6) were positive for Ang-4. 60.0% of GISTs (18 of 30), 82.4% of leiomyomas and 100% of schwannomas (6 of 6) were positive for Tie-1. 10.0% of GISTs (3 of 30), 94.1% of leiomyomas (16 of 17) and 33.3% of schwannomas (2 of 6) were positive for Tie-2. Tie-2 expression was statistically different between GISTs and leiomyomas (P < 0.001). However, there was no correlation between expression of angiopoietin pathway components and clinical risk ***: Our results suggest that the angiopoietin pathway plays an important role in the differentiation of GISTs, leiomyomas and schwannomas.

关键词： Gastrointestinal stromal tumors Leiomyomas Schwannomas

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Expression of Interleukin-11 and Interleukin-11 receptor a in human colorectal adenocarcinoma; Immunohistochemical analyses and correlation with clinicopathological factors

引用

World Journal of Gastroenterology 2006年第2期12卷 317-321页

作者： Kazuyuki Yamazumi Toshiyuki Nakayama Takafumi Kusaba Chun Yang Wen Ayumi Yoshizaki Yuichi Yakata Takeshi Nagayasu ichiro sekine Department of Molecular Pathology Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Division of Surgical Oncology Department of Translational Medical Sciences Nagasaki University Graduate School of Biomedical Sciences 1-12-4 Sakamoto Nagasaki 852-8523 Japan Department of Digestive Disease Nanjing Drum Tower Hospital Medical School of Nanjing University Nanjing 210008 Jiangsu Province China

AIM： There is strong evidence that interleukin-11 （IL-11） is involved in the regulation of tumor progression, cellular growth and differentiation. Recently, interleukin-11 receptor （IL-11R） has been detected on some cancer cells. In this study, we investigated the expression of IL-11 and IL-11R in colorectal adenocarcinoma. METHODS： To elucidate the involvement of IL-11 and IL-11Rα in human intestinal adenocarcinomas, we examined 115 cases of surgically resected human colonic adenocarcinoma and 11 cases of adenoma by immunohistochemistry and Western blotting. RESULTS： Among 115 cases of adenocarcinoma, 100 cases （87.0%） showed positive staining in the cytoplasm of carcinoma cells for the IL-11, and 87 cases （75.6%） were positive for the IL-11Ra. Six cases （54.5%） and four cases （36.4%） of 11 adenomas were positive for IL-11 and IL-11Ra, respectively. The expression of IL- 11Ra correlated with the histological differentiation （P=0.033503）, the depth of tumor invasion （P= 0.006395）, Dukes＇ classification （P= 0.015648） and lymphatic invasion （P= 0.003865）. However, the expression of IL- 11Rα was not correlated with the venous invasion and the presence of lymph node metastasis. The expression of IL-11 was not correlated with any clinicopathological factors. In Western blot analysis, two human colorectal carcinoma cell lines and four tissues of surgically resected human carcinoma expressed both IL-11 and IL-11Rα proteins. CONCLUSION： IL-11 and IL-11Rα are highly expressed in human colorectal adenocarcinoma and the IL-11Rα expression is correlated with clinicopathological factors, These findings suggest that the expression of IL-11Rα is an important factor for the invasion of human colorectal adenocarcinoma.

关键词： IL-11, IL-11 receptor α Colorectal cancer

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

强酸性电解质水冲洗根管的清洁效果

引用

牙体牙髓牙周病学杂志 2007年第4期17卷 210-213页

作者：余擎秋田康充吉田隆一關根一郎第四军医大学口腔医学院陕西西安710032 日本朝日大学齿学部保存齿科日本歧阜瑞穗5010296

目的:体外观察比较强酸性电解质水与其他3类冲洗剂及其方法在根管治疗术中的清洁效果。方法:40个新鲜拔除的单根、单根管牙,常规逐步后退法进行根管预备,按照不同的冲洗组合和方法随机分为5组进行根管预备后的冲洗,然后纵向劈开牙齿,扫... 详细信息

目的:体外观察比较强酸性电解质水与其他3类冲洗剂及其方法在根管治疗术中的清洁效果。方法:40个新鲜拔除的单根、单根管牙,常规逐步后退法进行根管预备,按照不同的冲洗组合和方法随机分为5组进行根管预备后的冲洗,然后纵向劈开牙齿,扫描电镜观察根管壁的情况。结果:强酸性电解质水、EDTA均能有效去除根管玷污层,冲洗1 min、3 min与150 g/L EDTA冲洗1 min没有显著差别,前者与EDTA加次氯酸钠液配合使用组相比,根管壁的玷污层、细菌均得到有效清除。结论:强酸性电解质水结合超声振荡能有效地清除根管玷污层和细菌。

关键词：根管冲洗根管治疗术玷污层扫描电镜

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Measurement Method of Internal Residual Stress in Plastic Parts Using Terahertz Spectroscopy

引用

Nanomanufacturing and Metrology 2021年第1期4卷 46-52页

作者： Yusuke Kajihara Rio Takahashi ichiro Yoshida Shingo Saito Norihiko sekine Shuichi Nowatari Shigeo Miyake Institute of Industrial Science The University of TokyoKomaba 4-6-K Meguro-kuTokyoJapan Hosei University Kajino 3-7-2.KoganeiTokyoJapan National Institute of Information and Communications Technology Nukui-Kitamachi 4-2-1KoganeiTokyoJapan Iida Co. LtdNukazawa Mizukami 21-2MotomiyiaFukushimaJapan

Since internal residual stress in polymers causes dimension errors over time,a measurement method for residual stress in polymers,especially in opaque engineering plastics,is strongly *** this study,we propose to utilize terahertz(THz)spectroscopy to non-destructively measure internal residual stress in *** verify the validity of the proposed method,we manufactured polytetrafluoroethylene ring samples with and without residual stress,and studied them with THz time domain spectroscopy *** spectra of samples without residual stress showed almost no polarization difference,whereas spectra of samples with residual stress showed clear polarization *** changes between the samples with and without residual stress were also estimated and it was found that the change had a correlation with THz polarization *** experimental analyses confirm the validity for the proposed non-destructive measurement of residual stress in polymer.

关键词： Engineering plastics Residual stress Polarization Terahertz spectroscopy

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Fatal submucosal invasive gastric adenosquamous carcinoma detected at surveillance after gastric endoscopic submucosal dissection

引用

World Journal of Gastroenterology 2015年第14期21卷 4385-4390页

作者： Akinori Shirahige Haruhisa Suzuki ichiro Oda Masau Sekiguchi Genki Mori Seiichiro Abe Satoru Nonaka Shigetaka Yoshinaga Shigeki sekine Ryoji Kushima Yutaka Saito Takeo Fukagawa Hitoshi Katai Endoscopy Division National Cancer Center Hospital Pathology Division National Cancer Center Hospital Department of Clinical Laboratory Medicine and Diagnostic Pathology Shiga University of Medical Science Gastric Surgery Division National Cancer Center Hospital

An 80-year-old man was under annual surveillance esophagogastroduodenoscopy after endoscopic submucosal dissection(ESD) for early gastric cancer(EGC).Two years after the initial ESD, a 0-Ⅱc type metachronous EGC lesion, 8 mm in size, without an ulcer scar, was found in the gastric *** estimated tumor depth was up to the mucosa, and biopsy revealed well and poorly differentiated *** was performed for this lesion and en bloc resection with negative margins was *** examination revealed an adenosquamous carcinoma 8 mm in size invading the deep submucosal layer(1600 μm), with lymphovascular invasion, consistent with the diagnosis of non-curative *** gastrectomy was recommended for this patient; however, two months after the ESD, preoperative computed tomography revealed multiple liver metastases, and the patient was considered as an unsuitable candidate for surgical *** chemotherapy was therefore started; however, the patient died of gastric cancer 27 mo after the second *** gastric adenosquamous carcinoma localized to the mucosa and submucosa is extremely rare and its clinical behavior is not well *** present report is very significant in that it underscores the distinct possibility of gastric adenosquamous carcinoma being very aggressive and fatal even when detected at an early cancer.

关键词： Early gastric cancer Endoscopic submucosal dissect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：