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过敏性疾病患者过敏原特异性IgE检测分析

国际检验医学杂志 2015年第19期36卷 2779-2781页

作者：黄燕华张秀明王伟佳中山大学附属中山医院检验医学中心广东中山528403

目的探讨过敏性疾病患者常见过敏原特异性IgE阳性分布特征。方法选择该院2014年3~10月疑似过敏性疾病患者1 884例,采用荧光酶联免疫吸附法有针对性地对其血清进行4种吸入性过敏原组合（尘螨类、霉菌类、动物表皮类、花粉类）和4种食入... 详细信息

目的探讨过敏性疾病患者常见过敏原特异性IgE阳性分布特征。方法选择该院2014年3~10月疑似过敏性疾病患者1 884例,采用荧光酶联免疫吸附法有针对性地对其血清进行4种吸入性过敏原组合（尘螨类、霉菌类、动物表皮类、花粉类）和4种食入性过敏原组合（海鲜类、谷类、肉类、奶制品）的特异性IgE检测,分析各类过敏原组合的特异性IgE表达情况,并观察过敏患者特异性IgE的性别与年龄分布差异。结果吸入性过敏原中尘螨类占73.9%、动物表皮类8.5%、霉菌类6.5%、花粉类4.0%;食入性过敏原中海鲜类占47.8%、奶制品31.5%、谷类23.0%、肉类8.5%。同时有2种或2种以上混合过敏原阳性的过敏性疾病患者高达82.0%,且尘螨类和动物表皮类常混合致敏。不同性别过敏原阳性率差异无统计学意义（P〉0.05）。过敏原特异性IgE阳性率随着年龄增加而降低。结论尘螨类是主要的吸入性过敏原组合;海鲜类、奶制品和谷类是主要的食入性过敏原组合,过敏患者多见混合过敏原致敏,随着年龄增长过敏原阳性率逐渐降低。

关键词：过敏原特异性IgE 荧光酶联免疫吸附法

羊口疮病毒b2L基因DNA疫苗的构建及其诱导的免疫应答

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中国兽医学报 2018年第4期38卷 704-708,715页

作者：李鹏飞冯将鲜思美刘嫒李婷包细明张益张素辉贵州大学动物科学学院贵州贵阳550025 贵州省动物疫病研究所贵州贵阳550025 重庆市畜牧科学院重庆荣昌402460

为研究所构建羊口疮病毒（OrfV）b2L基因DNA疫苗诱导小鼠的免疫应答效果，本研究对pMD18T-b2L质粒进行PCR扩增，克隆b2L片段至pVAX1载体中构建pVAX1-b2L重组质粒，进行酶切和测序鉴定；采用脂质体法将pVAX1-b21。真核表达质粒转染MDbK... 详细信息

为研究所构建羊口疮病毒（OrfV）b2L基因DNA疫苗诱导小鼠的免疫应答效果，本研究对pMD18T-b2L质粒进行PCR扩增，克隆b2L片段至pVAX1载体中构建pVAX1-b2L重组质粒，进行酶切和测序鉴定；采用脂质体法将pVAX1-b21。真核表达质粒转染MDbK细胞，RTPCR和IFA法检测b2L基因在MDbK细胞中的转录和表达；将构建的DNA疫苗免疫KM系小鼠，采用间接ELISA、MTT和FACS法对其诱导的免疫应答进行研究。结果显示，成功构建pVAX1-b2L真核表达质粒，并在MDbK细胞中表达；免疫小鼠后，DNA疫苗能诱导小鼠产生Orfv特异性抗体；脾淋巴细胞增殖、CD4^＋、CD8^＋T淋巴细胞亚群百分比和IL-2、IFN-γ、IL-4细胞因子均高于pVAX1组和PbS组。结果表明，本研究制备的DNA疫苗能够诱导小鼠产生较高水平的体液免疫和细胞免疫应答。

关键词：羊口疮病毒(OrfV) b2L基因 DNA疫苗体液免疫细胞免疫

吡柔比星与α-干扰素联用预防膀胱癌术后复发有效性及安全性的Meta分析

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现代泌尿生殖肿瘤杂志 2015年第6期7卷 335-339页

作者：吴昊窦中岭贾招辉李帅阳河南科技大学第一附属医院泌尿外科洛阳471003

目的评价吡柔比星与α-干扰素联用预防膀胱癌术后复发的有效性及安全性。方法遵循循证医学的原则,计算机检索PubMed、中文期刊全文数据库(CNKI)和万方数据库,纳入符合入选标准的关于吡柔比星与α-干扰素联用预防膀胱癌术后复发有效性及... 详细信息

目的评价吡柔比星与α-干扰素联用预防膀胱癌术后复发的有效性及安全性。方法遵循循证医学的原则,计算机检索PubMed、中文期刊全文数据库(CNKI)和万方数据库,纳入符合入选标准的关于吡柔比星与α-干扰素联用预防膀胱癌术后复发有效性及安全性的随机对照试验的文献并评价质量,应用RevMan 5.1对纳入研究进行异质性检验和效应值OR合并。结果 1共纳入5个符合要求的病例-对照研究,包括327例患者,其中吡柔比星与α-干扰素联用162例,单用吡柔比星165例;2根据随机方法、分配隐藏、盲法、退出或失访、意向性分析和基线情况的描述对纳入研究进行质量评分,结果质量均为b级;3Meta分析结果显示随访1年时吡柔比星与α-干扰素联用膀胱癌术后复发率与单用吡柔比星比较,OR=0.23,P0.05,尚不能认为两组差异有统计学意义。结论吡柔比星与α-干扰素联用组的术后复发率较单用吡柔比星组低、效果好。

关键词：膀胱肿瘤吡柔比星干扰素 Meta分析

Preparation of diffusion coatings in ion-electron melts

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广东有色金属学报 2005年第2期15卷 467-470页

作者： Anfinogenov A.I . Chebykin V.V. Chernov Ya .b. Institute of High-Temperature Electrochemistry Ural Division of RAS Ekaterinburg 620219 Russia

A procedure of Ni, Cr, Mn, Co, and Mo (Me) transfer onto iron substrate in ionic-electronic melts LiCl-Li, CaCl2-Ca, and baCl2-ba was elaborated and the transport processes were studied. The saturated vapor pressure of these ionic-electronic melts is as low as enabled working at atmosphere pressure in an inert media up to 1000 ℃.Armco iron was used as a substrate because it practically does not interact with lithium, calcium, and barium. The metals-diffusants were put into the melts in the form of a powder. The complete saturation of the melts with lithium, calcium, and barium was ensured by a preceding contact of a molten salt with an excess of Li, Ca, or ba before loading of *** reactions take place despite the metals and iron were separated from each other by the molten salt. The quantity of the metals transfered was determined by the change in mass before and after experiments, by microprobe analysis, and x-raying of the iron surface layer. The experiments were carried out at 900, 950 and 1000℃ during 5, 10, 15 and 20 *** the first stage of the process the formation of the ionic-electronic melt occurs. For example, dissolution of calcium in the case of the system CaCl2-Ca is mainly proceeded in the form of one-valent cations: Ca + Ca2+()Ca+. As soon as the metal-diffusance is immersed into the melt, its dissolution in the form of negative ions takes place: Me + Ca+() Me-+ Ca2+. In the vicinity of the iron substrate the last equilibrium will shift to the left generating a solid solution or intermetallide. The thickness of the diffusion layer rises as the temperature and saturation time increase. The obtained coverings were 20-60 (m thick,and their surface layer contained 15%-80% (mass fraction) of the *** the composition of the melt and the procedure of the coverings preparation in ionic-electronic melts have been patented (Patent RU2058422, 1996, pr. 30.09.93).

关键词：金属材料表面处理涂层电化学电子-离子融化法

A Role for GIbbERELLIN 2-OXIDASE6 and Gibberellins in Regulating Nectar Production

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Molecular Plant 2016年第5期9卷 753-756页

作者： Lisa b. Wiesen Ricci L. bender Travis Paradis Alexie Larson M. Ann D.N. Perera basil J. Nikolau Neil E. Olszewski Clay J. Carter Department of Plant biology University of Minnesota Saint PaulMN 55108 USA Department of biochemistry Biophysics & Molecular Biology Iowa State University Ames IA 50011 USA W.M.Keck Metabolomics Research Laboratory Iowa State University Ames IA 50011 USA Department of Plant biology University of Minnesota Twin Cities 1445 Gortner Avenue 250 Biological Sciences Center Saint Paul MN 55108 USA

Dear Editor The regulation of nectar production is dependent on hormonal signaling. For example, Arabidopsis PIN6, which encodes an auxin efflux transporter family protein, was identified as a nectary-enriched gene whose expression level was positively correlated to total nectar production （bender et al., 2013）. Furthermore, jasmonic acid （JA） levels peak in brassica flowers just prior to anthesis and promote nectar secretion （Radhika et al., 2010）. While auxin and JA have been demonstrated to play roles in the regulation of nectar production,

关键词： GIbbERELLIN Gibberellins Production

Intra-arterial thrombolytics during endovascular thrombectomy for acute ischaemic stroke in the MR CLEAN Registry

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Stroke & Vascular Neurology 2023年第1期8卷 17-25页

作者： Sabine L Collette Reinoud P H bokkers Aryan Mazuri Geert J LycklamaàNijeholt Robert J van Oostenbrugge Natalie E LeCouffe Faysal benali Charles b L M Majoie Jan Cees de Groot Gert Jan R Luijckx Maarten Uyttenboogaart 无 Department of Radiology Medical Imaging CentreUniversity Medical Centre GroningenUniversity of GroningenGroningenthe Netherlands Department of Radiology Haaglanden Medical CentreThe Haguethe Netherlands Department of Neurology Cardiovascular Research Institute Maastricht(CARIM)Maastricht University Medical CentreMaastrichtthe Netherlands Department of Neurology Amsterdam UMCUniversity of AmsterdamAmsterdam NeuroscienceAmsterdamthe Netherlands Department of Radiology Maastricht University Medical CentreMaastrichtthe Netherlands Department of Radiology and Nuclear Medicine Amsterdam UMCUniversity of AmsterdamAmsterdam NeuroscienceAmsterdamthe Netherlands Department of Neurology University Medical Centre GroningenUniversity of GroningenGroningenthe Netherlands On behalf of the MR CLEAN Registry investigators

Introduction The efficacy and safety of local intra-arterial(ia)thrombolytics during endovascular thrombectomy(EVT)for large-vessel occlusions is *** analysed how often ia thrombolytics were administered in the Multicenter Randomized Clinical Trial of Endovascular Treatment for Acute Ischemic Stroke in the Netherlands(MR CLEAN)Registry,whether it was associated with improved functional outcome and assessed technical and safety outcomes compared with EVT without ia *** In this observational study,we included patients undergoing EVT for an acute ischaemic stroke in the anterior circulation from the MR CLEAN Registry(March 2014-November 2017).The primary endpoint was favourable functional outcome,defined as an modified Rankin Scale score≤2 at 90 *** endpoints were reperfusion status,early neurological recovery and symptomatic intracranial haemorrhage(sICH).Subgroup analyses for ia thrombolytics as primary versus adjuvant revascularisation attempt were *** Of the 2263 included patients,95(4.2%)received ia thrombolytics during *** ia thrombolytics administered were urokinase(median dose,250000 IU(IQR,193750-250000))or alteplase(median dose,20 mg(IQR,12-20)).No association was found between ia thrombolytics and favourable functional outcome(adjusted OR(aOR),1.16;95%CI 0.71 to 1.90).Successful reperfusion was less often observed in those patients treated with ia thrombolytics(aOR,0.57;95%CI 0.36 to 0.90).The odds of sICH(aOR,0.82;95%CI 0.32 to 2.10)and early neurological recovery were comparable between patients treated with and without ia *** primary and adjuvant revascularisation attempts,ia thrombolytics were more often administered for proximal than for distal *** outcomes were comparable for patients receiving ia thrombolytics as a primary versus adjuvant revascularisation *** Local ia thrombolytics were rarely used in the MR CLEAN *** the relatively small st

关键词： arterial throm acute

Distributed Control for Optimal Economic Dispatch of Power Generators

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Distributed Control for Optimal Economic Dispatch of Power G...

In Silico Prediction Structure and Function for Novel Protein Alkane Hydroxylase the Pseudomonas Nitroreducens

第二十九届中国控制会议

作者： R.Mudumbai S.Dasgupta b.Cho Department of Electrical and Computer Engineering University of IowaIowa CityIA 52242

In this paper,we present a simple,distributed algorithm that adjusts the power-frequency set-points of generators to correct for generation and load fluctuations,and asymptotically achieves the lowest-cost power allocation among generators while driving the power imbalance exponentially to *** this algorithm each generator executes its own control law using only the power imbalance in the network,a quantity in turn locally obtained through local monitoring of the frequency deviation on the *** results only assume that the cost of each generator is a convex *** distributed approach is motivated by the anticipated needs of the future electric grid with a large penetration of distributed small-scale alternative energy generators,and also consumers with "smart metering" *** present analytical results and numerical simulations to illustrate the performance of our algorithm.

关键词： Distributed Energy Smart Grids Optimal Dispatch

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材料科学与工程（中英文A版） 2014年第7期4卷 241-248页

作者： Silvana Pomp6ia Val-Moraes Gisele Regina Rodrigues Douglas Antonio Alvaredo Paixào, Eliana Gertrudes Macedo Lemos Department Technology LBMP Via de Acesso Prof. Paulo Donato Castellane s/n Univ. Estadual Paulista UNESP/FCAVJabotieabal S6o Paulo Brazil Laborat6rio Nacional de Ciência e Tecnologia do bioetanol (CTbE) Rua Giuseppe Màximo Scalfaro Sao Paulo Brazil

Currently in brazil is used the b5 fuel composed of 95% of diesel oil and 5% biodiesel. It has a mixture of alkanes, alkenes and aromatic compounds. The high sulfur content in the fuel determines brazilian great cost to the public health system. There is a 1.5% increase in hospitalizations for ischemic disease heart in the elderly and more than 4% for lung diseases in children and the elderly. Polycyclic aromatic hydrocarbons derived refined petroleum products are ubiquitous environmental due contamination of soil arising from spills or leaks most inhibit factors to soil fertility, crop productivity may bio-accumulate in food chains thus causing carcinogenesis of some organs and mutagenesis in the genetic material. In this work, analyzed the novel alkane hydroxylase described for Pseudomonas nitroreducens registered in Genebank as alkb gene (KJ023684.1). These analyses identified the protein described as alkane hydroxylase, superfamily and function, secondary structures, the phosphorylation sites, the protein GRAVY (grand average of hydropathy) and protein isoelectric point. The binding site is di-iron. This suggested that novel alkane hydroxylase deserve special attention to continue our work in situ, will be enzyme characterization and biosurfactants production or bioremediation have potential uses in environmental monitoring.

关键词：假单胞菌二级结构羟化酶蛋白质烷烃公共卫生体系建设生物表面活性剂预测

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肝脏神经内分泌肿瘤的超声表现

中华超声影像学杂志 2018年第8期27卷 698-703页

作者：林晓娜陈洁谢晓燕林满霞黄光亮张雨徐明吕明德中山大学附属第一医院超声医学科广州510080 中山大学附属第一医院消化内科广州510080

目的分析肝脏神经内分泌肿瘤（HNEN）的常规超声及超声造影特征，评价超声诊断及鉴别HNEN的价值。方法对50例经病理或影像确诊的HNEN患者行常规超声及超声造影检查，其中原发性HNEN（PHNEN）5例，转移性HNEN（MHNEN）45例。分析HNEN的... 详细信息

目的分析肝脏神经内分泌肿瘤（HNEN）的常规超声及超声造影特征，评价超声诊断及鉴别HNEN的价值。方法对50例经病理或影像确诊的HNEN患者行常规超声及超声造影检查，其中原发性HNEN（PHNEN）5例，转移性HNEN（MHNEN）45例。分析HNEN的常规超声及超声造影特点，并比较PHNEN和MHNEN的差异。结果常规超声示37例（74％）HNEN为肝内多发病灶，23例（46％）高回声，35例（70％）回声均匀，28例（56％）边界清楚，8例（16％）病灶周边伴声晕，12例（24％）病灶伴后方回声衰减。超声造影示大部分病灶呈“快进快出”的增强模式，动脉期46例（92％）HNEN呈高增强，门脉期44例（88％）消退为低增强，延迟期47例（94％）呈低增强。特殊征象中，31例（62％）HNEN可见肿瘤供血血管，17例（34％）出现瘤内坏死无增强区，10例（20％）可见包膜增强。而在PHNEN和MHNEN鉴别方面，MHNEN病灶比PHNEN更小[（392±2．77）cm对（12．82±8．85）cm，P=0．004]，较少出现囊性结构（P〈0．001），其余常规超声特点的差异均无统计学意义（P〉0．05）。PHNEN和MHNEN在超声造影各时相增强特点及增强时间的差异均无统计学意义（P〉0．05）。结论HNEN的超声表现具有一定特征性，超声检查对HNEN的诊断具有一定的价值。PHNEN和MHNEN的超声表现类似，鉴别诊断困难。

关键词：超声检查超声造影肝肿瘤神经内分泌肿瘤