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World Journal of Stem Cells 2017年第10期9卷 179-186页

作者： Harumichi itoh Shimpei Nishikawa Tomoya Haraguchi Yu Arikawa Shotaro Eto Masato Hiyama Toshie Iseri Yoshiki itoh Munekazu Nakaichi Yusuke Sakai Kenji Tani Yasuho Taura Kazuhito Itamoto Department of Small Animal Clinical Science Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Surgery Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Radiology Joint Faculty of Veterinary Medicine Yamaguchi University Department of Veterinary Pathology Joint Faculty of Veterinary Medicine Yamaguchi University

AIMTo identify and characterize functionally distinct subpopulation of adipose-derived stem cells (ADSCs). METHODSADSCs cultured from mouse subcutaneous adipose tissue were sorted fluorescence-activated cell sorter based on aldehyde dehydrogenase (ALDH) activity, a widely used stem cell marker. Differentiation potentials were analyzed by utilizing immunocytofluorescece and its quantitative analysis. RESULTSApproximately 15% of bulk ADSCs showed high ALDH activity in flow cytometric analysis. Although significant difference was not seen in proliferation capacity, the adipogenic and osteogenic differentiation capacity was higher in ALDHHi subpopulations than in ALDHLo. Gene set enrichment analysis revealed that ribosome-related gene sets were enriched in the ALDHHi subpopulation. CONCLUSIONHigh ALDH activity is a useful marker for identifying functionally different subpopulations in murine ADSCs. Additionally, we suggested the importance of ribosome for differentiation of ADSCs by gene set enrichment analysis.

关键词： Adipose-derived stem/stromal cell Aldehyde dehydrogenase activity Flow cytometry Subpopulation Ribosome

Streamline pair selection for comparative flow field visualization

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Visual Computing for Industry,Biomedicine,and Art 2020年第1期3卷 215-226页

作者： Shoko Sawada Takayuki itoh Takashi Misaka Shigeru Obayashi Tobias Czauderna Kingsley Stephens Ochanomizu University 2-1-1 OtsukaBunkyo-kuTokyo 1128610Japan Tohoku University 2-1-1 KatahiraAoba-kuSendaiMiyagi 9808577Japan Monash University Wellington RoadClaytonVictoriaAustralia

Fluid dynamics simulation is often repeated under varying *** leads to a generation of large amounts of results,which are difficult to *** compare results under different conditions,it is effective to overlap the streamlines generated from each condition in a single three-dimensional *** is a curved line,which represents a wind *** paper presents a technique to automatically select and visualize important streamlines that are suitable for the comparison of the simulation ***,we present an implementation to observe the flow fields in virtual reality spaces.

关键词： Computational fluid dynamics Streamline selection Comparative flow field visualization Virtual reality

Effect of wood density and water permeability on wood decomposition rates of 32 bornean rainforest trees

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Journal of Plant Ecology 2014年第4期7卷 356-363页

作者： Sanae Mori Akira itoh Satoshi Nanami Sylvester Tan Lucy Chong Takuo Yamakura Laboratory of Plant Ecology Graduate School of ScienceOsaka City UniversitySugimoto 3-3-138Sumiyoshi-kuOsaka 558-8585Japan Center for Tropical Forest Science Smithsonian Tropical Research Institute and the Arnold ArboretumHarvard University22 Divinity AvenueCambridgeMA 02138USA Applied Forest Science and Industry Development Sarawak Forestry CorporationJalan TapangKota SentosaKuching 93250SarawakMalaysia

Aims a better understanding of wood litter decomposition is essential for predicting responses of forest ecosystems to global climate *** studies suggest that chemical properties of wood litters,rather than physical ones such as wood density,are more important for inter-specific differences in wood decomposition ***,empirical data are still limited,especially for tropical *** addition,decom-position rate of wood litter often varies with time,which makes inter-specific comparison *** studied the wood decomposition of 32 rainforest trees to elucidate(i)the degree of interspecific variation in wood decomposition rate of a given size and configuration and(ii)if initial wood density and water permeability are consistent predictors of the overall decomposition rate and its pattern over *** a common garden decomposition experiment was conducted in a tropical rainforest in malaysian borneo for 32 native tree *** wood sticks were set on the forest floor and the weight loss was monitored monthly for 2.7 *** Findings We found large variation in the wood decomposition rate(a 49-fold range),suggesting that we need to consider this variation when cal-culating community-level carbon dynamics of tropical rain *** physical traits of wood,*** density and water permeability,were related to wood decomposition rate and its pattern over *** half-time related positively and negatively to initial wood density and water permeability,*** time-dependentrate model fitted better for 18 species(56%of the study species)that had higher water permeabilities than the others,suggesting that micelle porosity in wood relates to temporal changes in decomposition rate.

关键词： Sarawak carbon dynamics fine wood litter wood density tropical rain forest

Ultra-Short Pulse Tracking by Using Wavelength Dispersion for a Short-Time Optical Buffer

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光学学报 2003年第S1期23卷 601-602页

作者： Tsuyoshi Konishi Hideaki Furukawa Kousuke Asano Kazuyoshi itoh Graduate School of Engineering Department of Material and Life Science Osaka University JAPANGraduate School of Engineering Department of Material and Life Science Osaka University JAPANGraduate School of Engineering Department of Material and Life Science Osaka University JAPANGraduate School of Engineering Department of Material and Life Science Osaka University JAPAN

To synchronize a control signal with a packet signal in response to changing timing jitter, we investigate ultra-short pulse tracking by using wavelength dispersion for a short-time optical buffer in an optical router.

关键词： for on in by Ultra-Short Pulse Tracking by Using Wavelength Dispersion for a Short-Time Optical Buffer

Identification of two metallo- thionein isoforms by molecular cloning of their cDNAs in fresh-water fish,crucian carp (Carassius-cuvieri)

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Chinese Science Bulletin 2001年第1期46卷 53-57页

作者： REN Hongwei Muto Norio itoh Norio YU Meimin RU Binggeng Tanaka Keiichi National Laboratory of Protein Engineering College of Life SciencesPeking UniversityBeijing 100871China Department of Environmental Toxicology Faculty of Pharmaceutical SciencesOsaka University1-6 YamadaokaSuitaOsaka 565-0871Japan

Generally, there are two major isoforms of me-tallothionein (MT) in mammals. In this study two cDNAs of metallothionein, MT-A and MT-B, in a fresh-water fish, crucian carp (Carassius cuvieri), were cloned by the rapid amplification of cDNA ends (RACE). The homology of their reading frame is about 92.3%. The sequence analysis of both cDNAs gave the structures of coding regions corresponding to 60 amino acid residues, and the structures of complete 3’ -untranslated regions in which a significant difference in the size of their 3’-untranslated regions (130 bp for MT-A and 280 bp for MT-B) exists. The results of amino acid sequencing of both MT-1 and MT-2 purified by HPLC are identical to those deduced from MT cDNA genes, indicating that MT-1 is from MT-A gene and MT-2 is from MT-B gene respectively. No blocking in the N-terminal of MT-2 isoform was the first case found in vertebrates, most of which were blocked by acetylation. These results suggest that there were differential controls at the

关键词： metallothionein isoform carassius-cuvieri suppression RACE.

Risk factors of gastric cancer specific for tumor location and histology in Cali,Colombia

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World Journal of Gastroenterology 2006年第36期12卷 5772-5779页

作者： Francia Campos Gabriel Carrasquilla Chihaya Koriyama Mauricio Serra Edwin Carrascal Tetsuhiko itoh Mitsuharu Nomoto Suminori Akiba Depart-ment of Epidemiology and Preventive Medicine Kagoshima Uni-versity Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima 890-8544 Japan Department of Microbiology Faculty of Health Sciences Universidad del Valle San Fernando Cali Co-lombia Health Division FES Foundation Cali Colom-bia Department of Pathology Faculty of Health Sciences Universidad del Valle San Fernando Cali Colombia Kaisei-en Geriatric Health Services Facility 551 Akasegawa Akune Kagoshima Japan Department of Human Pathology Kagoshi-ma University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima 890-8544 Japan

AIM- To examine histology- and tumor-location specific risk factors of gastric cancer （GC）.METHODS： This was subjects were 216 GC the period 2000-2002 non-cancer patients hospital. We obtained habits, and others by a a case-control study. The study patients newly diagnosed during and 431 controls selected from matching in age, gender, and information on lifestyles, dietary ***： The subjects who were not eldest among his/her siblings were at a slightly elevated GC risk （OR 1.3; 95% CI 0.8-2.0）. Salting meals before tasting was related to an increased GC risk （OR 3.5; 95% CI 1.6- 7.3）. Frequent consumptions of fruits （OR 0.3; 95% CI 0.1-1.0） and vegetables （OR 0.3; 95% CI 0.1-1.0） were related to decreased GC risks. On the other hand, frying foods （OR 1.9; 95% CI 1.0-3.6） and cooking with coal （OR 1.8; 95% CI 1.3-2.6） were related to increased GC risks. Neither Lauren＇s histological classification （intestinal and diffuse types） nor tumor location significantly affected those associations except birth order. The subjects who were not eldest among his/her siblings had an increased risk of GCs in the distal and middle thirds, and their ORs were 1.7 （95% CI 1.0-2.8） and 1.9 （95% CI 0.8-4.3）, respectively. The corresponding OR in the upper third stomach was 0.3 （95% CI 0.1-0.9）. The differences of those three ORs were statistically significant （P = 0.010）.CONCLUSION： The present study shows that birth order, salt intake, consumption of fruits and vegetables, the type of cooking, and cigarette smoking are related to GC risk. In histology and tumor-location specific analyses, non-eldest person among their siblings is related to an increased GC risk in the distal and middle thirds of the stomach, and is related to a decreased GC risk in the cardia.

关键词： Gastric cancer Risk factor Tumor location Histological type Colombia

E-cadherin and beta-catenin expression in Epstein-Barr virus-associated gastric carcinoma and their prognostic significance

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World Journal of Gastroenterology 2007年第29期13卷 3925-3931页

作者： Chihaya Koriyama Suminori Akiba Tetsuhiko itoh Kazunobu Sueyoshi Yoshie Minakami Alejandro Corvalan Suguru Yonezawa Yoshito Eizuru Department of Epidemiology and Preventive Medicine Kagoshima University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima Japan Geriatric Health Services Facility 551 Akasegawa Akune Kagoshima Japan Kazunobu Sueyoshi Department of Pathology Kagoshima City Hospital 20-17 Kajiya-cho Kagoshima Japan Division of Oncogenic and Persistent Viruses Center for Chronic Viral Diseases Kagoshima University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima Japan Department of Anatomic Pathology P. Catholic University Santiago Chile Department of Human Pathology Kagoshima University Graduate School of Medical and Dental Sciences 8-35-1 Sakuragaoka Kagoshima Japan

AIM： To examine the role of E-cadherin and betacatenin in carcinogenesis and to assess their prognostic implication in Epstein-Barr virus-associated gastric carcinomas （EBV-GCs）. METHODS： We compared the frequency of E-cadherin and beta-catenin expression in 59 EBV-GCs and 120 non-EBV-GCs, and examined the association between patients＇ prognosis and the expressions of these proteins. RESULTS： Neither the cellular-membranous nor the cytoplasmic E-cadherin expression showed any difference between EBV-GCs and non-EBV-GCs. On the other hand, loss of membranous expression of beta- catenin occurred more frequently in non-EBV-GCs than EBV-GCs [odds ratio = 0.41; 950 confidence interval （CI）, 0.19-0.90]. Furthermore, the nuclear and/or cytoplosmic expression of beta-catenin was seen more frequently in EBV-GCs than non-EBV-GCs （odds ratio = 2.23; 95% CI, 0.97-5.09）, and was observed in a larger proportion of carcinoma cells of EBV-GCs than non-EBV-GCs （P = 0.024）. Survival analysis for non-EBV-GC revealed that lymph node metastasis was significantly associated with poor prognosis （P 〈 0.001）. Among EBV- GCs, the depth of invasion （P = 0.005）, lymph node metastasis （P = 0.004） and an intestinal type by Lauren classification （hazard ratio = 9.47; 95% CI, 2.67-33.6） were significantly associated with poor prognosis. On the other hand, nuclear and/or cytoplasmic expression of beta-catenin was associated with a better prognosis in patients with EBV-GC （hazard ratio = 0.32; 95% CI, 0.11-0.93）. CONCLUSION： We observed more frequent preservation of beta-catenin in cell membrane and accumulation in nuclei and/or cytoplasm in EBV-GCs than in non-EBV- GCs. Factors involved in the prognosis of EBV-GCs and non-EBV-GCs are different in the two conditions.

关键词： Epstein-Barr virus Gastric carcinoma E-cadherin Beta-catenin Prognosis

Need for pancreatic stenting after sphincterotomy in patients with difficult cannulation

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World Journal of Gastroenterology 2014年第26期20卷 8617-8623页

作者： Kazunari Nakahara Chiaki Okuse Keigo Suetani Yosuke Michikawa Shinjiro Kobayashi Takehito Otsubo Fumio itoh Department of Gastroenterology and Hepatology St. Marianna University School of Medicine Department of Gastroenterological and General Surgery St. Marianna University School of Medicine

AIM: To investigate the need for pancreatic stenting after endoscopic sphincterotomy (EST) in patients with difficult biliary cannulation.

关键词： Pancreatic stenting Pancreatic guidewire placement Post-endoscopic retrograde cholangiopancreatography pancreatitis Endoscopic sphincterotomy Endoscopic retrograde cholangiopancreatography

Jumbo biopsy is useful for the diagnosis of colonic prolapsing mucosal polyps with diverticulosis

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World Journal of Gastroenterology 2006年第10期12卷 1634-1636页

作者： Shingo Kato Kazutoshi Hashiguchi Ryuichi Yamamoto Mitsuru Seo Takashi Matsuura Kazuro itoh Akinori Iwashita Soichiro Miura Fukuoka Japan Self Defense Forces Hospital Japan Second Department of Internal Medicine National Defense Medical CollegeTokorozawa CityJapan Second Department of Internal Medicine National Defense Medical College Tokorozawa CityJapan KKR Hamanomachi Hospital Department of Pathology Chikushi Hospital Fukuoka UniversityJapan

We report here a case of multiple prolapsing mucosal polyps with diverticulosis in the sigmoid colon. A 52-year- old man was admitted to our hospital because of bloody diarrhea. Colonoscopy and barium enema showed multiple diverticula, markedly thickened mucosal folds and polypoid lesions with mucus on the top of them in the sigmoid colon. Endoscopic ultrasonography showed thickening of the mucosal and submucosal layers. Several endoscopic biopsy specimens were taken from the polypoid lesions. Histological examination revealed only chronic inflammatory cell infiltration. In order to obtain a definite diagnosis, we performed endoscopic jumbo biopsy for the polypoid lesions after obtaining informed consent. Histological examination revealed marked lymphocyte infiltration, hemosiderin deposits and fibromuscular obliteration in the lamina propria, features similar to those of mucosal prolapsing syndrome. After anti-diarrhetic treatment, clinical findings were improved. Thus, jumbo biopsy is useful for diagnosis and treatment of prolapsing mucosal polyps.

关键词： Colon Diverticulum Jumbo biopsy Prolapsing mucosal polyp

Crosstalk Suppression in Header Recognition Unit Using Time-Space Conversion

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光学学报 2003年第S1期23卷 633-634页

作者： Hideaki Furukawa Tsuyoshi Konishi Yoshinori Oshita Kazuyoshi itoh Yoshiki Ichioka Department of Material and Life Science Osaka University Japan.Department of Material and Life Science Osaka University Japan.Department of Material and Life Science Osaka University Japan.Department of Material and Life Science Osaka University Japan.Department of Material and Life Science Osaka University Japan.

We demonstrate a suppression effect of a crosstalk among a target and similar header signals using the header recognition filter designed by a design technique of the multiple-object discriminant filter.

关键词： in it et of that with As be