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Science China Chemistry 2012年第5期55卷 850-853页

作者： TAGAYA Akihiro LOU LiPing ide yoko KOIKE Yasuhiro OKAMOTO Yoshiyuki Keio Photonics Research Institute Keio University E-building Shin-Kawasaki Town Campus 7-1 Shin-Kawasaki Saiwai-ku Kawasaki 212-0032 Japan Faculty of Science and Technology Keio University 3-14-1 Hiyoshi Kohoku-ku Yokohama 223-8522 Japan Polymer Research Institute Polytechnic Institute of NYU 6 MetroTech Center Brooklyn New York 11201 USA

Copolymers of N-pentafluorophenyl maleimide (PFPMI) with methyl methacrylate (MMA) were synthesized by a free radical initiator, such as AIBN. The refractive indexes of the copolymers remained nearly constant (1.4970 at 532 nm) regardless of the polymer composition. These copolymers also showed high thermal stability. The orientational and photoelastic birefringence of the copolymers obtained were measured. Since both of the orientational and photoelastic birefringences of PMMA are negative whereas poly(PFPMI) exhibits positive, thus we have obtained nearly zero orientational and photoelastic birefringence polymers when the ratios of MMA/PFPMI were 91.8/8.2 and 97.0/3.0 mol%, respectively. Based on the experimental data, the ratios of MMA/PFPMI for zero birefringence were determined to be 88.9/11.1 and 93.8/6.2 mol% for orientational and photoelastic birefringence, respectively. The Tgs of corresponding copolymers were estimated to be 128 and 122 ℃.

关键词： N-pentafluorophenyl maleimide copolymers orientational birefringence photoelastic birefringence

靶向白细胞介素1β纳米抗体的制备及其对缺氧小鼠心肌细胞凋亡的影响

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中华医学杂志 2024年第22期104卷 2066-2073页

作者：欧阳情王露 Nasser Moussa ide 邓刚张新奎游婷苏浩滔朱平华南理工大学医学院广州广东省心血管病研究所南方医科大学附属广东省人民医院(广东省医学科学院)广东省心脏病发病机制与精准防治重点实验室广州市心脏发病机制与重点防治重点实验室广州

目的制备抗白细胞介素1β(IL-1β)纳米抗体, 并观察其对缺氧小鼠心肌细胞凋亡的影响。方法利用DNA重组技术, 使用pET-16b和pHEN1表达载体构建抗IL-1β纳米抗体的重组质粒(pET-16b-4G6M-VHH、pET-16b-5BVP-VHH、pET-16b-5MVZ-VHH和pHEN1-... 详细信息

目的制备抗白细胞介素1β(IL-1β)纳米抗体, 并观察其对缺氧小鼠心肌细胞凋亡的影响。方法利用DNA重组技术, 使用pET-16b和pHEN1表达载体构建抗IL-1β纳米抗体的重组质粒(pET-16b-4G6M-VHH、pET-16b-5BVP-VHH、pET-16b-5MVZ-VHH和pHEN1-4G6M-VHH、pHEN1-5BVP-VHH、pHEN1-5MVZ-VHH, 其中VHH为重链单域抗体, 4G6M-VHH、5BVP-VHH、5MVZ-VHH分别为3种抗IL-1β的纳米抗体), 将构建成功的质粒分别转入大肠杆菌Rosetta2(DE3)表达菌中进行诱导表达及镍柱纯化, 用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western印迹对表达产物及纯化产物进行鉴定, 并通过酶联免疫吸附试验(ELISA)测定其亲和力。将纯化所得亲和力最高的抗IL-1β纳米抗体(pHEN1-5MVZ-VHH)应用于小鼠HL-1心肌细胞缺氧模型(分为正常对照组、缺氧模型组、空白质粒组及12.5、25.0、50.0 μg/ml pHEN1-5MVZ-VHH处理组), 并检测细胞存活率及凋亡率。结果 SDS-PAGE和Western印迹结果显示, 成功得到相对分子质量约15 000的抗IL-1β纳米抗体;ELISA结果显示, 与pET-16b载体组相比, pHEN1载体组所表达的纳米抗体与IL-1β抗原的亲和力更高(4G6M-VHH组:3.20±0.03比1.20±0.03, P<0.001;5BVP-VHH组:3.18±0.06比1.21±0.02, P<0.001;5MVZ-VHH组:3.38±0.05比1.62±0.04, P<0.001);细胞存活及凋亡检测结果显示, 与缺氧模型组相比, 25.0 μg/ml及50.0 μg/ml pHEN1-5MVZ-VHH处理组HL-1细胞活性增加[(75.55±2.23)%比(46.90±2.51)%, P<0.001;(74.36±1.96)%比(46.90±2.51)%, P<0.001], 凋亡率降低[(6.83±0.27)%比(10.24±0.76)%, P<0.001;(6.68±0.38)%比(10.24±0.76)%, P<0.001]。结论 pET-16b和pHEN1载体均能表达出抗IL-1β纳米抗体, 且pHEN1载体表达的纳米抗体具有更好的抗原亲和作用。此外, pHEN1-5MVZ-VHH治疗可减少缺氧小鼠心肌细胞凋亡。

关键词：缺氧心肌细胞细胞凋亡白细胞介素1β 纳米抗体原核表达

Genetic relationships among sympatric varieties of Acer mono in the Chichibu Mountains and Central Hokkaido, Japan

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Journal of Forestry Research 2017年第4期28卷 699-704页

作者： Chunping Liu Jian Cong Hailong Shen Cunxue Lin yoko Saito Yuji ide State Key Laboratory of Forest Genetics and Breeding Northeast Forestry UniversityHarbin 150040China Foreign Capital Project Office Forestry Department of Liaoning ProvinceShenyang 110036China Mudanjiang Branch of Heilongjiang Academy of Agricultural Sciences Mudianjiang 157041China Laboratory of Forest Ecosystem Studies Department of Ecosystem StudiesGraduate School of Agriculture and Life ScienceThe University of TokyoYayoi 1-1-1Bunkyo-kuTokyo 113-8657Japan

Acer mono Maxim. is one of the major components of cool temperate forests in Japan. Some of its many varieties are distributed sympatrically. Because of its great variability, the intraspecific taxonomy and nomenclature of the species are controversial. To understand the genetic relationships among these varieties and whether hybridization or introgression occurred among the sympatric varieties, we studied the genetic relationships among sympatric varieties of A. mono in the Chichibu Mountains (A. mono var. ambiguum, A. mono var. connivens, A. mono var. marmoratum) and Central Hokkaido (A. mono var. mayrii and A. mono var. glabrum) in Japan. Our results showed that varieties in Chichibu are genetically close, suggesting that hybridization or introgression might occur between these varieties, which could explain the higher genetic diversity of varieties in Chichibu than in Hokkaido. In contrast to the close relationships between the varieties in Chichibu, varieties in Hokkaido seemed relatively separated from each other; indeed, there may be reproductive isolation between the two varieties. The results provide new insight for the taxonomy of the varieties of A. mono, especially the sympatric varieties, in Japan.

关键词： Genetic differentiation Hybridization Introgression Maple nSSR

Long Pulse Operation of High Performance Plasmas in JT-60U

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Plasma Science and Technology 2006年第1期8卷 1-4页

作者： S.ide the 3T-60 Team Naka Fusion Research Establishment Japan Atomic Energy Agency NakaIbaraki311-0193Japan

Recent experimental progress in JT-60U advanced tokamak research is presented： sustainment of the normalized beta （βN）- 3 in a normal magnetic shear plasma, the bootstrap current fraction （fBs） - 45% in a weak shear plasma and - 75% in a reversed magnetic shear plasma in a nearly fully non-inductive current drive condition for longer than the current relaxation time. Achievement of high-density, high-radiation fraction together with high-confinement in advanced plasmas is demonstrated. Achievements and findings in long pulse operations after system modification are presented as well. A 65 s discharge of Ip = 0.7 MA was successfully obtained. As a result, high-βN of 2.3 was successfully sustained for a very long period of 22.3 s. In addition, a 30 s standard ELMy H-mode plasma of Ip up to 1.4 MA was also obtained. Effectiveness of divertor pumping to control particle recycling and the electron density under the saturated wall retention was demonstrated. These achievements and issues in development are discussed.

关键词： transport properties magnetohydrodynamics tokamaks

Bred Vectors of the Lorenz63 System

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Advances in Atmospheric Sciences 2015年第11期32卷 1533-1538页

作者： Ying ZHANG Kayo ide Eugenia KALNAY Department of Atmospheric and Oceanic Science University of MarylandCollege Park

The breeding method has been widely used in studies of data assimilation, predictability and instabilities. The bred vectors （BVs）, which are the nonlinear difference between the control and perturbed runs, represent the time-evolving rapidly growing errors in dynamic systems. The Lorenz （1963） model （hereafter Lorenz63 model） has chaotic dynamics similar to weather and climate. This study investigates the features of BVs of the Lorenz63 model and its impact on regime prediction of the Lorenz63 model. The results show that the Lorenz63 model has two different BVs for each breeding cycle, and the two BVs approach being identical when growth rate is high. The duration of the current and next regime is associated with the relative directions between the BV with high growth rate and the model trajectory.

关键词： bred vectors chaos Lorenz63 model regime prediction

轻度认知功能障碍病人MRI显示脉络丛增大的大型队列研究

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国际医学放射学杂志 2024年第5期 625-626页

作者： Y.Umemura K.Watanabe S.Kasai S.ide Y.Ishimoto M.Sasaki 陈瑾

目的以往研究表明阿尔茨海默病（AD）可能存在脉络丛（CP）功能障碍，并特别提出MRI所见的CP增大可作为AD预测指标。但探讨CP体积（CPV）与轻度认知障碍

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Enzyme-digested Colla Corii Asini(E’jiao) prevents hydrogen peroxide-induced cell death and accelerates amyloid beta clearance in neuronal-like PC12 cells

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Neural Regeneration Research 2020年第12期15卷 2270-2277页

作者： Li Xiao Feng Liao Ryoji ide Tetsuro Horie Yumei Fan Chikako Saiki Nobuhiko Miwa Department of Pharmacology The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan National Engineering Research Center for Gelatin-based Traditional Chinese Medicine Dong-E-E-Jiao Co.Ltd.LiaochengShandong ProvinceChina Department of Physiology The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan Research Center The Nippon Dental UniversitySchool of Life Dentistry at TokyoTokyoJapan Department of Life Sciences Prefectural University of HiroshimaHiroshimaJapan

As an aging-associated degenerative disease,Alzheimer’s disease is characterized by the deposition of amyloid beta(Aβ),oxidative stress,inflammation,dysfunction and loss of cholinergic *** Corii Asini(CCA)is a traditional Chinese medicine which has been used for feebleness-related diseases and *** might delay aging-induced degenerative changes in *** the present study,we evaluated antioxidant activity,cytoprotective effects,and Aβremovability of enzyme-digested Colla Corii Asini(CCAD).Oxygen radical absorbance capacity(ORAC)activity assay showed that,as compared to gelatins from the skin of porcine,bovine and cold water fish,CCA exhibited the highest ORAC *** ORAC activity of CCA and CCAD was increased gradually by the length of time in *** analysis by scanning electron microscopy showed that among CCA manufactured in 2008,2013,2017 and gelatin from cold water fish skin,CCA manufactured in 2008 presented the smoothest surface *** further tested the protective effects of CCAD(manufactured in 2008)and enzyme-digested gelatin from cold water fish skin(FGD)on hydrogen peroxide(H2O2)-induced cell death in nerve growth factor-differentiated neuronal-like PC12 *** blue assay showed that both FGD and CCAD at 0.5 mg/m L increased cell viability in H2O2-treated neuronal-like PC12 *** protection of CCAD was significantly superior to that of ***(Ach E)assay showed that both FGD and CCAD inhibited Ach E activity in nerve growth factor-differentiated neuronal-like PC12 cells to 89.1%and 74.5%of that in non-treated cells,*** data suggest that CCAD might be able to increase the neurotransmitter *** CCAD inhibited Ach E activity in neuronal-like PC12 cells,CCAD prevented H2O2-induced abnormal deterioration of Ach *** and neprilysin activity assay results indicated that CCAD reduced amyloid beta accumulation and increased neprilysin activity in Aβ1–

关键词： acetylcholinesterase activity Alzheimer’s disease amyloid beta clearance antioxidant Colla Corii Asini collagen neuroprotection PC12 cells

Points regarding cell transplantation for the treatment of spinal cord injury

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Neural Regeneration Research 2016年第7期11卷 1046-1049页

作者： Chizuka ide Kenji Kanekiyo Institute of Regeneration and Rehabilitation Aino University School of Health Science

Transplantation of somatic cells,including bone marrow stromal cells（BMSCs）,bone marrow mononuclear cells（BMNCs）,and choroid plexus epithelial cells（CPECs）,enhances the outgrowth of regenerating axons and promotes locomotor *** are not integrated into the host spinal cord,but disappear within 2-3 weeks after *** axons extend at the spinal cord lesion through the astrocyte-devoid area that is filled with connective tissue *** axons have characteristics of peripheral nerves：they are associated with Schwann cells,and embedded in connective tissue *** has been suggested that neurotrophic factors secreted from BMSCs and CPECs promote “intrinsic” ability of the spinal cord to *** Schwann cells survive long-term,and are integrated into the host spinal cord,serving as an effective scaffold for the outgrowth of regenerating axons in the spinal *** disadvantage that axons are blocked to extend through the glial scar at the border of the lesion is *** cells have been approved for clinical *** stem/progenitor cells（NSPCs） survive long-term,proliferate,and differentiate into glial cells and/or neurons after *** method is available at present to manipulate and control the behaviors of NPSCs to allow them to appropriately integrate into the host spinal *** transplantation is not necessarily effective for locomotor improvement.

关键词： Schwann progenitor glial astrocyte neurotrophic differentiate scaffold connective survive proliferate

Impact of smoking habit on surgical outcomes in non-B non-C patients with curative resection for hepatocellular carcinoma

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World Journal of Gastroenterology 2017年第8期23卷 1397-1405页

作者： Keita Kai Hiroki Koga Shinichi Aishima Atsushi Kawaguchi Koutaro Yamaji Takao ide Junji Ueda Hirokazu Noshiro Department of Pathology Saga University Hospital Departments of Surgery Saga University Hospital Pathology and Microbiology Saga University Hospital Center for Comprehensive Community Medicine Saga University Faculty of Medicine

To analyzed the correlation between smoking status and surgical outcomes in patients with non-B non-C hepatocellular carcinoma (NBNC-HCC), and we investigated the patients’ clinicopathological characteristics according to smoking *** retrospectively analyzed the consecutive cases of 83 NBNC-HCC patients who underwent curative surgical treatment for the primary lesion at Saga University Hospital between 1984 and December 2012. We collected information about possibly carcinogenic factors such as alcohol abuse, diabetes mellitus, obesity and smoking habit from medical records. Smoking habits were subcategorized as never, ex- and current smoker at the time of surgery. The diagnosis of non-alcoholic steatohepatitis (NASH) was based on both clinical information and pathological *** abuse, diabetes mellitus, obesity and NASH had no significant effect on the surgical outcomes. Current smoking status was strongly correlated with both overall survival (P = 0.0058) and disease-specific survival (P = 0.0105) by multivariate analyses. Subset analyses revealed that the current smokers were significantly younger at the time of surgery (P = 0.0002) and more likely to abuse alcohol (P = 0.0188) and to have multiple tumors (P = 0.023).CONCLUSIONCurrent smoking habit at the time of surgical treatment is a risk factor for poor long-term survival in NBNC-HCC patients. Current smokers tend to have multiple HCCs at a younger age than other patients.

关键词： Hepatocellular carcinoma Non-B Non-C Smoking Surgery Prognosis

Double Filtration Plasmapheresis and (PEG) Interferon/Ribavirin Combination Therapy in Patients with Chronic Hepatitis C (Abstract)

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Double Filtration Plasmapheresis and (PEG) Interferon/Ribavi...