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Allotopic expression of mitochondrial genes:Basic strategy and progress

Genes & Diseases 2020年第4期7卷 578-584页

作者： i.made artika Department of Biochemistry Faculty of Mathematics and Natural SciencesBogor Agricultural UniversityDarmaga CampusBogor 16680Indonesia Eijkman institute for Molecular Biology Jalan Diponegoro 69Jakarta10430Indonesia

Allotopic expression of mitochondrial genes is a deliberate functional relocation of mitochondrial genes into the nucleus followed by import of the gene-encoded polypeptide from the cytoplasm into the *** successful allotopic expression of a mitochondrial gene,several key aspects must be *** include the different codon dictionary used by the mitochondrial and nuclear genomes,different codon preferences between mitochondrial and nuclear-cytosolic translation systems,and the provision of an import signal to ensure that the newly translated protein in the cytosol is successfully imported into *** allotopic expression strategy was first developed in yeast,a useful model organism for studying human and other eukaryotic ***,a number of mitochondrial genes have been successfully recoded and nuclearly expressed in yeast and human *** addition to its use in evolutionary and molecular biology studies,the allotopic expression strategy has been developed as a potential approach to treat mitochondrial genetic *** progress has been recently achieved,and the development of this technique for therapy of the mitochondrial disease Leber’s hereditary optic neuropathy(LHON)has entered phase iii clinical ***,a number of challenges remain to be overcome to accelerate the successful application of this *** include improvement of nuclear gene expression,import into mitochondria,processing,and functional integration of the allotopically expressed polypeptides into mitochondrial protein *** review discusses the current basic strategy,progress,challenges,and prospects of the allotopic expression strategy for mitochondrial genes.

关键词： Allotopic expression Gene therapy Mitochondria Mitochondrial gene Protein targeting

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Laboratory biosafety for handling emerging viruses

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Asian Pacific Journal of Tropical Biomedicine 2017年第5期7卷 483-491页

作者： i.made artika Chairin Nisa Ma’roef Biosafety Level 3 Unit Eijkman Institute for Molecular Biology Department of Biochemistry Faculty of Mathematics and Natural SciencesBogor Agricultural UniversityDarmaga Campus Emerging Virus Research Unit Eijkman Institute for Molecular Biology

Emerging viruses are viruses whose occurrence has risen within the past twenty years,or whose presence is likely to increase in the near *** caused by emerging viruses are a major threat to global public *** spite of greater awareness of safety and containment procedures,the handling of pathogenic viruses remains a likely source of infection,and mortality,among laboratory *** is a steady increase in both the number of laboratories and scientist handling emerging viruses for diagnostics and *** potential for harm associated to work with these infectious agents can be minimized through the application of sound biosafety concepts and *** main factors to the prevention of laboratory-acquired infection are well-trained personnel who are knowledgable and biohazard aware,who are perceptive of the various ways of transmission,and who are professional in safe laboratory practice *** addition,we should emphasize that appropriate facilities,practices and procedures are to be used by the laboratory workers for the handling of emerging viruses in a safe and secure *** review is aimed at providing researchers and laboratory personnel with basic biosafety principles to protect themselves from exposure to emerging viruses while working in the *** paper focuses on what emerging viruses are,why emerging viruses can cause laboratory-acquired infection,how to assess the risk of working with emerging viruses,and how laboratory-acquired infection can be *** measures used in the laboratory designed as such that they protect workers from emerging viruses and safeguard the public through the safe disposal of infectious wastes are also addressed.

关键词： Biosafety Emerging viruses Laboratory acquired infection Biorisk assessment Biorisk management

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Anticancer activity test of ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.)

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Asian Pacific Journal of Tropical Medicine 2017年第6期10卷 635-640页

作者： Minarni i.made artika Heddy Julistiono Nurliani Bermawie Eny ida Riyanti Hasim Akhmad Endang Zainal Hasan Department of Biochemistry Faculty of Mathematics and SciencesBogor Agricultural UniversityDarmaga CampusBogor 16680Indonesia Research Center for Biology The Indonesian Institute of Sciences(LIPI)Cibinong-Bogor Indonesia Research institute of Medicinal and Aromatic Plants Agricultural Research AgencyBogor-Indonesia Center for Biotechnology and Genetic Resources of Agriculture Agricultural Research AgencyBogor-Indonesia

Objective: To analyze anticancer activity of an ethyl acetate extract of endophytic fungi isolated from soursop leaf(Annona muricata L.). Methods: Anticancer activity of fungal extracts was determined by observing its toxicity against MCF-7(Michigan Cancer Foundation-7) cells in vitro by using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide(MTT) assay method. At an extract concentration of 100 μg/m L, 4 isolates out of 12 showed high activity against the cancer cell growth. The four isolates were then selected for further iC50 determination, by measuring the inhibition of cancer cell proliferation at extract concentration of 25 μg/m L, 50 μg/m L, 100 μg/m L, 200 μg/m L and 400 μg/m L. Results: Results showed that isolate Sir-G5 had the highest anticancer activity with an iC50 of 19.20 μg/m L. The best isolates were screened again using a normal cell(Chang cells) to determine its toxicity against normal cells. Results indicated that the extracts do not affect the proliferation of normal cells. Molecular identification showed that the fungal isolate Sir-G5 has a close relationship with Phomopsis sp. Conclusions: The endophytic fungi isolated from soursop leaf has the potential to be used as a source of anticancer agents.

关键词： Annona muricata L Anticancer MCF-7 Endophytic fungi Soursop leaf

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