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Engineering Microbiology 2024年第3期4卷 1-15页

作者： i.c.cunha-ferreira c.S.Vizzotto T.D.Frederico J.Peixoto L.S carvalho M.R.Tótola R.H.Krüger Laboratory of Enzymology Department of Cellular BiologyUniversity of Brasília(UNB)Brasília70910-900Brazil Laboratory of Environmental Sanitation Department of Civil and Environmental EngineeringUniversity of Brasília(UNB)Brasília70910-900Brazil Laboratório de Biotecnologia e Biodiversidade para o Meio Ambiente Universidade Federal de Viçosa(UFV)Viçosa36570-900Brazil

The biotechnological industry faces a crucial demand for novel bioactive compounds,particularly antimicrobial agents,to address the rising challenge of bacterial resistance to current available *** strate-gies for cultivating naturally occurring microorganisms often limit the discovery of novel antimicrobial *** study presents a protocol for targeted selection of bacterial strains using the supernatant of Paenibacillus el-gii,which produces abundant signal molecules and antimicrobial *** samples were inoculated in these enriched culture media to selectively cultivate bacteria resistant to the supernatant,indicating their potential to produce similar *** bacterial strains isolated through this method were assessed for their antibac-terial *** addition,the functional annotation of the genome of one of these strains revealed several gene clusters of biotechnological *** study highlights the effectiveness of using this approach for selective cultivation of microorganisms with potential for biotechnological applications.

关键词： Prospecting Supernatant Antibacterial activity

A facile strategy for tuning the density of surface-grafted biomolecules for melt extrusion-based additive manufacturing applications

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Bio-Design and Manufacturing 2024年第3期7卷 277-291页

作者： i.A.O.Beeren G.Dos Santos P.J.Dijkstra c.Mota J.Bauer H.ferreira Rui L.Reis N.Neves S.camarero-Espinosa M.B.Baker L.Moroni Department of complex Tissue Regeneration MERLN Institute for Technology-Inspired Regenerative MedicineMaastricht University6229 ER MaastrichtThe Netherlands 3B’s Research Group I3Bs–Research Institute on BiomaterialsBiodegradables and BiomimeticsHeadquarters of the European Institute of Excellence on Tissue Engineering and Regenerative MedicineUniversity of MinhoAveParkZona Industrial da Gandra4805-017 BarcoGuimarãesPortugal icVS/3B’s-PT Government Associate Laboratory 4806-909 Braga/GuimarãesPortugal POLYMAT University of the Basque Country UPV/EHU20018 Donostia/San SebastiánSpain iKERBASQUE Basque Foundation for Science48009 BilbaoSpain

Melt extrusion-based additive manufacturing(ME-AM)is a promising technique to fabricate porous scaffolds for tissue engi-neering ***,most synthetic semicrystalline polymers do not possess the intrinsic biological activity required to control cell *** of biomolecules on polymeric surfaces of AM scaffolds enhances the bioactivity of a construct;however,there are limited strategies available to control the surface ***,we report a strategy to tune the surface density of bioactive groups by blending a low molecular weight poly(ε-caprolactone)5k(PcL5k)containing orthogonally reactive azide groups with an unfunctionalized high molecular weight PcL75k at different *** porous three-dimensional(3D)scaf-folds were then fabricated using a high weight percentage(75 wt.%)of the low molecular weight PcL *** a proof-of-concept test,we prepared films of three different mass ratios of low and high molecular weight polymers with a thermopress and reacted with an alkynated fluorescent model compound on the surface,yielding a density of 201-561 pmol/cm^(2).Subsequently,a bone morphogenetic protein 2(BMP-2)-derived peptide was grafted onto the films comprising different blend compositions,and the effect of peptide surface density on the osteogenic differentiation of human mesenchymal stromal cells(hMScs)was *** two weeks of culturing in a basic medium,cells expressed higher levels of BMP receptor ii(BMPRii)on films with the conjugated *** addition,we found that alkaline phosphatase activity was only significantly enhanced on films contain-ing the highest peptide density(i.e.,561 pmol/cm^(2)),indicating the importance of the surface *** together,these results emphasize that the density of surface peptides on cell differentiation must be considered at the cell-material ***,we have presented a viable strategy for ME-AM community that desires to tune the bulk and surface functionality via blending of(m

关键词： Additive manufacturing Blending Surface functionalization Surface density click chemistry Human

changes in calcium accumulation and utilization efficiency and their impact on recycling,immobilization,and export across the oil palm cycle

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Oil crop Science 2024年第3期9卷 143-150页

作者： ismael de Jesus Matos Viégas Luma castro de Souza Eric Victor de Oliveira ferreira Milton Garcia costa Glauco André dos Santos Nogueira Vitor Resende do Nascimento candido ferreira de Oliveira Neto capanema campus Federal Rural University of the Amazon(UFRA)Av.Barao de CapanemaCaixa D'agua5514-6434ParaBrazil center of Agricultural and Environmental Sciences Federal University of Maranhao(UFMA)ChapadinhaBR-222km 04SNBoa Vista65500000MaranhaoBrazilc capitao Poço campus Federal Rural University of the Amazon(UFRA)Tv.Pau AmareloS/n-Vila Nova68650-000Capitao PoçoParaBrazil Department of Soil Science Sao Paulo State University(Unesp)School of Agricultural and Veterinarian SciencesJaboticabalVia de Acesso Prof.Paulo Donato CastelaneCastellane-S/N-Vila Industrial14884-900Sao PauloBrazil institute of Agricultural Sciences(icA) Federal Rural University of the Amazon(UFRA)Estr.Principal da Ufra2150Curio-Utinga66077-580BelemParaBrazil

Effective calcium(ca)management is crucial for optimizing oil palm cultivation and enhancing crop *** study aimed to gain insights into the dynamics of ca concentration,accumulation,exportation,immobilization,and recycling in various oil palm organs relative to plant *** experiment was conducted at the Agropalma enterprise site in the northeastern region of Para State,Brazil,evaluating seven plant age treatments:2,3,4,5,6,7,and 8 years *** a completely randomized design with four *** results demonstrated an age-related increase in ca concentration in petioles,rachis,arrows,male inflorescences,peduncles,and ***,ca accumulation exhibited an upward trend in all organs with progressing plant ***,the study revealed an enhanced ca use efficiency across all plant organs in correlation with the age of oil palm *** findings underscore the dynamic nutritional demands of oil palm,influencing ca immobilization,cycling,and export throughout its developmental stages.

关键词： Elaeis guineensis Jacq ca cycling ca export ca immobilization ca use efficiency Plant nutrition Amazon

Effect of biochar on the metabolome of soybean seedlings

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Oil crop Science 2024年第1期9卷 60-68页

作者： Nathalia E.Silva Mariana G.Aguilar Osania E.ferreira Gleicia M.Paulino Jaqueline c.L.carvalho Lúcia P.S.Pimenta Alan R.T.Machado Programa de Pos-graduaçao em ciencias Ambientais Universidade do Estado de Minas GeraisFrutalMinas Gerais38202-436Brazil Departamento de Química Universidade Federal de Minas GeraisMinas GeraisBelo Horizonte31270-901Brazil Departamento de Engenharia Aplicada e Tecnologias Ambientais Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil Departamento de ciencias Exatas Universidade do Estado de Minas GeraisJoao MonlevadeMinas Gerais35930-314Brazil

The use of biochar can have several effects on plant germination,depending on raw material,preparation method and application ***,the molecular mechanisms that lead to those results have yet to be *** aim of this research was to improve the understanding of these mechanisms by characterizing the metabolic effects of sugarcane bagasse biochar on soybean *** types of biochars were prepared by pyrolysis at 300℃(ScB300),400℃(ScB400)and 600℃(ScB600).Then,each one was mixed into sand at 1%,3%,5%(w/w)dose,*** experiment was performed in 8 days of incubation,when the number of germinated seeds and the average radicle length were *** evaluate the metabolome,the dry biomass(DB)was subjected to extraction with a mixture of methanol-d4 and D2O(1:1 v/v).The extracts were submitted to metabolomics analysis by Proton Nuclear Magnetic *** Relative Germination,Relative Average Radicle Growth and Germination index increased in all treatments compared to *** the other hand,the DB increased in all treatments,except for ScB300,at doses of 1%and 3%w/*** metabolites(alanine,asparagine,acetic acid,citric acid,glycerol,fatty acids and sucrose)were identified and quantified in DB extracts as the most influential finding for the separation of *** together,these results strongly suggested that biochars accelerated the catabolism of triacylglycerols to sucrose and induced a slight osmotic stress.

关键词： Germination Metabolomics 1H NMR Pyrolysis Sugarcane bagasse

Effect of operational parameters on the performance of an anaerobic sequencing batch reactor(AnSBR)treating protein-rich wastewater

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Environmental Science and Ecotechnology 2024年第1期17卷 89-99页

作者： Zhe Deng Julian Muñoz Sierra Ana Lucia Morgado ferreira Daniel cerqueda-Garcia Henri Spanjers Jules B.van Lier Delft University of Technology Faculty of Civil Engineering and GeosciencesStevinweg 12628 CNDelftthe Netherlands Veolia Water Technologies Techno center Netherlands B.V.-Biothane Tanthofdreef 212623 EWDelftthe Netherlands KWR Water Research institute Groningenhaven 7P.O.Box 10723430 BBNieuwegeinthe Netherlands institute of Ecology.A.c Cluster Cientifico y Tecnologico BioMimic®Carretera Antigua a Coatepec 351El Haya91073XalapaVeracruzMexico

Treating protein-rich wastewater using cost-effective and simple-structured single-stage reactors presents several *** this study,we applied an anaerobic sequencing batch reactor(AnSBR)to treat protein-rich wastewater from a *** focused on identifying the key factors influencing the removal of chemical oxygen demand(cOD)and the settling performance of the *** AnSBR achieved a maximum total cOD removal of 90%,a protein degradation efficiency exceeding 80%,and a cOD to methane conversion efficiency of over 70%at organic loading rates of up to 6.2 g cOD L^(-1)d^(-1).We found that the variations in both the organic loading rate within the reactor and the hydraulic retention time in the buffer tank had a significant effect on cOD *** hydraulic retention time in the buffer tank and the reactor,which determined the ammonification efficiencies and the residual carbohydrate concentrations in the reactor liquid,affected the sludge ***,the genus clostridium sensu stricto 1,known as protein-and lipids-degraders,was predominant in the *** analysis showed a significant correlation between the core microbiome and ammonification efficiency,highlighting the importance of protein degradation as the governing process in the *** results will provide valuable insights to optimise the design and operation of AnSBR for efficient treatment of protein-rich wastewater.

关键词： Anaerobic digestion Anaerobic sequencing batch reactor (AnSBR) Protein Microbial community Slaughterhouse wastewater

TRANSiENT ANALYSiS OF cOMPOSiTE PLATES BY A LOcAL RADiAL BASiS FUNcTiONS-FiNiTE DiFFERENcE TEcHiQUE

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Acta Mechanica Solida Sinica 2012年第1期25卷 22-36页

作者： c.M.c.Roque D.cunha A.J.M.ferreira iNEGi Faculdade de Engenharia da Universidade do PortoRua Dr.Roberto Frias Departamento de Engenharia Mecnica Faculdade de Engenharia da Universidade do PortoRua Dr.Roberto Frias

The use of local numerical schemes,such as finite differences produces much better conditioned matrices than global collocation radial basis functions ***,finite differ-ence schemes are limited to special *** scattered points,a combination of finite differences and radial basis functions would be a possible *** this paper,we use a higher-order shear deformation plate theory and a radial basis function-finite difference technique for predicting the transient behavior of thin and thick composite *** numerical experiments on beams and composite plates,the accuracy and efficiency of this collocation technique is demonstrated.

关键词： meshless beam composite plate

A system-on-chip microwave photonic processor solves dynamic RF interference in real time with picosecond latency

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Light(Science & Applications) 2024年第1期13卷 91-102页

作者： Weipeng Zhang Joshua c.Lederman Thomas ferreira de Lima Jiawei Zhang Simon Bilodeau Leila Hudson Alexander Tait Bhavin J.Shastri Paul R.Prucnal Department of Electrical and computer Engineering Princeton UniversityPrinceton08544NJUSA NEc Laboratories America Princeton08540NJUSA Department of Electrical and computer Engineering Queen’s UniversityKingstonK7L 3N6OntarioCanada Department of Physics Engineering Physics and AstronomyQueen’s UniversityKingstonK7L 3N6OntarioCanada

Radio-frequency interference is a growing concern as wireless technology advances,with potentially life-threatening consequences like interference between radar altimeters and 5 G cellular *** transceivers mix signals with varying ratios over time,posing challenges for conventional digital signal processing(DSP)due to its high *** challenges will worsen as future wireless technologies adopt higher carrier frequencies and data ***,conventional DSPs,already on the brink of their clock frequency limit,are expected to offer only marginal speed *** paper introduces a photonic processor to address dynamic interference through blind source separation(BSS).Our system-on-chip processor employs a fully integrated photonic signal pathway in the analogue domain,enabling rapid demixing of received mixtures and recovering the signal-of-interest in under 15 *** reduction in latency surpasses electronic counterparts by more than three orders of *** complement the photonic processor,electronic peripherals based on field-programmable gate array(FPGA)assess the effectiveness of demixing and continuously update demixing weights at a rate of up to 305 *** compact setup features precise dithering weight control,impedance-controlled circuit board and optical fibre packaging,suitable for handheld and mobile *** experimentally demonstrate the processor’s ability to suppress transmission errors and maintain signal-to-noise ratios in two scenarios,radar altimeters and mobile *** work pioneers the real-time adaptability of integrated silicon photonics,enabling online learning and weight adjustments,and showcasing practical operational applications for photonic processing.

关键词： processor mixing dynamic

Human leukocyte antigen compatibility and incidence of donorspecific antibodies in pediatric liver transplant recipients

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World Journal of Gastroenterology 2024年第33期30卷 3837-3845页

作者： Melina U Melere Flavia H Feier Jorge Neumann Antônio N Kalil Juliana de M Montagner Luiza S Nader carolina S da Silva Marco Aurélio F Junior Gabriela P coral Guilherme P Bobsin cristina T ferreira Department of Hepatology and Liver Transplantation Santa Casa de Porto AlegrePorto Alegre 90050170Rio Grande do SulBrazil Department of Hepato-biliary-pancreatic Surgery and Liver Transplantation Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil Postgraduation Program in Medicine:Hepatology Federal University of Health Sciences of Porto AlegrePorto Alegre 90050-170Rio Grande do SulBrazil Laboratory of Transplantation immunology Irmandade Santa Casa de Misericórdia de Porto AlegrePorto Alegre 90020-090Rio Grande do SulBrazil

BAcKGROUND Antibody-mediated rejection following liver transplantation(LT)has been increasingly recognized,particularly with respect to the emergence of de novo donor-specific antibodies(DSAs)and their impact on graft *** substantial evidence for adult populations exists,research focusing on pediatric LT outcomes remains *** To investigate the prevalence of human leukocyte antigen(HLA)mismatches and DSA and evaluate their association with rejection episodes after pediatric *** A cohort of pediatric LT recipients underwent HLA testing at Santa casa de Porto Alegre,Brazil,between December 2013 and December *** patients who survived for>30 days after LT with at least one DSA analysis were *** classes i and ii and cross-matches were *** presence of de novo DSA(dnDSA)was evaluated at least 3 months after LT using the Luminex®single antigen bead method,with a positive reaction threshold set at 1000 *** episodes were confirmed by liver *** Overall,67 transplanted children were analyzed;61 received grafts from living donors,85%of whom were related to ***-transplant DSA(class i or ii)was detected in 28.3%of patients,and dnDSA was detected in 48.4%.The median time to DSA detection after LT was 19.7[interquartile range(iQR):4.3-35.6]*** rejection occurred in 13 patients at follow-up,with c4d positivity observed in 5/13 Liver *** median time to rejection was 7.8(iQR:5.7-12.8)*** presence of dnDSA was significantly associated with rejection(36%vs 3%,Pction-free survival rates at 12 and 24 months were 76%vs 100%and 58%vs 95%for patients with dnDSA anti-DQ vs those without,*** Our findings highlight the importance of incorporating DSA assessment into pre-and post-transplantation protocols for pediatric LT *** implications may include immunosuppression minimization strategies based on this analysis in pediatric LT recip

关键词： Human leukocyte antigens Donor-specific antibodies Liver transplantation Pediatric Rejection

Further investigation on the fusion of ^(6)Li with ^(209)Bi target at near-barrier energies

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chinese Physics c 2024年第7期48卷 106-117页

作者：张高龙焦振威张广鑫 M.R.cortes 胡世鹏钱建强 D.Mengoni 屈卫卫李聪博郑云张焕乔孙慧斌王楠张春雷 J.J.Valiente-Dobón D.Testov M.Mazzocco A.Gozzelino c.Parascandolo D.Pierroutsakou M.La commara A.Goasduff D.Bazzacco D.R.Napoli F.Galtarossa F.Recchia A.illana S.Bakes i.Zanon S.Aydin G.de Angelis M.Siciliano R.Menegazzo S.M.Lenzi J.L.ferreira J.Rangel Serkan Akkoyun L.F.canto J.Lubian School of Physics Beihang UniversityBeijing 100191China Sino-French institute of Nuclear Engineering and Technology Sun Yat-Sen UniversityZhuhai 519082China instituto de Física Universidade Federal Fluminense24210-340NiteróiRio de JaneiroBrazil institute for Advanced Study in Nuclear Energy&Safety Shenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Research and Manufacture of High Purity Germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China iNFN Sezione di PadovaPadovaItaly Dipartimento di fisica Astronomia dell'Universita di padova PadovaItaly State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China china institute of Atomic Energy Beijing 102413China The Key Laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China iNFN Laboratori Nazionali di LegnaroI-35020 LegnaroItaly iNFN Sezione di NapoliI-80126 NapoliItaly Dipartimento di Farmacia Universitàdi Napoli"Federico II"I-80131 NapoliItaly Department of Natural and Mathematical Sciences Faculty of EngineeringTarsus University33480 MersinTurkiye Physics Division Argonne National LaboratoryLemont-ILUSA Departamento de Matemaética Física e Computaç˜ao Universidade do Estado do Rio de JaneiroFaculdade de Tecnologia27537-000ResendeRio de JaneiroBrazil Department of Physics Sivas Cumhuriyet UniversitySivasTurkey instituto de Física Universidade Federal do Rio de JaneiroCP 6852821941-972Rio de JaneiroBrazil

The complete and incomplete fusion cross sections for ^(6)Li+^(209)Bi were measured using the in-beamγ-ray method around the coulomb *** cross sections of(deuteron captured)incomplete fusion(icF)products were re-quantified experimentally for this reaction *** results reveal that the icF cross section is equivalent to that of complete fusion(cF)above the coulomb barrier and dominant near or below the coulomb barrier.A theoretical calculation based on the continuum discretized coupled channel(cDcc)method was performed for the aforementioned cF and icF cross sections;the result is consistent with the *** universal fusion function(UFF)was also compared with the measured cF cross section for different barrier parameters,demonstrating that the cF suppression factor is significantly influenced by the choice of potential,which can reflect both dynamic and static effects of breakup on the fusion process.

关键词： cross section incomplete fusion suppression factor weakly bound nuclei

Fusion and one-neutron stripping process for ^(6)Li+^(94)Zr system around the coulomb Barrier

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chinese Physics c 2024年第9期48卷 133-141页

作者： Xuedou Su Guangxin Zhang Shipeng Hu Peiwei Wen H.O.Soler Gaolong Zhang Boshuai cai cenxi Yuan J.L.ferreira Zhenwei Jiao Mingli Wang Xiaoyu Wang Zhen Huang Huanqiao Zhang chunlei Zhang Xiaoguang Wu Yun Zheng congbo Li Tianxiao Liand J.Lubian School of Physics Beihang UniversityBeijing 100191China Sino-French institute of Nuclear Engineering and Technology Sun Yat-sen UniversityZhuhai 519082China institute for Advanced Study in Nuclear Energy&Safety Shenzhen UniversityShenzhen 518060China Shenzhen Key Laboratory of Research and Manufacture of High Purity Germanium Materials and Fetectors Shenzhen UniversityShenzhen 518060China china institute of Atomic Energy Beijing 102413China instituto de Física Universidade Federal FluminenseNiterói 24210-340Rio de JaneiroBrazil State Key Laboratory of Radiation Medicine and Protection School of Radiation Medicine and ProtectionSoochow UniversitySuzhou 215123China The Key Laboratory of Beam Technology and Material Modification of Ministry of Education College of Nuclear Science and TechnologyBeijing Normal UniversityBeijing 100875China

The complete and incomplete fusion cross section as well as one-neutron stripping process of ^(6)Li+^(94)Zr system were measured at the energies around the coulomb barrier by onlineγ-ray *** addition to a 30%suppression factor when compared with the measured total fusion process,the complete fusion cross section in ^(6)Li+^(94)Zr system was observed to be significantly lower than those in the nearby ^(6)Li+^(90,96)Zr *** new experimental result implies that the coupling with breakup channel in the ^(6)Li-induced fusion processes can be affected by the inner structure of the target,which is still not clear in any available model *** the one-neutron stripping process,the direct production cross sections for each level in 95Zr were extracted and compared with the coupled reaction channel calculation,offering a unique opportunity to examine the single-particle nature of the produced excited *** the fact that an overall overestimation of the production cross section for 954-keV and1618-keV levels was observed in the comparison,further investigation is highly demanded in order to understand the full reaction mechanism for the one-neutron stripping process induced by ^(6)Li.

关键词： weakly bound nuclei complete fusion incomplete fusion transfer reaction