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Titanium dioxide induced inflammation in the small intestine

World Journal of Gastroenterology 2012年第34期18卷 4729-4735页

作者： Carolina Maciel Nogueira Walter Mendes de Azevedo Maria Lucia Zaidan dagli Sérgio Hiroshi Toma AndréZonetti de Arruda Leite Maria Laura Lordello iêda nishitokukado Carmen Lúcia Ortiz-Agostinho Maria irmaSeixas Duarte Marcelo Alves Ferreira Aytan Miranda Sipahi Department of Gastroenterology Laboratory of Clinical and Experimental Gastroen-terologySchool of MedicineUniversity of So PauloCerqueira César01246-900 So PauloBrazil Department of Fundamental Chemistry Federal University of PernambucoCidade Universitária50670-901 RecifeBrazil Department of Pathology Veterinary Medicine SchoolUniversity of So PauloCidade Universitária05508-270 So PauloBrazil Laboratory of Supramolecular Chemistry and Nanotechnology Chemistry InstituteUniversity of So PauloCidade Universitária05513-970 So PauloBrazil Laboratory of Transmissible Disease Medicine SchoolUniversity of So PauloCerqueira César01246-900 So PauloBrazil Laboratory of Cellular Biology Medicine SchoolUniversity of So PauloCerqueira César01246-900 So PauloBrazil

AiM:To investigate the effects of titanium dioxide (TiO2) nanoparticles (NPTiO 2 ) and microparticles (MPTiO 2 ) on the inflammatory response in the small intestine of mice. METHODS: Bl 57/6 male mice received distilled water suspensions containing TiO 2 (100 mg/kg body weight) as NPTiO 2 (66 nm), or MPTiO 2 (260 nm) by gavage for 10 d, once a day; the control group received only distilled water. At the end of the treatment the duodenum, jejunum and ileum were extracted for assessment of cytokines, inflammatory cells and titanium content. The cytokines interleukin (iL)-1b, iL-4, iL-6, iL-8, iL-10, iL-12, iL-13, iL-17, iL-23, tumor necrosis factor-α (TNF-α), intracellular interferon-γ (iFN-γ) and transforming growth factor-β (TGF-β) were evaluated by enzyme-linked immunosorbent assay in segments of jejunum and ileum (mucosa and underlying muscular tissue). CD4 + and CD8 + T cells, natural killer cells, and dendritic cells were evaluated in duodenum, jejunum and ileum samples fixed in 10% formalin by immuno-histochemistry. The titanium content was determined by inductively coupled plasma atomic emission spectrometry. RESULTS: We found increased levels of T CD4 + cells (cells/mm 2 ) in duodenum:NP 1240 ± 139.4, MP 1070 ± 154.7 vs 458 ± 50.39 (P ileum:NP 818.60 ± 123.0, MP 640.1 ± 32.75 vs 466.9 ± 22.4 (P in comparison to the control group, the groups receiving TiO 2 showed a statistically significant increase in the levels of the inflammatory cytokines iL-12, iL-4, iL-23, TNF-α, iFN-γ and TGF-β. The cytokine production was more pronounced in the ileum (mean ± SE):iL-12: NP 33.98 ± 11.76, MP 74.11 ± 25.65 vs 19.06 ± 3.92 (P iL-4: NP 17.36 ± 9.96, MP 22.94 ± 7.47 vs 2.19 ± 0.65 (P iL-23: NP 157.20 ± 75.80, MP 134.50 ± 38.31 vs 22.34 ± 5.81 (P iFNγ: NP 15.85 ± 9.99, MP 34.08 ± 11.44 vs 2.81 ±

关键词：钛二氧化物 Microparticles Nanoparticles 有免疫力的反应小肠老鼠

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Prevalence of celiac disease in an urban area of Brazil with predominantly European ancestry

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World Journal of Gastroenterology 2006年第40期12卷 6546-6550页

作者： Maria Angélica G Pereira Carmen L Ortiz-Agostinho iêda nishitokukado Maria N Sato Adérson OMC damio Marília L Alencar Clarice P Abrantes-Lemos Eduardo LR Canado Thales de Brito Sérgio O ioshii Sandra BM Valarini Aytan M Sipahi Department of Gastroenterology Medical Investigation Laboratories (LIM 07) University of So Paulo School of Medicine So Paulo SP Brazil Medical investigation Laboratories (LiM 56) University of So Paulo School of Medicine So Paulo SP Brazil institute of Tropical Medicine (LiM 06) University of So Paulo School of Medicine So Paulo SP Brazil Department of Histopathology Federal University of Paraná Brazil Department of internal Medicine Catholic University of Paraná Brazil

AiM: To determine the prevalence of celiac disease in a group of volunteer blood donors at a blood bank in the city of Curitiba, Brazil through detection of the serum marker immunoglobulin A (igA) antitransglutaminase ***: Blood samples collected from 2086 healthy subjects at the Paraná State Center for Hematology and Hemotherapy in Curitiba were submitted to ELiSA testing for the igA antitransglutaminase antibody. Positive samples received igA antiendomysium antibody test through indirect immunofluorescence using human umbilical cord as substrate. Subsequently, patients who were positive on both tests underwent small bowel (distal duodenum) ***: Six subjects, four males and two females, tested positive for the two serum markers. Five of the six were submitted to intestinal biopsy (one declined the procedure). Biopsy results revealed changes in the distal duodenum mucosa (three classified as Marsh Ⅲb lesions and two as Marsh Ⅱ lesions). Most donors diagnosed having celiac disease presented multiple symptoms (gastrointestinal tract complaints). One donor reported having a family history of celiac disease (in a niece).CONCLUSiON: Among apparently healthy blood donors,the prevalence of biopsy-confirmed celiac disease was approximately 1:417, similar to that seen in European countries.

关键词：腹腔疾病病理治疗临床

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