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Resolving the Mortierellaceae phylogeny through synthesis of multi-gene phylogenetics and phylogenomics

Fungal Diversity 2020年第5期104卷 267-289页

作者： natalie Vandepol Julian Liber Alessandro Desirò hyunsoo na Megan Kennedy Kerrie Barry Igor V.Grigoriev Andrew N.Miller Kerry O’Donnell Jason E.Stajich Gregory Bonito Department of Microbiology and Molecular Genetics Michigan State UniversityEast LansingMI 48824USA Department of Plant Biology Michigan State UniversityEast LansingMI 48824USA Department of Plant Soil and Microbial Sciences Michigan State UniversityEast LansingMI 48824USA Joint Genome Institute BerkeleyCA 94720USA Illinois natural History Survey University of Illinois Urbana-ChampaignChampaignIL 61820USA United States Department of Agriculture Agricultural Research ServicePeoriaIL 61604USA Department of Microbiology and Plant Pathology&Institute for Integrative Genome Biology University of California-RiversideRiversideCA 92521USA

Early efforts to classify Mortierellaceae were based on macro-and micromorphology,but sequencing and phylogenetic studies with ribosomal Dna(rDna)markers have demonstrated conflicting taxonomic groupings and polyphyletic *** some taxonomic confusion in the family has been clarified,rDna data alone is unable to resolve higher level phylogenetic relationships within *** this study,we applied two parallel approaches to resolve the Mortierel-laceae phylogeny:low coverage genome(LCG)sequencing and high-throughput,multiplexed targeted amplicon sequenc-ing to generate sequence data for multi-gene *** then combined our datasets to provide a well-supported genome-based phylogeny having broad sampling depth from the amplicon *** the Mortierellaceae phylogeny into monophyletic genera resulted in 13 genera,7 of which are newly ***-coverage genome sequencing proved to be a relatively cost-effective means of generating a high-confidence *** multi-gene phylogenetics approach enabled much greater sampling depth and breadth than the LCG approach,but has limitations *** present this work to resolve some of the taxonomic confusion and provide a genus-level framework to empower future studies on Mortierellaceae diversity and evolution.

关键词： Mortierellaceae Phylogenomics Molecular systematics Taxonomy Multi-locus sequence typing

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Massively parallel electro-optic sampling of space-encoded optical pulses for ultrafast multi-dimensional imaging

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Light(Science & Applications) 2023年第2期12卷 304-313页

作者： Yongjin na hyunsoo Kwak Changmin Ahn Seung Eon Lee Woojin Lee Chu-Shik Kang Jungchul Lee Junho Suh Hongki Yoo Jungwon Kim Korea Advanced Institute of Science and Technology(KAIST) Daejeon 34141Korea Korea Research Institute of Standards and Science(KRISS) Daejeon 34113Korea

High-speed and high-resolution imaging of surface profiles is critical for the investigation of various structures and mechanical dynamics of micro-and nano-scale *** particular,recent emergence of various nonlinear,transient and complex mechanical dynamics,such as anharmonic vibrations in mechanical resonators,has necessitated real-time surface deformation imaging with higher axial and lateral resolutions,speed,and dynamic ***,real-time capturing of fast and complex mechanical dynamics has been challenging,and direct time-domain imaging of displacements and mechanical motions has been a missing element in studying full-field structural and dynamic ***,by exploiting the electro-optic sampling with a frequency comb,we demonstrate a line-scan time-of-flight(TOF)camera that can simultaneously measure the TOF changes of more than 1000 spatial coordinates with hundreds megapixels/s pixel-rate and sub-nanometre axial resolution over several millimetres *** unique combination of performances enables fast and precise imaging of both complex structures and dynamics in three-dimensional devices and mechanical resonators.

关键词： deformation dimensional dynamics

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