检索结果-南通市图书馆

Effect of loureirin A against Candida albicans biofilms

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Journal of Natural Medicines 2019年第8期17卷 616-623页

作者： LIN Mei-Yu YUAN Zhong-Lan HU Dan-Dan HU Gan-Hai ZHANG ri-Li ZHONG Hua YAN Lan JIANG Yuan-Ying su Juan WANG Yan School of Pharmacy Naval Military Medical University Shanghai 200433 China Department of Pharmacy Fujian University of Traditional Chinese Medicine Fuzhou 350000 China

Loureirin A is a major active component of Draconis sanguis, a traditional Chinese medicine. This work aimed to investigate the activity of loureirin A against Candida albicans biofilms. 2, 3-Bis-(2-methoxy-4-nitro-5-sulfophenyl)-2 H-tetrazolium-5-carboxanilide(XTT) reduction assay and scanning electron microscopy were used to investigate the anti-biofilm *** inhibitory concentration testing and time-kill curve assay were used to evaluate fungicidal activity. Cell surface hydrophobicity(CSH) assay and hyphal formation experiment were respectively carried out to investigate adhesion and morphological transition,two virulence traits of C. albicans. Real-time RT-PCR was used to investigate gene expression. Galleria mellonella-C. albicans and Caenorhabditis elegans-C. albicans infection models were used to evaluate the in-vivo antifungal effect. Human umbilical vein endothelial cells and C. elegans nematodes were used to evaluate the toxicity of loureirin A. Our data indicated that loureirin A had a significant effect on inhibiting C. albicans biofilms, decreasing CSH, and suppressing hyphal formation. Consistently, loureirin A down-regulated the expression of some adhesion-related genes and hypha/biofilm-related genes. Moreover, loureirin A prolonged the survival of Galleria mellonella and Caenorhabditis elegans in C. albicans infection models and exhibited low toxicity. Collectively,loureirin A inhibits fungal biofilms, and this effect may be associated with the suppression of pathogenic traits, adhesion and hyphal formation.

关键词： Candida albicans Loureirin A Anti-biofilm Morphological transition

Entacapone promotes hippocampal neurogenesis in mice

在线全文

学校读者我要写书评

暂无评论

Neural Regeneration Research 2021年第6期16卷 1005-1010页

作者： Dae Young Yoo Hyo Young Jung Woosuk Kim Kyu ri Hahn hyun Jung Kwon sung Min Nam Jin Young Chung Yeo sung Yoon Dae Won Kim In Koo Hwang Department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea Department of Anatomy College of MedicineSoonchunhyang UniversityCheonanSouth Korea Department of Biomedical Sciences and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea Department of Biochemistry and Molecular Biology Research Institute of Oral SciencesCollege of DentistryGangneung-Wonju National UniversityGangneungSouth Korea Department of Anatomy College of Veterinary MedicineKonkuk UniversitySeoulSouth Korea Department of Veterinary Internal Medicine and Geriatrics College of Veterinary MedicineKangwon National UniversityChuncheonSouth Korea

Entacapone,a catechol-O-methyltransferase inhibitor,can strengthen the therapeutic effects of levodopa on the treatment of Parkinson’s ***,few studies are reported on whether entacapone can affect hippocampal neurogenesis in *** investigate the effects of entacapone,a modulator of dopamine,on proliferating cells and immature neurons in the mouse hippocampal dentate gyrus,60 mice(7 weeks old)were randomly divided into a vehicle-treated group and the groups treated with 10,50,or 200 mg/kg *** results showed that 50 and 200 mg/kg entacapone increased the exploration time for novel object *** staining results revealed that after entacapone treatment,the numbers of Ki67-positive proliferating cells,doublecortin-positive immature neurons,and phosphorylated cAMP response element-binding protein(pCREB)-positive cells were significantly *** blot analysis results revealed that treatment with tyrosine kinase receptor B(TrkB)receptor antagonist significantly decreased the exploration time for novel object recognition and inhibited the expression of phosphorylated TrkB and brain-derived neurotrophic factor(BDNF).Entacapone treatment antagonized the effects of TrkB receptor *** results suggest that entacapone treatment promoted hippocampal neurogenesis and improved memory function through activating the BDNF-TrkB-pCREB *** study was approved by the Institutional Animal Care and Use Committee of Seoul National University(approval ***-130730-1)on February 24,2014.

关键词： brain-derived neurotrophic factor entacapone hippocampus neurogenesis neurotrophic factor phosphorylated cAMP response element-binding protein tyrosine kinase receptor B receptor

Body-Shaping Membrane to Regenerate Breast Fat by Elastic Structural Holding

在线全文

学校读者我要写书评

暂无评论

Research 2024年第1期2023卷 165-180页

作者： Hye-Seon Kim Jeongeun Park hyun-su Ha Sewoom Baek Chan Hee Lee Kyubae Lee suji Park Jueun Kim Se Won Yi Hak-Joon sung Department of Medical Engineering Graduate School of Medical ScienceBrain Korea 21 ProjectYonsei University College of MedicineSeoul 03722Republic of Korea TMD LAB Co.Ltd. 6th Floor31Gwangnaru-ro 8-gilSeongdong-guSeoul 04799Republic of Korea

Tissue regeneration requires structural holding and movement support using tissue-type-specific aids such as bone casts,skin bandages,and joint ***,an unmet need exists in aiding breast fat regeneration as the breast moves following continuous body motion by exposing the breast fat to dynamic ***,the concept of elastic structural holding is applied to develop a shape-fitting moldable membrane for breast fat regeneration(“adipoconductive”)after surgical defects are *** membrane has the following key characteristics:(a)It contains a panel of honeycomb structures,thereby efficiently handling motion stress through the entire membrane;(b)a strut is added into each honeycomb in a direction perpendicular to gravity,thereby suppressing the deformation and stress concentration upon lying and standing;and(c)thermo-responsive moldable elastomers are used to support structural holding by suppressing large deviations of movement that occur *** elastomer became moldable upon a temperature shift above T_(m).The structure can then be fixed as the temperature *** a result,the membrane promotes adipogenesis by activating mechanotransduction in a fat miniature model with pre-adipocyte spheroids under continuous shaking in vitro and in a subcutaneous implant placed on the motion-prone back areas of rodents in vivo.

关键词： thereby shaking Elastic

Fabrication and characterization of 3D scaffold using 3D plotting system

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Science Bulletin 2010年第1期55卷 94-98页

作者： LEE Jun-Hee PARK su-A PARK KoEun KIM Jae-hyun KIM Kyung-Shik LEE Jihye Kim WanDoo Bio-Mechatronics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM) Nano-Mechanics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM) Nano-Mechatronics Team Division of Nano-Machinery Korea Institute of Machinery and Materials (KIMM)

In this paper, we design and fabricate a 3D scaffold using rapid prototyping (RP) technology for tissue engineering. The scaffold should have a three-dimensional interconnected pore network. We fabricate a polycaprolactone (PCL) scaffold with interconnecting pores and uniform porosity for cell ingrowth using a 3D plotting system. In order to keep the three dimensional shape under mechanical loading while implanted, we design an oscillating nozzle system to increase elastic modulus and yield strength of PCL strand. We characterize the influence of pore geometry, compressive modulus of the scaffold, elastic modulus and yield strength of the strand using SEM, dynamical mechanical analysis (DMA) and Nano-UTM. Finally the cell responses on scaffolds are observed.

关键词：三维形状绘图系统表征支架制作动态机械分析弹性模量细胞反应

Inhibiting scar formation via wearable multilayer stacked electret patch:Self-creation of persistent and customizable DC electric field for fibrogenic activity restriction

在线全文

学校读者我要写书评

暂无评论

InfoMat 2024年第3期6卷 66-80页

作者： sung-Won Kim sumin Cho Donghan Lee Jiyu hyun sunmin Jang Inwoo Seo hyun su Park Hee Jae Hwang hyung-Seop Han Dae Hyeok Yang Heung Jae Chun suk Ho Bhang Dongwhi Choi School of Chemical Engineering Sungkyunkwan UniversitySuwonRepublic of Korea Department of Mechanical Engineering(Integrated Engineering Program) Kyung Hee UniversityYonginGyeonggiRepublic of Korea Biomaterials Research Center Biomedical Research DivisionKorea Institute of Science and Technology(KIST)SeoulRepublic of Korea Department of Medical Life Sciences College of MedicineInstitute of Cell and Tissue EngineeringThe Catholic University of KoreaSeoulRepublic of Korea

Electrical stimulation has recently received attention as noninvasive treatment in skin wound healing with its outstanding biological property for clinical ***,the complexity of equipment for applying appropriate electrical stimulation remains an ongoing ***,we proposed a strategy for skin scar inhibition by providing electrical stimulation via a multilayer stacked electret(MS-electret),which can generate direct current(DC)electric field(EF)without any power supply *** addition,the MS-electret can easily control the intensity of EFs by simply stacking electret layers and maintain stable EF with the surface potential of 3400 V over 5 days owing to the injected charges on the electret *** confirmed inhibition of type 1 collagen andα-SMA expression of human dermal fibroblasts(hDFs)by 90%and 44%in vitro,indicating that the transition of hDFs to myofibroblasts was restricted by applying stable electrical *** further revealed a 20%significant decrease in the ratio of myofibroblasts caused by the MS-electret in *** findings present that the MS-electret is an outstanding candidate for effective skin scar inhibition with a battery-free,physiological electrical microenvironment,and noninvasive treatment that allows it to prevent external infection.

关键词： DC electric field dermal fibroblasts electret scar formation restriction self-creation of electrical stimulation

Is a split-dose regimen of 2 L polyethylene glycol plus ascorbic acid tolerable for colonoscopy in an early morning visit to a comprehensive medical check-up?

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2017年第6期23卷 1030-1037页

作者： Ji Yeon Seo Changhyun Lee Eun Hyo Jin Mi hyun Yun Joo hyun Lim Hae Yeon Kang Jong In Yang su Jin Chung sun Young Yang Joo sung Kim Department of Internal Medicine and Healthcare Research Institute Healthcare System Gangnam CenterSeoul National University Hospital Department of Nursing Healthcare System Gangnam CenterSeoul National University Hospital

To evaluate the effectiveness and tolerability of a split-dose 2 L polyethylene glycol (PEG)/ascorbic acid (AA) regimen for healthy examinees who visited for comprehensive medical check-up in the early *** February 2015 to March 2015, examinees of average risk who were scheduled for a colonoscopy in the morning were retrospectively *** 189 examinees were divided into split-dose and non-split-dose groups. The adequacy of bowel preparation for the split-dose group vs the non-split-dose group was 96.8% vs 85.2%, respectively, P riction was 74.6% vs 58.2%, respectively, P riate analysis (HR = 10.89, 95%CI: 6.53-18.17, P riction without any difference in satisfaction and discomfort. Introducing a split-dose regimen of 2 L PEG/AA to morning colonoscopy examinees is effective and tolerable in a comprehensive medical check-up setting.

关键词： Compliance Early morning colonoscopy Effectiveness Split-dose regimen Tolerability

Analysis of Brucella cellular fatty acids

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Frontiers of Medicine 2010年第2期4卷 216-219页

作者： Zhen-Xiang ZHAO Hai JIANG Bu-Yun CUI Hong-Yan ZHAO Dong-ri PIAO Lan-Yu LI su-Zhen HAO Department of Microbiology and Immunology Shanxi Medical UniversityTaiyuan 030001China National Institute for Communicable Disease Control and Prevention Chinese Center for Disease Control and PreventionBeijing 102206China

To acquire data of Brucella cellular fatty acids(CFAs)and probe into the possibility of utilizing CFAs information in typing Brucella,19 reference strains were subjected to CFAs *** all strains were inoculated on Brucella Agar plates,the cells were harvested,saponiﬁcated,methylated and extracted to provide fatty acid methylesters for gas chromatography(GC)*** on the CFAs data matrix,a dendrogram of 19 reference strains was generated by SPSS11.5 software *** results showed that 19 reference strains were divided intoﬁve clusters:cluster 1 included ***(bv.1,2,3,5)and ***;cluster 2 included ***(bv.3,4,5,6)and ***(bv.1,2,3);cluster 3 included ***(bv.1,2,7,9)and ***;cluster 4 was ***(bv.4);and cluster 5 was *** Brucella by GC analysis of CFAs is a good method to reﬂect drug resistance of Brucella,and the classiﬁcation is beneﬁcial for clinical *** also provides a new result of typing and demonstrates that the traditional classiﬁcation is not completely *** analysis may identify ***(bv.4)and ***.

关键词： Brucella gas chromatography cellular fatty acids dendrogram typing

Immunostimulatory effect of ethanol extract of Chondracanthus tenellus in RAW 264.7 macrophages in vitro

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Asian Pacific Journal of Tropical Biomedicine 2021年第6期11卷 263-272页

作者： Cheol Park Da Hye Kwon Hyesook Lee su hyun Hong Gi-Young Kim Hee-Jae Cha Do-hyung Kim suhkmann Kim Heui-Soo Kim Hye-Jin Hwang Yung hyun Choi Division of Basic Sciences College of Liberal StudiesDong-eui UniversityBusan 47340Republic of Korea Anti-Aging Research Center Dong-eui UniversityBusan 47340Republic of Korea Department of Biochemistry College of Korean MedicineDong-eui UniversityBusan 47227Republic of Korea Department of Marine Life Sciences School of Marine Biomedical SciencesJeju National UniversityJeju 63243Republic of Korea Department of Parasitology and Genetics College of MedicineKosin UniversityBusan 49104Republic of Korea Department of Aquatic Life Medicine College of Fisheries SciencePukyong National UniversityBusan 48513Republic of Korea Department of Chemistry College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Biological Sciences College of Natural SciencesPusan National UniversityBusan 46241Republic of Korea Department of Food and Nutrition College of NursingHealthcare Sciences&Human EcologyDong-eui UniversityBusan 47340Republic of Korea

Objective:To investigate whether ethanol extracts of Chondracanthus tenellus(EECT)could improve immunomodulatory property of murine monocyte/macrophage RAW 264.7 ***:Cell viability,phagocytic ability,and nitric oxide were *** levels of prostaglandin E2 and cytokines were determined using enzyme-linked immunosorbent assay *** of immunoregulatory response protein was detected by Western blotting ***:As the concentration of EECT increased,the morphology of the cells changed to a typical active macrophage shape,and the phagocytic activity increased *** also effectively enhanced the production and secretion of immunomodulatory mediators,such as nitric oxide and prostaglandin E2,and *** addition,compared with the control group,EECT markedly stimulated the expression of Toll-like receptor 4(TLR4)and myeloid differentiation factor 88,one of the TLR4 adapter ***,EECT promoted the nucleus translocation of nuclear factor-kappa B(NF-κB)by increasing the phosphorylation and degradation of the inhibitor of NF-κB-α,indicating activation of the NF-κB signaling ***,similar trends were found in cells treated with lipopolysaccharide as a positive ***:Taken together,the results indicate that EECT has an immunomodulatory effect by increasing the production of immunomodulatory mediators and cytokines through activation of the TLR4/NF-κB signaling *** could be used as a potential candidate for medication or dietary supplements to increase immune activity.

关键词： Chondracanthus tenellus Immunomodulatory property TLR4 NF-κB

Developmental process of the arcuate fasciculus from infancy to adolescence: a diffusion tensor imaging study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Neural Regeneration Research 2016年第6期11卷 937-943页

作者： Hyeong Jun Tak Jin hyun Kim su Min Son Department of Physical Medicine and Rehabilitation School of Medicine Yeungnam University

We investigated the radiologic developmental process of the arcuate fasciculus（AF） using subcomponent diffusion tensor imaging（DTI） analysis in typically developing volunteers. DTI data were acquired from 96 consecutive typically developing children, aged 0–14 years. AF subcomponents, including the posterior, anterior, and direct AF tracts were analyzed. success rates of analysis（AR） and fractional anisotropy（FA） values of each subcomponent tract were measured and compared. AR of all subcomponent tracts, except the posterior, showed a significant increase with aging（P 〈 0.05）. subcomponent tracts had a specific developmental sequence： First, the posterior AF tract, second, the anterior AF tract, and last, the direct AF tract in identical hemispheres. FA values of all subcomponent tracts, except right direct AF tract, showed correlation with subject＇s age（P 〈 0.05）. Increased AR and FA values were observed in female subjects in young age（0–2 years） group compared with males（P 〈 0.05）. The direct AF tract showed leftward hemispheric asymmetry and this tendency showed greater consolidation in older age（3–14 years） groups（P 〈 0.05）. These findings demonstrated the radiologic developmental patterns of the AF from infancy to adolescence using subcomponent DTI analysis. The AF showed a specific developmental sequence, sex difference in younger age, and hemispheric asymmetry in older age.

关键词： tracts tensor younger older developmental maturation typically asymmetry anisotropy fractional