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Current status and challenges for cell-cultured milk technology: a systematic review

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Journal of Animal Science and Biotechnology 2024年第5期15卷 1778-1792页

作者： Hyuk Cheol kwon hyun Su jung Vahinika Kothuri Sung Gu Han Department of Food Science and Biotechnology of Animal Resources Konkuk UniversitySeoulRepublic of Korea

Cellular agriculture is an innovative technology for manufacturing sustainable agricultural products as an alternative to traditional *** most cellular agriculture is predominantly centered on the production of cultured meat,there is a growing demand for an understanding of the production techniques involved in dairy products within cellular *** review focuses on the current status of cellular agriculture in the dairy sector and technical challenges for cell-cultured milk *** agriculture technology in the dairy sector has been classified into fermentation-based and animal cell culture-based cellular ***,various companies synthesize milk components through precision fermentation ***,several startup companies are pursuing animal cell-based technology,driven by public concerns regarding genetically modified organisms in precision fermentation ***,this review offers an up-to-date exploration of animal cell-based cellular agriculture to produce milk components,specifically emphasizing the structural,functional,and productive aspects of mammary epithelial cells,providing new information for industry and academia.

关键词： Cell culture system Cell-cultured milk Mammary epithelial cells Precision fermentation

Entacapone promotes hippocampal neurogenesis in mice

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Neural Regeneration Research 2021年第6期16卷 1005-1010页

作者： Dae Young Yoo Hyo Young jung Woosuk Kim Kyu Ri Hahn hyun jung kwon Sung Min Nam Jin Young Chung Yeo Sung Yoon Dae Won Kim In Koo Hwang Department of Anatomy and Cell Biology College of Veterinary Medicineand Research Institute for Veterinary ScienceSeoul National UniversitySeoulSouth Korea Department of Anatomy College of MedicineSoonchunhyang UniversityCheonanSouth Korea Department of Biomedical Sciences and Research Institute for Bioscience and BiotechnologyHallym UniversityChuncheonSouth Korea Department of Biochemistry and Molecular Biology Research Institute of Oral SciencesCollege of DentistryGangneung-Wonju National UniversityGangneungSouth Korea Department of Anatomy College of Veterinary MedicineKonkuk UniversitySeoulSouth Korea Department of Veterinary Internal Medicine and Geriatrics College of Veterinary MedicineKangwon National UniversityChuncheonSouth Korea

Entacapone,a catechol-O-methyltransferase inhibitor,can strengthen the therapeutic effects of levodopa on the treatment of Parkinson’s ***,few studies are reported on whether entacapone can affect hippocampal neurogenesis in *** investigate the effects of entacapone,a modulator of dopamine,on proliferating cells and immature neurons in the mouse hippocampal dentate gyrus,60 mice(7 weeks old)were randomly divided into a vehicle-treated group and the groups treated with 10,50,or 200 mg/kg *** results showed that 50 and 200 mg/kg entacapone increased the exploration time for novel object *** staining results revealed that after entacapone treatment,the numbers of Ki67-positive proliferating cells,doublecortin-positive immature neurons,and phosphorylated cAMP response element-binding protein(pCREB)-positive cells were significantly *** blot analysis results revealed that treatment with tyrosine kinase receptor B(TrkB)receptor antagonist significantly decreased the exploration time for novel object recognition and inhibited the expression of phosphorylated TrkB and brain-derived neurotrophic factor(BDNF).Entacapone treatment antagonized the effects of TrkB receptor *** results suggest that entacapone treatment promoted hippocampal neurogenesis and improved memory function through activating the BDNF-TrkB-pCREB *** study was approved by the Institutional Animal Care and Use Committee of Seoul National University(approval ***-130730-1)on February 24,2014.

关键词： brain-derived neurotrophic factor entacapone hippocampus neurogenesis neurotrophic factor phosphorylated cAMP response element-binding protein tyrosine kinase receptor B receptor

Novel Rifle Number Recognition Based on Improved YOLO in Military Environment

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Computers, Materials & Continua 2024年第1期78卷 249-263页

作者： hyun kwon Sanghyun Lee Department of Artificial Intelligence and Data Science Korea Military AcademySeoulKorea Graduate School of Information Security Korea Advanced Institute of Science and TechnologyDaejeonKorea

Deep neural networks perform well in image recognition,object recognition,pattern analysis,and speech *** military applications,deep neural networks can detect equipment and recognize *** military equipment,it is necessary to detect and recognize rifle management,which is an important piece of equipment,using deep neural *** have been no previous studies on the detection of real rifle numbers using real rifle image *** this study,we propose a method for detecting and recognizing rifle numbers when rifle image data are *** proposed method was designed to improve the recognition rate of a specific dataset using data fusion and transfer *** the proposed method,real rifle images and existing digit images are fusedas trainingdata,andthe final layer is transferredto theYolov5 *** detectionand recognition performance of rifle numbers was improved and analyzed using rifle image and numerical *** used actual rifle image data(K-2 rifle)and numeric image datasets,as an experimental *** was used as the machine learning *** results show that the proposed method maintains 84.42% accuracy,73.54% precision,81.81% recall,and 77.46% F1-score in detecting and recognizing rifle *** proposed method is effective in detecting rifle numbers.

关键词： Machine learning deep neural network rifle number recognition detection

Adverse effects of early-life stress:focus on the rodent neuroendocrine system

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Neural Regeneration Research 2024年第2期19卷 336-341页

作者： Seung hyun Lee Eui-Man jung Department of Molecular Biology College of Natural SciencesPusan National UniversityBusanRepublic of Korea

Early-life stress is associated with a high prevalence of mental illnesses such as post-traumatic stress disorders,attention-deficit/hyperactivity disorder,schizophrenia,and anxiety or depressive behavior,which constitute major public health *** the early stages of brain development after birth,events such as synaptogenesis,neuron maturation,and glial differentiation occur in a highly orchestrated manner,and external stress can cause adverse long-term effects throughout *** body utilizes multifaceted mechanisms,including neuroendocrine and neurotransmitter signaling pathways,to appropriately process external *** individuals first exposed to early-life stress deploy neurogenesis as a stress-defense mechanism;however,in adulthood,early-life stress induces apoptosis of mature neurons,activation of immune responses,and reduction of neurotrophic factors,leading to anxiety,depression,and cognitive and memory *** process involves the hypothalamus-pituitary-adrenal axis and neurotransmitters secreted by the central nervous system,including norepinephrine,dopamine,and *** rodent early-life stress model is generally used to experimentally assess the effects of stress during *** paper reviews the use of the early-life stress model and stress response mechanisms of the body and discusses the experimental results regarding how early-life stress mediates stress-related pathways at a high vulnerability of psychiatric disorder in adulthood.

关键词： early-life stress hypothalamic-pituitary-adrenergic axis maternal separation mental illness neurodevelopmental disorder neuroendocrine system neurotransmitter

Unraveling the role of METTL14 in metabolic dysfunction-associated fatty liver disease:insights and therapeutic implications

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Signal Transduction and Targeted Therapy 2024年第5期9卷 1781-1783页

作者： So jung Kim Jeongeun hyun Institute of Tissue Regeneration Engineering(ITREN) Dankook UniversityCheonanRepublic of Korea Department of Nanobiomedical Science and BK21 PLUS NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonanRepublic of Korea Mechanobiology Dental Medicine Research Center College of DentistryDankook UniversityCheonanRepublic of Korea

The recent article published in Signal Transduction and Targeted Therapy sheds light on the significance of N6-methyladenosine(m6A)methyltransferase-like protein METTL14 in mitigating the progression of metabolic dysfunction-associated fatty liver disease(MAFLD).1 In this study,Wang et *** the downregulation of METTL14 in hepatocytes from both MAFLD patients and high-fat diet(HFD)-induced MAFLD mouse models,underscoring its pivotal role in maintaining hepatic lipid and redox homeostasis in normal livers.

关键词： liver homeostasis al.

Enhancing Identity Protection in Metaverse-Based Psychological Counseling System

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Computers, Materials & Continua 2024年第1期78卷 617-632页

作者： Jun Lee Hanna Lee Seong Chan Lee hyun kwon Department of Game Software Hoseo UniversityAsan-si31499Korea Yatav Enter Seoul04799Korea Department of Artificial Intelligence and Data Science Korea Military AcademySeoul01805Korea

Non-face-to-face psychological counseling systems rely on network technologies to anonymize information regard-ing client ***,these systems often face challenges concerning voice data leaks and the suboptimal communication of the client’s non-verbal expressions,such as facial cues,to the *** study proposes a metaverse-based psychological counseling system designed to enhance client identity protection while ensuring efficient information delivery to counselors during non-face-to-face *** proposed systemincorporates a voicemodulation function that instantlymodifies/masks the client’s voice to safeguard their ***,it employs real-time client facial expression recognition using an ensemble of decision trees to mirror the client’s non-verbal expressions through their avatar in the metaverse *** system is adaptable for use on personal computers and smartphones,offering users the flexibility to access metaverse-based psychological counseling across diverse *** performance evaluation of the proposed system confirmed that the voice modulation and real-time facial expression replication consistently achieve an average speed of 48.32 frames per second or higher,even when tested on the least powerful smartphone ***,a total of 550 actual psychological counseling sessions were conducted,and the average satisfaction rating reached 4.46 on a 5-point *** indicates that clients experienced improved identity protection compared to conventional non-face-to-face metaverse counseling ***,the counselor successfully addressed the challenge of conveying non-verbal cues from clients who typically struggled with non-face-to-face psychological *** proposed systemholds significant potential for applications in interactive discussions and educational activities in the metaverse.

关键词： Metaverse counseling system face tracking identity protection

Generation of double knockout cattle via CRISPR-Cas9 ribonucleoprotein(RNP)electroporation

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Journal of Animal Science and Biotechnology 2024年第1期15卷 456-462页

作者： Gyeong-Min Gim Kyeong-Hyeon Eom Dong-Hyeok kwon Dae-Jin jung Dae-hyun Kim Jun-Koo Yi Jae-jung Ha Ji-hyun Lee Seong-Beom Lee Woo-Jae Son Soo-Young Yum Won-Wu Lee Goo Jang Laboratory of Theriogenology and Biotechnology Department of Veterinary Clinical ScienceCollege of Veterinary Medicine and the Research Institute of Veterinary ScienceSeoul National UniversitySeoulRepublic of Korea LARTBio Co. Ltd.SeoulRepublic of Korea Gyeongsangbukdo Livestock Research Institute YeongjuRepublic of Korea Comparative Medicine Disease Research Center Seoul National UniversitySeoulRepublic of Korea

Background Genome editing has been considered as powerful tool in agricultural ***,genome editing progress in cattle has not been fast as in other mammal species,for some disadvantages including long gestational periods,single pregnancy,and high raising ***,technically demanding methods such as microinjection and somatic cell nuclear transfer(SCNT)are needed for gene editing in *** this point of view,electroporation in embryos has been risen as an *** First,editing efficiency of our electroporation methods were tested for *** of mutation on embryo was confirmed by T7E1 *** first combination,mutation rates for MSTN and PRNP were 57.6%±13.7%and 54.6%±13.5%,*** case of MSTN/BLG,mutation rates were 83.9%±23.6%for MSTN,84.5%±18.0%for ***,the double-KO embryos were transferred to surrogates and mutation rate was identified in resultant calves by targeted deep *** recipients were transferred for MSTN/PRNP,4 calves were delivered,and one calf underwent an induction for double *** surrogates were given double-KO embryos for MSTN/BLG,and four of the six calves that were born had mutations in both *** These data demonstrated that production of genome edited cattle via electroporation of RNP could be effectively ***,MSTN and PRNP from beef cattle and MSTN and BLG from dairy cattle have been born and they will be valuable resources for future precision breeding.

关键词： Beta-lactoglobulin Cattle CRISPR-Cas9 Electroporation Knockout MSTN PRNP

Severe thrombocytopenia before liver transplantation is associated with delayed recovery of thrombocytopenia regardless of donor type

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World Journal of Gastroenterology 2008年第37期14卷 5723-5729页

作者： Jae Hyuck Chang Jong Young Choi hyun Young Woo jung hyun kwon Chan Ran You Si hyun Bae Seung Kew Yoon Myung-Gyu Choi In-Sik Chung Dong Goo Kim Department of Internal Medicine College of Medicine the Catholic University of Korea505 Banpo-Dong Seocho-Gu Seoul 137-040 Korea Department of Surgery College of Medicine the Catholic University of Korea505 Banpo-Dong Seocho-Gu Seoul 137-040 Korea

AIM： To compare the recovery of thrombocytopenia and splenomegaly during long-term follow-up after liver transplantation in patients receiving a living donor transplant or a cadaveric donor transplant. METHODS： This was a retrospective cohort study of 216 consecutive liver transplant patients who survived for 〉 6 mo after transplantation; 169 received a liver transplant from a living donor and 47 from a cadaveric donor. The platelet counts or spleen volumes were examined before transplant, i, 6, and 12 mo after transplant, and then annually until 5 years after transplant. RESULTS： The mean follow-up period was 49 mo （range, 21-66）. Platelet counts increased continuously for 5 years after orthotopic liver transplant. The restoration of platelet counts after transplant was significantly slower in patients with severe pretransplant thrombocytopenia （〈 50000/μL） until 4 years after transplant （P = 0.005）. Donor type did not significantlyaffect the recovery of platelet count and spleen volume in either patient group. In multivariate analysis, pretransplant severe thrombocytopenia （〈 50000/μL） was an independent factor associated with sustained thrombocytopenia （P 〈 0.001, odds ratio 6.314; confidence interval, 2.828-14.095）. Thrombocytopenia reappeared after transplant in seven patients with portal flow disturbance near the anastomosis site. CONCLUSION： Our study suggests that severe thrombocytopenia before transplant is closely associated with delayed recovery of platelet count after transplant and donor type did not affect the recovery of thrombocytopenia. The reappearance of thrombocytopenia after transplant should be considered a possible indicator of flow disturbance in the portal vein.

关键词： Liver transplantation Thrombocytopenia Splenomegaly Hypersplenism

Combined Insights from Leachate Structure and Microstructure Characteristics for Eating Quality of Convenience Rice Processed by Super-Heated and Pressurized Steam Technologies

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Rice science 2024年第4期31卷 475-488,I0035,I0036页

作者： Mingyo HA Hyo-Young JEONG Ju Hun LEE hyun-jung CHUNG Division of Food and Nutrition Chonnam National UniversityGwangju 61186South Korea Department of Biotechnology College of Life Sciences and BiotechnologyKorea UniversitySeoul 02841South Korea Division of Food Science&Culinary Arts Shinhan UniversityGyeonggi 11644South Korea

Convenience rice has become widely popular due to its easy availability for cooking. This study investigated the starch structure and composition of leachate and the microstructure of reheated convenience rice using novel processing technologies: super-heated steaming(SHS), auto-electric cooking(AEC), and pressurized-steam cooking(PSC). Additionally, the effect of two different target water contents(58% and 63%) was also evaluated. The PSC_63% sample had the highest total solids and amylopectin amount in the leachate. The amylopectin amount in the leachate differed significantly based on the targeted water content. Morphological characterization revealed that the swelling of starch and the coated layer on the surface of rice grains were most pronounced in the PSC_63% sample due to the pressure processing. The textural hardness of the AEC_58% sample was much higher than that of the other samples. The PSC_63% sample had the highest textural adhesiveness value, which can be attributed to the highest amylopectin amount in the leachate. Sensory characterization showed that the PSC_63% sample had the highest glossiness, whiteness, moistness, and overall acceptability. The principal component analysis score plots presented substantial differences in the leachate and textural and sensory characteristics of reheated convenience rice among the different processing technologies.

关键词： reheated convenience rice starch structure leachate multi-scale structure eating quality processing technology