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Efficacy of stool multiplex polymerase chain reaction assay in adult patients with acute infectious diarrhea

World Journal of Clinical Cases 2020年第17期8卷 3708-3717页

作者： Jae Sung Ahn Seung In Seo Jinseob kim Taewan kim Jin Gu Kang hyoung Su kim Woon Geon Shin Myoung Kuk Jang Hak Yang kim Department of Internal Medicine Kangdong Sacred Heart HospitalSeoul KS013South Korea Department of Internal Medicine Kangdong Sacred Heart HospitalHallym University College of MedicineSeoul KS013South Korea Institute for Liver and Digestive Diseases Hallym UniversityChuncheon KS013South Korea Department of Epidemiology School of Public HealthSeoul National UniversitySeoul KS013South Korea

BACKGROUND Recently,stool multiplex polymerase chain reaction(PCR)tests have been developed for identifying diarrhea-causing bacterial ***,fecal calprotectin is a well-known effective marker for intestinal mucosal *** To evaluate the efficacy of stool multiplex PCR and fecal calprotectin in acute infectious *** Overall,400 patients with acute infectious diarrhea were enrolled from Kangdong Sacred Heart Hospital(January 2016 to December 2018).Multiplex PCR detected 7 enteropathogenic bacteria including Salmonella,Campylobacter,Shigella,Escherichia coli O157:H7,Aeromonas,Vibrio,and Clostridium *** reviewed clinical and laboratory findings using stool multiplex *** Stool multiplex PCR test detected considerably more bacterial pathogens than stool culture(49.2%vs 5.2%),with Campylobacter as the most common pathogen(54%).Patients with positive stool PCR showed elevated fecal calprotectin expression compared to patients with negative stool PCR(1124.5±816.9 mg/kg vs 609±713.2 mg/kg,P=0.001).C-reactive protein(OR=1.01,95%CI:1.001-1.027,P=0.034)and sigmoidoscopy-detected colitis(OR=4.76,95%CI:1.101-20.551,P=0.037)were independent factors in stool PCR-based detection of bacterial *** and specificity of calprotectin were evaluated to be 70.5%and 60.9%,respectively(adjusted cut-off value=388 mg/kg).CONCLUSION Stool multiplex PCR test has increased sensitivity in detecting pathogens than conventional culture,and it is correlated with calprotectin *** multiplex PCR and calprotectin may be effective in predicting clinical severity of infectious diarrhea.

关键词： Acute infectious diarrhea Stool multiplex polymerase chain reaction Calprotectin

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Silica-quaternary ammonium “Fixed-Quat” nanofilm coated fiberglass mesh for water disinfection and harmful algal blooms control

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Journal of Environmental Sciences 2019年第8期31卷 213-224页

作者： Daniela Diaz Jared Church Mikaeel Young Keug Tae kim Jungsu Park Yun Bin Hwang Swadeshmukul Santra Woo hyoung Lee Department of Civil Environmental and Construction Engineering University of Central Florida NanoScience Technology Center University of Central Florida Burnett School of Biomedical Sciences University of Central Florida Department of Environmental & Energy Engineering Suwon University K-water Institute Korea Water Resources Corporation Department of Material Science and Engineering 4000 Central Florida Blvd University of Central Florida Department of Chemistry 4111 Libra Drive University of Central Florida

Intensification of pollution loading worldwide has promoted an escalation of different types of disease-causing microorganisms, such as harmful algal blooms(HABs), instigating detrimental impacts on the quality of receiving surface waters. Formation of unwanted disinfection by-products(DBPs) resulting from conventional disinfection technologies reveals the need for the development of new sustainable alternatives. Quaternary Ammonium Compounds(QACs) are cationic surfactants widely known for their effective biocidal properties at the ppm level. In this study, a novel silica-based antimicrobial nanofilm was developed using a composite of silica-modified QAC(Fixed-Quat) and applied to a fiberglass mesh as an active surface via sol–gel technique. The synthesized Fixed-Quat nanocoating was found to be effective against E. coli with an inactivation rate of 1.3 × 10^(-3) log reduction/cm min. The Fixed-Quat coated fiberglass mesh also demonstrated successful control of Microcystis aeruginosa with more than 99% inactivation after 10 hr of *** developed antimicrobial mesh was also evaluated with wild-type microalgal species collected in a water body experiencing HABs, obtaining a 97% removal efficiency. Overall,the silica-functionalized Fixed-Quat nanocoating showed promising antimicrobial properties for water disinfection and HABs control, while decreasing concerns related to DBPs formation and the possible release of toxic nanomaterials into the environment.

关键词： Fiberglass mesh HAB mitigation Nanocoating Quaternary ammonium compounds(QACs) Silica-functionalization Water disinfection

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Laparoscopic partial cystectomy with mucosal stripping of extraluminal duodenal duplication cysts

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World Journal of Gastroenterology 2014年第4期20卷 1123-1126页

作者： Jeik Byun hyoung-Min Oh Soo-Hong kim Hyun-Young kim Sung-Eun Jung Kwi-Won Park Woo-Sun kim Department of Pediatric Surgery Seoul National University Children’s Hospital Department of Radiology Seoul National University Hospital

Duodenal duplication cysts are rare congenital *** duplication should be considered in the differential diagnosis of patients who present with abdominal symptoms with cystic structures neighboring the ***,we present an 8-year-old girl with a duodenal duplication cyst treated with partial cystectomy with mucosal stripping performed *** surgery can be considered as a treatment option for duodenal duplication cysts,especially in extraluminal locations.

关键词： Duodenal duplication cyst Laparoscopic surgery Partial cystectomy Extraluminal situation Children

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Energy-efficient digital and wireless IC design for wireless smart sensing

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Journal of Semiconductors 2017年第10期38卷 30-38页

作者： Jun Zhou Xiongchuan Huang Chao Wang Tony Tae-hyoung kim Yong Lian University of Electronic Science and Technology of China Broadcom Singapore University of Technology and Design Nanyang Technological University Shanghai Jiao Tong University

Wireless smart sensing is now widely used in various applications such as health monitoring and structural *** conventional wireless sensor nodes,significant power is consumed in wirelessly transmitting the raw *** sensing adds local intelligence to the sensor node and reduces the amount of wireless data transmission via on-node digital signal *** the total power consumption is reduced compared to conventional wireless sensing,the power consumption of the digital processing becomes as dominant as wireless data *** paper reviews the state-of-the-art energy-efficient digital and wireless IC design techniques for reducing the power consumption of the wireless smart sensor node to prolong battery life and enable self-powered applications.

关键词： energy-efficient digital IC wireless IC wireless smart sensing

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In vitro Cyto and Blood Compatibility of Titanium Containing Diamond-Like Carbon Prepared by Hybrid Sputtering Method

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Plasma Science and Technology 2012年第9期14卷 829-836页

作者： Krishnasamy NAVANEETHA PANDIYARAJ Jan HEEG Andreas LAMPKA Fabian JUNGE Torsten BARFELS Marion WIENECKE Young Ha RHEE hyoung Woo kim Department of Physics Sri Shakthi Institute of Engineering and Technology Institute of Surface and Thin Film Technology Hochschule WismarGermany Department of Microbiology College of Bioscience&BiotechnologyChungnam National University

In recent years, diamond-like carbon films （DLC） have been given more attention in research in the biomedical industry due to their potential application as surface coating on biomedical materials such as metals and polymer substrates. There are many ways to prepare metal containing DLC films deposited on polymeric film substrates, such as coatings from car- bonaceous precursors and some means that incorporate other elements. In this study, we in- vestigated both the surface and biocompatible properties of titanium containing DLC （Ti-DLC） films. The Ti-DLC films were prepared on the surface of poly （ethylene terephthalate）（PET） film as a function of the deposition power level using reactive sputtering technique. The films＇ hydrophilicity was studied by contact angle and surface energy tests. Their surface morphology was studied by scanning electron microscopy （SEM） and atomic force microscopy （AFM）. Their elemental chemical composition was analyzed using energy dispersive X-spectra （EDX） and X-ray photoelectron spectroscopy （XPS）. Their blood and cell compatibility was studied by in vitro tests, including tests on platelet adhesion, thrombus formation, whole blood clotting time and osteoblast cell compatibility. Significant changes in the morphological and chemical composition of the Ti-DLC films were observed and found to be a function of the deposition level. These morphological and chemical changes reduced the interfacial tension between Ti-DLC and blood proteins as well as resisted the adhesion and activation of platelets on the surface of the Ti-DLC films. The cell compatibility results exhibited significant growth of osteoblast cells on the surface of Ti incorporated DLC film compared with that of DLC film surface.

关键词： Ti-DLC hybrid reactive sputtering technique surface analysis cyto and blood compatibility

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Adaptive immunity of materials: Implications for tissue healing and regeneration

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Bioactive Materials 2024年第11期41卷 499-522页

作者： Jung-Hwan Lee Seong-Jin Shin Jun Hee Lee Jonathan CKnowles Hae-hyoung Lee Hae-Won kim Institute of Tissue Regeneration Engineering(ITREN) Dankook UniversityCheonan 31116Republic of Korea Department of Biomaterials Science College of DentistryDankook UniversityCheonan 31116Republic of Korea Department of Nanobiomedical Science and BK21 NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonan 31116Republic of Korea Cell&Matter Institute Dankook UniversityCheonan 31116Republic of Korea Mechanobiology Dental Medicine Research Center Dankook UniversityCheonan 31116Republic of Korea UCL Eastman-Korea Dental Medicine Innovation Centre Dankook UniversityCheonan 31116Republic of Korea UCL Eastman Dental Institute University College LondonLondon NW32PXUnited Kingdom

Recent cumulative findings signify the adaptive immunity of materials as a key agenda in tissue healing that can improve regenerative events and outcomes. Modulating immune responses, mainly the recruitment and functions of T and B cells and their further interplay with innate immune cells (e.g., dendritic cells, macrophages) can be orchestrated by materials. For instance, decellularized matrices have been shown to promote muscle healing by inducing T helper 2 (Th2) cell immunity, while synthetic biopolymers exhibit differential effects on B cell responses and fibrosis compared decellularized matrices. We discuss the recent findings on how implantable materials instruct the adaptive immune events and the subsequent tissue healing process. In particular, we dissect the materials’ physicochemical properties (shape, size, topology, degradation, rigidity, and matrix dynamic mechanics) to demonstrate the relations of these parameters with the adaptive immune responses in vitro and the underlying biological mechanisms. Furthermore, we present evidence of recent in vivo phenomena, including tissue healing, cancer progression, and fibrosis, wherein biomaterials potentially shape adaptive immune cell functions and in vivo outcomes. Our discussion will help understand the materials-regulated immunology events more deeply, and offer the design rationale of materials with tunable matrix properties for accelerated tissue repair and regeneration.

关键词： Adaptive immunity Materials interactions Tissue healing Physicochemical properties Immune cell interplay

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Materials and extracellular matrix rigidity highlighted in tissue damages and diseases:Implication for biomaterials design and therapeutic targets

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Bioactive Materials 2023年第2期20卷 381-403页

作者： Jae Hee Park Seung Bin Jo Jung-Hwan Lee Hae-hyoung Lee Jonathan CKnowles Hae-Won kim Institute of Tissue Regeneration Engineering(ITREN) Dankook UniversityCheonan31116Republic of Korea Department of Nanobiomedical Science and BK21 NBM Global Research Center for Regenerative Medicine Dankook UniversityCheonan31116Republic of Korea Department of Biomaterials Science College of DentistryDankook UniversityCheonan31116Republic of Korea Cell&Matter Institute Dankook UniversityCheonan31116Republic of Korea Mechanobiology Dental Medicine Research Center Dankook UniversityCheonan31116Republic of Korea UCL Eastman-Korea Dental Medicine Innovation Centre Dankook UniversityCheonan31116Republic of Korea Division of Biomaterials and Tissue Engineering UCL Eastman Dental InstituteUniversity College LondonRoyal Free HospitalRowland Hill StreetLondonNW32PFUnited Kingdom

Rigidity(or stiffness)of materials and extracellular matrix has proven to be one of the most significant extracellular physicochemical cues that can control diverse cell behaviors,such as contractility,motility,and spreading,and the resultant pathophysiological *** 2D materials engineered with tunable rigidity have enabled researchers to elucidate the roles of matrix biophysical cues in diverse cellular events,including migration,lineage specification,and mechanical ***,the recent findings accumulated under 3D environments with viscoelastic and remodeling properties pointed to the importance of dynamically changing rigidity in cell fate control,tissue repair,and disease ***,here we aim to highlight the works related with material/matrix-rigidity-mediated cell and tissue behaviors,with a brief outlook into the studies on the effects of material/matrix rigidity on cell behaviors in 2D systems,further discussion of the events and considerations in tissue-mimicking 3D conditions,and then examination of the in vivo findings that concern material/matrix *** current discussion will help understand the material/matrix-rigidity-mediated biological phenomena and further leverage the concepts to find therapeutic targets and to design implantable materials for the treatment of damaged and diseased tissues.

关键词： Matrix rigidity Biophysical cue Cell and tissue engineering 3D biomaterials conditions Therapeutic targets

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A surgical technique using the gastroepiploic vein for portal inflow restoration in living donor liver transplantation in a patient with diffuse portomesenteric thrombosis

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Hepatobiliary & Pancreatic Diseases International 2023年第5期22卷 537-540页

作者： Sang-Hoon kim Deok-Bog Moon Woo-hyoung Kang Dong-Hwan Jung Sung-Gyu Lee Division of Hepatobiliary Surgery and Liver Transplantation Department of SurgeryAsan Medical Center and University of Ulsan College of Medicine88 Olympic-ro 43-gilSongpa-guSeoul 05505Korea

Portal vein thrombosis(PVT)is no longer a definitive contraindication in liver transplants(LTs)[1].Complex vascular reconstructions such as cavoportal hemitransposition(CPHT)[2–5],renoportal anastomosis(RPA)[6,7],and use of sizable collaterals(pericholedochal varix[8,9],coronary vein,peripancreatic or perigastroesophageal varices[10],right superior colic vein[11],ileocolic vein[12],and left gastric vein[13]),or combined liverpancreas-small bowel transplant[14]are required for portal inflow in patients with total portosplenomesenteric thrombosis.

关键词： thrombosis anastomosis portal

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Anode surface engineering of zinc-ion batteries using tellurium nanobelt as a protective layer for enhancing energy storage performance

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Journal of Energy Chemistry 2024年第5期92卷 113-123页

作者： Soobeom Lee Yeonjin Je Boeun Seok Hyun Tae kim Yong-Ryun Jo Soong Ju Oh Byoungyong Im Dae Guen kim Sang-Soo Chee Geon-hyoung An Department of Energy System Engineering Gyeongsang National UniversityJinju 52725Republic of Korea Nano Convergence Materials Center Korea Institute of Ceramic Engineering and Technology(KICET)Jinju 52851Republic of Korea Department of Materials Science and Engineering Korea UniversitySeoul 02841Republic of Korea School of Materials Science and Engineering Gyeongsang National UniversityJinju 52828Republic of Korea GIST Advanced Insitute of Instrumental Analysis(GAIA) Electron Microscopy LaboratoryGwangju Institute of Science and TechnologyGwangju 61005Republic of Korea Center for Advanced Materials and Processing Institute for Advanced EngineeringYongin 17180Republic of Korea Department of Energy Engineering Gyeongsang National UniversityJinju 52725Republic of Korea

Over the years,zinc-ion batteries(ZIBs)have attracted attention as a promising next-generation energy storage technology because of their excellent safety,long cycling performance,eco-friendliness,and high-power ***,issues,such as the corrosion and dissolution of the Zn anode,limited wet-tability,and lack of sufficient nucleation sites for Zn plating,have limited their practical *** introduction of a protective layer comprising of tellurium(Te)nanobelts onto the surface of Zn anode has emerged as a promising approach to overcome these limitations and improve the electrochemical behav-ior by enhancing the safety and wettability of ZIBs,as well as providing numerous nucleation sites for Zn *** the presence of a Te-based protective layer,the energy power density of the surface-engineered Zn anode improved significantly(ranging from 310 to 144 W h kg^(-1),over a power density range of 270 to 1,800 W kg^(-1)),and the lifespan capability was *** results demonstrate that the proposed strategy of employing Te nanobelts as a protective layer holds great promise for enhancing the energy storage performance of zIBs,making them even more attractive as a viable energy storage solution forthefuture.

关键词： Zn ion battery Anode Protective layer Tellurium Nanobelt

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Brain metabolism in patients with vegetative state after post-resuscitated hypoxic-ischemic brain injury： statistical parametric mapping analysis of F-18 fluorodeoxyglucose positron emission tomography

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Chinese Medical Journal 2013年第5期126卷 888-894页

作者： Yong Wook kim hyoung Seop kim Young-Sil An Department and Research Institute of Rehabilitation Medicine Yonsei University College of Medicine Seoul Republic of Korea Department of Physical Medicine and Rehabilitation NationalHealth Insurance Corporation Ilsan Hospital Gyonggi-doRepublic of Korea Department of Nuclear Medicine Ajou University School ofMedicine Suwon Republic of Korea

Background Hypoxic-ischemic brain injury （HIBI） after cardiopulmonary resuscitation is one of the most devastating neurological conditions that causing the impaired consciousness. However, there were few studies investigated the changes of brain metabolism in patients with vegetative state （VS） after post-resuscitated HIBI. This study aimed to analyze the change of overall brain metabolism and elucidated the brain area correlated with the level of consciousness （LOC） in patients with VS after post-resuscitated HIBI. Methods We consecutively enrolled 17 patients with VS after HIBI, who experienced cardiopulmonary resuscitation. Overall brain metabolism was measured by F-18 fluorodeoxyglucose positron emission tomography （F-18 FDG PET） and we compared regional brain metabolic patterns from 17 patients with those from 15 normal controls using voxel-by-voxel based statistical parametric mapping analysis. Additionally, we correlated the LOC measured by the JFK-coma recovery scale-revised of each patient with brain metabolism by covariance analysis. Results Compared with normal controls, the patients with VS after post-resuscitated HIBI revealed significantly decreased brain metabolism in bilateral precuneus, bilateral posterior cingulate gyrus, bilateral middle frontal gyri, bilateral superior parietal gyri, bilateral middle occipital gyri, bilateral precentral gyri （PFEw correctecd 〈0.0001 ）, and increased brain metabolism in bilateral insula, bilateral cerebella, and the brainstem （PFEWcorrectecd 〈0.0001 ）. In covariance analysis, the LOC was significantly correlated with brain metabolism in bilateral fusiform and superior temporal gyri （P uncorrected 〈0.005）. Conclusions Our study demonstrated that the precuneus, the posterior cingulate area and the frontoparietal cortex, which is a component of neural correlate for consciousness, may be relevant structure for impaired consciousness in patient with VS after post-resuscitated HIBI. In post-resuscitated HIBI, measuremen

关键词： hypoxic-ischemic brain injury vegetative state brain metabolism

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