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Signal transduction of bombesin-induced circular smooth muscle cell contraction in cat esophagus

World Journal of Gastroenterology 2006年第14期12卷 2259-2263页

作者： Sung-Uk Park Chang-Yell Shin Jung-Su Ryu hyen-o la Sun-Young Park Hyun-Ju Song Young-Sil Min Dong-Seok Kim Uy-Dong Sohn Department of Pharmacology College of Pharmacy Chung Ang UniversitySeoul 156-756Republic of Korea Department of Pharmacology College of Pharmacy Chung Ang UniversitySeoul 156-756Republic of Korea Research LaboratoryDong-A Pharm. Co. LtdYongin 449-905Kyunggi-DoRepublic of Korea Department of Pharmacology College of Medicine The Catholic University of KoreaSeoul 137-701Republic of Korea Research Division for Human Life Sciences Seoul National University 28 Yongon-DongChongno-GuSeoul 110-744Republic of Korea

AIM： To investigate the mechanism of bombesin-induced circular smooth muscle cell contraction in cat esophagus. METHoDS： Specific G protein or phospholipase C involved in cat esophagus contraction was identified, muscle cells were permeabilized with saponin. After per- meabilization of muscle cells, the Gi3 antibody inhibited bombesin-induced smooth muscle cell contraction. RESULTS： Incubation of permeabilized circular muscle cells with PLC-β3 antibody could inhibit bombesin-induced contraction. H-7, chelerythrine （PKC inhibitor） and genistein （protein tyrosine kinase inhibitor） inhibited bombesin-induced contraction, but DAG kinase inhibitor, R59949, could not inhibit it. To examine which mitogen-activated protein kinase （MAPK） was involved in bombesin-induced contTaction, the specific MAPK inhibitors （MEK inhibitor, PD98059 and p38 MAPK inhibitor, SB202190） were used. Preincubation of PD98059 blocked the contraction induced by bombesin in a concentration-dependent manner. However, SB202190 had no effects on contraction. CoNCLUSIoN： Bombesin-induced circular muscle cell contraction in cat esophagus is mediated via a PKC or a PTK-dependent pathway or p44/p42 HAPK pathway.

关键词： Bombesin G protein Phospholipase C Protein kinase C Protein tyrosine kinase MAP Kinase Cell contraction

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