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Chinese Journal of Natural Medicines 2020年第10期18卷 793-800页

作者： lee Se-Eun OKHLOPKOVA Zhanna lim Chiyeon CHO Suin School of Korean Medicine Pusan National UniversityYangsan-si 50612Republic of Korea Department of Biology North-Eastern Federal UniversityYakutsk 677027Russia College of Medicine Dongguk UniversityGyeonggi-do 10326Republic of Korea

Dracocephalum palmatum Stephan is a medicinal plant traditionally used by nomadic people in Eastern Russia;however,research on this plant is currently ***,although studies have been conducted on the constituents of this plant and their antioxidant effects,data on its various pharmacological activities are still ***,this study examined the anticancer potential of the dried leaves of *** S.(DpL)using human prostate cancer PC-3 *** antioxidant potential of DpL was evaluated by estimating the total flavonoid and total phenolic content(TFC and TPC,respectively).Additionally,we investigated the effects of the DpL ethyl acetate fraction(DpLE)on cell proliferation,intracellular reactive oxygen species(ROS)generation,apoptosis,and cell cycle arrest in this cell *** expression levels of superoxide dismutase(SOD)-1,SOD-2,B-cell lymphoma 2(Bcl-2)and Bcl-2 associated X(Bax)ratio,phospho-protein kinase B(p-AKT),cleaved caspase-8,poly adenosine diphosphate(ADP)ribose polymerase(PARP),and cleaved-PARP were evaluated by western *** results indicated that DpLE causes apoptosis and exerts intracellular ROS-independent anticancer effects on prostate cancer cells,associated with increased SOD-2,cleaved caspase-8,and cleaved-PARP expression and inhibited p-AKT ***,DpLE may be a potential resource for the development of promising chemotherapeutic agents for prostate cancer.

关键词： Dracocephalum palmatum Stephan Prostate cancer Apoptosis Caspase-8

Code generation and recognition using a modified ejection system in die-casting process

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China Foundry 2020年第5期17卷 364-371页

作者： Jaekyung Song Jeongsu lee Young Chul lee Byoung Jun lee Chaeho lim Industrial Materials and Smart Manufacturing Engineering Korea University of Science and TechnologyDaejeon CityRepublic of Korea Digital Manufacturing Process Group Korea Institute of Industrial TechnologyGyeonggi-doRepublic of Korea

A code-generation and recognition technology that uses a modified ejection system in the diecasting process is *** achieve the highest level of quality management,the first requirement in the manufacturing process is to establish a product management system according to the specific product ***,a method to individually identify each product,such as a barcode or QR code,is required during the production *** manufactured in the die-casting process always have ejector pin(EP)***,an ejection system was modified to generate a unique code using EP *** ejection system has two features:an EP with a modified head to show the direction of rotation,and a function to dependently rotate EPs(five or six EPs)with a constant *** EPs are numbered according to the rotation ***,the EP marks can be viewed as a five-or six-digit code.A program was also developed to individually identify the products by automatically detecting and reading the EPs using deep learning-based object detection and classification technology.

关键词： code recognition die-casting ejection system object detection product management smart manufacturing

Effect of extracts from Stachys sieboldii Miq. on cellular reactive oxygen species and glutathione production and genomic DNA oxidation

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Asian Pacific Journal of Tropical Biomedicine 2018年第10期8卷 485-489页

作者： JW lee W Wu SY lim Division of Marine Bioscience Korea Maritime and Ocean University Department of Marine Bio-Pharmacology Shanghai Ocean University

Objective: To evaluate the antioxidant activity of extracts and fractions from Stachys sieboldii Miq., and to examine its effect on the cellular reactive oxygen species(ROS) and glutathione(GSH) production and genomic DNA oxidation in HT-1080 cells. Methods: The ROS generation induced by H2 O2 was measured by the dichlorofluorescein-diacetate assay. GSH levels were measured using a fluorescent method with mBBr. Genomic DNA oxidative damage was measured with levels of oxidative DNA induced by the reaction of ferritin with H2 O2. Results: The n-hexane, 85% aqueous methanol and n-butanol fractions(0.05 mg/mL concentrations) inhibited H2 O2-induced ROS generation by 63%, 35% and 45%, respectively. GSH levels were significantly increased in both acetone+methylene chloride and methanol extracts(P<0.05). Supplementation of cells with n-hexane significantly increased GSH levels at concentrations of 0.05 mg/mL(P<0.05). Both the acetone+methylene chloride and methanol extracts, as well as all fractions significantly inhibited oxidative DNA damage(P<0.05). Conclusions: These results indicate that cellular oxidation was inhibited by the n-hexane fraction and this fraction may contain valuable active compounds.

关键词： Stachys sieboldii Miq. Reactive oxygen species Glutathione DNA oxidation Antioxidant

Is cholecystectomy a reasonable treatment option for simple gallbladder polyps larger than 10 mm?

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World Journal of Gastroenterology 2015年第14期21卷 4248-4254页

作者： hye Yon Park Se Hoon Oh Kwang Hyuck lee Jong Kyun lee Kyu Taek lee Division of Gastroenterology Department of Internal MedicineSamsung medical centerSungkyunkwan University School of Medicine

AIM: To determine the relevance of the 10-mm size criterion of the generally accepted surgical indication for gallbladder polyps(GBPs).METHODS: We collected data of patients who were confirmed to have GBPs through cholecystectomy at Samsung Medical Center between January 1997 andDecember *** the patients who underwent cholecystectomy for GBP, those with a definite evidence for malignancy such as adjacent organ invasion, metastasis on preoperative imaging studies, polyp larger than 20 mm, absence of preoperative imaging study results, and patients having gallstones were *** retrospectively collected and analyzed information on patient's clinical characteristics, symptoms, ultrasonographic findings, and blood laboratory ***: A total of 836 patients who had undergone cholecystectomy were retrospectively *** hundred eighty patients(93%) had benign polyps, whereas 56 patients(7%) had malignant *** the 56 patients with malignancy, 4 patients(7%) had borderline GBP(10-12 mm) and a patient had small GBP(< 10 mm) with T2 *** conducted an ROC curve analysis to verify the 10-mm size criteria(AUC = 0.887, SD = 0.21, P < 0.001).In the ROC curve for polyp size and malignancy, sensitivity and specificity of the 10-mm size criterion was 98.2% and 19.6%, *** specificity of the 11-mm and 12-mm size criteria was 44.6% and 56%, respectively, whereas the sensitivity of these two size criteria was *** defined the GBPs of 10 to 12 mm as a borderline-sized GBP, which were found in 411 patients(49%).In this group, there was a significant difference in age between patients with benign and malignant GBPs(47 years vs 60 years, P < 0.05).CONCLUSION: GBPs larger than 13 mm need immediate excision whereas for borderline-sized GBPs detected in young patients, careful medical observation can be a rational decision.

关键词： Gallbladder polyp Gallbladder cancer Cholecystecto

Transcriptome sequencing assisted discovery and computational analysis of novel SNPs associated with flowering in Raphanus sativus in-bred lines for marker-assisted backcross breeding

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Horticulture Research 2019年第1期6卷 248-259页

作者： Jinhee Kim Abinaya Manivannan Do-Sun Kim Eun-Su lee hye-Eun lee Vegetable Research Division National Institute of Horticultural and Herbal ScienceRural Development AdministrationJeonju 55365Republic of Korea

The sequencing of radish genome aids in the better understanding and tailoring of traits associated with economic *** order to accelerate the genomics assisted breeding and genetic selection,transcriptomes of 33 radish inbred lines with diverse traits were sequenced for the development of single nucleotide polymorphic(SNP)*** sequence reads ranged from 2,560,543,741 bp to 20,039,688,139 bp with the GC(%)of 47.80–49.34 and phred quality score(Q30)of 96.47–97.54%.A total of 4951 polymorphic SNPs were identified among the accessions after stringent filtering and 298 SNPs with efficient marker assisted backcross breeding(MAB)markers were generated from the polymorphic ***,functional annotations of SNPs revealed the effects and importance of the SNPs identified in the flowering *** SNPs were predominantly associated with the four major flowering related transcription factors such as MYB,MADS box(AG),AP2/EREB,and *** addition,SNPs in the vital flowering integrator gene(FT)and floral repressors(EMBRYONIC FLOWER 1,2,and FRIGIDA)were identified among the radish inbred ***,50 SNPs were randomly selected from 298 SNPs and validated using Kompetitive Allele Specific PCR genotyping system(KASP)in 102 radish inbred *** homozygosity of the inbred lines varied from 56 to 96%and the phylogenetic analysis resulted in the clustering of inbred lines into three *** together,the SNP markers identified in the present study can be utilized for the discrimination,seed purity test,and adjusting parental combinations for breeding in radish.

关键词： breeding Raphanus utilized

Development of CAD/CAM system and profile measuring device for CNC grinding machine to obtain an optimal grinding speed

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Journal of Central South University 2011年第1期18卷 146-152页

作者： lim Sang-Heon KIM Jae-Hyun lee Choon-Man Agency of Promoting Technology Innovation Gyeongnam TechnoparkChangwon 641-465Korea School of Mechatronics Changwon National University

Cam mechanics is one of the most popular devices for generating irregular motions and is widely used in automatic equipment,such as textile machines,internal combustion engines,and other automatic *** order to obtain a positive motion from the follower using a rotating cam,its shape should be correctly designed and *** development of an adequate CAD/CAM system for a cam profile CNC grinding machine is necessary to manufacture high-precision *** purpose of this study is the development of a CAD/CAM system and profile measuring device for a CNC grinding machine to obtain an optimal grinding speed with a constant surface *** types of disk cams were manufactured using the proposed algorithm and procedures to verify effectiveness of the developed CAD/CAM system.

关键词： cam CAD/CAM system CNC grinding machine optimal grinding speed

Improvement of light uniformity by lighting arrangement for standardized crop production

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Journal of Central South University 2014年第11期21卷 4311-4319页

作者： lee Hwa-Soo Kwon Sook-Youn lim Jae-Hyun Department of Computer Science and Engineering Kongju National UniversityCheonan 331-717 Korea

In a commercialized, fully artificial plant factory, artificial luminaire is arranged in a unified way using a general illumination theory, an actual measurement, or an empirical methodology. However, with these methods, lightings are implemented without considering specific optical characteristics of lighting or material characteristics of each component that constructs a cultivation system, resulting in an amount of light that becomes irregular. The amount of lighting is closely related with the growth and quality of crops, and the deviation between points where cultivated crops are located causes quality difference in the produced crops, thus impairing the economic feasibility of a plant factory. In this regard, a simulation to figure out an optimum lighting layout was performed. Arrangements based on the spectrum distribution of light source and reflector materials were implemented to ascertain the distance between lighting and height of lighting and gather information in the pre-treatment process to improve the uniformity of light in the plant cultivation system. Improvement of around 15% in light uniformity is achieved compared with the existing system after the simulation is carried out. This result would reduce the deviation in crop growth to make uniform quality crop production possible.

关键词： high-quality crop light uniformity improvement optimal lighting layout fully artificial plant factory Relux simulation

Gene editing of authentic Brassica rapa flavonol synthase 1 generates dihydroflavonol-accumulating Chinese cabbage

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Horticulture Research 2023年第12期10卷 58-69页

作者： Sangkyu Park Hyo lee Jaeeun Song Chan Ju lim Jinpyo Oh Sang Hoon lee Saet Buyl lee Jong-Yeol lee Sunhyung lim Jin A.Kim Beom-Gi Kim Metabolic Engineering Division National Institute of Agricultural SciencesRural Development AdministrationJeonju54874South Korea Institute of Biotechnology and Breeding Asiaseed Inc.Icheon17414South Korea Food and Nutrition Division National Institute of Agricultural SciencesRural Development AdministrationWanju55365South Korea Department of Horticultural Biotechnology School of BiotechnologyHankyong National UniversityAnseong17579South Korea

Flavonols are the major class of flavonoids of green Chinese cabbage(Brassica rapa ***).The *** genome harbors seven flavonol synthase genes(BrFLSs),but they have not been functionally ***,transcriptome analysis showed four BrFLSs mainly expressed in Chinese *** them,only BrFLS1 showed major FLS activity and additional flavanone 3β-hydroxylase(F3H)activity,while BrFLS2 and BrFLS3.1 exhibited only marginal F3H *** generated BrFLS1-knockout(BrFLS1-KO)Chinese cabbages using CRISPR/Cas9-mediated genome editing and obtained transgene-free homozygous plants without off-target mutation in the T1 generation,which were further advanced to the T2 generation showing normal ***-ESI-QTOF-MS analysis revealed that flavonol glycosides were dramatically decreased in the T2 plants,while dihydroflavonol glycosides accumulated concomitantly to levels corresponding to the reduced levels of *** PCR analysis revealed that the early steps of phenylpropanoid and flavonoid biosynthetic pathway were upregulated in the BrFLS1-KO *** accordance,total phenolic contents were slightly enhanced in the BrFLS1-KO plants,which suggests a negative role of flavonols in phenylpropanoid and flavonoid biosynthesis in Chinese *** surveys revealed that the BrFLS1-KO Chinese cabbages showed normal head formation and reproductive phenotypes,but subtle morphological changes in their heads were *** addition,their seedlings were susceptible to osmotic stress compared to the controls,suggesting that flavonols play a positive role for osmotic stress tolerance in *** *** this study,we showed that CRISPR/Cas9-mediated BrFLS1-KO successfully generated a valuable breeding resource of Chinese cabbage with distinctive metabolic traits and that CRISPR/Cas9 can be efficiently applied in functional Chinese cabbage breeding.

关键词： steps slightly phenolic

Design and use of group-specific primers and probes for real-time quantitative PCR

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Frontiers of Environmental Science & Engineering 2011年第1期5卷 28-39页

作者： Juntaek lim Seung Gu SHIN Seungyong lee Seokhwan HWANG School of Environmental Science and Engineering Pohang University of Science and TechnologyPohangGyungbuk 790-784The Republic of Korea

Real-time quantitative polymerase chain reaction(qPCR)has gained popularity as a technique to detect and quantify a specific group of target microorganisms from various environmental samples including soil,water,sediments,and *** qPCR is a very useful technique for nucleic acid quantification,accurately quantifying the target microbial group strongly depends on the quality of the primer and probe *** aspects of conducting qPCR assays have become increasingly routine and automated;however,one of the most important aspects,designing and selecting primer and probe sets,is often a somewhat arcane *** many cases,failed or non-specific amplification can be attributed to improperly designed primer-probe *** paper is intended to provide guidelines and general principles for designing group-specific primers and probes for qPCR *** demonstrate the effectiveness of these guidelines by reviewing the use of qPCR to study anaerobic processes and biologic nutrient removal *** assays using group-specific primers and probes designed with this method,have been used to successfully quantify 16S ribosomal Ribonucleic Acid(16S rRNA)gene copy numbers from target methanogenic and ammoniaoxidizing bacteria in various laboratory-and full-scale biologic *** with a good command of primer and probe design can use qPCR as a valuable tool to study biodiversity and to develop more efficient control strategies for biologic processes.

关键词： absolute quantification design guideline primer probe real-time quantitative polymerase chain reaction(qPCR)

Complete remission of advanced hepatocellular carcinoma by radiofrequency ablation after sorafenib therapy

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World Journal of Gastroenterology 2015年第8期21卷 2568-2572页

作者： Jung Gil Park Soo Young Park hye Won lee Division of Gastroenterology and Hepatology Department of Internal Medicine CHA University Gumi CHA Medical Center Division of Gastroenterology and Hepatology Department of Internal Medicine School of Medicine Kyungpook National University Department of Pathology School of Medicine Kyungpook National University

Sorafenib,a potent multikinase inhibitor,lead to a significant improvement in progression free survival and overall survival in patients with advanced hepatocellular carcinoma(HCC).Though sorafenib has proven its efficacy in advanced stage HCC,there are limitedreports on the role of sorafenib allowing for curative treatment by *** herein report a case of advanced HCC with vascular invasion,which showed treatment response by sorafenib therapy as to allow for radiofrequency ablation as curative *** patient was followed-up for 6 mo without recurrence with continued sorafenib therapy.

关键词： Hepatocellular carcinoma Radiofrequency ablation S